ORF | |
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Human Ortholog | |
Description | Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 5 | 0 | 0 | 0 | 1 | 7 | – | 5 | 2 | 3 | 0 | 2 | 2 | 36 | 49 | 0 | 3 | 1 | 3 | 1 | 0 | 1 | 7 | 8 |
Bud | 0 | 1 | 1 | 4 | 4 | 5 | – | 21 | 17 | 19 | 10 | 22 | 2 | 12 | 19 | 0 | 0 | 0 | 17 | 5 | 2 | 4 | 16 | 15 |
Bud Neck | 0 | 0 | 0 | 0 | 1 | 0 | – | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 2 | 1 | 0 | 1 | 4 | 9 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 3 | 2 | 0 | 3 | 0 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 3 | 2 | – | 1 | 3 | 3 | 1 | 3 | 2 | 26 | 30 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 |
Cytoplasm | 104 | 117 | 10 | 161 | 279 | 298 | – | 639 | 427 | 511 | 368 | 450 | 135 | 324 | 411 | 21 | 38 | 21 | 19 | 13 | 8 | 4 | 34 | 37 |
Endoplasmic Reticulum | 24 | 4 | 1 | 4 | 19 | 20 | – | 18 | 2 | 6 | 3 | 0 | 26 | 89 | 23 | 3 | 6 | 3 | 5 | 1 | 0 | 1 | 27 | 28 |
Endosome | 4 | 11 | 0 | 4 | 5 | 0 | – | 1 | 1 | 1 | 0 | 0 | 3 | 0 | 4 | 2 | 1 | 3 | 9 | 10 | 2 | 3 | 2 | 6 |
Golgi | 2 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 2 | 4 | 1 | 0 | 0 | 2 | 2 |
Mitochondria | 10 | 4 | 3 | 21 | 15 | 18 | – | 46 | 62 | 58 | 59 | 65 | 2 | 3 | 1 | 1 | 2 | 3 | 3 | 3 | 0 | 0 | 13 | 9 |
Nucleus | 26 | 10 | 2 | 10 | 11 | 3 | – | 17 | 3 | 11 | 5 | 5 | 0 | 0 | 0 | 14 | 21 | 12 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 1 | 1 | – | 5 | 5 | 6 | 6 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 1 | 0 | 0 | 0 | 0 | – | 1 | 3 | 1 | 4 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 1 | – | 6 | 4 | 3 | 3 | 3 | 1 | 5 | 21 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 2 | 2 |
Vac/Vac Membrane | 139 | 103 | 3 | 52 | 133 | 58 | – | 153 | 69 | 70 | 38 | 40 | 26 | 14 | 8 | 52 | 49 | 37 | 265 | 189 | 53 | 50 | 252 | 292 |
Unique Cell Count | 276 | 215 | 20 | 234 | 421 | 371 | 847 | 514 | 624 | 433 | 520 | 187 | 458 | 516 | 83 | 105 | 70 | 333 | 233 | 70 | 70 | 366 | 419 | |
Labelled Cell Count | 314 | 251 | 20 | 256 | 473 | 413 | 914 | 599 | 694 | 501 | 602 | 199 | 513 | 573 | 95 | 121 | 84 | 333 | 233 | 70 | 70 | 366 | 419 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.9 | 6.5 | 4.6 | 4.5 | 5.7 | 4.9 | – | 5.0 | 4.4 | 4.4 | 4.5 | 4.0 | 6.5 | 6.3 | 5.8 | 9.3 | 8.7 | 9.3 | 5.9 | 6.8 | 6.5 |
Std Deviation (1e-4) | 1.3 | 2.2 | 0.8 | 1.3 | 1.4 | 1.3 | – | 1.5 | 1.6 | 1.3 | 7.1 | 1.1 | 1.7 | 1.3 | 1.4 | 2.6 | 2.2 | 2.6 | 1.2 | 1.8 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.06 | 0.3 | 0.09 | – | 0.1 | -0.08 | -0.07 | -0.03 | -0.22 | 0.48 | 0.45 | 0.33 | 1.0 | 0.9 | 1.0 | 0.35 | 0.55 | 0.48 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.7 | 1.5 | 3.2 | – | 2.6 | 3.8 | 3.6 | 4.1 | 4.5 | 2.1 | 2.0 | 3.2 | -2.2 | -1.2 | -1.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 1.6 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.8 | 2.6 | 3.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.85 | 1.6601 | 1.7233 | 1.5794 | 1.6513 | 1.6833 | 0.5504 | 2.2807 | 2.2141 | 1.4615 | 1.6369 | 1.9725 | 1.6294 | 3.589 | 3.748 | 3.0298 | 2.5923 | 3.1013 |
Actin | 0.072 | 0 | 0.0185 | 0.0175 | 0.0354 | 0.002 | 0.0323 | 0.0008 | 0.0094 | 0.0016 | 0.0001 | 0.0078 | 0.0504 | 0.0001 | 0.002 | 0.0051 | 0.04 | 0.0116 |
Bud | 0.0007 | 0.0003 | 0.0008 | 0.0004 | 0.0006 | 0.0008 | 0.0012 | 0.0008 | 0.0012 | 0.0005 | 0.0011 | 0.0005 | 0.0009 | 0.0008 | 0.0005 | 0.0006 | 0.0003 | 0.0004 |
Bud Neck | 0.0025 | 0.0014 | 0.0065 | 0.0011 | 0.0031 | 0.0053 | 0.0013 | 0.003 | 0.0027 | 0.0011 | 0.0019 | 0.0031 | 0.0038 | 0.0028 | 0.0018 | 0.0014 | 0.0008 | 0.0017 |
Bud Periphery | 0.0016 | 0.0008 | 0.0018 | 0.0008 | 0.0013 | 0.0029 | 0.0026 | 0.0019 | 0.002 | 0.0009 | 0.0025 | 0.0017 | 0.0016 | 0.0014 | 0.001 | 0.0008 | 0.0005 | 0.0012 |
Bud Site | 0.0025 | 0.0019 | 0.0065 | 0.0024 | 0.0016 | 0.0012 | 0.0056 | 0.0052 | 0.0052 | 0.0008 | 0.001 | 0.0011 | 0.0031 | 0.0027 | 0.0068 | 0.0041 | 0.0008 | 0.0008 |
Cell Periphery | 0.0028 | 0.003 | 0.0021 | 0.0027 | 0.0021 | 0.0033 | 0.0048 | 0.0053 | 0.0036 | 0.0016 | 0.0043 | 0.0081 | 0.001 | 0.0023 | 0.0011 | 0.0009 | 0.0004 | 0.0021 |
Cytoplasm | 0.0337 | 0.0226 | 0.0236 | 0.0041 | 0.0055 | 0.0289 | 0.0454 | 0.0434 | 0.023 | 0.0608 | 0.0258 | 0.0242 | 0.0765 | 0.0797 | 0.0226 | 0.0041 | 0.0067 | 0.0155 |
Cytoplasmic Foci | 0.0335 | 0.0168 | 0.0226 | 0.0129 | 0.0086 | 0.0144 | 0.0496 | 0.0265 | 0.0403 | 0.0266 | 0.0782 | 0.0212 | 0.038 | 0.015 | 0.0234 | 0.0299 | 0.0136 | 0.0083 |
Eisosomes | 0.0005 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0123 | 0.01 | 0.0057 | 0.0015 | 0.0054 | 0.0054 | 0.0135 | 0.0215 | 0.0141 | 0.0042 | 0.0072 | 0.0071 | 0.0202 | 0.0167 | 0.0083 | 0.004 | 0.002 | 0.009 |
Endosome | 0.2542 | 0.161 | 0.2608 | 0.1417 | 0.2064 | 0.1914 | 0.3591 | 0.2236 | 0.3805 | 0.3306 | 0.3136 | 0.1916 | 0.3309 | 0.1779 | 0.3128 | 0.3943 | 0.2577 | 0.1416 |
Golgi | 0.0113 | 0.0038 | 0.0101 | 0.0053 | 0.0405 | 0.0052 | 0.0248 | 0.0082 | 0.0329 | 0.0303 | 0.0223 | 0.0104 | 0.0152 | 0.0053 | 0.012 | 0.0285 | 0.0129 | 0.0168 |
Lipid Particles | 0.0234 | 0.0056 | 0.0119 | 0.0174 | 0.0204 | 0.0078 | 0.0344 | 0.013 | 0.0252 | 0.0041 | 0.0077 | 0.0093 | 0.0375 | 0.0073 | 0.0183 | 0.0103 | 0.0081 | 0.0068 |
Mitochondria | 0.007 | 0.0017 | 0.0214 | 0.0056 | 0.011 | 0.0156 | 0.014 | 0.0049 | 0.0181 | 0.0048 | 0.0071 | 0.0106 | 0.0152 | 0.002 | 0.03 | 0.01 | 0.0014 | 0.003 |
None | 0.0966 | 0.016 | 0.0024 | 0.0041 | 0.001 | 0.0102 | 0.0205 | 0.0237 | 0.0185 | 0.0018 | 0.0017 | 0.0007 | 0.0518 | 0.019 | 0.0023 | 0.0005 | 0.0006 | 0.0002 |
Nuclear Periphery | 0.016 | 0.0081 | 0.0051 | 0.0031 | 0.0341 | 0.0139 | 0.009 | 0.0095 | 0.0049 | 0.0024 | 0.0022 | 0.0032 | 0.0158 | 0.0116 | 0.007 | 0.0035 | 0.0038 | 0.0066 |
Nucleolus | 0.0017 | 0.0022 | 0.0032 | 0.0025 | 0.002 | 0.0031 | 0.0017 | 0.0016 | 0.0012 | 0.0005 | 0.0005 | 0.0095 | 0.0016 | 0.0016 | 0.0018 | 0.0009 | 0.0006 | 0.001 |
Nucleus | 0.0053 | 0.0041 | 0.0054 | 0.0023 | 0.0082 | 0.0159 | 0.0028 | 0.0035 | 0.009 | 0.0019 | 0.0012 | 0.0063 | 0.0041 | 0.0043 | 0.0054 | 0.0014 | 0.0013 | 0.0128 |
Peroxisomes | 0.0194 | 0.0005 | 0.0028 | 0.0561 | 0.0028 | 0.0012 | 0.0079 | 0.0006 | 0.0024 | 0.0008 | 0.0022 | 0.0022 | 0.0041 | 0.0004 | 0.0045 | 0.0061 | 0.0034 | 0.006 |
Punctate Nuclear | 0.006 | 0.0004 | 0.0026 | 0.001 | 0.0027 | 0.0055 | 0.0066 | 0.0003 | 0.0009 | 0.0002 | 0.0006 | 0.0005 | 0.0036 | 0.0003 | 0.0081 | 0.0005 | 0.0027 | 0.0004 |
Vacuole | 0.3318 | 0.6528 | 0.4876 | 0.6537 | 0.5567 | 0.5931 | 0.3376 | 0.5669 | 0.3576 | 0.4689 | 0.4674 | 0.6285 | 0.2736 | 0.5887 | 0.4302 | 0.3885 | 0.555 | 0.6568 |
Vacuole Periphery | 0.0652 | 0.0867 | 0.0984 | 0.0637 | 0.0503 | 0.0729 | 0.0251 | 0.0359 | 0.0472 | 0.0555 | 0.0511 | 0.0523 | 0.0507 | 0.0602 | 0.1002 | 0.1043 | 0.0876 | 0.0973 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 234.8831 | 143.2361 | 79.7155 | 98.4768 | 247.8783 | 134.6646 | 245.2166 | 167.7262 | 78.2747 | 294.6677 |
Translational Efficiency | 1.307 | 1.5221 | 0.9427 | 0.2972 | 1.1465 | 1.3534 | 0.761 | 0.5631 | 0.7504 | 0.6164 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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