Standard name
Human Ortholog
Description Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.07 0.08 0.06 0 0 0 0 0 0
Cytoplasm 0.99 0.97 0.98 0.97 0.96 0.85 0.75 0.8 0.69 0.69 0.65 0.69 0.99 1.0 1.0 0.94 0.96 0.96 0.94 0.96 0.92 0.9 0.94 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.21 0.32 0.25 0.44 0.47 0.47 0.36 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 1 0 1 0 2 4 0 0 1 0 0 0 0 0 0 0 0 0
Bud 0 1 0 1 1 0 3 6 4 8 10 4 0 1 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 4 5 10 4 4 6 5 2 2 7 7 2 8 8 21 21 16 0 0 0 1 0 0
Cytoplasm 302 168 139 192 270 167 208 246 160 158 116 153 193 238 211 289 241 268 294 167 157 136 298 281
Endoplasmic Reticulum 2 5 3 1 5 2 2 4 0 0 1 8 1 0 0 13 10 11 1 2 1 4 7 8
Endosome 0 0 1 0 0 1 0 2 0 0 1 0 0 0 0 1 0 0 2 1 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 1 0 0 1 0 1
Mitochondria 5 1 4 7 7 42 89 78 101 108 85 81 0 0 0 5 2 4 9 0 4 4 5 4
Nucleus 0 0 0 2 0 1 3 1 2 1 1 1 0 0 0 3 2 0 0 0 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 0 0 2 0 0 0 1 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 1 1 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 0 0 0 2 2 6 10 6 5 7 5 6 0 0 0 14 1 3 0 1 1 0 0 1
Unique Cell Count 305 174 142 198 281 196 277 307 232 230 179 223 195 239 212 307 252 279 312 175 172 152 316 304
Labelled Cell Count 313 179 152 215 291 224 323 349 276 287 229 265 198 247 221 346 279 303 312 175 172 152 316 304


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.5 8.3 7.8 7.4 7.2 5.7 5.6 5.8 4.9 5.3 5.0 5.1 8.2 8.2 8.3 14.7 16.5 16.4 7.4 8.1 8.5
Std Deviation (1e-4) 0.8 1.0 0.9 1.2 1.3 0.9 0.9 1.0 0.9 1.0 1.2 0.8 1.2 1.2 1.2 3.1 3.2 4.2 1.4 1.3 1.6
Intensity Change (Log2) – – – -0.08 -0.11 -0.44 -0.48 -0.43 -0.66 -0.54 -0.63 -0.62 0.08 0.08 0.1 0.91 1.08 1.07 -0.07 0.05 0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.7 0 0 0 0 0 0 0.2 0 0 0 0.1 1.4 1.8 1.0
Cytoplasm -0.5 -1.0 -3.9 -5.9 -5.0 -6.8 -6.8 -7.3 -6.8 0.8 1.6 1.4 -1.8 -1.2 -1.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 1.1 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.9 6.8 5.8 8.5 9.0 8.9 7.4 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.3428 5.9182 5.1778 4.6271 4.4023 5.199 3.5685 5.4853 4.6189 4.0131 3.8099 4.6491 3.7143 5.2138 3.9367 4.0864 3.8067 4.2833
Actin 0.0065 0.0001 0.002 0.0014 0.0032 0.0006 0.0015 0.0003 0.0037 0.0002 0.0007 0.0004 0.0304 0.0006 0.0168 0.0001 0.0007 0.0103
Bud 0.0005 0.0002 0.0048 0.0009 0.0007 0.0003 0.001 0.0004 0.0005 0.0003 0.0002 0.0002 0.0007 0.0005 0.0006 0.0003 0.003 0.0003
Bud Neck 0.0049 0.0001 0.0008 0.0023 0.0007 0.0009 0.0004 0.0002 0.0008 0.0005 0.0005 0.0007 0.0027 0.0003 0.0004 0.0004 0.002 0.0006
Bud Periphery 0.0005 0 0.0022 0.001 0.0004 0.0002 0.0006 0.0001 0.0003 0.0001 0.0001 0.0003 0.0012 0.0001 0.0005 0.0001 0.0016 0.0002
Bud Site 0.0034 0.0005 0.0082 0.0008 0.0012 0.0004 0.0024 0.0018 0.0007 0.001 0.0002 0.0001 0.0046 0.0026 0.0035 0.0002 0.0238 0.0001
Cell Periphery 0.0004 0 0.0003 0.0002 0.0001 0 0.0003 0.0001 0.0001 0 0 0.0001 0.0006 0.0002 0.0002 0.0001 0.0002 0
Cytoplasm 0.7799 0.9525 0.8953 0.7868 0.7523 0.916 0.8866 0.9453 0.907 0.9267 0.9324 0.9075 0.7573 0.9493 0.8659 0.9517 0.8469 0.8795
Cytoplasmic Foci 0.0482 0.0129 0.0221 0.0327 0.0698 0.0206 0.0185 0.0114 0.0155 0.0157 0.0199 0.0211 0.0313 0.0073 0.0204 0.0109 0.0274 0.018
Eisosomes 0.0001 0 0 0 0 0 0 0 0 0 0 0 0.0009 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0075 0.0009 0.0035 0.0067 0.0024 0.0056 0.016 0.0061 0.0098 0.0042 0.0082 0.0074 0.0282 0.0053 0.0068 0.0048 0.0031 0.0134
Endosome 0.0542 0.0192 0.0295 0.1112 0.0712 0.0289 0.0286 0.0181 0.0303 0.0333 0.0281 0.0327 0.0522 0.0081 0.0443 0.0206 0.0439 0.0529
Golgi 0.0121 0.0013 0.0052 0.0062 0.0301 0.0026 0.0024 0.0012 0.0019 0.001 0.0031 0.0031 0.0133 0.001 0.0061 0.0006 0.0071 0.0097
Lipid Particles 0.0088 0 0.0009 0.0003 0.0053 0.0008 0.0014 0.0001 0.0005 0.0001 0 0.0005 0.0087 0.0001 0.0038 0.0001 0.0175 0.0004
Mitochondria 0.0107 0.0003 0.0019 0.006 0.0354 0.0031 0.0013 0.0005 0.0007 0.0006 0.0009 0.0068 0.008 0.0004 0.0097 0.0002 0.0041 0.0076
None 0.0396 0.0078 0.0119 0.0097 0.0057 0.0137 0.027 0.0094 0.0197 0.0036 0.0012 0.0124 0.0096 0.0174 0.0086 0.0011 0.0018 0.0009
Nuclear Periphery 0.0024 0.0002 0.0005 0.0013 0.0006 0.0003 0.0027 0.0005 0.001 0.001 0.0003 0.0005 0.0062 0.0007 0.0013 0.0007 0.0012 0.0006
Nucleolus 0.0003 0 0.0001 0.0001 0.0001 0 0.0001 0 0.0001 0 0 0.0001 0.0005 0.0001 0.0001 0 0.0006 0
Nucleus 0.0016 0.0006 0.0013 0.0015 0.0011 0.0007 0.001 0.0007 0.0009 0.0014 0.0007 0.0007 0.0268 0.0037 0.0011 0.0007 0.0019 0.0006
Peroxisomes 0.0064 0.0001 0.0031 0.0003 0.011 0.0014 0.0006 0 0.0003 0.0001 0.0001 0.0003 0.0058 0.0001 0.0018 0 0.0026 0.0004
Punctate Nuclear 0.0045 0.0001 0.0003 0.0003 0.0009 0.0005 0.001 0.0001 0.0008 0.0004 0.0001 0.0018 0.0039 0.0009 0.0004 0.0001 0.0018 0.0001
Vacuole 0.0062 0.0025 0.0051 0.0249 0.0055 0.0025 0.0056 0.0031 0.0046 0.0082 0.0024 0.0026 0.0058 0.0014 0.006 0.0062 0.0071 0.003
Vacuole Periphery 0.0014 0.0005 0.0009 0.0055 0.0024 0.0008 0.0009 0.0005 0.001 0.0015 0.0008 0.0008 0.0014 0.0003 0.0016 0.001 0.0016 0.0014

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 26.951 20.7857 26.6413 29.0557 20.5548 12.6758 26.987 29.9812 28.1026 23.9932
Translational Efficiency 1.1181 1.4427 1.2915 1.1135 1.0929 1.9977 1.1313 0.8438 0.814 0.9789

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1368 939 2839 1655 1256 1753 369 1209 2624 2692 3208 2864

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 789.07 931.63 1114.51 1119.39 860.31 923.41 1194.34 1113.87 823.17 926.28 1123.69 1117.06
Standard Deviation 126.06 183.15 139.17 179.99 109.73 114.01 142.74 162.50 123.75 142.06 141.89 172.85
Intensity Change Log 2 — 0.239604 0.498184 0.504488 — 0.102115 0.473285 0.372652 — 0.169526 0.485251 0.437227

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000166 0.000263 0.000252 0.000367 0.000162 0.000158 0.000321 0.000606 0.000164 0.000195 0.000260 0.000468
Bud Neck 0.006179 0.003027 0.000782 0.001854 0.003234 0.005608 0.001351 0.002675 0.004770 0.004708 0.000848 0.002201
Bud Site 0.025281 0.028903 0.009647 0.017569 0.013865 0.016381 0.017343 0.019323 0.019817 0.020748 0.010532 0.018309
Cell Periphery 0.000175 0.000225 0.000046 0.000108 0.000183 0.000095 0.000087 0.000065 0.000179 0.000140 0.000050 0.000090
Cytoplasm 0.605372* 0.681230* 0.880524* 0.692635* 0.626463* 0.528573* 0.698700* 0.584485* 0.615467* 0.581821* 0.859610* 0.646981*
Cytoplasmic Foci 0.223282* 0.183939* 0.021539 0.038282 0.228685* 0.146916 0.089571 0.053354 0.225868* 0.159830 0.029364 0.044644
Eisosomes 0.000064 0.000028 0.000003 0.000009 0.000061 0.000028 0.000018 0.000011 0.000063 0.000028 0.000005 0.000010
Endoplasmic Reticulum 0.002322 0.002047 0.000112 0.000630 0.005216 0.002454 0.000478 0.000404 0.003708 0.002312 0.000154 0.000535
Endosome 0.028377 0.014895 0.001361 0.009221 0.018847 0.030816 0.007284 0.014432 0.023815 0.025263 0.002042 0.011421
Golgi 0.012147 0.008035 0.000720 0.002858 0.011248 0.006144 0.000419 0.005702 0.011716 0.006804 0.000685 0.004059
Lipid Particles 0.002913 0.001461 0.000397 0.002740 0.003138 0.002051 0.000816 0.000732 0.003021 0.001845 0.000446 0.001893
Mitochondria 0.002279 0.000735 0.000128 0.002173 0.002427 0.001494 0.000016 0.001483 0.002350 0.001229 0.000115 0.001882
Mitotic Spindle 0.001025 0.000817 0.001677 0.007718 0.000900 0.004085 0.001167 0.006270 0.000965 0.002945 0.001618 0.007107
None 0.003238 0.011920 0.000441 0.001277 0.001065 0.001367 0.000717 0.001004 0.002198 0.005048 0.000473 0.001162
Nuclear Periphery 0.000163 0.000051 0.000029 0.000210 0.000061 0.000134 0.000108 0.000077 0.000114 0.000105 0.000038 0.000154
Nuclear Periphery Foci 0.000784 0.000731 0.000361 0.001188 0.000489 0.000354 0.005533 0.000745 0.000643 0.000486 0.000956 0.001001
Nucleolus 0.000469 0.000636 0.000114 0.000246 0.000150 0.000281 0.000078 0.000302 0.000317 0.000405 0.000110 0.000270
Nucleus 0.010763 0.010357 0.011107 0.008350 0.009736 0.014818 0.014723 0.011010 0.010271 0.013262 0.011523 0.009473
Peroxisomes 0.001440 0.001057 0.000319 0.001870 0.000985 0.001569 0.000551 0.001616 0.001222 0.001391 0.000346 0.001763
Vacuole 0.067318 0.045100 0.069871 0.205983* 0.066861 0.227814* 0.160390 0.293197* 0.067099 0.164081 0.080283 0.242799*
Vacuole Periphery 0.006243 0.004541 0.000571 0.004711 0.006226 0.008861 0.000329 0.002507 0.006234 0.007354 0.000543 0.003781

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.23 -0.71 -3.22 -1.01 -0.75 0.19 -4.82 -1.16 -1.17 -0.56 -1.73 -0.95 -1.84 -1.59 -0.96
Bud Neck 5.25 12.59 9.96 2.82 -7.74 -4.87 6.72 1.99 6.35 -4.29 0.16 15.14 9.45 7.77 -9.45
Bud Site -1.53 10.33 5.21 5.80 -4.89 -1.78 -1.86 -1.77 -0.56 -0.28 -0.73 9.18 2.52 3.13 -5.50
Cell Periphery -1.33 12.76 6.93 3.53 -6.49 7.84 8.18 11.60 5.11 4.40 2.58 17.01 12.37 4.42 -5.28
Cytoplasm -7.00 -34.52 -8.01 -0.34 23.08 9.79 -2.71 4.38 -4.40 5.71 4.50 -38.35 -3.24 -7.27 31.18
Cytoplasmic Foci 5.11 39.84 35.72 24.14 -7.49 12.90 20.57 31.56 22.96 6.49 13.87 52.47 47.86 34.42 -7.58
Eisosomes 4.96 12.01 10.90 3.90 -6.73 8.17 10.37 12.79 11.24 5.75 9.21 17.78 16.38 9.26 -6.29
Endoplasmic Reticulum 0.51 6.20 4.85 3.69 -3.17 2.57 4.71 4.81 5.51 1.63 2.37 6.84 6.24 6.39 -3.79
Endosome 7.31 17.56 12.50 6.08 -9.30 -7.36 10.87 4.63 11.12 -4.48 -1.08 23.21 12.94 14.46 -12.02
Golgi 5.15 17.49 12.94 8.64 -3.08 6.70 22.68 6.17 1.52 -3.91 8.46 25.31 13.48 5.62 -4.64
Lipid Particles 4.15 8.15 0.92 -1.31 -3.07 2.75 9.73 10.74 3.83 1.83 4.01 13.29 3.45 0.48 -3.28
Mitochondria 2.43 3.63 0.80 -1.43 -2.61 1.25 3.53 1.60 0.67 -2.52 2.25 4.98 1.59 -0.42 -3.42
Mitotic Spindle 0.28 -0.82 -3.44 -3.84 -3.22 -2.79 -1.06 -3.02 -0.44 -2.67 -2.43 -1.28 -4.55 -2.32 -3.95
None -2.90 3.14 2.13 3.75 -1.01 -0.49 2.03 0.59 0.79 -0.29 -2.44 3.67 2.14 3.65 -1.23
Nuclear Periphery 1.56 1.71 -0.14 -1.43 -1.54 -2.84 -7.24 -2.51 1.92 5.45 0.22 1.60 -0.40 -0.63 -1.64
Nuclear Periphery Foci 0.65 2.48 -0.75 -1.25 -2.37 0.94 -9.27 -2.45 -2.72 8.64 1.50 -2.77 -1.49 -2.53 0.25
Nucleolus -0.99 4.56 2.81 2.78 -4.16 -3.91 4.95 -2.36 0.08 -3.60 -1.24 4.94 1.30 2.48 -5.01
Nucleus 0.25 -2.32 1.13 0.47 5.47 -4.56 -6.85 -2.29 2.50 5.04 -2.93 -5.27 -0.32 2.90 5.90
Peroxisomes 4.25 15.73 -0.18 -1.14 -3.04 -2.83 6.21 -2.07 0.75 -4.53 -1.21 17.99 -0.94 -0.23 -4.62
Vacuole 5.78 -4.27 -22.92 -26.19 -20.97 -24.05 -11.67 -28.79 -8.26 -8.68 -20.19 -8.92 -35.95 -14.37 -29.30
Vacuole Periphery 2.77 13.16 3.30 1.21 -4.41 -3.21 14.04 8.15 9.12 -5.67 -1.94 18.73 6.47 7.02 -5.74
Standard name
Human Ortholog
Description Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Gpn2

Gpn2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gpn2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available