Standard name
Description Mannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.06 0 0 0 0 0 0 0 0 0 0.08 0.11 0.08 0 0 0 0.05 0.07 0 0.11 0.11 0.10
Bud 0 0 0 0 0 0.05 0 0 0.05 0.06 0.07 0 0.05 0 0 0 0 0 0.05 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.07 0.06 0.08 0.06
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0.07 0 0 0 0 0 0 0 0 0
Cytoplasm 0.62 0.70 0.87 0.93 0.92 0.91 0.88 0.87 0.91 0.90 0.91 0.73 0.77 0.76 0.64 0.87 0.68 0.09 0.15 0.08 0.14 0.17 0.22
Endoplasmic Reticulum 0.09 0.17 0 0 0 0 0 0 0 0 0 0.12 0 0 0.05 0 0.06 0.09 0 0.05 0.10 0.14 0.16
Endosome 0.07 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.23 0.06 0.07 0.05 0.13 0 0.07 0 0 0 0 0 0 0 0.30 0.18 0.28 0.58 0.53 0.62 0.44 0.38 0.32
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 8 8 1 2 4 4 15 2 11 14 8 10 32 26 8 0 2 18 9 7 24 35 30
Bud 3 3 0 3 0 12 9 7 14 30 39 3 15 5 2 0 0 9 6 5 7 12 8
Bud Neck 0 1 4 2 1 0 2 0 4 3 3 0 1 0 0 0 1 13 15 12 13 24 18
Bud Site 0 2 0 0 0 0 1 1 0 2 0 0 3 0 1 0 0
Cell Periphery 1 1 1 7 2 4 14 2 9 8 10 10 18 22 6 0 0 1 2 4 5 11 12
Cytoplasm 182 87 136 199 249 224 359 184 267 418 537 87 233 234 198 157 99 32 21 15 30 55 70
Endoplasmic Reticulum 26 21 5 1 1 6 11 9 2 1 2 14 10 11 16 7 8 31 4 10 23 44 48
Endosome 21 0 0 1 3 1 3 0 1 1 2 1 15 10 9 0 2 15 0 3 3 2 3
Golgi 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 4 0 0 2 1 2
Mitochondria 3 2 0 0 0 3 6 9 10 25 25 0 1 0 6 0 3 15 6 10 11 8 10
Nucleus 3 1 1 1 0 3 3 3 2 4 6 0 1 0 12 6 9 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 0 0 1 1 0 2 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 1 0 3 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 2 0 0 0 0 0 0 0 1 0 1 1 10 12 2 0 1 0 0 0 0 0 0
SpindlePole 2 0 2 2 2 1 5 0 5 5 4 2 16 15 8 0 2 0 0 0 0 1 2
Vac/Vac Membrane 69 7 11 10 36 6 27 3 7 12 20 1 3 5 93 32 40 207 77 120 97 125 100
Unique Cell Count 294 125 156 215 272 247 410 211 294 465 590 120 304 308 307 181 145 355 147 195 223 327 314
Labelled Cell Count 321 133 161 228 298 264 456 220 334 524 661 130 360 340 361 202 167 355 147 195 223 327 314

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Cytoplasm, Vacuole

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 5.5 6.1 6.2 7.3 5.4 6.9 5.1 5.5 5.2 4.9 6.8 6.3 6.5 6.3 6.5 6.5 5.5 5.7 5.6
Std Deviation (1e-4) 1.2 1.3 1.4 2.2 1.8 1.3 1.7 1.1 1.6 1.5 1.1 1.6 1.7 2.1 1.3 1.6 1.6 1.3 1.7 1.7
Intensity Change (Log2) 0.0 0.3 -0.2 0.2 -0.2 -0.1 -0.2 -0.3 0.1 0.1 0.1 0.0 0.1 0.1 -0.2 -0.1 -0.1

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 3.9 3.4 0 0 0
Bud 0 0 0 0 0 0 3.3 0 0 2.8 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 2.7 3.0 0 0 0
Cytoplasm -3.1 -2.0 -2.2 -1.4 -1.5 -3.8 -4.8 0 1.1 -2.0 -2.3 -1.6 1.9 0.1
Endoplasmic Reticulum 0 0 0 0 0.5 0 0 0 2.8 0 0.2 1.0 0.3 1.0
Endosome 0 0 0 0 0 0 0 0 0 2.8 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 3.0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 2.1 1.9 0 0 0
Vacuole -1.4 1.5 -2.5 -2.6 -3.1 -2.9 -3.1 0 -2.5 0 -3.3 5.3 2.9 4.7