ORF
Description Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.09 0 0 0 0 0 0 0 0 0
Bud 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.07 0.05 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0 0 0 0
Cytoplasm 0.38 0.54 0.50 0.69 0.66 0.80 0.75 0.83 0.82 0.85 0.87 0.72 0.71 0.80 0.25 0.36 0.30 0.06 0.06 0.12 0.07 0.09 0.09
Endoplasmic Reticulum 0.09 0 0.05 0 0.05 0.05 0 0 0 0 0 0.14 0.19 0 0 0.06 0 0 0 0 0 0.07 0.07
Endosome 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.15 0.09 0 0.05 0.05 0.12 0.09 0.14 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.09 0.05 0.10 0 0 0 0 0 0 0 0 0 0 0 0.17 0.20 0.17 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.50 0.48 0.15 0.22 0.32 0.16 0.18 0.13 0.11 0.09 0.08 0.14 0 0 0.63 0.47 0.53 0.80 0.81 0.76 0.71 0.69 0.70
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 0 0 0 1 7 5 2 3 0 2 2 36 49 0 3 1 3 1 0 1 7 8
Bud 0 1 1 4 4 5 21 17 19 10 22 2 12 19 0 0 0 17 5 2 4 16 15
Bud Neck 0 0 0 0 1 0 1 0 1 0 1 0 0 2 0 0 0 2 1 0 1 4 9
Bud Site 0 0 0 0 0 0 0 1 0 3 2 0 3 0 0 0 1
Cell Periphery 0 0 0 0 3 2 1 3 3 1 3 2 26 30 0 0 0 0 0 0 0 1 2
Cytoplasm 104 117 10 161 279 298 639 427 511 368 450 135 324 411 21 38 21 19 13 8 4 34 37
Endoplasmic Reticulum 24 4 1 4 19 20 18 2 6 3 0 26 89 23 3 6 3 5 1 0 1 27 28
Endosome 4 11 0 4 5 0 1 1 1 0 0 3 0 4 2 1 3 9 10 2 3 2 6
Golgi 2 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 2 4 1 0 0 2 2
Mitochondria 10 4 3 21 15 18 46 62 58 59 65 2 3 1 1 2 3 3 3 0 0 13 9
Nucleus 26 10 2 10 11 3 17 3 11 5 5 0 0 0 14 21 12 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 1 1 5 5 6 6 6 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 1 0 0 0 0 1 3 1 4 3 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 0 0 1 1 0 0 0 5 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 6 4 3 3 3 1 5 21 1 0 1 1 0 0 0 2 2
Vac/Vac Membrane 139 103 3 52 133 58 153 69 70 38 40 26 14 8 52 49 37 265 189 53 50 252 292
Unique Cell Count 276 215 20 234 421 371 847 514 624 433 520 187 458 516 83 105 70 333 233 70 70 366 419
Labelled Cell Count 314 251 20 256 473 413 914 599 694 501 602 199 513 573 95 121 84 333 233 70 70 366 419

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Vacuole

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 6.5 4.6 4.5 5.7 4.9 5.0 4.4 4.4 4.5 4.0 6.5 6.3 5.8 9.3 8.7 9.3 5.9 6.8 6.5
Std Deviation (1e-4) 1.3 2.2 0.8 1.3 1.4 1.3 1.5 1.6 1.3 7.1 1.1 1.7 1.3 1.4 2.6 2.2 2.6 1.2 1.8 1.6
Intensity Change (Log2) -0.06 0.3 0.09 0.1 -0.08 -0.07 -0.03 -0.22 0.48 0.45 0.33 1.0 0.9 1.0 0.35 0.55 0.48

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.7 1.5 3.2 2.6 3.8 3.6 4.1 4.5 2.1 2.0 3.2 -2.2 -1.2 -1.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.6 0 0 0 0 0 0 0 0 0 3.8 2.6 3.0