| Standard name | |
|---|---|
| Description | Daughter cell-specific protein, may help establish daughter fate; relocalizes from bud neck to cytoplasm upon DNA replication stress |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | 1 | 8 | 2 | 4 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 3 | 1 | 1 | – | – | – | 3 | 5 | 21 | 9 | 11 | 10 |
| Bud | 2 | 0 | 10 | 7 | 8 | 11 | 23 | 11 | 13 | 20 | 15 | 15 | 1 | 1 | 0 | – | – | – | 7 | 9 | 12 | 10 | 9 | 17 |
| Bud Neck | 1 | 0 | 10 | 1 | 8 | 2 | 5 | 3 | 4 | 5 | 1 | 2 | 2 | 1 | 2 | – | – | – | 11 | 14 | 36 | 31 | 13 | 4 |
| Bud Site | 7 | 9 | 18 | 9 | 3 | 4 | 3 | 6 | 3 | 6 | 5 | 1 | 0 | 0 | 1 | – | – | – | – | – | – | – | – | – |
| Cell Periphery | 2 | 0 | 4 | 1 | 2 | 4 | 3 | 0 | 1 | 1 | 0 | 1 | 5 | 1 | 1 | – | – | – | 1 | 0 | 4 | 4 | 7 | 7 |
| Cytoplasm | 92 | 61 | 361 | 199 | 285 | 336 | 340 | 299 | 249 | 330 | 192 | 277 | 357 | 461 | 501 | – | – | – | 67 | 55 | 217 | 203 | 315 | 389 |
| Endoplasmic Reticulum | 0 | 1 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | – | – | – | 0 | 2 | 6 | 0 | 2 | 0 |
| Endosome | 0 | 0 | 5 | 0 | 2 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | – | – | – | 1 | 4 | 8 | 3 | 2 | 4 |
| Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | 0 | 0 | 2 | 0 | 0 | 2 |
| Mitochondria | 29 | 15 | 4 | 8 | 46 | 143 | 248 | 158 | 227 | 263 | 257 | 304 | 0 | 0 | 0 | – | – | – | 12 | 8 | 67 | 15 | 13 | 21 |
| Nucleus | 0 | 1 | 1 | 0 | 2 | 1 | 0 | 0 | 1 | 2 | 0 | 2 | 1 | 1 | 0 | – | – | – | 0 | 0 | 0 | 2 | 5 | 2 |
| Nuclear Periphery | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 1 | 2 |
| Nucleolus | 0 | 1 | 1 | 0 | 1 | 0 | 2 | 1 | 2 | 1 | 0 | 2 | 0 | 0 | 0 | – | – | – | 0 | 0 | 1 | 1 | 1 | 0 |
| Peroxisomes | 3 | 5 | 6 | 6 | 4 | 5 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | – | – | – | 0 | 0 | 1 | 0 | 0 | 1 |
| SpindlePole | 8 | 2 | 31 | 15 | 11 | 12 | 18 | 18 | 16 | 14 | 11 | 13 | 2 | 2 | 4 | – | – | – | 2 | 2 | 16 | 7 | 9 | 12 |
| Vac/Vac Membrane | 0 | 1 | 1 | 1 | 0 | 2 | 2 | 0 | 2 | 1 | 6 | 5 | 0 | 0 | 0 | – | – | – | 1 | 2 | 3 | 6 | 6 | 6 |
| Unique Cell Count | 111 | 82 | 409 | 224 | 329 | 427 | 487 | 398 | 414 | 487 | 365 | 484 | 362 | 464 | 508 | 114 | 111 | 407 | 305 | 413 | 495 | |||
| Labelled Cell Count | 145 | 105 | 454 | 252 | 375 | 522 | 646 | 498 | 519 | 645 | 487 | 625 | 372 | 468 | 513 | 114 | 111 | 407 | 305 | 413 | 495 | |||
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | 3.3 | 4.0 | 3.6 | 3.3 | 3.5 | 3.0 | 2.7 | 2.8 | 2.6 | 2.4 | 2.2 | 2.3 | 4.8 | 5.1 | 4.7 | – | – | – | 4.9 | 5.2 | 5.3 |
| Std Deviation (1e-4) | 0.5 | 0.8 | 1.4 | 1.3 | 1.5 | 1.6 | 1.1 | 1.3 | 1.0 | 0.9 | 0.6 | 0.7 | 0.9 | 1.6 | 1.1 | – | – | – | 1.0 | 1.2 | 1.5 |
| Intensity Change (Log2) | – | – | – | -0.12 | -0.04 | -0.29 | -0.43 | -0.35 | -0.47 | -0.57 | -0.69 | -0.66 | 0.4 | 0.49 | 0.38 | – | – | – | 0.43 | 0.51 | 0.54 |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
External localization resources
