Standard name
Human Ortholog
Description Essential subunit of Sec63 complex; with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER; other members are Sec62p, Sec66p, and Sec72p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.1 0.09 0.09 0.16 0.11 0.1 0.1 0.07 0.11 0.1 0.13 0.1 0.09 0.1 0.05 0.05 0.07 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.06 0.14 0.1 0.1 0.07 0.07 0.06 0.1 0.1 0.11 0.11 0.18 0.17 0.19 0.36 0.54 0 0 0.05 0 0 0.05
Cytoplasm 0 0.12 0 0.05 0.06 0.06 0 0.06 0.08 0.07 0.1 0.08 0 0 0.08 0.54 0.2 0 0 0 0 0 0
Endoplasmic Reticulum 0.94 0.8 0.9 0.83 0.76 0.82 0.83 0.84 0.75 0.73 0.68 0.67 0.91 0.91 0.85 0 0.05 0.96 0.88 0.9 0.93 0.89 0.88
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.07 0 0.08 0.23 0.23 0 0 0 0 0 0 0 0 0 0 0 0.12 0 0 0 0 0 0
Mitochondria 0 0.05 0 0.11 0.07 0.16 0.13 0.1 0.2 0.22 0.32 0.29 0 0 0 0.1 0.12 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0.06 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 20 26 29 47 50 48 64 62 50 57 58 54 43 45 26 2 3 1 5 8 5 13 13
Bud 0 10 1 2 2 1 6 5 3 3 7 4 1 1 2 0 0 0 3 0 0 1 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cell Periphery 8 17 47 29 44 34 48 55 46 60 46 59 82 82 108 14 22 2 4 15 6 25 34
Cytoplasm 3 33 12 16 25 28 29 50 37 42 45 46 15 15 46 21 8 0 7 0 3 4 3
Endoplasmic Reticulum 184 221 304 246 335 386 549 726 355 426 295 376 423 429 483 1 2 192 256 292 377 515 571
Endosome 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1
Golgi 13 2 27 68 100 21 18 22 10 10 5 6 5 5 1 0 5 1 2 1 2 7 5
Mitochondria 4 14 12 34 30 77 86 89 96 126 138 165 0 0 0 4 5 0 3 1 6 6 7
Nucleus 0 0 1 0 0 0 0 4 0 2 1 0 0 0 0 2 0 0 0 0 0 0 0
Nuclear Periphery 1 0 3 5 4 9 39 34 28 21 13 22 2 2 0 0 1 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 4 5 2 2 23 10 25 35 7 20 10 19 0 2 14 1 2 0 5 0 0 2 5
Unique Cell Count 195 276 336 297 439 469 659 868 471 582 435 560 467 472 569 39 41 200 291 324 406 581 649
Labelled Cell Count 237 328 438 449 613 615 866 1084 632 769 619 752 572 582 680 45 48 200 291 324 406 581 649


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 20.3 15.9 21.3 21.2 18.9 15.5 14.8 14.6 14.0 13.1 12.8 13.1 19.4 19.4 19.6 14.9 18.5 23.2 23.2 23.4
Std Deviation (1e-4) 3.3 3.1 3.9 4.2 3.3 2.9 2.7 2.7 2.6 2.6 2.5 2.8 2.8 2.8 3.3 4.1 4.1 3.7 4.0 4.1
Intensity Change (Log2) -0.01 -0.17 -0.46 -0.53 -0.54 -0.61 -0.7 -0.74 -0.71 -0.14 -0.14 -0.12 -0.52 -0.2 0.12 0.12 0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 2.8 1.3 0.8 0.6 -0.9 0.9 0.6 2.0 0.5 0.3 0.4 -2.5 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.6 -1.7 -3.1 -3.4 -4.3 -1.9 -1.7 -1.4 -1.5 1.4 1.3 1.9 3.5 6.2
Cytoplasm 1.1 1.4 1.5 0.6 1.5 2.5 2.3 3.6 2.7 -0.3 -0.3 2.7 0 0
Endoplasmic Reticulum -2.8 -5.1 -3.3 -3.1 -3.0 -5.5 -6.2 -7.5 -7.9 0 0.2 -2.4 -13.3 -13.2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 5.2 5.5 -2.1 -3.8 -4.3 -4.0 -4.7 -4.8 -5.4 -5.0 -5.0 -6.6 0 0
Mitochondria 3.8 2.0 5.7 4.7 3.8 6.9 7.4 9.8 9.4 -4.1 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 1.2 3.7 2.7 3.7 2.5 2.0 2.7 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 3.6 1.8 2.9 3.1 0 2.7 1.9 2.7 0 0 2.1 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 20.419 23.0787 22.0012 19.0344 17.223 21.8847 19.9389 24.7852 23.6173 22.4001 20.197 21.7537 22.219 25.2868 22.8653 22.4332 21.4088 23.3429
Actin 0.0164 0.0006 0.0195 0.0367 0.0665 0.004 0.0844 0.0003 0.0163 0.0001 0.1502 0.0027 0.014 0.0047 0.0075 0 0.0015 0.0034
Bud 0.0004 0.0001 0.0003 0.0006 0.001 0.0001 0.0005 0 0.0003 0 0.0004 0.0001 0.0003 0.0001 0.0004 0 0.0001 0
Bud Neck 0.0029 0.0005 0.0008 0.0002 0.0018 0.0016 0.002 0.0003 0.001 0.0002 0.0018 0.0015 0.001 0.0011 0.0011 0.0001 0.0036 0.0013
Bud Periphery 0.0007 0.0003 0.0011 0.0014 0.0014 0.0001 0.0007 0.0002 0.0005 0.001 0.001 0.0004 0.0005 0.0005 0.0011 0 0.0006 0.0001
Bud Site 0.0117 0.0002 0.0021 0.0006 0.0084 0.0001 0.0016 0.0001 0.0127 0 0.001 0.0016 0.0019 0.0059 0.0079 0 0.0001 0
Cell Periphery 0.0032 0.0147 0.0101 0.0049 0.006 0.0009 0.0064 0.0141 0.0128 0.0047 0.0049 0.002 0.0079 0.0267 0.013 0.007 0.0071 0.0037
Cytoplasm 0.0089 0.0021 0.0037 0.0023 0.0055 0.0014 0.0055 0.0019 0.0072 0.0005 0.001 0.0011 0.0073 0.0081 0.0072 0.0008 0.0409 0.0026
Cytoplasmic Foci 0.0113 0.0006 0.0025 0.004 0.0151 0.0012 0.0094 0.0003 0.0091 0 0.0014 0.0058 0.0112 0.0006 0.0068 0 0.0021 0.0006
Eisosomes 0.0003 0.0007 0.0004 0.0007 0.0007 0 0.001 0.0015 0.0002 0.0001 0.0011 0.0001 0.0006 0.001 0.0016 0.0001 0.0001 0.0002
Endoplasmic Reticulum 0.8232 0.9435 0.9119 0.8762 0.7317 0.9669 0.7209 0.946 0.8927 0.9775 0.8007 0.9642 0.8462 0.9156 0.8879 0.9845 0.851 0.9622
Endosome 0.0248 0.0085 0.0119 0.0116 0.0284 0.0067 0.0353 0.0021 0.0059 0.0007 0.007 0.0027 0.0151 0.0009 0.0091 0.0004 0.0044 0.0011
Golgi 0.0089 0.0025 0.0064 0.0051 0.016 0.0074 0.0122 0.0025 0.0067 0.0006 0.0135 0.0054 0.0102 0.0036 0.0048 0.0011 0.0046 0.0043
Lipid Particles 0.0134 0.0087 0.0059 0.0054 0.0163 0.0023 0.0195 0.0149 0.0057 0.0001 0.0018 0.0026 0.0068 0.0184 0.0149 0.0003 0.0361 0.004
Mitochondria 0.0054 0.0005 0.0058 0.0307 0.052 0.0007 0.0034 0.0002 0.003 0.0005 0.006 0.0006 0.0015 0.0002 0.0041 0 0.0069 0.0002
None 0.0071 0.0001 0.0001 0.0029 0.0012 0 0.0049 0.0001 0.0033 0 0.0003 0.0001 0.0248 0.0001 0.0132 0 0.0003 0
Nuclear Periphery 0.0356 0.0051 0.0077 0.006 0.0046 0.0042 0.069 0.0079 0.0124 0.0079 0.0046 0.0049 0.0243 0.0093 0.0104 0.0038 0.0206 0.0065
Nucleolus 0.0017 0.0001 0 0.0001 0.0004 0 0.0003 0.0001 0.0002 0 0.0001 0 0.0008 0 0.0001 0 0.0002 0
Nucleus 0.003 0.0002 0.0003 0.0004 0.0009 0.0002 0.0029 0.0002 0.0007 0.0002 0.0003 0.0002 0.0021 0.0005 0.0005 0.0001 0.001 0.0002
Peroxisomes 0.0046 0 0.0006 0.0012 0.0116 0.0003 0.0026 0 0.0036 0 0.0006 0.0016 0.0084 0 0.0011 0 0.0005 0.0077
Punctate Nuclear 0.0032 0 0.0001 0.0013 0.0034 0 0.005 0 0.0017 0 0.0002 0.0002 0.0108 0.0001 0.0001 0 0.0005 0
Vacuole 0.0091 0.0089 0.0072 0.0059 0.017 0.0014 0.0079 0.0068 0.0034 0.0042 0.0014 0.0019 0.0031 0.0025 0.0057 0.0012 0.0043 0.0012
Vacuole Periphery 0.0041 0.0021 0.0017 0.002 0.01 0.0004 0.0046 0.0005 0.0006 0.0016 0.0009 0.0004 0.0013 0.0003 0.0015 0.0003 0.0134 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 112.7223 80.7125 84.5104 90.696 91.2087 92.4531 74.3487 82.6202 79.1986 109.2559
Translational Efficiency 1.2973 1.0563 1.0647 1.0427 1.1223 1.2564 1.0158 0.8601 1.078 0.9839

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Essential subunit of Sec63 complex; with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER; other members are Sec62p, Sec66p, and Sec72p
Localization
Cell Percentages ER (98%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Sec63

Sec63


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sec63-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available