Standard name
Description Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0.05 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.06 0.14 0.18 0.12 0.12 0.2 – 0.14 0.12 0.13 0.19 0.17 0.17 0.25 0.09 0.14 0.1 0 0 0 0.05 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0.05 0.08 0.09 0.05 0.05 0.08 0 – 0 0 0 0.05 0.08 0.11 0.17 0.1 0.22 0.14 0.05 0.06 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.09 0 0 0 0 0.19 0.22 – 0.42 0.47 0.66 0.42 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Nucleus 0.81 0.66 0.65 0.45 0.38 0.38 0.4 – 0.47 0.38 0.36 0.33 0.62 0.53 0.39 0.49 0.46 0.49 0.65 0.55 0.48 0.75 0.65 0.58
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.11 0.15 0.08 0.15 0.16 0.16 0.11 – 0.07 0.06 0 0 0.11 0.15 0.17 0.15 0.12 0.12 0.05 0.05 0.08 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0.11 0.09 0.28 0.34 0.25 0.18 – 0.09 0.07 0.05 0.1 0.13 0.21 0.21 0.18 0.15 0.2 0.11 0.17 0.13 0 0 0.05
Vac/Vac Membrane 0.06 0.13 0.06 0.05 0.1 0.06 0.05 – 0.05 0.09 0.08 0.1 0 0 0 0.11 0.11 0.16 0.08 0.08 0.12 0.12 0.15 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0
Bud 0 1 0 1 1 0 1 – 4 5 8 4 0 0 0 0 0 0 5 7 3 1 3 4
Bud Neck 0 0 0 2 0 1 2 – 1 4 2 0 0 2 0 0 0 0 0 2 1 0 0 1
Bud Site 0 0 0 1 0 5 4 – 5 6 13 9 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0
Cytoplasm 7 20 22 20 14 23 38 – 34 26 35 46 29 42 63 15 31 18 1 7 6 7 7 11
Endoplasmic Reticulum 1 1 0 1 0 0 0 – 0 0 0 0 0 0 1 5 13 3 0 0 1 0 0 0
Endosome 14 28 15 6 6 15 7 – 4 5 4 12 13 26 42 17 49 25 14 23 6 0 3 8
Golgi 0 0 1 0 0 0 0 – 0 0 0 1 0 1 0 0 4 2 0 1 2 0 1 0
Mitochondria 24 7 0 4 5 38 42 – 105 101 177 102 2 2 0 9 10 8 7 4 3 2 8 9
Nucleus 225 219 103 49 43 75 75 – 117 83 98 80 103 129 98 83 105 84 194 206 98 109 145 180
Nuclear Periphery 3 2 0 0 0 0 1 – 0 1 1 0 0 1 0 0 1 0 1 1 2 0 0 0
Nucleolus 31 50 12 16 18 31 21 – 17 14 11 10 18 36 43 25 27 21 15 19 16 1 6 13
Peroxisomes 0 0 1 0 0 3 0 – 0 0 0 0 2 0 3 1 2 2 0 0 0 0 0 0
SpindlePole 11 37 14 31 38 49 34 – 23 16 14 25 21 50 53 31 34 35 31 65 26 2 5 14
Vac/Vac Membrane 16 43 9 5 11 11 10 – 13 20 22 25 7 9 11 19 24 27 23 29 24 17 33 58
Unique Cell Count 278 334 159 110 112 195 188 250 217 269 240 167 242 250 169 226 173 300 376 205 147 222 310
Labelled Cell Count 332 408 177 136 136 251 235 323 281 385 315 195 298 314 206 300 226 300 376 205 147 222 310


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 6.0 6.8 6.6 6.2 5.3 5.8 – 4.4 4.6 4.1 4.7 6.2 6.2 6.1 6.9 7.2 6.6 5.4 6.1 6.1
Std Deviation (1e-4) 0.8 0.9 2.1 2.8 2.2 2.0 2.9 – 1.2 1.3 1.4 1.8 1.3 1.3 1.5 1.2 1.9 1.4 1.3 1.5 1.5
Intensity Change (Log2) – – – -0.04 -0.13 -0.37 -0.22 – -0.63 -0.56 -0.72 -0.54 -0.13 -0.14 -0.15 0.02 0.08 -0.05 -0.32 -0.16 -0.15


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 -0.3 -0.6 1.6 – -0.1 -0.5 -0.2 1.4 0.9 0.9 2.8 -1.4 0 -1.0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 3.1 0
Endosome -1.2 -1.2 -0.6 -2.2 – 0 0 0 0 -0.5 0.4 2.1 0.2 3.2 1.4
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 5.9 6.4 – 0 0 0 0 0 0 0 0 0 0
Nucleus -3.3 -4.3 -4.9 -4.6 – -3.6 -5.1 -5.7 -6.2 -0.6 -2.3 -5.0 -2.9 -3.6 -3.0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.8 2.2 2.4 1.1 – 0 0 0 0 1.0 2.2 2.8 2.1 1.4 1.4
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 4.2 5.2 4.0 2.5 – 0 0 0 0 1.1 3.2 3.3 2.5 1.8 2.9
Vacuole -0.4 1.3 0 -0.1 – -0.2 1.3 1.0 1.7 -0.6 -0.9 -0.6 1.8 1.7 2.9


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Apc5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available