Standard name
Human Ortholog
Description ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.12 0 0.07 0.14 0 0.13 0.05 0.05 0.05 0.09 0 0.37 0.17 0.12 0.19 0 0 0 0 0 0.11
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.06 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0.05 0 0 0.07 0 0 0 0 0 0 0 0 0 0
Nucleus 0.75 0.9 0.78 0.81 0.83 0.65 0.72 0.59 0.69 0.65 0.56 0.53 0.71 0.71 0.59 0.75 0.91 0.81 0.72 0.83 0.71
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.12 0 0.15 0.06 0.13 0.09 0.07 0.2 0.18 0.17 0.33 0 0 0.06 0.05 0.1 0 0.06 0 0.07 0.09
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.19 0.06 0 0 0.05 0.15 0.21 0.17 0.11 0 0.13 0.05 0.08 0.1 0.17 0.05 0 0.06 0.28 0.05 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 2 1 0 2 0 0 0 0 0 0 0 0 3 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 1 1 0 2 0 0 0 0 0
Cell Periphery 0 0 0 0 0 1 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 2 2 2 5 3 22 9 4 6 2 2 7 45 39 31 1 0 0 0 0 3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 5 9 4 0 0 1 0 0 0
Endosome 0 0 0 0 0 0 0 1 0 0 0 0 1 4 1 1 0 0 0 0 0
Golgi 0 0 0 0 0 0 1 0 0 0 0 1 3 5 10 0 0 0 0 0 0
Mitochondria 0 0 0 0 1 1 0 4 3 1 4 0 8 2 2 0 0 0 0 0 0
Nucleus 12 47 21 29 64 106 118 44 90 15 30 10 186 232 96 30 13 69 1 20 24
Nuclear Periphery 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 2 2 4 2 10 14 11 15 23 4 18 0 5 21 8 3 0 5 0 1 3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 3 3 0 1 4 24 34 13 15 1 7 1 22 32 27 1 0 5 0 1 1
Unique Cell Count 16 52 27 36 77 163 164 75 131 23 54 19 261 325 162 40 15 85 2 25 35
Labelled Cell Count 19 54 28 37 82 170 176 83 139 26 61 19 277 344 179 40 15 85 2 25 35


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 19.3 27.4 25.4 26.4 23.1 23.4 24.1 21.7 21.8 21.9 19.1 26.1 33.5 32.9 33.8
Std Deviation (1e-4) 10.3 7.8 6.1 7.5 5.7 7.2 7.2 6.4 6.7 7.6 6.6 11.9 15.1 14.0 15.8
Intensity Change (Log2) -0.11 -0.05 -0.24 -0.23 -0.18 -0.34 -0.33 -0.32 -0.52 -0.07 0.29 0.27 0.3


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 1.9 0 0 0 0 0 0 2.5 1.8 2.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus -1.5 -1.3 -1.2 -3.5 -2.7 -3.9 -3.0 -2.7 -4.0 -3.5 -2.9 -2.9 -4.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 2.6 2.4 0 3.9 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 1.7 2.5 1.9 1.2 0 0 0 0 0 2.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin
Bud
Bud Neck
Bud Periphery
Bud Site
Cell Periphery
Cytoplasm
Cytoplasmic Foci
Eisosomes
Endoplasmic Reticulum
Endosome
Golgi
Lipid Particles
Mitochondria
None
Nuclear Periphery
Nucleolus
Nucleus
Peroxisomes
Punctate Nuclear
Vacuole
Vacuole Periphery

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 91.9504 86.3267 97.4798 115.8793 91.4547 88.1228 102.4648 122.1771 125.8127 96.8333
Translational Efficiency 2.257 1.9746 2.0216 1.7808 1.8324 2.4987 1.9521 1.559 1.5909 1.7786

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
33 123 1588 747 1079 97 1214 180 1112 220 2802 927

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1354.09 2333.90 2229.75 2215.34 1838.27 2120.12 2448.45 2356.11 1823.90 2239.64 2324.50 2242.67
Standard Deviation 623.90 558.97 528.92 604.15 461.29 443.88 554.97 673.87 474.11 522.33 551.12 620.81
Intensity Change Log 2 0.785419 0.719558 0.710205 0.205797 0.413520 0.358058 0.480485 0.551330 0.518028

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000940 0.000640 0.000599 0.003866 0.000634 0.001554 0.000743 0.007169 0.000643 0.001043 0.000661 0.004508
Bud Neck 0.004746 0.004810 0.017594 0.030999 0.004064 0.014190 0.018679 0.027072 0.004084 0.008946 0.018064 0.030237
Bud Site 0.002487 0.000549 0.001441 0.007926 0.000237 0.002565 0.002030 0.014555 0.000303 0.001438 0.001697 0.009213
Cell Periphery 0.000690 0.000043 0.000116 0.001201 0.000090 0.000213 0.000184 0.001940 0.000108 0.000118 0.000145 0.001344
Cytoplasm 0.171273* 0.000441 0.001029 0.004959 0.000349 0.000480 0.002540 0.004388 0.005422 0.000458 0.001684 0.004848
Cytoplasmic Foci 0.015733 0.003253 0.001058 0.003875 0.000089 0.000254 0.002054 0.006443 0.000553 0.001931 0.001489 0.004374
Eisosomes 0.000059 0.000043 0.000046 0.000039 0.000053 0.000051 0.000046 0.000334 0.000053 0.000047 0.000046 0.000096
Endoplasmic Reticulum 0.026166 0.000498 0.000762 0.001354 0.000762 0.002599 0.000668 0.002664 0.001516 0.001424 0.000721 0.001609
Endosome 0.006345 0.000565 0.001494 0.008434 0.000018 0.000627 0.001222 0.005833 0.000206 0.000592 0.001377 0.007929
Golgi 0.001759 0.001935 0.000543 0.009095 0.000032 0.000302 0.001449 0.006565 0.000083 0.001215 0.000935 0.008604
Lipid Particles 0.008036 0.000118 0.000105 0.000394 0.000008 0.000426 0.000187 0.004587 0.000247 0.000254 0.000141 0.001208
Mitochondria 0.005332 0.001395 0.002353 0.008313 0.001424 0.002248 0.003821 0.004829 0.001540 0.001771 0.002989 0.007636
Mitotic Spindle 0.000591 0.000998 0.002818 0.031248 0.000094 0.052299 0.002284 0.014539 0.000109 0.023617 0.002587 0.028004
None 0.005747 0.000616 0.001426 0.006054 0.002416 0.001093 0.002131 0.003376 0.002515 0.000827 0.001732 0.005534
Nuclear Periphery 0.008251 0.000534 0.000319 0.004709 0.000144 0.001249 0.000734 0.002129 0.000384 0.000849 0.000499 0.004208
Nuclear Periphery Foci 0.028881 0.000317 0.000184 0.002389 0.000029 0.002832 0.000439 0.003489 0.000885 0.001426 0.000294 0.002603
Nucleolus 0.037718 0.015264 0.008879 0.028820 0.023434 0.022397 0.013185 0.020085 0.023858 0.018409 0.010745 0.027124
Nucleus 0.537921* 0.965742* 0.957740* 0.831099* 0.964611* 0.889771* 0.945290* 0.859469* 0.951949* 0.932246* 0.952346* 0.836608*
Peroxisomes 0.003382 0.001134 0.000418 0.001276 0.000896 0.001355 0.000569 0.001273 0.000970 0.001232 0.000484 0.001276
Vacuole 0.121153 0.000867 0.000660 0.008740 0.000402 0.002931 0.001106 0.008462 0.003985 0.001777 0.000853 0.008686
Vacuole Periphery 0.012791 0.000236 0.000416 0.005207 0.000214 0.000564 0.000639 0.000797 0.000587 0.000381 0.000513 0.004351

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.54 1.45 -1.48 -1.72 -1.68 -2.21 -4.35 -1.43 -0.87 -1.36 -2.14 -3.33 -2.18 -1.58 -2.09
Bud Neck -0.10 -8.58 -11.61 -12.99 -6.65 -3.34 -16.51 -8.13 -3.71 -3.29 -3.46 -21.33 -15.62 -10.47 -7.36
Bud Site 2.80 1.05 -3.55 -5.14 -4.31 -2.16 -5.97 -2.99 -1.82 -2.40 -2.25 -8.30 -6.18 -4.27 -4.84
Cell Periphery 1.72 1.51 -0.33 -1.92 -1.74 -1.39 -2.17 -1.32 -1.14 -1.19 -0.36 -1.31 -2.14 -2.08 -2.03
Cytoplasm 3.43 3.41 3.33 -6.03 -4.78 -1.11 -3.08 -4.73 -4.34 -1.46 2.86 2.00 0.15 -6.95 -4.25
Cytoplasmic Foci 1.81 2.54 2.14 0.09 -2.41 -2.01 -3.85 -1.86 -1.72 -0.95 -0.81 -2.40 -3.37 -0.58 -2.29
Eisosomes 1.04 0.69 1.28 0.56 2.19 -0.21 0.61 -0.97 -0.96 -0.99 0.82 0.75 -0.57 -0.75 -0.71
Endoplasmic Reticulum 3.78 3.74 3.67 -2.94 -1.99 -1.61 0.83 -1.55 0.80 -1.65 -0.40 3.11 0.28 0.52 -2.70
Endosome 3.03 2.75 0.62 -2.96 -2.61 -2.02 -3.05 -1.82 -1.33 -1.27 -1.35 -2.55 -3.86 -3.26 -2.98
Golgi -0.15 1.63 -1.83 -1.23 -2.64 -2.45 -1.95 -1.20 -1.05 -0.81 -1.12 -2.42 -3.08 -1.89 -2.63
Lipid Particles 2.01 2.02 1.96 -1.37 -2.30 -1.85 -2.40 -1.38 -1.07 -1.29 -0.46 0.88 -1.35 -1.09 -1.69
Mitochondria 1.26 0.86 -0.99 -6.56 -5.36 -2.43 -3.76 -7.43 -4.73 -2.44 -1.52 -4.86 -7.51 -6.89 -5.26
Mitotic Spindle -0.77 -2.28 -6.58 -6.22 -6.10 -2.95 -2.58 -3.08 1.49 -2.80 -2.96 -3.85 -7.35 -0.40 -6.73
None 2.25 1.77 -0.03 -3.96 -3.12 1.29 0.40 -1.61 -4.55 -3.32 1.73 0.73 -1.98 -4.41 -3.44
Nuclear Periphery 2.15 2.39 0.69 -4.30 -7.03 -2.05 -3.38 -3.47 -1.84 -2.68 -1.44 -1.66 -7.61 -4.94 -7.25
Nuclear Periphery Foci 2.39 2.42 2.25 -2.93 -4.53 -1.31 -2.50 -1.62 0.11 -1.41 -0.81 1.52 -2.30 -0.27 -3.83
Nucleolus 1.16 1.40 0.05 -3.68 -6.93 -0.27 2.63 -0.85 -0.32 -2.04 0.85 4.33 -2.91 -2.82 -6.83
Nucleus -6.68 -6.54 -4.53 9.95 12.22 3.41 6.14 6.36 0.65 4.85 2.11 1.64 12.92 5.52 12.81
Peroxisomes 0.84 1.10 0.78 -0.34 -3.67 -1.55 1.72 -1.47 0.81 -2.17 -1.23 3.20 -1.61 0.34 -3.88
Vacuole 4.05 4.05 3.87 -3.87 -4.35 -1.83 -2.96 -2.10 -0.69 -1.69 1.49 2.78 -1.23 -2.87 -4.48
Vacuole Periphery 2.76 2.74 1.98 -2.82 -2.72 -1.84 -1.50 -3.25 0.05 -0.43 0.74 0.74 -2.53 -2.69 -2.69
Standard name
Human Ortholog
Description ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization
Localization
Cell Percentages nucleus (95%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-5

Rpt4

Rpt4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpt4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available