Standard name
Description Gamma subunit of the translation initiation factor eIF2B; the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0.05 0.06 0.06 0.06 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0.05 0.05 0.05 0.06 0.11 0.12 0.11 0.1 0.14 0 0 0 0.06 0.05 0.09 0 0 0 0 0 0
Cytoplasm 0.97 0.94 0.84 0.89 0.74 0.63 0.73 0.7 0.62 0.61 0.58 0.57 0.77 0.82 0.85 0.93 0.92 0.92 0.94 0.93 0.79 0.69 0.74 0.77
Endoplasmic Reticulum 0 0.06 0.12 0.08 0.07 0.17 0.18 0.09 0.12 0.14 0.12 0.11 0.19 0.12 0.1 0.08 0 0.07 0 0 0.13 0.18 0.16 0.15
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.07 0.23 0.05 0 0.18 0.15 0.31 0.22 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.11 0 0.06 0.11 0.09 0.07 0 0.1 0.06 0.07 0 0 0 0 0 0 0 0.06 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 5 13 7 11 25 20 47 77 71 80 96 2 0 1 0 1 0 0 4 4 1 0 1
Bud 0 6 2 0 0 0 0 2 7 3 18 3 0 0 0 0 0 0 1 3 4 1 3 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 1 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 3 24 40 46 50 64 65 108 160 123 134 155 2 3 13 6 4 7 0 0 11 0 0 0
Cytoplasm 164 942 1098 786 773 789 783 718 814 685 751 626 168 305 274 101 70 70 163 956 925 204 369 303
Endoplasmic Reticulum 4 59 157 67 70 214 191 95 161 160 156 120 41 43 33 9 2 5 1 27 156 52 78 57
Endosome 0 0 1 0 3 0 0 0 3 2 3 0 1 1 0 0 0 0 0 1 4 1 1 0
Golgi 0 2 48 9 33 4 23 52 34 30 12 32 5 7 5 1 1 1 1 2 22 4 7 5
Mitochondria 1 2 8 9 77 289 51 26 242 169 402 247 0 0 1 1 0 1 0 2 12 7 4 5
Nucleus 1 0 1 0 3 3 7 6 1 2 3 2 0 1 2 0 1 0 1 2 0 0 0 0
Nuclear Periphery 0 0 0 1 2 16 1 1 1 12 3 14 0 0 0 0 0 0 0 1 4 0 2 0
Nucleolus 0 0 0 0 0 1 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0
Vac/Vac Membrane 0 4 30 24 114 38 59 111 117 78 56 116 13 26 13 4 2 2 3 16 13 18 24 14
Unique Cell Count 169 1003 1304 887 1043 1258 1068 1028 1317 1119 1303 1107 218 370 324 109 76 76 173 1028 1172 297 500 396
Labelled Cell Count 173 1044 1398 949 1137 1443 1201 1166 1618 1336 1619 1411 232 386 342 122 81 86 173 1028 1172 297 500 396


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 20.5 20.8 18.7 16.3 13.8 11.4 12.9 15.5 12.0 12.3 11.0 11.5 19.0 19.8 19.5 20.8 19.9 20.2 17.4 17.9 19.1
Std Deviation (1e-4) 3.0 3.4 3.3 2.6 2.6 2.6 2.9 2.5 2.6 2.4 2.3 2.3 3.6 3.2 3.7 6.3 6.2 6.3 3.3 3.5 3.8
Intensity Change (Log2) -0.2 -0.45 -0.72 -0.54 -0.28 -0.64 -0.61 -0.76 -0.7 0.02 0.08 0.06 0.15 0.09 0.11 -0.11 -0.06 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.5 0.1 2.1 1.8 5.4 6.8 7.2 7.1 9.0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 3.6 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2.5 2.2 2.6 3.6 7.3 8.8 7.8 7.4 9.8 -1.8 -2.4 0.9 0 0 0
Cytoplasm 2.9 -6.0 -12.3 -6.5 -8.3 -12.9 -12.8 -14.9 -15.0 -2.6 -0.8 0.2 2.4 1.9 1.9
Endoplasmic Reticulum -3.4 -4.3 3.6 4.0 -2.2 0.1 1.6 -0.1 -0.9 2.7 -0.2 -0.9 -1.2 -2.5 -1.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi -3.8 -0.7 -6.0 -2.2 1.6 -1.6 -1.4 -4.7 -1.1 -1.0 -1.7 -1.9 0 0 0
Mitochondria 1.1 8.7 17.7 6.5 3.8 15.5 13.7 21.2 17.3 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 4.1 0 0 0 3.7 0 4.1 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.6 8.7 1.1 4.1 8.5 7.3 5.6 2.9 8.4 3.0 4.5 1.7 0 0 0