ORF
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.96 0.97 1.0 0.93 0.92 0.88 0.85 0.74 0.69 0.65 0.62 1.0 0.99 0.99 0.98 0.98 0.99 0.91 0.92 0.9 0.91 0.94 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.05 0.1 0.13 0.14 0.4 0.45 0.51 0.49 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.08 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 2 3 1 2 0 1
Bud 0 2 0 0 0 1 0 1 1 3 2 1 0 0 0 0 0 0 1 2 0 0 3 4
Bud Neck 0 1 2 0 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 1 0 1 0 2
Bud Site 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cell Periphery 1 0 2 0 0 3 0 2 0 1 0 0 1 2 0 1 9 4 0 0 0 0 0 0
Cytoplasm 267 451 100 118 78 119 141 189 70 68 48 39 290 394 624 231 274 267 256 445 109 230 312 317
Endoplasmic Reticulum 2 3 1 0 0 0 0 0 0 0 0 1 0 2 2 12 14 13 0 0 1 2 1 4
Endosome 0 0 0 0 0 0 0 1 0 0 0 0 0 2 1 1 0 0 2 4 0 0 1 4
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2 1 1 0 1 3
Mitochondria 0 13 0 0 4 13 21 32 38 44 38 31 0 0 0 4 5 3 2 11 3 1 1 1
Nucleus 1 2 2 2 5 3 4 1 0 2 1 2 0 0 1 1 3 1 1 1 0 0 1 1
Nuclear Periphery 1 0 0 0 0 0 2 0 1 0 0 0 0 0 0 1 0 0 0 1 0 2 1 1
Nucleolus 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 3 2 0 1 1 1
Vac/Vac Membrane 2 3 0 0 0 0 6 18 4 2 3 4 3 3 3 3 8 6 5 5 0 1 0 3
Unique Cell Count 271 468 103 118 84 129 161 223 94 98 74 63 291 396 629 236 281 270 281 487 122 252 332 359
Labelled Cell Count 274 477 107 120 88 139 178 244 115 120 94 79 294 403 631 255 315 295 281 487 122 252 332 359


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.6 4.7 6.5 5.7 4.7 5.3 5.1 5.5 4.5 4.6 4.8 5.9 6.4 6.7 6.0 5.6 6.8 6.8 6.9 7.3 7.9
Std Deviation (1e-4) 1.0 0.8 1.8 0.7 0.9 1.1 0.9 1.1 1.2 1.2 1.9 5.3 1.1 1.1 1.0 1.3 1.7 1.5 1.8 1.4 1.8
Intensity Change (Log2) -0.2 -0.46 -0.3 -0.34 -0.25 -0.52 -0.49 -0.45 -0.15 -0.03 0.04 -0.11 -0.21 0.06 0.06 0.08 0.17 0.29


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yor238w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available