Standard name
Human Ortholog
Description Serine/threonine protein kinase; inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck; KIN4 has a paralog, FRK1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.19 0.05 0.11 0.07 0.14 0.1 0.06 – 0.06 0 0 0 0.1 0 0 0.13 0.15 0.1 0.09 0.06 0.06 0.07 0.08 0.05
Bud 0.09 0.12 0 0 0 0 0 – 0.06 0.06 0.07 0 0.09 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.13 0.1 0.11 0.11 0.07 0.09
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.42 0.15 0.4 0.46 0.23 0.24 0.14 – 0.14 0.1 0.16 0.15 0.34 0.31 0.3 0.36 0.38 0.43 0.56 0.16 0.55 0.51 0.59 0.61
Cytoplasm 0.51 0.77 0.69 0.74 0.72 0.68 0.76 – 0.69 0.74 0.64 0.74 0.76 0.91 0.92 0.54 0.49 0.48 0.08 0.39 0.14 0.13 0.13 0.14
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0.08 0.06 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 0 0 0.1 0.16 – 0.3 0.25 0.36 0.29 0 0 0 0 0.05 0.06 0 0.1 0.06 0.06 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.05 0 0.06 0 0.07 0 0 – 0 0 0 0 0 0 0 0.07 0.08 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 28 18 30 16 45 33 26 – 20 10 13 11 31 9 7 42 30 19 15 24 20 25 33 26
Bud 14 41 11 10 13 11 14 – 21 22 28 16 27 12 8 10 3 3 2 13 6 7 2 9
Bud Neck 0 1 7 0 6 4 0 – 2 2 3 1 0 0 0 0 1 0 23 39 36 38 31 44
Bud Site 4 3 4 3 4 4 5 – 2 2 0 1 6 0 1 3 3 3 – – – – – –
Cell Periphery 63 51 112 108 74 75 66 – 52 36 67 58 102 124 132 112 76 79 101 65 190 177 259 315
Cytoplasm 77 255 194 172 231 215 351 – 248 271 276 282 230 357 406 169 97 88 14 158 46 45 56 74
Endoplasmic Reticulum 3 2 0 0 4 9 5 – 4 1 4 1 0 0 1 17 8 5 14 24 10 10 17 18
Endosome 0 1 0 0 0 2 0 – 0 0 0 1 2 0 1 1 0 2 1 6 1 3 1 2
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 2 3 1 0 0 0 2 0 1
Mitochondria 1 18 1 5 4 31 74 – 106 93 156 109 1 3 5 8 9 11 1 39 21 19 17 12
Nucleus 0 2 2 1 1 0 5 – 1 3 5 1 0 0 1 5 2 3 0 0 1 0 0 0
Nuclear Periphery 1 0 0 0 0 0 0 – 1 0 0 0 0 0 1 0 0 0 0 0 0 0 2 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Peroxisomes 2 0 0 0 0 0 1 – 1 0 0 0 1 4 2 0 3 0 0 0 0 0 1 0
SpindlePole 7 1 16 3 22 11 8 – 4 4 4 6 8 2 1 23 15 5 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 0 2 0 1 – 0 4 2 1 1 0 0 1 8 2 3 26 7 10 8 5
Unique Cell Count 150 333 280 234 320 317 460 359 366 429 381 303 394 443 312 198 183 182 408 347 349 439 518
Labelled Cell Count 200 393 378 318 406 395 556 462 448 558 488 409 511 566 393 258 221 182 408 347 349 439 518


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 4.7 6.2 5.8 6.1 5.2 5.3 – 4.5 4.4 4.3 4.8 5.6 5.4 5.6 8.0 8.7 8.0 5.4 5.5 5.8
Std Deviation (1e-4) 0.7 1.0 0.9 0.9 1.3 1.3 1.0 – 0.7 0.9 0.8 0.7 1.0 0.9 1.1 1.3 1.9 1.3 1.1 1.1 1.3
Intensity Change (Log2) – – – -0.1 -0.02 -0.26 -0.23 – -0.45 -0.49 -0.53 -0.38 -0.14 -0.19 -0.15 0.37 0.49 0.38 -0.19 -0.17 -0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.8494 4.0846 3.5958 3.1449 3.393 3.7797 1.2847 2.109 2.0046 1.7719 1.4355 1.8331 0.653 2.0883 1.621 1.6484 1.4064 1.5961
Actin 0.0252 0.0033 0.0306 0.0009 0.0074 0.0106 0.0417 0.0048 0.026 0.006 0.0291 0.012 0.0433 0.0057 0.0026 0.0078 0.0101 0.0099
Bud 0.0007 0.0006 0.0013 0.0019 0.0023 0.0013 0.0026 0.0005 0.0023 0.0042 0.0021 0.0013 0.0013 0.0006 0.0008 0.0012 0.0013 0.0007
Bud Neck 0.0058 0.0083 0.0101 0.0099 0.1854 0.2434 0.0092 0.0059 0.0142 0.0096 0.0473 0.2387 0.0235 0.0045 0.0138 0.0218 0.0354 0.2883
Bud Periphery 0.0081 0.0032 0.0061 0.0238 0.0105 0.0088 0.0118 0.0016 0.0119 0.0202 0.0175 0.0116 0.0081 0.0017 0.0059 0.0144 0.0136 0.0063
Bud Site 0.0408 0.1424 0.0771 0.0128 0.0367 0.0067 0.0481 0.2146 0.0678 0.0181 0.0373 0.0057 0.0627 0.1857 0.0601 0.0251 0.0143 0.0033
Cell Periphery 0.2187 0.2166 0.3154 0.5856 0.1981 0.1779 0.2256 0.2695 0.3772 0.3696 0.5382 0.2578 0.2005 0.2108 0.3936 0.5416 0.4971 0.1944
Cytoplasm 0.3899 0.5168 0.4317 0.3538 0.3775 0.4813 0.427 0.4418 0.4035 0.3678 0.2131 0.3894 0.4436 0.5419 0.4516 0.3818 0.3196 0.4635
Cytoplasmic Foci 0.0107 0.0018 0.006 0.0004 0.013 0.0023 0.0252 0.0031 0.0114 0.0404 0.0097 0.0041 0.0187 0.0016 0.0034 0.0005 0.0073 0.0018
Eisosomes 0.0039 0.0018 0.0025 0.001 0.0028 0.0006 0.003 0.0018 0.0026 0.0006 0.0014 0.0008 0.0041 0.001 0.0012 0.0007 0.0019 0.0003
Endoplasmic Reticulum 0.0054 0.0029 0.0043 0.0024 0.0049 0.003 0.0103 0.0039 0.0057 0.0043 0.0072 0.0018 0.0083 0.0019 0.0026 0.0014 0.0021 0.0016
Endosome 0.0053 0.0003 0.0055 0 0.0013 0.0055 0.0303 0.0011 0.0085 0.0402 0.0198 0.0056 0.0105 0.0002 0.0012 0 0.0135 0.0017
Golgi 0.0019 0.0005 0.0037 0.0002 0.0012 0.0029 0.0177 0.0004 0.0059 0.0134 0.0207 0.0038 0.005 0.0003 0.0018 0.0002 0.0058 0.0008
Lipid Particles 0.0084 0.0015 0.0075 0.0003 0.0032 0.0045 0.0198 0.0013 0.0091 0.0255 0.0072 0.0045 0.0097 0.0005 0.004 0.0002 0.0079 0.0004
Mitochondria 0.0011 0.0001 0.0044 0.0001 0.0027 0.0028 0.0143 0.0001 0.0041 0.006 0.0254 0.0082 0.0125 0.0001 0.008 0 0.0103 0.0001
None 0.2457 0.0964 0.076 0.0037 0.143 0.0396 0.0704 0.0474 0.0338 0.032 0.0132 0.0442 0.1213 0.0414 0.0345 0.0011 0.0193 0.0227
Nuclear Periphery 0.0033 0.0002 0.0031 0.0001 0.0002 0.0015 0.0088 0.0002 0.0015 0.0022 0.0019 0.0006 0.0024 0.0001 0.0002 0.0001 0.011 0.0015
Nucleolus 0.0015 0.0002 0.0006 0.0001 0.002 0.0004 0.001 0.0001 0.0005 0.0013 0.0003 0.0006 0.0017 0.0001 0.0002 0.0001 0.0006 0.0001
Nucleus 0.0039 0.0004 0.001 0.0003 0.0009 0.0007 0.0094 0.0004 0.0015 0.0034 0.0011 0.001 0.0012 0.0003 0.0004 0.0002 0.0016 0.0005
Peroxisomes 0.0062 0.0001 0.0077 0 0.0016 0.0021 0.0124 0.0003 0.0079 0.0137 0.0025 0.0024 0.014 0.0001 0.0117 0 0.0104 0.0003
Punctate Nuclear 0.0091 0.0002 0.0017 0 0.0004 0.001 0.003 0.0002 0.001 0.0046 0.001 0.0034 0.0017 0.0001 0.0002 0 0.0146 0.0005
Vacuole 0.004 0.0023 0.0033 0.0025 0.0048 0.0026 0.0067 0.001 0.0032 0.0149 0.0035 0.002 0.0052 0.0014 0.0024 0.0016 0.0016 0.0012
Vacuole Periphery 0.0004 0 0.0003 0 0.0003 0.0003 0.0017 0 0.0003 0.002 0.0006 0.0004 0.0006 0 0.0001 0 0.0007 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.7293 21.7553 11.2699 14.913 19.6464 16.0623 21.7892 19.7484 14.7682 24.7295
Translational Efficiency 0.6353 0.7058 0.813 0.5596 0.5595 0.76 0.7935 0.5105 0.5851 0.5156

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1959 1059 1583 1566 138 1468 1657 276 2097 2527 3240 1842

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 752.80 844.22 929.46 985.93 836.61 781.32 951.64 985.27 758.32 807.68 940.80 985.83
Standard Deviation 81.07 120.22 146.36 113.60 94.12 112.09 120.52 117.49 84.58 119.66 134.23 114.19
Intensity Change Log 2 — 0.165352 0.304126 0.389219 — -0.098642 0.185861 0.235964 — 0.032428 0.243085 0.310583

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.005298 0.023096 0.024463 0.067417 0.006938 0.017146 0.023643 0.028452 0.005406 0.019639 0.024044 0.061579
Bud Neck 0.179435 0.127769 0.041727 0.078315 0.113147 0.138036 0.071093 0.045574 0.175073 0.133733 0.056745 0.073409
Bud Site 0.052915 0.114813* 0.157463* 0.215187* 0.097705 0.092292 0.130405* 0.153408* 0.055862 0.101730* 0.143625* 0.205930*
Cell Periphery 0.099958 0.037500 0.035271 0.053334 0.070226 0.042467 0.046650 0.026026 0.098002 0.040385 0.041091 0.049243
Cytoplasm 0.521599* 0.453555* 0.366954* 0.315799* 0.481057* 0.461794* 0.369913* 0.276582* 0.518931* 0.458341* 0.368468* 0.309923*
Cytoplasmic Foci 0.017302 0.075544 0.038413 0.014081 0.063870 0.072514 0.028182 0.045783 0.020366 0.073784 0.033180 0.018831
Eisosomes 0.053142 0.027011 0.007196 0.022679 0.022582 0.036513 0.017018 0.012371 0.051131 0.032531 0.012219 0.021135
Endoplasmic Reticulum 0.003420 0.005005 0.042047 0.005852 0.004347 0.004125 0.017356 0.006900 0.003481 0.004494 0.029420 0.006009
Endosome 0.003374 0.012685 0.004229 0.002083 0.008863 0.018259 0.003408 0.007110 0.003736 0.015923 0.003809 0.002836
Golgi 0.008809 0.013239 0.001215 0.002437 0.009177 0.022507 0.000932 0.000600 0.008833 0.018623 0.001070 0.002162
Lipid Particles 0.007865 0.007815 0.005127 0.002578 0.010281 0.013274 0.004563 0.004527 0.008024 0.010986 0.004838 0.002870
Mitochondria 0.004089 0.003373 0.000502 0.001524 0.001278 0.008755 0.000354 0.000771 0.003904 0.006499 0.000426 0.001411
Mitotic Spindle 0.000134 0.000956 0.000631 0.002258 0.000712 0.000671 0.002574 0.001968 0.000172 0.000790 0.001625 0.002214
None 0.001542 0.000310 0.001291 0.000211 0.003362 0.001003 0.000881 0.000813 0.001661 0.000712 0.001081 0.000301
Nuclear Periphery 0.000092 0.000106 0.000281 0.000093 0.000151 0.000150 0.000198 0.000136 0.000095 0.000132 0.000239 0.000099
Nuclear Periphery Foci 0.000329 0.000784 0.005194 0.001433 0.001636 0.000405 0.002421 0.002496 0.000415 0.000563 0.003776 0.001592
Nucleolus 0.000138 0.000144 0.000130 0.000030 0.000549 0.000281 0.000096 0.000089 0.000165 0.000224 0.000113 0.000039
Nucleus 0.011745 0.024467 0.152797* 0.034841 0.031902 0.019678 0.086565 0.021510 0.013071 0.021685 0.118925 0.032844
Peroxisomes 0.006623 0.010027 0.001637 0.001555 0.004832 0.011779 0.000971 0.006198 0.006505 0.011045 0.001296 0.002251
Vacuole 0.021738 0.060787 0.113264 0.177314 0.066924 0.037022 0.192657 0.356045* 0.024712 0.046981 0.153867 0.204094*
Vacuole Periphery 0.000453 0.001014 0.000168 0.000979 0.000458 0.001330 0.000120 0.002643 0.000454 0.001198 0.000144 0.001228

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -9.06 -13.14 -19.67 -11.20 -12.02 -6.51 -10.09 -4.74 -1.75 -0.21 -12.41 -18.08 -20.11 -13.34 -12.33
Bud Neck 6.19 22.32 15.96 8.21 -7.14 -1.40 2.68 4.25 13.99 4.79 6.04 20.34 17.14 13.56 -3.06
Bud Site -11.17 -17.84 -23.73 -11.17 -6.38 0.37 -1.84 -1.86 -3.17 -0.55 -11.88 -19.75 -23.38 -14.56 -8.42
Cell Periphery 14.87 17.47 13.15 -2.68 -4.43 2.36 2.31 4.12 4.77 5.09 15.92 17.26 14.57 -1.01 -1.90
Cytoplasm 7.20 18.33 24.17 14.40 4.64 0.83 5.21 7.87 11.41 5.51 7.98 21.56 26.00 19.70 7.01
Cytoplasmic Foci -15.67 -10.84 0.85 16.09 11.70 -1.10 4.89 2.03 4.29 -3.18 -20.49 -8.56 -0.16 20.21 8.19
Eisosomes 10.98 24.87 15.21 3.43 -10.31 -3.72 1.95 3.27 10.46 2.80 8.99 22.31 16.16 8.72 -6.10
Endoplasmic Reticulum -2.20 -18.97 -4.39 -0.99 17.77 0.24 -9.03 -1.44 -1.83 7.65 -1.80 -21.36 -4.72 -2.23 19.30
Endosome -8.09 -1.67 3.36 9.17 4.09 -3.94 2.87 0.97 5.63 -2.07 -12.20 -0.20 2.38 12.94 2.56
Golgi -4.16 17.87 8.12 9.25 -1.40 -4.79 3.54 3.71 14.68 2.06 -9.29 18.16 9.46 14.72 -1.46
Lipid Particles 0.05 4.40 9.81 8.62 9.84 -0.92 1.97 1.90 5.87 0.02 -3.28 5.62 9.33 10.60 8.43
Mitochondria 0.84 7.17 3.55 2.19 -1.44 -6.17 1.92 0.95 6.65 -0.50 -3.04 7.41 3.87 6.03 -1.60
Mitotic Spindle -1.74 -5.09 -2.52 -1.25 -1.84 0.09 -1.97 -0.75 -0.82 0.65 -2.76 -3.15 -2.71 -1.74 -0.32
None 2.50 0.64 2.73 1.44 6.90 0.80 0.81 0.87 0.51 0.36 1.88 1.12 2.72 3.27 4.06
Nuclear Periphery -0.32 -16.36 -0.87 0.01 9.72 0.03 -1.37 0.16 0.08 3.17 -0.80 -17.63 -1.07 0.42 9.09
Nuclear Periphery Foci -2.36 -12.08 -12.17 -3.70 8.99 4.74 -3.03 -2.70 -7.72 -0.53 -1.74 -15.88 -13.28 -9.34 9.28
Nucleolus -0.49 0.04 10.21 5.57 6.52 0.84 1.35 1.40 3.53 0.63 -1.47 1.74 4.89 5.94 6.10
Nucleus -8.63 -34.05 -15.65 -6.08 26.25 1.91 -7.72 1.55 -1.02 19.08 -8.55 -40.19 -14.67 -8.46 28.81
Peroxisomes -3.19 9.93 9.83 8.74 0.29 -3.76 2.32 -0.07 2.51 -1.59 -5.98 11.13 7.14 12.21 -1.78
Vacuole -13.96 -27.33 -32.72 -23.74 -12.44 3.70 -15.38 -17.15 -20.31 -10.10 -12.85 -41.44 -36.69 -32.30 -10.28
Vacuole Periphery -2.13 6.50 -1.05 0.34 -1.81 -4.89 3.39 -0.85 -0.39 -1.04 -5.16 7.46 -1.35 0.36 -2.09
Standard name
Human Ortholog
Description Serine/threonine protein kinase; inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck; KIN4 has a paralog, FRK1, that arose from the whole genome duplication
Localization
Cell Percentages cell periphery (34%), cytoplasm (5%), mixed (48%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Kin4

Kin4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Kin4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available