Standard name | |
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Description | Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud | 0 | 1 | 0 | 1 | 1 | 0 | 1 | – | 4 | 5 | 8 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 7 | 3 | 1 | 3 | 4 |
Bud Neck | 0 | 0 | 0 | 2 | 0 | 1 | 2 | – | 1 | 4 | 2 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 1 | 0 | 5 | 4 | – | 5 | 6 | 13 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 7 | 20 | 22 | 20 | 14 | 23 | 38 | – | 34 | 26 | 35 | 46 | 29 | 42 | 63 | 15 | 31 | 18 | 1 | 7 | 6 | 7 | 7 | 11 |
Endoplasmic Reticulum | 1 | 1 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 13 | 3 | 0 | 0 | 1 | 0 | 0 | 0 |
Endosome | 14 | 28 | 15 | 6 | 6 | 15 | 7 | – | 4 | 5 | 4 | 12 | 13 | 26 | 42 | 17 | 49 | 25 | 14 | 23 | 6 | 0 | 3 | 8 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 4 | 2 | 0 | 1 | 2 | 0 | 1 | 0 |
Mitochondria | 24 | 7 | 0 | 4 | 5 | 38 | 42 | – | 105 | 101 | 177 | 102 | 2 | 2 | 0 | 9 | 10 | 8 | 7 | 4 | 3 | 2 | 8 | 9 |
Nucleus | 225 | 219 | 103 | 49 | 43 | 75 | 75 | – | 117 | 83 | 98 | 80 | 103 | 129 | 98 | 83 | 105 | 84 | 194 | 206 | 98 | 109 | 145 | 180 |
Nuclear Periphery | 3 | 2 | 0 | 0 | 0 | 0 | 1 | – | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 2 | 0 | 0 | 0 |
Nucleolus | 31 | 50 | 12 | 16 | 18 | 31 | 21 | – | 17 | 14 | 11 | 10 | 18 | 36 | 43 | 25 | 27 | 21 | 15 | 19 | 16 | 1 | 6 | 13 |
Peroxisomes | 0 | 0 | 1 | 0 | 0 | 3 | 0 | – | 0 | 0 | 0 | 0 | 2 | 0 | 3 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 11 | 37 | 14 | 31 | 38 | 49 | 34 | – | 23 | 16 | 14 | 25 | 21 | 50 | 53 | 31 | 34 | 35 | 31 | 65 | 26 | 2 | 5 | 14 |
Vac/Vac Membrane | 16 | 43 | 9 | 5 | 11 | 11 | 10 | – | 13 | 20 | 22 | 25 | 7 | 9 | 11 | 19 | 24 | 27 | 23 | 29 | 24 | 17 | 33 | 58 |
Unique Cell Count | 278 | 334 | 159 | 110 | 112 | 195 | 188 | 250 | 217 | 269 | 240 | 167 | 242 | 250 | 169 | 226 | 173 | 300 | 376 | 205 | 147 | 222 | 310 | |
Labelled Cell Count | 332 | 408 | 177 | 136 | 136 | 251 | 235 | 323 | 281 | 385 | 315 | 195 | 298 | 314 | 206 | 300 | 226 | 300 | 376 | 205 | 147 | 222 | 310 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 4.9 | 6.0 | 6.8 | 6.6 | 6.2 | 5.3 | 5.8 | – | 4.4 | 4.6 | 4.1 | 4.7 | 6.2 | 6.2 | 6.1 | 6.9 | 7.2 | 6.6 | 5.4 | 6.1 | 6.1 |
Std Deviation (1e-4) | 0.8 | 0.9 | 2.1 | 2.8 | 2.2 | 2.0 | 2.9 | – | 1.2 | 1.3 | 1.4 | 1.8 | 1.3 | 1.3 | 1.5 | 1.2 | 1.9 | 1.4 | 1.3 | 1.5 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.04 | -0.13 | -0.37 | -0.22 | – | -0.63 | -0.56 | -0.72 | -0.54 | -0.13 | -0.14 | -0.15 | 0.02 | 0.08 | -0.05 | -0.32 | -0.16 | -0.15 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
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Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.0 | -0.3 | -0.6 | 1.6 | – | -0.1 | -0.5 | -0.2 | 1.4 | 0.9 | 0.9 | 2.8 | -1.4 | 0 | -1.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 0 |
Endosome | -1.2 | -1.2 | -0.6 | -2.2 | – | 0 | 0 | 0 | 0 | -0.5 | 0.4 | 2.1 | 0.2 | 3.2 | 1.4 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 5.9 | 6.4 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -3.3 | -4.3 | -4.9 | -4.6 | – | -3.6 | -5.1 | -5.7 | -6.2 | -0.6 | -2.3 | -5.0 | -2.9 | -3.6 | -3.0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1.8 | 2.2 | 2.4 | 1.1 | – | 0 | 0 | 0 | 0 | 1.0 | 2.2 | 2.8 | 2.1 | 1.4 | 1.4 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 4.2 | 5.2 | 4.0 | 2.5 | – | 0 | 0 | 0 | 0 | 1.1 | 3.2 | 3.3 | 2.5 | 1.8 | 2.9 |
Vacuole | -0.4 | 1.3 | 0 | -0.1 | – | -0.2 | 1.3 | 1.0 | 1.7 | -0.6 | -0.9 | -0.6 | 1.8 | 1.7 | 2.9 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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