Standard name
Description Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.06 0.08 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.08 0 0 0.06 0.11 0.08
Bud 0 0 0 0.06 0 0 0.05 0 0.08 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.52 0.40 0.76 0.43 0.69 0.45 0.25 0.20 0.09 0.09 0.11 0.09 0.15 0.14 0.22 0.16 0.12 0.19 0.21 0.16 0.18 0.08 0.11 0.18
Endoplasmic Reticulum 0.14 0.32 0.12 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0.17 0.16 0.13 0.05 0.05 0.07
Endosome 0 0.06 0 0.08 0.07 0.15 0.09 0.08 0 0 0 0 0.22 0.39 0.45 0.51 0.52 0.53 0.13 0.23 0.08 0.24 0.20 0.16
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0
Mitochondria 0.47 0.24 0 0.17 0.08 0.36 0.60 0.67 0.84 0.84 0.89 0.83 0.18 0.07 0.05 0.12 0.13 0.12 0.25 0.24 0.40 0.26 0.25 0.29
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0.18 0.21 0 0 0.07 0 0 0 0.12 0.09 0
SpindlePole 0 0 0.08 0.27 0.07 0.10 0.12 0.13 0.10 0.07 0.06 0.05 0.44 0.44 0.34 0.25 0.22 0.17 0 0 0.05 0.06 0.06 0.05
Vac/Vac Membrane 0 0.06 0 0 0 0.08 0.06 0.06 0 0.07 0 0.08 0 0 0 0.16 0.16 0.12 0.05 0.06 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 5 2 7 6 0 0 1 2 1 0 0 11 3 1 6 6 5 13 2 3 12 67 53
Bud 2 3 0 11 6 4 23 17 29 14 19 9 8 15 7 1 7 1 0 0 0 2 10 14
Bud Neck 1 0 0 3 11 1 1 2 0 1 0 0 2 6 5 0 1 0 1 0 1 3 12 10
Bud Site 0 0 0 0 1 1 5 2 5 5 5 6 0 0 0 0 0 1
Cell Periphery 0 0 1 2 1 0 0 0 1 1 0 0 1 1 1 1 3 0 1 0 1 0 3 5
Cytoplasm 77 36 19 79 216 125 108 80 34 32 32 31 33 78 121 25 25 34 37 18 16 15 68 123
Endoplasmic Reticulum 21 29 3 7 13 0 3 1 2 5 2 10 6 0 1 5 12 12 29 18 12 9 33 45
Endosome 1 5 0 15 23 41 41 31 12 5 1 11 49 214 242 82 104 95 23 26 6 44 130 107
Golgi 0 1 0 1 1 0 0 0 0 0 0 0 0 1 0 1 1 5 0 6 0 5 14 10
Mitochondria 70 22 0 31 26 99 261 275 303 310 257 276 40 41 27 19 26 22 44 27 36 49 163 196
Nucleus 0 1 0 1 3 4 6 8 10 9 9 10 0 2 4 0 2 0 0 0 1 0 0 0
Nuclear Periphery 3 1 0 0 0 0 2 0 5 3 3 8 1 0 0 1 0 1 1 1 0 2 3 3
Nucleolus 0 2 0 0 1 2 4 2 11 2 6 2 9 11 11 2 3 7 0 0 0 0 4 2
Peroxisomes 0 0 0 3 1 8 6 7 2 1 1 2 30 100 113 1 9 13 4 2 0 23 57 29
SpindlePole 2 1 2 49 22 27 53 52 37 27 18 17 99 242 184 40 44 30 5 2 4 10 41 35
Vac/Vac Membrane 0 5 0 3 14 21 26 24 15 25 9 25 3 24 23 25 32 22 7 7 1 4 14 17
Unique Cell Count 148 90 25 184 314 276 433 410 362 367 290 331 226 554 539 160 200 180 176 115 91 189 644 677
Labelled Cell Count 179 111 27 212 345 333 539 502 468 441 362 407 292 738 740 209 275 248 176 115 91 189 644 677

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Lipid Particles

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.4 4.9 5.0 5.1 5.2 4.4 4.2 4.8 4.1 4.2 3.8 4.3 5.8 6.0 5.7 6.4 6.8 6.6 5.2 5.6 5.6
Std Deviation (1e-4) 0.4 0.8 0.5 1.4 1.5 1.3 1.5 1.5 1.0 1.0 0.9 0.8 1.4 1.5 1.7 1.3 1.2 1.3 1.5 1.9 1.7
Intensity Change (Log2) 0.0 0.0 -0.2 -0.3 -0.1 -0.3 -0.3 -0.4 -0.2 0.2 0.2 0.2 0.4 0.4 0.4 0.0 0.2 0.1

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole