Standard name | |
---|---|
Human Ortholog | |
Description | Mannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 8 | 8 | 1 | 2 | 4 | 4 | 15 | – | 2 | 11 | 14 | 8 | 10 | 32 | 26 | 8 | 0 | 2 | 18 | 9 | 7 | 24 | 35 | 30 |
Bud | 3 | 3 | 0 | 3 | 0 | 12 | 9 | – | 7 | 14 | 30 | 39 | 3 | 15 | 5 | 2 | 0 | 0 | 9 | 6 | 5 | 7 | 12 | 8 |
Bud Neck | 0 | 1 | 4 | 2 | 1 | 0 | 2 | – | 0 | 4 | 3 | 3 | 0 | 1 | 0 | 0 | 0 | 1 | 13 | 15 | 12 | 13 | 24 | 18 |
Bud Site | 0 | 2 | 0 | 0 | 0 | 0 | 1 | – | 1 | 0 | 2 | 0 | 0 | 3 | 0 | 1 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 1 | 7 | 2 | 4 | 14 | – | 2 | 9 | 8 | 10 | 10 | 18 | 22 | 6 | 0 | 0 | 1 | 2 | 4 | 5 | 11 | 12 |
Cytoplasm | 182 | 87 | 136 | 199 | 249 | 224 | 359 | – | 184 | 267 | 418 | 537 | 87 | 233 | 234 | 198 | 157 | 99 | 32 | 21 | 15 | 30 | 55 | 70 |
Endoplasmic Reticulum | 26 | 21 | 5 | 1 | 1 | 6 | 11 | – | 9 | 2 | 1 | 2 | 14 | 10 | 11 | 16 | 7 | 8 | 31 | 4 | 10 | 23 | 44 | 48 |
Endosome | 21 | 0 | 0 | 1 | 3 | 1 | 3 | – | 0 | 1 | 1 | 2 | 1 | 15 | 10 | 9 | 0 | 2 | 15 | 0 | 3 | 3 | 2 | 3 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 2 | 1 | 2 |
Mitochondria | 3 | 2 | 0 | 0 | 0 | 3 | 6 | – | 9 | 10 | 25 | 25 | 0 | 1 | 0 | 6 | 0 | 3 | 15 | 6 | 10 | 11 | 8 | 10 |
Nucleus | 3 | 1 | 1 | 1 | 0 | 3 | 3 | – | 3 | 2 | 4 | 6 | 0 | 1 | 0 | 12 | 6 | 9 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 1 | 1 | 10 | 12 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 2 | 0 | 2 | 2 | 2 | 1 | 5 | – | 0 | 5 | 5 | 4 | 2 | 16 | 15 | 8 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 2 |
Vac/Vac Membrane | 69 | 7 | 11 | 10 | 36 | 6 | 27 | – | 3 | 7 | 12 | 20 | 1 | 3 | 5 | 93 | 32 | 40 | 207 | 77 | 120 | 97 | 125 | 100 |
Unique Cell Count | 294 | 125 | 156 | 215 | 272 | 247 | 410 | 211 | 294 | 465 | 590 | 120 | 304 | 308 | 307 | 181 | 145 | 355 | 147 | 195 | 223 | 327 | 314 | |
Labelled Cell Count | 321 | 133 | 161 | 228 | 298 | 264 | 456 | 220 | 334 | 524 | 661 | 130 | 360 | 340 | 361 | 202 | 167 | 355 | 147 | 195 | 223 | 327 | 314 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.5 | 5.5 | 6.1 | 6.2 | 7.3 | 5.4 | 6.9 | – | 5.1 | 5.5 | 5.2 | 4.9 | 6.8 | 6.3 | 6.5 | 6.3 | 6.5 | 6.5 | 5.5 | 5.7 | 5.6 |
Std Deviation (1e-4) | 1.2 | 1.3 | 1.4 | 2.2 | 1.8 | 1.3 | 1.7 | – | 1.1 | 1.6 | 1.5 | 1.1 | 1.6 | 1.7 | 2.1 | 1.3 | 1.6 | 1.6 | 1.3 | 1.7 | 1.7 |
Intensity Change (Log2) | – | – | – | 0.03 | 0.26 | -0.18 | 0.17 | – | -0.25 | -0.14 | -0.22 | -0.33 | 0.15 | 0.06 | 0.08 | 0.04 | 0.1 | 0.09 | -0.15 | -0.1 | -0.13 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 3.9 | 3.4 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 3.3 | 0 | 0 | 2.8 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2.7 | 3.0 | 0 | 0 | 0 |
Cytoplasm | 1.7 | 1.4 | 1.1 | 0.1 | – | 0 | 1.2 | 0.9 | 1.4 | -3.1 | -2.7 | -2.8 | -5.1 | -0.1 | -4.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0.5 | 0 | 0 | 0 | 2.8 | 0 | 0.2 | 1.0 | 0.3 | 1.0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2.8 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 0 | 0 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2.1 | 1.9 | 0 | 0 | 0 |
Vacuole | -1.0 | 2.0 | -2.2 | -0.2 | – | -2.8 | -2.4 | -2.6 | -2.0 | -2.5 | 0 | -3.0 | 5.7 | 2.9 | 4.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.6323 | 0.369 | 0.2292 | -0.0188 | -0.2333 | 0.1224 | 1.9222 | 2.1634 | 2.4998 | 2.4874 | 1.8249 | 2.2861 | 2.2226 | 3.4744 | 3.2069 | 3.1371 | 2.991 | 3.017 |
Actin | 0.0138 | 0.0015 | 0.0083 | 0.0013 | 0.0005 | 0.004 | 0.0302 | 0.0038 | 0.0143 | 0 | 0.0027 | 0.002 | 0.0162 | 0.0006 | 0.0171 | 0 | 0.0211 | 0.0004 |
Bud | 0.0013 | 0.0015 | 0.002 | 0.0009 | 0.0008 | 0.0006 | 0.0011 | 0.0021 | 0.0046 | 0.0003 | 0.0003 | 0.0007 | 0.0014 | 0.0014 | 0.0008 | 0.0005 | 0.0019 | 0.0025 |
Bud Neck | 0.002 | 0.0023 | 0.0029 | 0.0022 | 0.0015 | 0.0066 | 0.0041 | 0.0075 | 0.004 | 0.0017 | 0.0031 | 0.0145 | 0.0016 | 0.0022 | 0.0068 | 0.0011 | 0.0018 | 0.0072 |
Bud Periphery | 0.0019 | 0.0028 | 0.0055 | 0.0017 | 0.0008 | 0.0016 | 0.0025 | 0.0034 | 0.0061 | 0.0007 | 0.0004 | 0.002 | 0.0044 | 0.0031 | 0.0019 | 0.0007 | 0.0077 | 0.0043 |
Bud Site | 0.0054 | 0.0054 | 0.003 | 0.0012 | 0.0065 | 0.0012 | 0.0097 | 0.02 | 0.0231 | 0.0014 | 0.0017 | 0.0021 | 0.004 | 0.0114 | 0.0048 | 0.0012 | 0.0029 | 0.0021 |
Cell Periphery | 0.0025 | 0.0027 | 0.0017 | 0.0021 | 0.001 | 0.003 | 0.0055 | 0.0054 | 0.0026 | 0.0036 | 0.0009 | 0.0053 | 0.0026 | 0.004 | 0.003 | 0.0007 | 0.0017 | 0.0033 |
Cytoplasm | 0.1183 | 0.1361 | 0.0723 | 0.0431 | 0.0467 | 0.0826 | 0.1886 | 0.1903 | 0.1465 | 0.0551 | 0.0679 | 0.1471 | 0.1179 | 0.1652 | 0.115 | 0.0641 | 0.027 | 0.1221 |
Cytoplasmic Foci | 0.0838 | 0.054 | 0.0639 | 0.0704 | 0.0478 | 0.0418 | 0.0905 | 0.0445 | 0.0697 | 0.0125 | 0.0502 | 0.0391 | 0.075 | 0.0329 | 0.054 | 0.0547 | 0.0271 | 0.0334 |
Eisosomes | 0.0007 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0167 | 0.0108 | 0.0164 | 0.0068 | 0.0032 | 0.0091 | 0.029 | 0.0172 | 0.0154 | 0.006 | 0.009 | 0.0138 | 0.0146 | 0.0201 | 0.01 | 0.0039 | 0.0067 | 0.0095 |
Endosome | 0.3297 | 0.2619 | 0.3727 | 0.3363 | 0.3452 | 0.2731 | 0.2562 | 0.1893 | 0.3057 | 0.2769 | 0.4395 | 0.1622 | 0.2669 | 0.1668 | 0.2997 | 0.3804 | 0.3571 | 0.2022 |
Golgi | 0.0186 | 0.0063 | 0.0134 | 0.0163 | 0.0231 | 0.0107 | 0.0272 | 0.0146 | 0.0224 | 0.01 | 0.0138 | 0.0105 | 0.0133 | 0.0063 | 0.0117 | 0.0105 | 0.0122 | 0.0068 |
Lipid Particles | 0.0281 | 0.0101 | 0.0198 | 0.0072 | 0.0093 | 0.009 | 0.042 | 0.0092 | 0.0211 | 0.015 | 0.0351 | 0.0104 | 0.0236 | 0.0135 | 0.0184 | 0.0057 | 0.0197 | 0.0082 |
Mitochondria | 0.0128 | 0.0073 | 0.0148 | 0.0058 | 0.0138 | 0.0126 | 0.0157 | 0.002 | 0.0136 | 0.0017 | 0.001 | 0.0139 | 0.0097 | 0.0016 | 0.0207 | 0.0081 | 0.0072 | 0.0065 |
None | 0.0915 | 0.08 | 0.0482 | 0.0388 | 0.0719 | 0.05 | 0.0705 | 0.0569 | 0.0394 | 0.0004 | 0.0052 | 0.0366 | 0.182 | 0.0913 | 0.0509 | 0.0349 | 0.027 | 0.0632 |
Nuclear Periphery | 0.0132 | 0.0047 | 0.0053 | 0.0138 | 0.0017 | 0.0061 | 0.008 | 0.0042 | 0.0065 | 0.0025 | 0.0111 | 0.0041 | 0.0127 | 0.0064 | 0.0037 | 0.0038 | 0.0035 | 0.0033 |
Nucleolus | 0.0021 | 0.0014 | 0.0014 | 0.0011 | 0.0015 | 0.0011 | 0.0013 | 0.0014 | 0.001 | 0.001 | 0.0024 | 0.002 | 0.0046 | 0.0019 | 0.0016 | 0.0015 | 0.0013 | 0.0012 |
Nucleus | 0.0081 | 0.0044 | 0.0031 | 0.0035 | 0.0024 | 0.0047 | 0.0047 | 0.0048 | 0.0043 | 0.0018 | 0.0176 | 0.0085 | 0.0062 | 0.0074 | 0.0031 | 0.0032 | 0.0028 | 0.0043 |
Peroxisomes | 0.004 | 0.0013 | 0.0069 | 0.0019 | 0.003 | 0.0022 | 0.0193 | 0.0007 | 0.0081 | 0.0002 | 0.0007 | 0.0034 | 0.0096 | 0.0007 | 0.003 | 0.0021 | 0.006 | 0.0011 |
Punctate Nuclear | 0.0088 | 0.001 | 0.0042 | 0.0008 | 0.0005 | 0.0013 | 0.0071 | 0.0007 | 0.0028 | 0.0001 | 0.0108 | 0.0024 | 0.0108 | 0.0008 | 0.0015 | 0.0028 | 0.0018 | 0.0004 |
Vacuole | 0.2132 | 0.3731 | 0.3075 | 0.4004 | 0.3947 | 0.4467 | 0.1761 | 0.4058 | 0.2697 | 0.5865 | 0.3093 | 0.5042 | 0.2029 | 0.4458 | 0.3436 | 0.3919 | 0.4172 | 0.4864 |
Vacuole Periphery | 0.0232 | 0.0313 | 0.0266 | 0.0446 | 0.024 | 0.0319 | 0.0101 | 0.0164 | 0.0192 | 0.0226 | 0.0173 | 0.0151 | 0.0198 | 0.0166 | 0.0284 | 0.0283 | 0.046 | 0.0317 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 223.1213 | 169.8499 | 94.8161 | 89.265 | 245.2155 | 137.6314 | 244.2104 | 161.1037 | 79.9317 | 262.2667 |
Translational Efficiency | 1.3385 | 1.3036 | 0.7201 | 0.364 | 1.2125 | 1.5549 | 0.8195 | 0.5637 | 0.8851 | 0.8752 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
265 | 1005 | 183 | 1827 | 1942 | 2048 | 1843 | 336 | 2207 | 3053 | 2026 | 2163 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 897.44 | 866.56 | 1058.55 | 918.50 | 754.15 | 811.78 | 962.49 | 946.55 | 771.36 | 829.81 | 971.17 | 922.86 |
Standard Deviation | 157.73 | 119.76 | 114.02 | 134.33 | 94.59 | 95.34 | 123.05 | 118.39 | 114.14 | 107.15 | 125.33 | 132.37 |
Intensity Change Log 2 | — | -0.050516 | 0.238202 | 0.033464 | — | 0.106237 | 0.351920 | 0.327827 | — | 0.023179 | 0.291242 | 0.175359 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.006170 | 0.009597 | 0.047699 | 0.202705* | 0.006818 | 0.006303 | 0.215072* | 0.038610 | 0.006740 | 0.007387 | 0.199954* | 0.177214* |
Bud Neck | 0.173095 | 0.100000 | 0.013433 | 0.061520 | 0.212274* | 0.121977 | 0.040112 | 0.045060 | 0.207570* | 0.114742 | 0.037703 | 0.058963 |
Bud Site | 0.196057* | 0.104882* | 0.049652 | 0.147684* | 0.082348 | 0.071778 | 0.098020 | 0.106208* | 0.096002 | 0.082676 | 0.093651 | 0.141241* |
Cell Periphery | 0.319651* | 0.010540 | 0.021755 | 0.066141 | 0.022565 | 0.007262 | 0.038004 | 0.007546 | 0.058237 | 0.008341 | 0.036536 | 0.057039 |
Cytoplasm | 0.053041 | 0.216684* | 0.198926* | 0.258592* | 0.347687* | 0.268903* | 0.260734* | 0.229356* | 0.312308* | 0.251713* | 0.255151* | 0.254050* |
Cytoplasmic Foci | 0.005128 | 0.089363 | 0.103964 | 0.026699 | 0.035340 | 0.064197 | 0.049256 | 0.113224 | 0.031712 | 0.072481 | 0.054198 | 0.040140 |
Eisosomes | 0.002727 | 0.001671 | 0.012789 | 0.033696 | 0.010776 | 0.002806 | 0.046918 | 0.004192 | 0.009809 | 0.002432 | 0.043835 | 0.029113 |
Endoplasmic Reticulum | 0.006266 | 0.005820 | 0.087795 | 0.012517 | 0.004761 | 0.003642 | 0.034029 | 0.019909 | 0.004942 | 0.004359 | 0.038886 | 0.013665 |
Endosome | 0.003919 | 0.060896 | 0.032554 | 0.011568 | 0.016035 | 0.041858 | 0.015303 | 0.048321 | 0.014580 | 0.048125 | 0.016861 | 0.017277 |
Golgi | 0.000680 | 0.008246 | 0.005241 | 0.007733 | 0.003897 | 0.007126 | 0.012682 | 0.011933 | 0.003511 | 0.007494 | 0.012009 | 0.008386 |
Lipid Particles | 0.002049 | 0.006075 | 0.031073 | 0.024179 | 0.007845 | 0.006508 | 0.040889 | 0.034042 | 0.007149 | 0.006365 | 0.040002 | 0.025711 |
Mitochondria | 0.000386 | 0.005631 | 0.000312 | 0.002890 | 0.002233 | 0.006265 | 0.001441 | 0.001243 | 0.002011 | 0.006056 | 0.001339 | 0.002634 |
Mitotic Spindle | 0.000227 | 0.003481 | 0.000463 | 0.002729 | 0.000103 | 0.001017 | 0.000627 | 0.007380 | 0.000118 | 0.001828 | 0.000613 | 0.003452 |
None | 0.000049 | 0.000630 | 0.000305 | 0.001594 | 0.000599 | 0.000468 | 0.000347 | 0.000308 | 0.000533 | 0.000521 | 0.000344 | 0.001394 |
Nuclear Periphery | 0.000039 | 0.000103 | 0.000950 | 0.000078 | 0.000099 | 0.000090 | 0.000103 | 0.000260 | 0.000092 | 0.000095 | 0.000179 | 0.000106 |
Nuclear Periphery Foci | 0.000640 | 0.000578 | 0.014570 | 0.000770 | 0.000350 | 0.000199 | 0.002045 | 0.003409 | 0.000385 | 0.000324 | 0.003177 | 0.001180 |
Nucleolus | 0.000099 | 0.000312 | 0.000236 | 0.000079 | 0.000242 | 0.000457 | 0.000104 | 0.000172 | 0.000225 | 0.000409 | 0.000116 | 0.000093 |
Nucleus | 0.004692 | 0.016340 | 0.065999 | 0.020723 | 0.033920 | 0.031468 | 0.028813 | 0.026065 | 0.030410 | 0.026488 | 0.032172 | 0.021553 |
Peroxisomes | 0.002446 | 0.013467 | 0.004020 | 0.023789 | 0.003823 | 0.010662 | 0.019305 | 0.015895 | 0.003657 | 0.011585 | 0.017924 | 0.022563 |
Vacuole | 0.221466* | 0.340889* | 0.307682* | 0.093490 | 0.207411* | 0.343790* | 0.095641 | 0.283334* | 0.209098* | 0.342835* | 0.114794 | 0.122980 |
Vacuole Periphery | 0.001174 | 0.004795 | 0.000583 | 0.000824 | 0.000875 | 0.003224 | 0.000555 | 0.003532 | 0.000911 | 0.003741 | 0.000557 | 0.001245 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.00 | -4.38 | -27.58* | -29.43* | -14.74 | 0.50 | -32.67* | -4.60 | -4.67 | 20.94* | -0.61 | -32.40* | -30.11* | -29.92* | 4.09 |
Bud Neck | 4.58 | 10.34 | 7.45 | 7.76 | -15.34 | 13.20 | 28.99* | 23.12 | 13.38 | -0.02 | 15.17 | 30.52 | 26.57 | 16.16 | -6.38 |
Bud Site | 4.80 | 7.70 | 3.05 | -4.89 | -12.25 | 2.32 | -2.70 | -1.26 | -2.39 | 0.14 | 2.91 | 0.85 | -6.21 | -10.02 | -7.45 |
Cell Periphery | 16.68 | 15.95 | 13.83 | -13.06 | -8.07 | 9.58 | -5.47 | 8.15 | 0.49 | 11.88 | 14.90 | 6.10 | 1.49 | -14.41 | -4.75 |
Cytoplasm | -16.38 | -8.73 | -23.16 | -5.20 | -4.66 | 9.65 | 10.27 | 9.29 | 3.44 | 2.98 | 8.37 | 7.09 | 7.11 | -0.85 | -0.12 |
Cytoplasmic Foci | -18.00 | -12.23 | -8.88 | 13.46 | 9.39 | -9.54 | -5.25 | -9.53 | -5.65 | -7.61 | -15.26 | -8.72 | -3.45 | 10.98 | 4.86 |
Eisosomes | 1.12 | -3.22 | -14.97 | -17.45 | -6.54 | 9.39 | -15.39 | 7.34 | -1.26 | 19.19 | 9.92 | -15.74 | -10.63 | -16.98 | 6.57 |
Endoplasmic Reticulum | 0.21 | -7.43 | -2.32 | -5.05 | 7.01 | 1.32 | -13.02 | -4.14 | -4.61 | 4.46 | 0.76 | -14.77 | -6.42 | -8.11 | 10.84 |
Endosome | -17.04 | -5.23 | -4.88 | 15.02 | 3.88 | -13.26 | 0.76 | -5.86 | -0.20 | -6.02 | -19.10 | -1.42 | -0.90 | 16.68 | 0.42 |
Golgi | -7.41 | -4.33 | -7.17 | 0.85 | -1.94 | -4.27 | -9.79 | -2.88 | -1.37 | 0.89 | -6.15 | -10.36 | -4.59 | -0.08 | 3.77 |
Lipid Particles | -3.21 | -8.43 | -10.17 | -9.41 | 2.05 | 1.81 | -14.70 | -5.35 | -5.71 | 2.11 | 1.20 | -15.87 | -10.07 | -11.02 | 6.32 |
Mitochondria | -5.39 | 0.34 | -6.08 | 2.85 | -6.19 | -5.38 | 4.48 | 2.99 | 6.32 | 0.88 | -6.73 | 4.22 | -1.22 | 5.45 | -3.35 |
Mitotic Spindle | -2.65 | -1.78 | -2.77 | 0.43 | -2.51 | -2.89 | -2.30 | -2.25 | -1.98 | -2.07 | -3.74 | -2.37 | -3.65 | -1.69 | -2.95 |
None | -1.70 | -1.96 | -2.47 | -1.31 | -2.02 | 0.87 | 1.84 | 1.79 | 0.95 | 0.24 | 0.07 | 1.55 | -1.57 | -1.56 | -1.94 |
Nuclear Periphery | -4.06 | -5.34 | -3.57 | 0.19 | 5.03 | 0.59 | -1.66 | -6.00 | -6.21 | -5.40 | -0.28 | -5.26 | -2.99 | -2.92 | 3.30 |
Nuclear Periphery Foci | 0.15 | -10.72 | -0.73 | -1.63 | 10.76 | 3.67 | -9.80 | -4.07 | -4.23 | -2.03 | 0.91 | -13.15 | -5.77 | -6.15 | 7.32 |
Nucleolus | -5.35 | -3.08 | 0.50 | 8.33 | 4.00 | -2.78 | 10.29 | 1.74 | 3.29 | -2.32 | -3.48 | 8.02 | 10.22 | 5.87 | 1.83 |
Nucleus | -3.12 | -7.31 | -5.57 | -4.49 | 5.42 | 0.94 | 1.45 | 0.75 | 0.22 | 0.08 | 1.89 | -2.29 | 1.69 | -0.15 | 3.77 |
Peroxisomes | -6.13 | -0.91 | -10.48 | -4.69 | -12.43 | -7.42 | -15.16 | -4.02 | -1.50 | 1.25 | -10.26 | -15.04 | -13.85 | -6.62 | -2.39 |
Vacuole | -5.97 | -4.35 | 5.06 | 20.27 | 11.07 | -15.61 | 15.38 | -7.15 | 1.06 | -13.72 | -17.60 | 12.56 | 6.98 | 24.71 | -5.05 |
Vacuole Periphery | -6.17 | 1.61 | 1.01 | 8.34 | -0.98 | -7.52 | 2.58 | -1.73 | 0.15 | -1.99 | -10.77 | 2.94 | -0.77 | 7.40 | -2.10 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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