Standard name
Human Ortholog
Description Mitochondrial matrix cochaperone; nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; acts as oxidative sensor to regulate mitochondrial Ssc1p; in presence of oxidative stress, dimeric Mge1p becomes a monomer and unable to regulate Ssc1p function; homolog of E. coli GrpE and human Mge1 (GRPEL1), which also responds to oxidative stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.05 0.05 0.06 0.06 0 0.08 0.06 0.06 0.08 0.08 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.96 0.97 0.93 0.8 0.81 0.79 0.7 0.73 0.67 0.63 0.98 0.99 0.99 0.87 0.88 0.93 0.91 0.96 0.95 0.92 0.91 0.93
Endoplasmic Reticulum 0 0 0 0 0.05 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.26 0.07 0.14 0.24 0.31 0.28 0.29 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.1 0.08 0.06 0.05 0.06 0.11 0 0 0 0.15 0.14 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 1 1 1 4 3 5 2 8 8 0 0 0 2 0 0 2 0 0 0 1 0
Bud 0 1 0 0 1 2 2 2 3 2 7 4 0 0 0 2 0 0 1 0 0 0 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cell Periphery 1 14 4 5 11 1 17 16 10 14 30 27 9 6 7 5 4 2 0 0 0 1 2 1
Cytoplasm 182 256 76 77 181 79 174 224 119 127 245 246 184 261 368 194 124 183 198 252 95 194 290 339
Endoplasmic Reticulum 3 4 3 1 9 2 7 4 7 1 17 13 4 2 2 9 2 5 3 2 0 6 8 9
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1 0 1
Golgi 1 0 0 0 0 0 1 0 0 0 2 1 0 0 0 3 0 0 1 0 0 0 0 1
Mitochondria 2 0 0 0 0 26 16 39 41 54 103 111 3 0 1 4 2 4 3 0 0 2 6 1
Nucleus 1 1 0 1 2 0 3 1 0 1 4 5 0 0 0 3 2 8 0 0 0 0 0 0
Nuclear Periphery 1 1 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 1 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 0 0 4 4 22 24 11 9 21 43 0 0 0 33 20 13 0 2 0 2 1 0
Unique Cell Count 187 261 79 79 194 99 214 283 170 174 363 389 188 263 371 223 141 197 218 262 101 212 319 363
Labelled Cell Count 193 277 83 85 209 115 246 313 197 212 437 460 200 269 378 255 155 215 218 262 101 212 319 363


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.6 9.5 8.8 8.4 9.5 5.8 8.2 7.8 7.2 7.3 7.3 7.4 9.4 10.0 10.0 12.4 12.0 12.5 8.1 8.6 9.6
Std Deviation (1e-4) 1.8 1.3 1.3 1.2 1.6 1.2 1.8 1.6 1.6 1.6 1.4 1.5 1.4 1.5 1.7 4.1 3.8 3.6 1.3 1.5 1.8
Intensity Change (Log2) -0.07 0.11 -0.6 -0.1 -0.17 -0.29 -0.27 -0.28 -0.26 0.09 0.18 0.19 0.49 0.44 0.51 -0.12 -0.03 0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0.8 0 0 0.9 1.0 0.6 0 0 0 0 0 0
Cytoplasm 0.5 -0.9 -3.2 -3.2 -3.6 -4.7 -4.3 -5.2 -5.8 0.8 2.0 2.1 -2.3 -2.0 -1.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.9 0 3.5 4.8 5.6 5.4 5.4 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 3.0 2.7 0 0 0 3.1 0 0 0 3.6 3.5 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.3682 3.5797 3.1111 2.9959 2.5889 2.98 4.1658 6.1213 5.8385 5.2309 5.4614 5.5903 5.9216 7.6581 7.0095 6.8446 6.3888 6.7624
Actin 0.0143 0.0105 0.016 0.0038 0.0135 0.0018 0.0225 0.0004 0.0042 0.0032 0.009 0.0026 0.0012 0.0002 0.0043 0.0003 0.0018 0.0011
Bud 0.0006 0.0007 0.0051 0.0005 0.0005 0.0002 0.0009 0.0021 0.0046 0.0011 0.0012 0.0004 0.0001 0.0003 0.0006 0.0005 0.0002 0.0001
Bud Neck 0.0018 0.0003 0.0012 0.0004 0.0012 0.0013 0.0032 0.0007 0.0005 0.0005 0.0003 0.0021 0.0002 0.0001 0.0005 0.0003 0.0005 0.0015
Bud Periphery 0.0006 0.0004 0.0055 0.0003 0.0002 0.0001 0.001 0.0005 0.0014 0.0011 0.0013 0.0005 0.0001 0.0001 0.0002 0.0001 0.0001 0
Bud Site 0.0024 0.0015 0.0044 0.0009 0.0006 0.0001 0.003 0.0161 0.0032 0.0005 0.0004 0.0004 0.0003 0.0009 0.001 0.0005 0.0002 0.0001
Cell Periphery 0.0003 0.0001 0.0002 0.0001 0 0.0001 0.0004 0.0001 0.0001 0.0001 0.0001 0.0001 0.0003 0.0001 0.0002 0.0001 0.0001 0.0001
Cytoplasm 0.7956 0.9039 0.8386 0.8741 0.7726 0.8953 0.7124 0.9394 0.9339 0.864 0.9157 0.9497 0.9078 0.9693 0.9359 0.8957 0.9177 0.9581
Cytoplasmic Foci 0.0406 0.0163 0.0295 0.032 0.0425 0.0205 0.0304 0.0205 0.0119 0.0172 0.0079 0.0112 0.0133 0.0108 0.0166 0.0238 0.0227 0.0081
Eisosomes 0.0001 0 0.0001 0 0 0 0.0003 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0111 0.0044 0.0048 0.0049 0.0042 0.0114 0.0243 0.001 0.0019 0.0028 0.0073 0.0032 0.0216 0.0017 0.004 0.0037 0.0065 0.0068
Endosome 0.0508 0.0186 0.0365 0.0465 0.0693 0.0303 0.0538 0.0103 0.0137 0.0548 0.0326 0.0122 0.0135 0.0046 0.0192 0.0591 0.0279 0.0137
Golgi 0.0087 0.0054 0.0085 0.0107 0.0075 0.0035 0.014 0.0008 0.005 0.0086 0.0078 0.004 0.0027 0.0017 0.0033 0.0023 0.0028 0.0012
Lipid Particles 0.0038 0.0004 0.007 0.0026 0.0227 0.0005 0.0151 0.0002 0.0006 0.0055 0.0005 0.0013 0.0016 0.0001 0.0002 0.0002 0.0013 0.0006
Mitochondria 0.011 0.003 0.0061 0.003 0.0021 0.0014 0.0197 0.0001 0.0092 0.02 0.0064 0.0015 0.0006 0.0002 0.0005 0.0003 0.0004 0.0005
None 0.0353 0.0276 0.0168 0.0059 0.0218 0.0274 0.0258 0.0048 0.0042 0.0036 0.0025 0.0035 0.0303 0.0073 0.0091 0.0038 0.0125 0.005
Nuclear Periphery 0.004 0.0006 0.0018 0.0016 0.001 0.0007 0.0208 0.0003 0.0005 0.0013 0.0028 0.0006 0.0011 0.0002 0.0005 0.001 0.0008 0.0006
Nucleolus 0.0008 0 0.0001 0 0.0001 0 0.0007 0 0 0.0001 0 0 0 0 0 0 0 0
Nucleus 0.0023 0.0008 0.0009 0.0012 0.0009 0.0008 0.0163 0.0009 0.0009 0.0016 0.0009 0.0008 0.0009 0.0005 0.0007 0.0011 0.001 0.0007
Peroxisomes 0.0082 0.0017 0.0107 0.0042 0.0267 0.001 0.0176 0.0002 0.0012 0.0014 0.0002 0.0024 0.0014 0.0006 0.0004 0.0001 0.0003 0.0001
Punctate Nuclear 0.002 0.0003 0.0012 0.0009 0.0038 0.0002 0.0027 0.0003 0.0003 0.0005 0.0002 0.001 0.0007 0.0001 0.0002 0.0007 0.0004 0.0003
Vacuole 0.0045 0.0029 0.004 0.005 0.007 0.0026 0.0091 0.0013 0.002 0.0094 0.0018 0.0019 0.0021 0.0012 0.0022 0.0051 0.0024 0.0011
Vacuole Periphery 0.0012 0.0006 0.0011 0.0015 0.0016 0.0007 0.006 0.0002 0.0005 0.0027 0.0011 0.0007 0.0003 0.0001 0.0004 0.0012 0.0004 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 140.5355 122.0407 117.1946 120.8477 122.8619 121.9975 124.847 123.4555 131.9542 138.0746
Translational Efficiency 1.8166 1.8993 1.9138 1.693 1.8964 2.3176 2.0007 1.9156 2.0127 1.9799

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1910 657 179 1215 1035 2677 924 1381 2945 3334 1103 2596

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 789.62 950.78 1318.87 1067.88 888.19 977.90 1118.09 1098.00 824.26 972.56 1150.67 1083.90
Standard Deviation 114.91 146.28 186.23 161.29 105.74 126.43 158.58 158.57 121.28 131.03 179.38 160.56
Intensity Change Log 2 0.267953 0.740072 0.435519 0.138819 0.332096 0.305938 0.201034 0.538503 0.368374

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000341 0.000772 0.001977 0.002356 0.000347 0.000692 0.000524 0.000352 0.000343 0.000707 0.000760 0.001290
Bud Neck 0.006824 0.003217 0.003843 0.003625 0.003623 0.004746 0.001675 0.002960 0.005699 0.004445 0.002027 0.003271
Bud Site 0.020646 0.039936 0.031454 0.055014 0.020114 0.032032 0.022192 0.033041 0.020459 0.033590 0.023695 0.043325
Cell Periphery 0.000118 0.000131 0.000290 0.000202 0.000191 0.000110 0.000131 0.000071 0.000144 0.000114 0.000156 0.000132
Cytoplasm 0.667388* 0.733880* 0.607493* 0.747288* 0.685459* 0.760551* 0.831158* 0.793432* 0.673739* 0.755295* 0.794860* 0.771835*
Cytoplasmic Foci 0.167085 0.139594 0.089642 0.059542 0.207553* 0.115828 0.056529 0.056776 0.181307* 0.120512 0.061903 0.058071
Eisosomes 0.000056 0.000027 0.000029 0.000021 0.000067 0.000035 0.000010 0.000010 0.000060 0.000034 0.000013 0.000015
Endoplasmic Reticulum 0.002486 0.000829 0.001655 0.000506 0.001730 0.000448 0.000205 0.000160 0.002220 0.000523 0.000440 0.000322
Endosome 0.012595 0.011232 0.012971 0.007595 0.009591 0.007201 0.001978 0.003165 0.011540 0.007995 0.003762 0.005239
Golgi 0.010319 0.004207 0.000990 0.007394 0.009986 0.011248 0.000355 0.000506 0.010202 0.009861 0.000458 0.003730
Lipid Particles 0.002240 0.000543 0.003423 0.000751 0.001721 0.000700 0.000524 0.000352 0.002058 0.000669 0.000994 0.000539
Mitochondria 0.002501 0.001265 0.000088 0.002273 0.001200 0.002097 0.000057 0.000064 0.002044 0.001933 0.000062 0.001098
Mitotic Spindle 0.000526 0.000530 0.008480 0.011135 0.001503 0.005375 0.004293 0.006390 0.000870 0.004420 0.004972 0.008611
None 0.001416 0.000401 0.001326 0.000583 0.000577 0.001747 0.002529 0.001759 0.001121 0.001481 0.002334 0.001209
Nuclear Periphery 0.000151 0.000027 0.000495 0.000055 0.000065 0.000030 0.000062 0.000041 0.000120 0.000029 0.000133 0.000047
Nuclear Periphery Foci 0.000304 0.000751 0.010231 0.001189 0.000534 0.000143 0.003338 0.001066 0.000385 0.000263 0.004457 0.001124
Nucleolus 0.000329 0.000102 0.000118 0.000087 0.000110 0.000192 0.000096 0.000124 0.000252 0.000174 0.000099 0.000107
Nucleus 0.023995 0.009441 0.053532 0.011013 0.017517 0.008690 0.021942 0.012292 0.021718 0.008838 0.027069 0.011693
Peroxisomes 0.002107 0.002234 0.001559 0.003034 0.001198 0.002221 0.000985 0.001328 0.001787 0.002223 0.001079 0.002126
Vacuole 0.073611 0.048773 0.169882 0.084021 0.034328 0.042389 0.051209 0.085836 0.059806 0.043647 0.070468 0.084987
Vacuole Periphery 0.004961 0.002109 0.000522 0.002315 0.002585 0.003524 0.000209 0.000273 0.004126 0.003245 0.000260 0.001229

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.71 -2.35 -3.98 -2.16 -0.55 -1.72 -2.74 0.84 1.96 3.12 -2.14 -3.51 -3.70 -1.09 -0.85
Bud Neck 6.17 4.44 6.96 0.46 2.23 -2.57 5.34 2.99 5.54 -2.31 3.11 10.22 8.20 5.05 -2.04
Bud Site -5.89 -0.77 -5.35 0.56 -2.56 -6.13 -0.33 -2.28 2.21 -1.78 -8.51 -0.49 -5.43 0.96 -3.98
Cell Periphery -0.98 -6.48 -1.43 -0.75 4.37 6.03 1.93 9.53 7.01 2.80 4.19 -0.74 3.78 1.13 2.44
Cytoplasm -5.82 5.07 -3.38 2.70 -6.52 -9.12 -11.54 -8.04 0.00 3.90 -13.64 -10.54 -8.83 3.74 3.23
Cytoplasmic Foci 3.97 12.36 24.39 13.51 2.31 14.67 24.69 25.29 17.69 0.27 14.88 30.56 34.78 22.56 1.00
Eisosomes 4.13 6.58 9.33 1.00 2.26 4.42 8.50 8.76 7.55 1.73 6.07 13.63 13.48 6.26 -0.85
Endoplasmic Reticulum 2.95 2.03 4.22 1.55 4.34 4.51 5.35 5.68 4.35 3.16 5.01 5.37 5.88 2.81 2.50
Endosome 0.94 2.20 7.75 4.05 3.08 2.47 8.61 7.32 9.61 -3.04 5.44 14.80 12.06 7.52 -1.08
Golgi 8.13 16.42 5.27 0.35 -2.58 -1.01 9.74 9.61 13.77 -0.79 0.41 19.30 11.28 9.57 -2.94
Lipid Particles 7.96 -2.65 4.87 -3.20 5.27 7.19 7.19 9.93 5.10 6.24 9.43 5.24 8.77 -0.31 4.36
Mitochondria 1.62 9.44 1.64 0.10 -1.28 -1.73 2.91 2.92 6.02 0.18 0.30 9.21 3.46 2.84 -1.32
Mitotic Spindle -0.09 -3.43 -4.48 -4.47 0.98 -3.09 -2.16 -2.50 -0.42 -0.57 -4.26 -4.04 -5.11 -2.02 -0.66
None 4.03 0.62 2.82 -3.35 2.55 -5.16 -2.68 -2.72 0.65 0.99 -1.32 -1.64 0.15 1.44 1.70
Nuclear Periphery 3.04 -7.87 0.93 -9.12 9.78 2.31 -3.21 -0.70 -7.05 3.87 3.43 -3.49 1.01 -10.74 8.00
Nuclear Periphery Foci -2.50 -8.93 -10.86 -3.68 7.87 5.44 -11.77 -5.12 -8.98 8.24 2.38 -14.83 -10.80 -12.63 11.12
Nucleolus 3.48 3.06 3.05 -0.47 0.55 -2.85 0.85 -1.44 2.26 -2.77 1.81 3.50 2.98 1.92 -1.14
Nucleus 8.29 -6.80 3.55 -6.06 7.93 7.20 -7.19 0.89 -8.75 8.38 12.00 -7.85 4.00 -11.53 10.83
Peroxisomes -0.29 3.00 -0.15 0.09 -2.36 -5.70 1.86 1.06 6.12 -1.02 -2.43 4.83 1.04 2.84 -2.95
Vacuole 4.67 -9.10 -13.85 -15.83 3.74 -3.38 -10.11 -18.71 -17.61 -8.10 7.06 -8.45 -20.21 -25.54 -7.05
Vacuole Periphery 4.90 9.73 6.06 1.66 -1.84 -1.98 7.25 7.11 9.63 -1.07 2.04 12.02 9.13 7.48 -2.31