Standard name
Human Ortholog
Description Catalytic subunit of the histone acetyltransferase complex (NuA4); acetylates four conserved internal lysines of histone H4 N-terminal tail and can acetylate histone H2A; master regulator of cellular acetylation balance; required for cell cycle progression and transcriptional silencing at the rDNA locus and regulation of autophagy; human ortholog TIP60/KAT5 is implicated in cancer and other diseases, functionally complements lethality of the esa1 null mutation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.05 0.06 0.09 0.12 0.07 0.06 0.06 0.06 0.08 0.07 0.05 0 0 0 0.11 0.14 0.11 0 0 0.05 0 0 0
Bud 0 0 0 0.07 0 0 0 0 0.06 0.05 0.09 0.05 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.07 0.09 0.06 0.05 0.07 0.09 0.06 0.09 0.1 0.13 0.06 0.05 0.09 0.32 0.35 0.37 0 0 0 0 0 0
Cytoplasm 0.41 0.57 0.34 0.44 0.46 0.22 0.19 0.22 0.28 0.24 0.38 0.33 0.5 0.66 0.76 0.46 0.49 0.38 0.09 0.16 0.05 0.21 0.29 0.31
Endoplasmic Reticulum 0.48 0.41 0.6 0.33 0.31 0.62 0.56 0.53 0.4 0.38 0.19 0.3 0.44 0.33 0.22 0 0 0 0.73 0.6 0.78 0.54 0.46 0.45
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0.11 0 0 0 0 0 0
Mitochondria 0.15 0 0 0.27 0.19 0.26 0.31 0.24 0.25 0.33 0.37 0.34 0 0 0 0 0 0 0.05 0 0 0.07 0.05 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0 0 0 0.06 0 0 0.07 0.09 0.08 0.09 0.06 0 0 0 0.12 0.06 0.14 0 0.07 0 0.07 0.06 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 2 12 12 20 16 22 18 18 31 18 20 6 5 1 19 20 15 8 3 11 3 12 17
Bud 5 1 4 9 7 2 14 10 18 21 24 20 1 2 1 5 6 1 10 3 4 2 7 19
Bud Neck 0 0 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 2 0 2 2 3 4
Bud Site 0 0 0 0 0 0 1 0 0 1 2 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 0 15 12 11 11 26 28 19 35 27 47 16 17 35 54 52 49 2 0 5 3 9 7
Cytoplasm 94 25 75 58 79 52 68 65 87 92 99 124 133 210 301 78 72 51 23 12 13 34 109 127
Endoplasmic Reticulum 110 18 131 44 53 146 198 158 124 145 50 111 117 106 88 4 4 4 196 48 196 89 172 186
Endosome 1 0 1 2 6 0 5 0 3 0 1 2 3 0 0 1 1 0 0 0 1 1 5 2
Golgi 1 0 1 0 5 0 0 0 0 0 0 0 0 0 0 8 12 14 2 0 1 0 0 1
Mitochondria 34 1 8 35 32 62 110 72 77 128 97 129 0 0 0 3 5 5 14 3 8 11 19 14
Nucleus 0 0 1 0 0 0 2 0 1 0 2 1 0 4 0 1 0 1 0 0 0 2 1 1
Nuclear Periphery 0 0 1 2 1 2 8 6 3 6 0 9 0 0 0 0 0 0 0 2 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 1 1 0 0 1 1 1 0 2 0 0 2 0 0 0 0 0 1 0 0
Vac/Vac Membrane 16 1 7 4 10 6 10 21 29 29 24 23 11 8 15 21 9 18 4 5 3 11 21 21
Unique Cell Count 229 44 218 132 172 237 354 299 308 384 262 374 265 319 394 170 147 133 271 80 251 166 373 410
Labelled Cell Count 269 48 258 179 226 299 464 378 381 489 346 487 289 352 442 196 181 158 271 80 251 166 373 410


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.0 7.9 6.6 6.7 8.2 8.8 8.3 8.0 7.9 7.5 7.1 7.0 8.5 7.5 6.8 18.3 19.4 20.6 8.3 8.2 8.1
Std Deviation (1e-4) 1.6 2.5 1.6 2.1 3.4 2.5 2.5 2.3 2.4 2.5 2.8 2.5 2.6 1.8 1.6 9.4 9.5 11.1 2.0 1.7 1.9
Intensity Change (Log2) – – – 0.02 0.3 0.41 0.34 0.28 0.26 0.18 0.11 0.09 0.37 0.19 0.04 1.47 1.55 1.64 0.33 0.3 0.29


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.3 2.2 0.6 0.3 0.2 0.2 1.2 0.6 -0.1 -1.9 -2.6 0 2.0 2.7 2.0
Bud 0 0 0 1.4 1.0 2.3 2.1 3.4 2.1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.8 -0.2 -1.0 0.2 1.0 -0.3 1.0 1.3 2.2 -0.4 -0.7 0.9 6.4 6.9 7.1
Cytoplasm 1.8 2.3 -3.0 -4.1 -3.2 -1.5 -2.8 0.8 -0.3 3.5 7.2 10.2 2.3 2.8 0.7
Endoplasmic Reticulum -4.9 -5.8 0.3 -1.0 -1.6 -4.5 -5.3 -9.2 -7.3 -3.5 -6.2 -9.3 -11.8 -11.1 -10.7
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 3.9 4.5
Mitochondria 6.3 4.8 6.6 7.9 6.3 6.5 8.4 8.8 8.6 0 0 0 0 -0.1 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.3 -0.4 -0.3 1.9 2.8 2.2 2.6 1.6 0.5 -0.5 0.4 3.5 1.3 3.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.2277 6.5775 4.7226 4.9375 3.705 7.0321 5.204 6.1648 4.3377 3.5555 2.7209 5.6702 4.8794 5.7095 3.2798 2.478 0.6044 5.0466
Actin 0.003 0.0043 0.006 0.0088 0.0088 0.0067 0.038 0.0063 0.0088 0.0224 0.0221 0.001 0.0235 0.002 0.0231 0.0513 0.0173 0.003
Bud 0.0002 0.0064 0.0014 0.0074 0.0029 0.0002 0.001 0.002 0.0018 0.0006 0.0049 0.0002 0.0004 0.0002 0.0004 0.0051 0.0017 0.0002
Bud Neck 0.0002 0.0006 0.0007 0.0024 0.0022 0.0021 0.0012 0.0007 0.0012 0.0012 0.0012 0.0093 0.0028 0.0002 0.0007 0.0035 0.0007 0.0033
Bud Periphery 0.0016 0.0161 0.0056 0.0088 0.0139 0.0008 0.004 0.0037 0.0083 0.001 0.0545 0.0008 0.0013 0.0009 0.0009 0.0089 0.0145 0.001
Bud Site 0.0011 0.0132 0.0036 0.006 0.0007 0.0026 0.0027 0.0207 0.013 0.0014 0.0023 0.0002 0.002 0.0012 0.0038 0.0043 0.0006 0.0002
Cell Periphery 0.0028 0.0133 0.0059 0.0093 0.0077 0.0025 0.0067 0.0075 0.005 0.0018 0.0026 0.0014 0.0023 0.0083 0.0033 0.005 0.0056 0.0019
Cytoplasm 0.1666 0.1775 0.2324 0.2117 0.3273 0.1431 0.1187 0.2565 0.2402 0.2269 0.2661 0.2328 0.09 0.161 0.1863 0.261 0.4732 0.1758
Cytoplasmic Foci 0.0136 0.0033 0.011 0.0083 0.0137 0.0065 0.013 0.0042 0.0097 0.0135 0.0117 0.0068 0.013 0.0029 0.0126 0.0257 0.0127 0.0071
Eisosomes 0.0002 0.0004 0.0004 0.0007 0.0008 0.0002 0.0019 0.0002 0.0002 0.0003 0.0003 0 0.0003 0.0001 0.0002 0.0007 0.0004 0
Endoplasmic Reticulum 0.6756 0.6554 0.5961 0.551 0.4791 0.7622 0.6279 0.602 0.583 0.6122 0.4061 0.689 0.7159 0.7772 0.6729 0.4552 0.2791 0.7453
Endosome 0.0183 0.005 0.0092 0.02 0.007 0.0119 0.059 0.0109 0.02 0.0227 0.0407 0.0097 0.0345 0.0051 0.0186 0.0237 0.0445 0.0147
Golgi 0.0035 0.0011 0.0024 0.0082 0.0029 0.004 0.0208 0.0013 0.0066 0.016 0.011 0.0008 0.0086 0.003 0.0036 0.0072 0.0067 0.0028
Lipid Particles 0.0271 0.0061 0.012 0.0107 0.0068 0.0054 0.03 0.0103 0.0129 0.0125 0.0437 0.0058 0.0192 0.012 0.0095 0.0116 0.0596 0.0098
Mitochondria 0.0039 0.0038 0.0009 0.0098 0.0028 0.0017 0.0254 0.0007 0.0083 0.006 0.0344 0.0006 0.0135 0.0046 0.002 0.0033 0.0073 0.0007
None 0.0618 0.0706 0.0893 0.0883 0.0862 0.0367 0.0066 0.0411 0.0402 0.0137 0.0161 0.0195 0.0199 0.0023 0.0329 0.0841 0.0376 0.0174
Nuclear Periphery 0.0107 0.0097 0.0103 0.0109 0.0051 0.0072 0.0166 0.0149 0.0137 0.0225 0.0154 0.0089 0.0258 0.0109 0.011 0.0154 0.0146 0.0073
Nucleolus 0.0002 0.0006 0.0002 0.0013 0.0005 0.0001 0.0005 0.0002 0.0003 0.0002 0.002 0.0001 0.0007 0 0.0002 0.0017 0.0005 0.0001
Nucleus 0.0015 0.0031 0.0021 0.0077 0.0026 0.0014 0.0034 0.0032 0.0061 0.0061 0.0254 0.0024 0.0036 0.0012 0.0019 0.0043 0.0023 0.0015
Peroxisomes 0.0029 0.0002 0.0005 0.0003 0.0164 0.0002 0.0056 0.0001 0.0058 0.0076 0.0104 0.0031 0.0103 0.0001 0.0021 0.0036 0.0071 0.0012
Punctate Nuclear 0.0013 0.0017 0.0016 0.016 0.0046 0.0005 0.0038 0.0012 0.0025 0.005 0.0091 0.0009 0.0041 0.0003 0.0066 0.0141 0.0076 0.001
Vacuole 0.0037 0.0071 0.0076 0.0099 0.0074 0.0036 0.0104 0.0098 0.0109 0.0052 0.0133 0.0061 0.006 0.006 0.0065 0.0089 0.0051 0.0052
Vacuole Periphery 0.0005 0.0006 0.0008 0.0026 0.0005 0.0005 0.0028 0.0023 0.0014 0.001 0.0069 0.0006 0.0023 0.0005 0.0008 0.0014 0.0014 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.844 13.2696 10.3937 16.7812 7.4845 9.8905 18.2619 16.6018 16.019 19.2911
Translational Efficiency 1.0241 1.0471 1.1689 0.9158 2.0703 1.6649 0.8871 0.8508 0.999 0.9484

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Catalytic subunit of the histone acetyltransferase complex (NuA4); acetylates four conserved internal lysines of histone H4 N-terminal tail and can acetylate histone H2A; master regulator of cellular acetylation balance; required for cell cycle progression and transcriptional silencing at the rDNA locus and regulation of autophagy; human ortholog TIP60/KAT5 is implicated in cancer and other diseases, functionally complements lethality of the esa1 null mutation
Localization
Cell Percentages ER (85%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Esa1

Esa1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Esa1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available