Standard name | |
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Description | MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 4 | 1 | 1 | 1 |
Bud | 0 | 3 | 2 | 1 | 0 | 1 | 2 | 4 | 6 | 5 | 4 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 7 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 0 | 3 | 2 | 3 | 1 | 2 | 3 | 3 | 2 | 1 | 0 | 4 | 1 | 9 | 6 | 8 | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 160 | 492 | 256 | 148 | 249 | 349 | 329 | 290 | 221 | 247 | 162 | 192 | 230 | 349 | 365 | 312 | 159 | 121 | 159 | 467 | 236 | 96 | 313 | 274 |
Endoplasmic Reticulum | 1 | 3 | 1 | 2 | 0 | 0 | 3 | 1 | 4 | 2 | 4 | 0 | 2 | 0 | 1 | 27 | 13 | 12 | 0 | 3 | 2 | 0 | 1 | 1 |
Endosome | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 2 | 1 | 6 | 2 | 1 | 1 | 2 |
Golgi | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 5 | 3 | 0 | 1 | 1 |
Mitochondria | 6 | 10 | 4 | 14 | 11 | 108 | 171 | 141 | 176 | 231 | 169 | 187 | 0 | 1 | 2 | 7 | 1 | 4 | 0 | 4 | 6 | 1 | 3 | 2 |
Nucleus | 0 | 0 | 1 | 1 | 0 | 5 | 0 | 1 | 1 | 1 | 0 | 2 | 0 | 2 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 0 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 3 | 1 | 3 | 1 |
Vac/Vac Membrane | 0 | 0 | 0 | 1 | 2 | 6 | 5 | 5 | 2 | 8 | 2 | 5 | 0 | 1 | 0 | 3 | 2 | 0 | 0 | 3 | 2 | 0 | 0 | 2 |
Unique Cell Count | 166 | 502 | 258 | 154 | 255 | 389 | 427 | 368 | 326 | 379 | 263 | 291 | 232 | 350 | 366 | 324 | 162 | 127 | 167 | 500 | 271 | 105 | 343 | 302 |
Labelled Cell Count | 168 | 511 | 264 | 171 | 265 | 473 | 516 | 447 | 416 | 497 | 344 | 401 | 232 | 357 | 372 | 365 | 184 | 148 | 167 | 500 | 271 | 105 | 343 | 302 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.2 | 5.2 | 4.6 | 4.4 | 4.3 | 3.9 | 3.2 | 3.7 | 3.3 | 3.0 | 3.3 | 3.1 | 5.3 | 5.4 | 5.5 | 5.8 | 6.8 | 6.9 | 4.8 | 5.4 | 6.0 |
Std Deviation (1e-4) | 0.4 | 0.6 | 1.0 | 1.3 | 1.0 | 1.4 | 0.6 | 1.4 | 1.0 | 0.7 | 1.5 | 0.7 | 1.2 | 1.4 | 1.3 | 1.5 | 1.3 | 1.3 | 1.3 | 2.1 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.07 | -0.08 | -0.25 | -0.51 | -0.32 | -0.49 | -0.61 | -0.47 | -0.57 | 0.21 | 0.24 | 0.25 | 0.33 | 0.56 | 0.59 | 0.07 | 0.24 | 0.39 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -2.2 | -1.4 | -4.8 | -8.0 | -7.5 | -9.8 | -10.4 | -10.8 | -10.0 | -0.1 | 0.9 | 0.9 | -2.3 | -1.0 | -2.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.4 | 4.2 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |