Standard name | |
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Description | Catalytic subunit of the histone acetyltransferase complex (NuA4); acetylates four conserved internal lysines of histone H4 N-terminal tail and can acetylate histone H2A; master regulator of cellular acetylation balance; required for cell cycle progression and transcriptional silencing at the rDNA locus and regulation of autophagy; human ortholog TIP60/KAT5 is implicated in cancer and other diseases, functionally complements lethality of the esa1 null mutation |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 5 | 2 | 12 | 12 | 20 | 16 | 22 | 18 | 18 | 31 | 18 | 20 | 6 | 5 | 1 | 19 | 20 | 15 | 8 | 3 | 11 | 3 | 12 | 17 |
Bud | 5 | 1 | 4 | 9 | 7 | 2 | 14 | 10 | 18 | 21 | 24 | 20 | 1 | 2 | 1 | 5 | 6 | 1 | 10 | 3 | 4 | 2 | 7 | 19 |
Bud Neck | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 2 | 2 | 3 | 4 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 0 | 15 | 12 | 11 | 11 | 26 | 28 | 19 | 35 | 27 | 47 | 16 | 17 | 35 | 54 | 52 | 49 | 2 | 0 | 5 | 3 | 9 | 7 |
Cytoplasm | 94 | 25 | 75 | 58 | 79 | 52 | 68 | 65 | 87 | 92 | 99 | 124 | 133 | 210 | 301 | 78 | 72 | 51 | 23 | 12 | 13 | 34 | 109 | 127 |
Endoplasmic Reticulum | 110 | 18 | 131 | 44 | 53 | 146 | 198 | 158 | 124 | 145 | 50 | 111 | 117 | 106 | 88 | 4 | 4 | 4 | 196 | 48 | 196 | 89 | 172 | 186 |
Endosome | 1 | 0 | 1 | 2 | 6 | 0 | 5 | 0 | 3 | 0 | 1 | 2 | 3 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 5 | 2 |
Golgi | 1 | 0 | 1 | 0 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 12 | 14 | 2 | 0 | 1 | 0 | 0 | 1 |
Mitochondria | 34 | 1 | 8 | 35 | 32 | 62 | 110 | 72 | 77 | 128 | 97 | 129 | 0 | 0 | 0 | 3 | 5 | 5 | 14 | 3 | 8 | 11 | 19 | 14 |
Nucleus | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 2 | 1 | 0 | 4 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 1 |
Nuclear Periphery | 0 | 0 | 1 | 2 | 1 | 2 | 8 | 6 | 3 | 6 | 0 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 2 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Vac/Vac Membrane | 16 | 1 | 7 | 4 | 10 | 6 | 10 | 21 | 29 | 29 | 24 | 23 | 11 | 8 | 15 | 21 | 9 | 18 | 4 | 5 | 3 | 11 | 21 | 21 |
Unique Cell Count | 229 | 44 | 218 | 132 | 172 | 237 | 354 | 299 | 308 | 384 | 262 | 374 | 265 | 319 | 394 | 170 | 147 | 133 | 271 | 80 | 251 | 166 | 373 | 410 |
Labelled Cell Count | 269 | 48 | 258 | 179 | 226 | 299 | 464 | 378 | 381 | 489 | 346 | 487 | 289 | 352 | 442 | 196 | 181 | 158 | 271 | 80 | 251 | 166 | 373 | 410 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.0 | 7.9 | 6.6 | 6.7 | 8.2 | 8.8 | 8.3 | 8.0 | 7.9 | 7.5 | 7.1 | 7.0 | 8.5 | 7.5 | 6.8 | 18.3 | 19.4 | 20.6 | 8.3 | 8.2 | 8.1 |
Std Deviation (1e-4) | 1.6 | 2.5 | 1.6 | 2.1 | 3.4 | 2.5 | 2.5 | 2.3 | 2.4 | 2.5 | 2.8 | 2.5 | 2.6 | 1.8 | 1.6 | 9.4 | 9.5 | 11.1 | 2.0 | 1.7 | 1.9 |
Intensity Change (Log2) | – | – | – | 0.02 | 0.3 | 0.41 | 0.34 | 0.28 | 0.26 | 0.18 | 0.11 | 0.09 | 0.37 | 0.19 | 0.04 | 1.47 | 1.55 | 1.64 | 0.33 | 0.3 | 0.29 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.3 | 2.2 | 0.6 | 0.3 | 0.2 | 0.2 | 1.2 | 0.6 | -0.1 | -1.9 | -2.6 | 0 | 2.0 | 2.7 | 2.0 |
Bud | 0 | 0 | 0 | 1.4 | 1.0 | 2.3 | 2.1 | 3.4 | 2.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.8 | -0.2 | -1.0 | 0.2 | 1.0 | -0.3 | 1.0 | 1.3 | 2.2 | -0.4 | -0.7 | 0.9 | 6.4 | 6.9 | 7.1 |
Cytoplasm | 1.8 | 2.3 | -3.0 | -4.1 | -3.2 | -1.5 | -2.8 | 0.8 | -0.3 | 3.5 | 7.2 | 10.2 | 2.3 | 2.8 | 0.7 |
Endoplasmic Reticulum | -4.9 | -5.8 | 0.3 | -1.0 | -1.6 | -4.5 | -5.3 | -9.2 | -7.3 | -3.5 | -6.2 | -9.3 | -11.8 | -11.1 | -10.7 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.9 | 4.5 |
Mitochondria | 6.3 | 4.8 | 6.6 | 7.9 | 6.3 | 6.5 | 8.4 | 8.8 | 8.6 | 0 | 0 | 0 | 0 | -0.1 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 1.3 | -0.4 | -0.3 | 1.9 | 2.8 | 2.2 | 2.6 | 1.6 | 0.5 | -0.5 | 0.4 | 3.5 | 1.3 | 3.6 |