Standard name
Description Serine/threonine protein kinase; inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck; KIN4 has a paralog, FRK1, that arose from the whole genome duplication

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.19 0.05 0.11 0.07 0.14 0.10 0.06 0.06 0 0 0 0.10 0 0 0.13 0.15 0.10 0.09 0.06 0.06 0.07 0.08 0.05
Bud 0.09 0.12 0 0 0 0 0 0.06 0.06 0.07 0 0.09 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0.10 0.11 0.11 0.07 0.09
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.42 0.15 0.40 0.46 0.23 0.24 0.14 0.14 0.10 0.16 0.15 0.34 0.31 0.30 0.36 0.38 0.43 0.56 0.16 0.55 0.51 0.59 0.61
Cytoplasm 0.51 0.77 0.69 0.74 0.72 0.68 0.76 0.69 0.74 0.64 0.74 0.76 0.91 0.92 0.54 0.49 0.48 0.08 0.39 0.14 0.13 0.13 0.14
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.08 0.06 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 0 0 0.10 0.16 0.30 0.25 0.36 0.29 0 0 0 0 0.05 0.06 0 0.10 0.06 0.06 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.05 0 0.06 0 0.07 0 0 0 0 0 0 0 0 0 0.07 0.08 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 28 18 30 16 45 33 26 20 10 13 11 31 9 7 42 30 19 15 24 20 25 33 26
Bud 14 41 11 10 13 11 14 21 22 28 16 27 12 8 10 3 3 2 13 6 7 2 9
Bud Neck 0 1 7 0 6 4 0 2 2 3 1 0 0 0 0 1 0 23 39 36 38 31 44
Bud Site 4 3 4 3 4 4 5 2 2 0 1 6 0 1 3 3 3
Cell Periphery 63 51 112 108 74 75 66 52 36 67 58 102 124 132 112 76 79 101 65 190 177 259 315
Cytoplasm 77 255 194 172 231 215 351 248 271 276 282 230 357 406 169 97 88 14 158 46 45 56 74
Endoplasmic Reticulum 3 2 0 0 4 9 5 4 1 4 1 0 0 1 17 8 5 14 24 10 10 17 18
Endosome 0 1 0 0 0 2 0 0 0 0 1 2 0 1 1 0 2 1 6 1 3 1 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 3 1 0 0 0 2 0 1
Mitochondria 1 18 1 5 4 31 74 106 93 156 109 1 3 5 8 9 11 1 39 21 19 17 12
Nucleus 0 2 2 1 1 0 5 1 3 5 1 0 0 1 5 2 3 0 0 1 0 0 0
Nuclear Periphery 1 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 2 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Peroxisomes 2 0 0 0 0 0 1 1 0 0 0 1 4 2 0 3 0 0 0 0 0 1 0
SpindlePole 7 1 16 3 22 11 8 4 4 4 6 8 2 1 23 15 5 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 0 2 0 1 0 4 2 1 1 0 0 1 8 2 3 26 7 10 8 5
Unique Cell Count 150 333 280 234 320 317 460 359 366 429 381 303 394 443 312 198 183 182 408 347 349 439 518
Labelled Cell Count 200 393 378 318 406 395 556 462 448 558 488 409 511 566 393 258 221 182 408 347 349 439 518

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Cell Periphery

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 4.7 6.2 5.8 6.1 5.2 5.3 4.5 4.4 4.3 4.8 5.6 5.4 5.6 8.0 8.7 8.0 5.4 5.5 5.8
Std Deviation (1e-4) 0.7 1.0 0.9 0.9 1.3 1.3 1.0 0.7 0.9 0.8 0.7 1.0 0.9 1.1 1.3 1.9 1.3 1.1 1.1 1.3
Intensity Change (Log2) -0.1 -0.0 -0.3 -0.2 -0.5 -0.5 -0.5 -0.4 -0.1 -0.2 -0.1 0.4 0.5 0.4 -0.2 -0.2 -0.1

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole