Standard name
Description Essential subunit of Sec63 complex; with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER; other members are Sec62p, Sec66p, and Sec72p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.1 0.09 0.09 0.16 0.11 0.1 0.1 0.07 0.11 0.1 0.13 0.1 0.09 0.1 0.05 0.05 0.07 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.06 0.14 0.1 0.1 0.07 0.07 0.06 0.1 0.1 0.11 0.11 0.18 0.17 0.19 0.36 0.54 0 0 0.05 0 0 0.05
Cytoplasm 0 0.12 0 0.05 0.06 0.06 0 0.06 0.08 0.07 0.1 0.08 0 0 0.08 0.54 0.2 0 0 0 0 0 0
Endoplasmic Reticulum 0.94 0.8 0.9 0.83 0.76 0.82 0.83 0.84 0.75 0.73 0.68 0.67 0.91 0.91 0.85 0 0.05 0.96 0.88 0.9 0.93 0.89 0.88
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.07 0 0.08 0.23 0.23 0 0 0 0 0 0 0 0 0 0 0 0.12 0 0 0 0 0 0
Mitochondria 0 0.05 0 0.11 0.07 0.16 0.13 0.1 0.2 0.22 0.32 0.29 0 0 0 0.1 0.12 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0.06 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 20 26 29 47 50 48 64 62 50 57 58 54 43 45 26 2 3 1 5 8 5 13 13
Bud 0 10 1 2 2 1 6 5 3 3 7 4 1 1 2 0 0 0 3 0 0 1 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cell Periphery 8 17 47 29 44 34 48 55 46 60 46 59 82 82 108 14 22 2 4 15 6 25 34
Cytoplasm 3 33 12 16 25 28 29 50 37 42 45 46 15 15 46 21 8 0 7 0 3 4 3
Endoplasmic Reticulum 184 221 304 246 335 386 549 726 355 426 295 376 423 429 483 1 2 192 256 292 377 515 571
Endosome 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1
Golgi 13 2 27 68 100 21 18 22 10 10 5 6 5 5 1 0 5 1 2 1 2 7 5
Mitochondria 4 14 12 34 30 77 86 89 96 126 138 165 0 0 0 4 5 0 3 1 6 6 7
Nucleus 0 0 1 0 0 0 0 4 0 2 1 0 0 0 0 2 0 0 0 0 0 0 0
Nuclear Periphery 1 0 3 5 4 9 39 34 28 21 13 22 2 2 0 0 1 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 4 5 2 2 23 10 25 35 7 20 10 19 0 2 14 1 2 0 5 0 0 2 5
Unique Cell Count 195 276 336 297 439 469 659 868 471 582 435 560 467 472 569 39 41 200 291 324 406 581 649
Labelled Cell Count 237 328 438 449 613 615 866 1084 632 769 619 752 572 582 680 45 48 200 291 324 406 581 649


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 20.3 15.9 21.3 21.2 18.9 15.5 14.8 14.6 14.0 13.1 12.8 13.1 19.4 19.4 19.6 14.9 18.5 23.2 23.2 23.4
Std Deviation (1e-4) 3.3 3.1 3.9 4.2 3.3 2.9 2.7 2.7 2.6 2.6 2.5 2.8 2.8 2.8 3.3 4.1 4.1 3.7 4.0 4.1
Intensity Change (Log2) -0.01 -0.17 -0.46 -0.53 -0.54 -0.61 -0.7 -0.74 -0.71 -0.14 -0.14 -0.12 -0.52 -0.2 0.12 0.12 0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 2.8 1.3 0.8 0.6 -0.9 0.9 0.6 2.0 0.5 0.3 0.4 -2.5 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.6 -1.7 -3.1 -3.4 -4.3 -1.9 -1.7 -1.4 -1.5 1.4 1.3 1.9 3.5 6.2
Cytoplasm 1.1 1.4 1.5 0.6 1.5 2.5 2.3 3.6 2.7 -0.3 -0.3 2.7 0 0
Endoplasmic Reticulum -2.8 -5.1 -3.3 -3.1 -3.0 -5.5 -6.2 -7.5 -7.9 0 0.2 -2.4 -13.3 -13.2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 5.2 5.5 -2.1 -3.8 -4.3 -4.0 -4.7 -4.8 -5.4 -5.0 -5.0 -6.6 0 0
Mitochondria 3.8 2.0 5.7 4.7 3.8 6.9 7.4 9.8 9.4 -4.1 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 1.2 3.7 2.7 3.7 2.5 2.0 2.7 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 3.6 1.8 2.9 3.1 0 2.7 1.9 2.7 0 0 2.1 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sec63-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available