Standard name | |
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Human Ortholog | |
Description | Essential subunit of Sec63 complex; with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER; other members are Sec62p, Sec66p, and Sec72p |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 20 | 26 | 29 | 47 | 50 | 48 | 64 | 62 | 50 | 57 | 58 | 54 | 43 | 45 | 26 | – | 2 | 3 | 1 | 5 | 8 | 5 | 13 | 13 |
Bud | 0 | 10 | 1 | 2 | 2 | 1 | 6 | 5 | 3 | 3 | 7 | 4 | 1 | 1 | 2 | – | 0 | 0 | 0 | 3 | 0 | 0 | 1 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 8 | 17 | 47 | 29 | 44 | 34 | 48 | 55 | 46 | 60 | 46 | 59 | 82 | 82 | 108 | – | 14 | 22 | 2 | 4 | 15 | 6 | 25 | 34 |
Cytoplasm | 3 | 33 | 12 | 16 | 25 | 28 | 29 | 50 | 37 | 42 | 45 | 46 | 15 | 15 | 46 | – | 21 | 8 | 0 | 7 | 0 | 3 | 4 | 3 |
Endoplasmic Reticulum | 184 | 221 | 304 | 246 | 335 | 386 | 549 | 726 | 355 | 426 | 295 | 376 | 423 | 429 | 483 | – | 1 | 2 | 192 | 256 | 292 | 377 | 515 | 571 |
Endosome | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Golgi | 13 | 2 | 27 | 68 | 100 | 21 | 18 | 22 | 10 | 10 | 5 | 6 | 5 | 5 | 1 | – | 0 | 5 | 1 | 2 | 1 | 2 | 7 | 5 |
Mitochondria | 4 | 14 | 12 | 34 | 30 | 77 | 86 | 89 | 96 | 126 | 138 | 165 | 0 | 0 | 0 | – | 4 | 5 | 0 | 3 | 1 | 6 | 6 | 7 |
Nucleus | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 4 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | – | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 3 | 5 | 4 | 9 | 39 | 34 | 28 | 21 | 13 | 22 | 2 | 2 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 4 | 5 | 2 | 2 | 23 | 10 | 25 | 35 | 7 | 20 | 10 | 19 | 0 | 2 | 14 | – | 1 | 2 | 0 | 5 | 0 | 0 | 2 | 5 |
Unique Cell Count | 195 | 276 | 336 | 297 | 439 | 469 | 659 | 868 | 471 | 582 | 435 | 560 | 467 | 472 | 569 | 39 | 41 | 200 | 291 | 324 | 406 | 581 | 649 | |
Labelled Cell Count | 237 | 328 | 438 | 449 | 613 | 615 | 866 | 1084 | 632 | 769 | 619 | 752 | 572 | 582 | 680 | 45 | 48 | 200 | 291 | 324 | 406 | 581 | 649 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 20.3 | 15.9 | 21.3 | 21.2 | 18.9 | 15.5 | 14.8 | 14.6 | 14.0 | 13.1 | 12.8 | 13.1 | 19.4 | 19.4 | 19.6 | – | 14.9 | 18.5 | 23.2 | 23.2 | 23.4 |
Std Deviation (1e-4) | 3.3 | 3.1 | 3.9 | 4.2 | 3.3 | 2.9 | 2.7 | 2.7 | 2.6 | 2.6 | 2.5 | 2.8 | 2.8 | 2.8 | 3.3 | – | 4.1 | 4.1 | 3.7 | 4.0 | 4.1 |
Intensity Change (Log2) | – | – | – | -0.01 | -0.17 | -0.46 | -0.53 | -0.54 | -0.61 | -0.7 | -0.74 | -0.71 | -0.14 | -0.14 | -0.12 | – | -0.52 | -0.2 | 0.12 | 0.12 | 0.13 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 2.8 | 1.3 | 0.8 | 0.6 | -0.9 | 0.9 | 0.6 | 2.0 | 0.5 | 0.3 | 0.4 | -2.5 | – | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Cell Periphery | -1.6 | -1.7 | -3.1 | -3.4 | -4.3 | -1.9 | -1.7 | -1.4 | -1.5 | 1.4 | 1.3 | 1.9 | – | 3.5 | 6.2 |
Cytoplasm | 1.1 | 1.4 | 1.5 | 0.6 | 1.5 | 2.5 | 2.3 | 3.6 | 2.7 | -0.3 | -0.3 | 2.7 | – | 0 | 0 |
Endoplasmic Reticulum | -2.8 | -5.1 | -3.3 | -3.1 | -3.0 | -5.5 | -6.2 | -7.5 | -7.9 | 0 | 0.2 | -2.4 | – | -13.3 | -13.2 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Golgi | 5.2 | 5.5 | -2.1 | -3.8 | -4.3 | -4.0 | -4.7 | -4.8 | -5.4 | -5.0 | -5.0 | -6.6 | – | 0 | 0 |
Mitochondria | 3.8 | 2.0 | 5.7 | 4.7 | 3.8 | 6.9 | 7.4 | 9.8 | 9.4 | -4.1 | 0 | 0 | – | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Nuclear Periphery | 0 | 0 | 1.2 | 3.7 | 2.7 | 3.7 | 2.5 | 2.0 | 2.7 | 0 | 0 | 0 | – | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Vacuole | 0 | 3.6 | 1.8 | 2.9 | 3.1 | 0 | 2.7 | 1.9 | 2.7 | 0 | 0 | 2.1 | – | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 20.419 | 23.0787 | 22.0012 | 19.0344 | 17.223 | 21.8847 | 19.9389 | 24.7852 | 23.6173 | 22.4001 | 20.197 | 21.7537 | 22.219 | 25.2868 | 22.8653 | 22.4332 | 21.4088 | 23.3429 |
Actin | 0.0164 | 0.0006 | 0.0195 | 0.0367 | 0.0665 | 0.004 | 0.0844 | 0.0003 | 0.0163 | 0.0001 | 0.1502 | 0.0027 | 0.014 | 0.0047 | 0.0075 | 0 | 0.0015 | 0.0034 |
Bud | 0.0004 | 0.0001 | 0.0003 | 0.0006 | 0.001 | 0.0001 | 0.0005 | 0 | 0.0003 | 0 | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0.0004 | 0 | 0.0001 | 0 |
Bud Neck | 0.0029 | 0.0005 | 0.0008 | 0.0002 | 0.0018 | 0.0016 | 0.002 | 0.0003 | 0.001 | 0.0002 | 0.0018 | 0.0015 | 0.001 | 0.0011 | 0.0011 | 0.0001 | 0.0036 | 0.0013 |
Bud Periphery | 0.0007 | 0.0003 | 0.0011 | 0.0014 | 0.0014 | 0.0001 | 0.0007 | 0.0002 | 0.0005 | 0.001 | 0.001 | 0.0004 | 0.0005 | 0.0005 | 0.0011 | 0 | 0.0006 | 0.0001 |
Bud Site | 0.0117 | 0.0002 | 0.0021 | 0.0006 | 0.0084 | 0.0001 | 0.0016 | 0.0001 | 0.0127 | 0 | 0.001 | 0.0016 | 0.0019 | 0.0059 | 0.0079 | 0 | 0.0001 | 0 |
Cell Periphery | 0.0032 | 0.0147 | 0.0101 | 0.0049 | 0.006 | 0.0009 | 0.0064 | 0.0141 | 0.0128 | 0.0047 | 0.0049 | 0.002 | 0.0079 | 0.0267 | 0.013 | 0.007 | 0.0071 | 0.0037 |
Cytoplasm | 0.0089 | 0.0021 | 0.0037 | 0.0023 | 0.0055 | 0.0014 | 0.0055 | 0.0019 | 0.0072 | 0.0005 | 0.001 | 0.0011 | 0.0073 | 0.0081 | 0.0072 | 0.0008 | 0.0409 | 0.0026 |
Cytoplasmic Foci | 0.0113 | 0.0006 | 0.0025 | 0.004 | 0.0151 | 0.0012 | 0.0094 | 0.0003 | 0.0091 | 0 | 0.0014 | 0.0058 | 0.0112 | 0.0006 | 0.0068 | 0 | 0.0021 | 0.0006 |
Eisosomes | 0.0003 | 0.0007 | 0.0004 | 0.0007 | 0.0007 | 0 | 0.001 | 0.0015 | 0.0002 | 0.0001 | 0.0011 | 0.0001 | 0.0006 | 0.001 | 0.0016 | 0.0001 | 0.0001 | 0.0002 |
Endoplasmic Reticulum | 0.8232 | 0.9435 | 0.9119 | 0.8762 | 0.7317 | 0.9669 | 0.7209 | 0.946 | 0.8927 | 0.9775 | 0.8007 | 0.9642 | 0.8462 | 0.9156 | 0.8879 | 0.9845 | 0.851 | 0.9622 |
Endosome | 0.0248 | 0.0085 | 0.0119 | 0.0116 | 0.0284 | 0.0067 | 0.0353 | 0.0021 | 0.0059 | 0.0007 | 0.007 | 0.0027 | 0.0151 | 0.0009 | 0.0091 | 0.0004 | 0.0044 | 0.0011 |
Golgi | 0.0089 | 0.0025 | 0.0064 | 0.0051 | 0.016 | 0.0074 | 0.0122 | 0.0025 | 0.0067 | 0.0006 | 0.0135 | 0.0054 | 0.0102 | 0.0036 | 0.0048 | 0.0011 | 0.0046 | 0.0043 |
Lipid Particles | 0.0134 | 0.0087 | 0.0059 | 0.0054 | 0.0163 | 0.0023 | 0.0195 | 0.0149 | 0.0057 | 0.0001 | 0.0018 | 0.0026 | 0.0068 | 0.0184 | 0.0149 | 0.0003 | 0.0361 | 0.004 |
Mitochondria | 0.0054 | 0.0005 | 0.0058 | 0.0307 | 0.052 | 0.0007 | 0.0034 | 0.0002 | 0.003 | 0.0005 | 0.006 | 0.0006 | 0.0015 | 0.0002 | 0.0041 | 0 | 0.0069 | 0.0002 |
None | 0.0071 | 0.0001 | 0.0001 | 0.0029 | 0.0012 | 0 | 0.0049 | 0.0001 | 0.0033 | 0 | 0.0003 | 0.0001 | 0.0248 | 0.0001 | 0.0132 | 0 | 0.0003 | 0 |
Nuclear Periphery | 0.0356 | 0.0051 | 0.0077 | 0.006 | 0.0046 | 0.0042 | 0.069 | 0.0079 | 0.0124 | 0.0079 | 0.0046 | 0.0049 | 0.0243 | 0.0093 | 0.0104 | 0.0038 | 0.0206 | 0.0065 |
Nucleolus | 0.0017 | 0.0001 | 0 | 0.0001 | 0.0004 | 0 | 0.0003 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0008 | 0 | 0.0001 | 0 | 0.0002 | 0 |
Nucleus | 0.003 | 0.0002 | 0.0003 | 0.0004 | 0.0009 | 0.0002 | 0.0029 | 0.0002 | 0.0007 | 0.0002 | 0.0003 | 0.0002 | 0.0021 | 0.0005 | 0.0005 | 0.0001 | 0.001 | 0.0002 |
Peroxisomes | 0.0046 | 0 | 0.0006 | 0.0012 | 0.0116 | 0.0003 | 0.0026 | 0 | 0.0036 | 0 | 0.0006 | 0.0016 | 0.0084 | 0 | 0.0011 | 0 | 0.0005 | 0.0077 |
Punctate Nuclear | 0.0032 | 0 | 0.0001 | 0.0013 | 0.0034 | 0 | 0.005 | 0 | 0.0017 | 0 | 0.0002 | 0.0002 | 0.0108 | 0.0001 | 0.0001 | 0 | 0.0005 | 0 |
Vacuole | 0.0091 | 0.0089 | 0.0072 | 0.0059 | 0.017 | 0.0014 | 0.0079 | 0.0068 | 0.0034 | 0.0042 | 0.0014 | 0.0019 | 0.0031 | 0.0025 | 0.0057 | 0.0012 | 0.0043 | 0.0012 |
Vacuole Periphery | 0.0041 | 0.0021 | 0.0017 | 0.002 | 0.01 | 0.0004 | 0.0046 | 0.0005 | 0.0006 | 0.0016 | 0.0009 | 0.0004 | 0.0013 | 0.0003 | 0.0015 | 0.0003 | 0.0134 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 112.7223 | 80.7125 | 84.5104 | 90.696 | 91.2087 | 92.4531 | 74.3487 | 82.6202 | 79.1986 | 109.2559 |
Translational Efficiency | 1.2973 | 1.0563 | 1.0647 | 1.0427 | 1.1223 | 1.2564 | 1.0158 | 0.8601 | 1.078 | 0.9839 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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