Standard name
Description MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Cytoplasm 0.96 0.98 0.99 0.96 0.98 0.9 0.77 0.79 0.68 0.65 0.62 0.66 0.99 1.0 1.0 0.96 0.98 0.95 0.96 0.93 0.87 0.92 0.91 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.08 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.09 0 0.28 0.4 0.38 0.54 0.61 0.64 0.64 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 4 1 1 1
Bud 0 3 2 1 0 1 2 4 6 5 4 7 0 0 0 0 0 0 0 2 0 0 7 6
Bud Neck 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1
Bud Site 0 0 0 0 0 0 1 0 0 0 0 2 0 0 0 0 0 0
Cell Periphery 1 1 0 3 2 3 1 2 3 3 2 1 0 4 1 9 6 8 0 0 0 0 0 1
Cytoplasm 160 492 256 148 249 349 329 290 221 247 162 192 230 349 365 312 159 121 159 467 236 96 313 274
Endoplasmic Reticulum 1 3 1 2 0 0 3 1 4 2 4 0 2 0 1 27 13 12 0 3 2 0 1 1
Endosome 0 0 0 1 1 1 1 1 0 0 0 1 0 0 0 2 0 2 1 6 2 1 1 2
Golgi 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 1 5 3 0 1 1
Mitochondria 6 10 4 14 11 108 171 141 176 231 169 187 0 1 2 7 1 4 0 4 6 1 3 2
Nucleus 0 0 1 1 0 5 0 1 1 1 0 2 0 2 3 1 1 0 0 0 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 1 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 1 3 0 1 2 0 0 0 1 0 1 0 1 3 1 3 1
Vac/Vac Membrane 0 0 0 1 2 6 5 5 2 8 2 5 0 1 0 3 2 0 0 3 2 0 0 2
Unique Cell Count 166 502 258 154 255 389 427 368 326 379 263 291 232 350 366 324 162 127 167 500 271 105 343 302
Labelled Cell Count 168 511 264 171 265 473 516 447 416 497 344 401 232 357 372 365 184 148 167 500 271 105 343 302


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 5.2 4.6 4.4 4.3 3.9 3.2 3.7 3.3 3.0 3.3 3.1 5.3 5.4 5.5 5.8 6.8 6.9 4.8 5.4 6.0
Std Deviation (1e-4) 0.4 0.6 1.0 1.3 1.0 1.4 0.6 1.4 1.0 0.7 1.5 0.7 1.2 1.4 1.3 1.5 1.3 1.3 1.3 2.1 1.7
Intensity Change (Log2) -0.07 -0.08 -0.25 -0.51 -0.32 -0.49 -0.61 -0.47 -0.57 0.21 0.24 0.25 0.33 0.56 0.59 0.07 0.24 0.39


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.2 -1.4 -4.8 -8.0 -7.5 -9.8 -10.4 -10.8 -10.0 -0.1 0.9 0.9 -2.3 -1.0 -2.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 4.4 4.2 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mkk1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available