Standard name
Human Ortholog
Description Mannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.06 0 0 0 0 0 0 0 0 0 0.08 0.11 0.08 0 0 0 0.05 0.07 0 0.11 0.11 0.1
Bud 0 0 0 0 0 0.05 0 0 0.05 0.06 0.07 0 0.05 0 0 0 0 0 0.05 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.07 0.06 0.08 0.06
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0.07 0 0 0 0 0 0 0 0 0
Cytoplasm 0.62 0.7 0.87 0.93 0.92 0.91 0.88 0.87 0.91 0.9 0.91 0.73 0.77 0.76 0.64 0.87 0.68 0.09 0.15 0.08 0.14 0.17 0.22
Endoplasmic Reticulum 0.09 0.17 0 0 0 0 0 0 0 0 0 0.12 0 0 0.05 0 0.06 0.09 0 0.05 0.1 0.14 0.16
Endosome 0.07 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.23 0.06 0.07 0.05 0.13 0 0.07 0 0 0 0 0 0 0 0.3 0.18 0.28 0.58 0.53 0.62 0.44 0.38 0.32
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 8 8 1 2 4 4 15 2 11 14 8 10 32 26 8 0 2 18 9 7 24 35 30
Bud 3 3 0 3 0 12 9 7 14 30 39 3 15 5 2 0 0 9 6 5 7 12 8
Bud Neck 0 1 4 2 1 0 2 0 4 3 3 0 1 0 0 0 1 13 15 12 13 24 18
Bud Site 0 2 0 0 0 0 1 1 0 2 0 0 3 0 1 0 0
Cell Periphery 1 1 1 7 2 4 14 2 9 8 10 10 18 22 6 0 0 1 2 4 5 11 12
Cytoplasm 182 87 136 199 249 224 359 184 267 418 537 87 233 234 198 157 99 32 21 15 30 55 70
Endoplasmic Reticulum 26 21 5 1 1 6 11 9 2 1 2 14 10 11 16 7 8 31 4 10 23 44 48
Endosome 21 0 0 1 3 1 3 0 1 1 2 1 15 10 9 0 2 15 0 3 3 2 3
Golgi 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 4 0 0 2 1 2
Mitochondria 3 2 0 0 0 3 6 9 10 25 25 0 1 0 6 0 3 15 6 10 11 8 10
Nucleus 3 1 1 1 0 3 3 3 2 4 6 0 1 0 12 6 9 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 0 0 1 1 0 2 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 1 0 3 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 2 0 0 0 0 0 0 0 1 0 1 1 10 12 2 0 1 0 0 0 0 0 0
SpindlePole 2 0 2 2 2 1 5 0 5 5 4 2 16 15 8 0 2 0 0 0 0 1 2
Vac/Vac Membrane 69 7 11 10 36 6 27 3 7 12 20 1 3 5 93 32 40 207 77 120 97 125 100
Unique Cell Count 294 125 156 215 272 247 410 211 294 465 590 120 304 308 307 181 145 355 147 195 223 327 314
Labelled Cell Count 321 133 161 228 298 264 456 220 334 524 661 130 360 340 361 202 167 355 147 195 223 327 314


Cytoplasm, Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 5.5 6.1 6.2 7.3 5.4 6.9 5.1 5.5 5.2 4.9 6.8 6.3 6.5 6.3 6.5 6.5 5.5 5.7 5.6
Std Deviation (1e-4) 1.2 1.3 1.4 2.2 1.8 1.3 1.7 1.1 1.6 1.5 1.1 1.6 1.7 2.1 1.3 1.6 1.6 1.3 1.7 1.7
Intensity Change (Log2) 0.03 0.26 -0.18 0.17 -0.25 -0.14 -0.22 -0.33 0.15 0.06 0.08 0.04 0.1 0.09 -0.15 -0.1 -0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 3.9 3.4 0 0 0
Bud 0 0 0 0 0 0 3.3 0 0 2.8 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 2.7 3.0 0 0 0
Cytoplasm 1.7 1.4 1.1 0.1 0 1.2 0.9 1.4 -3.1 -2.7 -2.8 -5.1 -0.1 -4.0
Endoplasmic Reticulum 0 0 0 0 0.5 0 0 0 2.8 0 0.2 1.0 0.3 1.0
Endosome 0 0 0 0 0 0 0 0 0 2.8 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 3.0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 2.1 1.9 0 0 0
Vacuole -1.0 2.0 -2.2 -0.2 -2.8 -2.4 -2.6 -2.0 -2.5 0 -3.0 5.7 2.9 4.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.6323 0.369 0.2292 -0.0188 -0.2333 0.1224 1.9222 2.1634 2.4998 2.4874 1.8249 2.2861 2.2226 3.4744 3.2069 3.1371 2.991 3.017
Actin 0.0138 0.0015 0.0083 0.0013 0.0005 0.004 0.0302 0.0038 0.0143 0 0.0027 0.002 0.0162 0.0006 0.0171 0 0.0211 0.0004
Bud 0.0013 0.0015 0.002 0.0009 0.0008 0.0006 0.0011 0.0021 0.0046 0.0003 0.0003 0.0007 0.0014 0.0014 0.0008 0.0005 0.0019 0.0025
Bud Neck 0.002 0.0023 0.0029 0.0022 0.0015 0.0066 0.0041 0.0075 0.004 0.0017 0.0031 0.0145 0.0016 0.0022 0.0068 0.0011 0.0018 0.0072
Bud Periphery 0.0019 0.0028 0.0055 0.0017 0.0008 0.0016 0.0025 0.0034 0.0061 0.0007 0.0004 0.002 0.0044 0.0031 0.0019 0.0007 0.0077 0.0043
Bud Site 0.0054 0.0054 0.003 0.0012 0.0065 0.0012 0.0097 0.02 0.0231 0.0014 0.0017 0.0021 0.004 0.0114 0.0048 0.0012 0.0029 0.0021
Cell Periphery 0.0025 0.0027 0.0017 0.0021 0.001 0.003 0.0055 0.0054 0.0026 0.0036 0.0009 0.0053 0.0026 0.004 0.003 0.0007 0.0017 0.0033
Cytoplasm 0.1183 0.1361 0.0723 0.0431 0.0467 0.0826 0.1886 0.1903 0.1465 0.0551 0.0679 0.1471 0.1179 0.1652 0.115 0.0641 0.027 0.1221
Cytoplasmic Foci 0.0838 0.054 0.0639 0.0704 0.0478 0.0418 0.0905 0.0445 0.0697 0.0125 0.0502 0.0391 0.075 0.0329 0.054 0.0547 0.0271 0.0334
Eisosomes 0.0007 0.0001 0.0002 0 0.0001 0.0001 0.0005 0.0001 0.0001 0 0.0001 0 0.0004 0 0.0001 0 0.0002 0
Endoplasmic Reticulum 0.0167 0.0108 0.0164 0.0068 0.0032 0.0091 0.029 0.0172 0.0154 0.006 0.009 0.0138 0.0146 0.0201 0.01 0.0039 0.0067 0.0095
Endosome 0.3297 0.2619 0.3727 0.3363 0.3452 0.2731 0.2562 0.1893 0.3057 0.2769 0.4395 0.1622 0.2669 0.1668 0.2997 0.3804 0.3571 0.2022
Golgi 0.0186 0.0063 0.0134 0.0163 0.0231 0.0107 0.0272 0.0146 0.0224 0.01 0.0138 0.0105 0.0133 0.0063 0.0117 0.0105 0.0122 0.0068
Lipid Particles 0.0281 0.0101 0.0198 0.0072 0.0093 0.009 0.042 0.0092 0.0211 0.015 0.0351 0.0104 0.0236 0.0135 0.0184 0.0057 0.0197 0.0082
Mitochondria 0.0128 0.0073 0.0148 0.0058 0.0138 0.0126 0.0157 0.002 0.0136 0.0017 0.001 0.0139 0.0097 0.0016 0.0207 0.0081 0.0072 0.0065
None 0.0915 0.08 0.0482 0.0388 0.0719 0.05 0.0705 0.0569 0.0394 0.0004 0.0052 0.0366 0.182 0.0913 0.0509 0.0349 0.027 0.0632
Nuclear Periphery 0.0132 0.0047 0.0053 0.0138 0.0017 0.0061 0.008 0.0042 0.0065 0.0025 0.0111 0.0041 0.0127 0.0064 0.0037 0.0038 0.0035 0.0033
Nucleolus 0.0021 0.0014 0.0014 0.0011 0.0015 0.0011 0.0013 0.0014 0.001 0.001 0.0024 0.002 0.0046 0.0019 0.0016 0.0015 0.0013 0.0012
Nucleus 0.0081 0.0044 0.0031 0.0035 0.0024 0.0047 0.0047 0.0048 0.0043 0.0018 0.0176 0.0085 0.0062 0.0074 0.0031 0.0032 0.0028 0.0043
Peroxisomes 0.004 0.0013 0.0069 0.0019 0.003 0.0022 0.0193 0.0007 0.0081 0.0002 0.0007 0.0034 0.0096 0.0007 0.003 0.0021 0.006 0.0011
Punctate Nuclear 0.0088 0.001 0.0042 0.0008 0.0005 0.0013 0.0071 0.0007 0.0028 0.0001 0.0108 0.0024 0.0108 0.0008 0.0015 0.0028 0.0018 0.0004
Vacuole 0.2132 0.3731 0.3075 0.4004 0.3947 0.4467 0.1761 0.4058 0.2697 0.5865 0.3093 0.5042 0.2029 0.4458 0.3436 0.3919 0.4172 0.4864
Vacuole Periphery 0.0232 0.0313 0.0266 0.0446 0.024 0.0319 0.0101 0.0164 0.0192 0.0226 0.0173 0.0151 0.0198 0.0166 0.0284 0.0283 0.046 0.0317

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 223.1213 169.8499 94.8161 89.265 245.2155 137.6314 244.2104 161.1037 79.9317 262.2667
Translational Efficiency 1.3385 1.3036 0.7201 0.364 1.2125 1.5549 0.8195 0.5637 0.8851 0.8752

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
265 1005 183 1827 1942 2048 1843 336 2207 3053 2026 2163

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 897.44 866.56 1058.55 918.50 754.15 811.78 962.49 946.55 771.36 829.81 971.17 922.86
Standard Deviation 157.73 119.76 114.02 134.33 94.59 95.34 123.05 118.39 114.14 107.15 125.33 132.37
Intensity Change Log 2 -0.050516 0.238202 0.033464 0.106237 0.351920 0.327827 0.023179 0.291242 0.175359

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.006170 0.009597 0.047699 0.202705* 0.006818 0.006303 0.215072* 0.038610 0.006740 0.007387 0.199954* 0.177214*
Bud Neck 0.173095 0.100000 0.013433 0.061520 0.212274* 0.121977 0.040112 0.045060 0.207570* 0.114742 0.037703 0.058963
Bud Site 0.196057* 0.104882* 0.049652 0.147684* 0.082348 0.071778 0.098020 0.106208* 0.096002 0.082676 0.093651 0.141241*
Cell Periphery 0.319651* 0.010540 0.021755 0.066141 0.022565 0.007262 0.038004 0.007546 0.058237 0.008341 0.036536 0.057039
Cytoplasm 0.053041 0.216684* 0.198926* 0.258592* 0.347687* 0.268903* 0.260734* 0.229356* 0.312308* 0.251713* 0.255151* 0.254050*
Cytoplasmic Foci 0.005128 0.089363 0.103964 0.026699 0.035340 0.064197 0.049256 0.113224 0.031712 0.072481 0.054198 0.040140
Eisosomes 0.002727 0.001671 0.012789 0.033696 0.010776 0.002806 0.046918 0.004192 0.009809 0.002432 0.043835 0.029113
Endoplasmic Reticulum 0.006266 0.005820 0.087795 0.012517 0.004761 0.003642 0.034029 0.019909 0.004942 0.004359 0.038886 0.013665
Endosome 0.003919 0.060896 0.032554 0.011568 0.016035 0.041858 0.015303 0.048321 0.014580 0.048125 0.016861 0.017277
Golgi 0.000680 0.008246 0.005241 0.007733 0.003897 0.007126 0.012682 0.011933 0.003511 0.007494 0.012009 0.008386
Lipid Particles 0.002049 0.006075 0.031073 0.024179 0.007845 0.006508 0.040889 0.034042 0.007149 0.006365 0.040002 0.025711
Mitochondria 0.000386 0.005631 0.000312 0.002890 0.002233 0.006265 0.001441 0.001243 0.002011 0.006056 0.001339 0.002634
Mitotic Spindle 0.000227 0.003481 0.000463 0.002729 0.000103 0.001017 0.000627 0.007380 0.000118 0.001828 0.000613 0.003452
None 0.000049 0.000630 0.000305 0.001594 0.000599 0.000468 0.000347 0.000308 0.000533 0.000521 0.000344 0.001394
Nuclear Periphery 0.000039 0.000103 0.000950 0.000078 0.000099 0.000090 0.000103 0.000260 0.000092 0.000095 0.000179 0.000106
Nuclear Periphery Foci 0.000640 0.000578 0.014570 0.000770 0.000350 0.000199 0.002045 0.003409 0.000385 0.000324 0.003177 0.001180
Nucleolus 0.000099 0.000312 0.000236 0.000079 0.000242 0.000457 0.000104 0.000172 0.000225 0.000409 0.000116 0.000093
Nucleus 0.004692 0.016340 0.065999 0.020723 0.033920 0.031468 0.028813 0.026065 0.030410 0.026488 0.032172 0.021553
Peroxisomes 0.002446 0.013467 0.004020 0.023789 0.003823 0.010662 0.019305 0.015895 0.003657 0.011585 0.017924 0.022563
Vacuole 0.221466* 0.340889* 0.307682* 0.093490 0.207411* 0.343790* 0.095641 0.283334* 0.209098* 0.342835* 0.114794 0.122980
Vacuole Periphery 0.001174 0.004795 0.000583 0.000824 0.000875 0.003224 0.000555 0.003532 0.000911 0.003741 0.000557 0.001245

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.00 -4.38 -27.58* -29.43* -14.74 0.50 -32.67* -4.60 -4.67 20.94* -0.61 -32.40* -30.11* -29.92* 4.09
Bud Neck 4.58 10.34 7.45 7.76 -15.34 13.20 28.99* 23.12 13.38 -0.02 15.17 30.52 26.57 16.16 -6.38
Bud Site 4.80 7.70 3.05 -4.89 -12.25 2.32 -2.70 -1.26 -2.39 0.14 2.91 0.85 -6.21 -10.02 -7.45
Cell Periphery 16.68 15.95 13.83 -13.06 -8.07 9.58 -5.47 8.15 0.49 11.88 14.90 6.10 1.49 -14.41 -4.75
Cytoplasm -16.38 -8.73 -23.16 -5.20 -4.66 9.65 10.27 9.29 3.44 2.98 8.37 7.09 7.11 -0.85 -0.12
Cytoplasmic Foci -18.00 -12.23 -8.88 13.46 9.39 -9.54 -5.25 -9.53 -5.65 -7.61 -15.26 -8.72 -3.45 10.98 4.86
Eisosomes 1.12 -3.22 -14.97 -17.45 -6.54 9.39 -15.39 7.34 -1.26 19.19 9.92 -15.74 -10.63 -16.98 6.57
Endoplasmic Reticulum 0.21 -7.43 -2.32 -5.05 7.01 1.32 -13.02 -4.14 -4.61 4.46 0.76 -14.77 -6.42 -8.11 10.84
Endosome -17.04 -5.23 -4.88 15.02 3.88 -13.26 0.76 -5.86 -0.20 -6.02 -19.10 -1.42 -0.90 16.68 0.42
Golgi -7.41 -4.33 -7.17 0.85 -1.94 -4.27 -9.79 -2.88 -1.37 0.89 -6.15 -10.36 -4.59 -0.08 3.77
Lipid Particles -3.21 -8.43 -10.17 -9.41 2.05 1.81 -14.70 -5.35 -5.71 2.11 1.20 -15.87 -10.07 -11.02 6.32
Mitochondria -5.39 0.34 -6.08 2.85 -6.19 -5.38 4.48 2.99 6.32 0.88 -6.73 4.22 -1.22 5.45 -3.35
Mitotic Spindle -2.65 -1.78 -2.77 0.43 -2.51 -2.89 -2.30 -2.25 -1.98 -2.07 -3.74 -2.37 -3.65 -1.69 -2.95
None -1.70 -1.96 -2.47 -1.31 -2.02 0.87 1.84 1.79 0.95 0.24 0.07 1.55 -1.57 -1.56 -1.94
Nuclear Periphery -4.06 -5.34 -3.57 0.19 5.03 0.59 -1.66 -6.00 -6.21 -5.40 -0.28 -5.26 -2.99 -2.92 3.30
Nuclear Periphery Foci 0.15 -10.72 -0.73 -1.63 10.76 3.67 -9.80 -4.07 -4.23 -2.03 0.91 -13.15 -5.77 -6.15 7.32
Nucleolus -5.35 -3.08 0.50 8.33 4.00 -2.78 10.29 1.74 3.29 -2.32 -3.48 8.02 10.22 5.87 1.83
Nucleus -3.12 -7.31 -5.57 -4.49 5.42 0.94 1.45 0.75 0.22 0.08 1.89 -2.29 1.69 -0.15 3.77
Peroxisomes -6.13 -0.91 -10.48 -4.69 -12.43 -7.42 -15.16 -4.02 -1.50 1.25 -10.26 -15.04 -13.85 -6.62 -2.39
Vacuole -5.97 -4.35 5.06 20.27 11.07 -15.61 15.38 -7.15 1.06 -13.72 -17.60 12.56 6.98 24.71 -5.05
Vacuole Periphery -6.17 1.61 1.01 8.34 -0.98 -7.52 2.58 -1.73 0.15 -1.99 -10.77 2.94 -0.77 7.40 -2.10