Standard name | |
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Human Ortholog | |
Description | Daughter cell-specific protein, may help establish daughter fate; relocalizes from bud neck to cytoplasm upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 8 | 2 | 4 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 3 | 1 | 1 | – | – | – | 3 | 5 | 21 | 9 | 11 | 10 |
Bud | 2 | 0 | 10 | 7 | 8 | 11 | 23 | 11 | 13 | 20 | 15 | 15 | 1 | 1 | 0 | – | – | – | 7 | 9 | 12 | 10 | 9 | 17 |
Bud Neck | 1 | 0 | 10 | 1 | 8 | 2 | 5 | 3 | 4 | 5 | 1 | 2 | 2 | 1 | 2 | – | – | – | 11 | 14 | 36 | 31 | 13 | 4 |
Bud Site | 7 | 9 | 18 | 9 | 3 | 4 | 3 | 6 | 3 | 6 | 5 | 1 | 0 | 0 | 1 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 2 | 0 | 4 | 1 | 2 | 4 | 3 | 0 | 1 | 1 | 0 | 1 | 5 | 1 | 1 | – | – | – | 1 | 0 | 4 | 4 | 7 | 7 |
Cytoplasm | 92 | 61 | 361 | 199 | 285 | 336 | 340 | 299 | 249 | 330 | 192 | 277 | 357 | 461 | 501 | – | – | – | 67 | 55 | 217 | 203 | 315 | 389 |
Endoplasmic Reticulum | 0 | 1 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | – | – | – | 0 | 2 | 6 | 0 | 2 | 0 |
Endosome | 0 | 0 | 5 | 0 | 2 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | – | – | – | 1 | 4 | 8 | 3 | 2 | 4 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | 0 | 0 | 2 | 0 | 0 | 2 |
Mitochondria | 29 | 15 | 4 | 8 | 46 | 143 | 248 | 158 | 227 | 263 | 257 | 304 | 0 | 0 | 0 | – | – | – | 12 | 8 | 67 | 15 | 13 | 21 |
Nucleus | 0 | 1 | 1 | 0 | 2 | 1 | 0 | 0 | 1 | 2 | 0 | 2 | 1 | 1 | 0 | – | – | – | 0 | 0 | 0 | 2 | 5 | 2 |
Nuclear Periphery | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 1 | 2 |
Nucleolus | 0 | 1 | 1 | 0 | 1 | 0 | 2 | 1 | 2 | 1 | 0 | 2 | 0 | 0 | 0 | – | – | – | 0 | 0 | 1 | 1 | 1 | 0 |
Peroxisomes | 3 | 5 | 6 | 6 | 4 | 5 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | – | – | – | 0 | 0 | 1 | 0 | 0 | 1 |
SpindlePole | 8 | 2 | 31 | 15 | 11 | 12 | 18 | 18 | 16 | 14 | 11 | 13 | 2 | 2 | 4 | – | – | – | 2 | 2 | 16 | 7 | 9 | 12 |
Vac/Vac Membrane | 0 | 1 | 1 | 1 | 0 | 2 | 2 | 0 | 2 | 1 | 6 | 5 | 0 | 0 | 0 | – | – | – | 1 | 2 | 3 | 6 | 6 | 6 |
Unique Cell Count | 111 | 82 | 409 | 224 | 329 | 427 | 487 | 398 | 414 | 487 | 365 | 484 | 362 | 464 | 508 | 114 | 111 | 407 | 305 | 413 | 495 | |||
Labelled Cell Count | 145 | 105 | 454 | 252 | 375 | 522 | 646 | 498 | 519 | 645 | 487 | 625 | 372 | 468 | 513 | 114 | 111 | 407 | 305 | 413 | 495 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.3 | 4.0 | 3.6 | 3.3 | 3.5 | 3.0 | 2.7 | 2.8 | 2.6 | 2.4 | 2.2 | 2.3 | 4.8 | 5.1 | 4.7 | – | – | – | 4.9 | 5.2 | 5.3 |
Std Deviation (1e-4) | 0.5 | 0.8 | 1.4 | 1.3 | 1.5 | 1.6 | 1.1 | 1.3 | 1.0 | 0.9 | 0.6 | 0.7 | 0.9 | 1.6 | 1.1 | – | – | – | 1.0 | 1.2 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.12 | -0.04 | -0.29 | -0.43 | -0.35 | -0.47 | -0.57 | -0.69 | -0.66 | 0.4 | 0.49 | 0.38 | – | – | – | 0.43 | 0.51 | 0.54 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.0847 | 1.0958 | 1.0375 | 0.8169 | 0.4025 | 1.6031 | 0.2429 | -0.7101 | -0.9877 | -0.5905 | -0.1771 | -0.0608 | -0.8982 | -1.2721 | -1.4335 | -1.3442 | -1.4675 | -0.8027 |
Actin | 0.0129 | 0.0003 | 0.0233 | 0.0002 | 0.0002 | 0.0201 | 0.0716 | 0.0077 | 0.0167 | 0.0108 | 0.0005 | 0.0128 | 0.0317 | 0.0102 | 0.0007 | 0.0006 | 0.0165 | 0.0062 |
Bud | 0.0025 | 0.014 | 0.0026 | 0.0001 | 0.0002 | 0.0003 | 0.0022 | 0.0024 | 0.0002 | 0.0004 | 0.0004 | 0.0019 | 0.002 | 0.0069 | 0.0049 | 0.0123 | 0.02 | 0.0018 |
Bud Neck | 0.0683 | 0.0007 | 0.0004 | 0.0001 | 0 | 0.2504 | 0.0576 | 0.0013 | 0.0119 | 0.0001 | 0.0001 | 0.3986 | 0.0457 | 0.0025 | 0.0011 | 0.0005 | 0.037 | 0.3404 |
Bud Periphery | 0.0107 | 0.0203 | 0.0106 | 0.0001 | 0.0004 | 0.0008 | 0.005 | 0.0053 | 0.0002 | 0.0003 | 0.0005 | 0.0009 | 0.005 | 0.0047 | 0.0041 | 0.0036 | 0.0097 | 0.001 |
Bud Site | 0.0327 | 0.0384 | 0.0224 | 0 | 0 | 0.0012 | 0.0991 | 0.0747 | 0.0229 | 0.0003 | 0.0012 | 0.0033 | 0.0316 | 0.0455 | 0.0109 | 0.0214 | 0.0029 | 0.0032 |
Cell Periphery | 0.0017 | 0.0009 | 0.0004 | 0 | 0.0001 | 0.0004 | 0.003 | 0.0008 | 0.0002 | 0 | 0.0002 | 0.0003 | 0.0013 | 0.001 | 0.0003 | 0.0003 | 0.0001 | 0.0003 |
Cytoplasm | 0.0578 | 0.0353 | 0.0125 | 0.0143 | 0.0316 | 0.0539 | 0.0732 | 0.0834 | 0.0616 | 0.056 | 0.1602 | 0.0803 | 0.0559 | 0.0672 | 0.0348 | 0.018 | 0.041 | 0.0679 |
Cytoplasmic Foci | 0.0084 | 0.0017 | 0.0036 | 0.0019 | 0.0048 | 0.0066 | 0.036 | 0.0177 | 0.005 | 0.011 | 0.0047 | 0.0272 | 0.0424 | 0.0049 | 0.0127 | 0.0272 | 0.0146 | 0.0134 |
Eisosomes | 0.0009 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.0015 | 0.0004 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0011 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0003 |
Endoplasmic Reticulum | 0.0034 | 0.0003 | 0.0009 | 0.0001 | 0.0007 | 0.001 | 0.0063 | 0.0008 | 0.0005 | 0.0024 | 0.0034 | 0.0011 | 0.0038 | 0.0004 | 0.0007 | 0.0004 | 0.0006 | 0.0003 |
Endosome | 0.0157 | 0.0003 | 0.006 | 0.0001 | 0.0011 | 0.0029 | 0.036 | 0.0034 | 0.003 | 0.0226 | 0.0063 | 0.014 | 0.0103 | 0.0008 | 0.0006 | 0.0024 | 0.0046 | 0.0008 |
Golgi | 0.0016 | 0.0001 | 0.0038 | 0 | 0.0001 | 0.0019 | 0.0205 | 0.002 | 0.0031 | 0.0111 | 0.0005 | 0.0076 | 0.0075 | 0.0002 | 0.0001 | 0.0007 | 0.0027 | 0.0009 |
Lipid Particles | 0.0138 | 0.0001 | 0.0004 | 0.0001 | 0.0003 | 0.0012 | 0.0299 | 0.0039 | 0.0009 | 0.009 | 0.0003 | 0.0073 | 0.0086 | 0.0001 | 0.0004 | 0.0032 | 0.005 | 0.0034 |
Mitochondria | 0.0051 | 0.0007 | 0.0034 | 0.0001 | 0.0003 | 0.0004 | 0.0162 | 0.0018 | 0.0008 | 0.0018 | 0.0009 | 0.0083 | 0.0017 | 0.0003 | 0.0019 | 0.0006 | 0.0026 | 0.0009 |
None | 0.7395 | 0.8839 | 0.9062 | 0.9816 | 0.9579 | 0.6499 | 0.5028 | 0.7811 | 0.8705 | 0.8675 | 0.8132 | 0.4173 | 0.727 | 0.8495 | 0.907 | 0.8985 | 0.8026 | 0.5455 |
Nuclear Periphery | 0.0088 | 0.0002 | 0.0002 | 0.0001 | 0.0004 | 0.0038 | 0.0168 | 0.0006 | 0.0001 | 0.0017 | 0.0015 | 0.0015 | 0.0035 | 0.0002 | 0.0009 | 0.0003 | 0.0021 | 0.0004 |
Nucleolus | 0.0029 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0008 | 0.0009 | 0.0006 | 0.0001 | 0.0001 | 0.0002 | 0.0005 | 0.0011 | 0.0005 | 0.0017 | 0.0006 | 0.0007 | 0.001 |
Nucleus | 0.0027 | 0.0007 | 0.0002 | 0.0001 | 0.0003 | 0.0005 | 0.0033 | 0.0008 | 0.0002 | 0.0004 | 0.0028 | 0.0021 | 0.0018 | 0.0015 | 0.0036 | 0.0028 | 0.0015 | 0.0016 |
Peroxisomes | 0.0018 | 0.0002 | 0.0014 | 0.0001 | 0.0001 | 0.0006 | 0.0073 | 0.0076 | 0.0007 | 0.002 | 0.0002 | 0.0107 | 0.0105 | 0.001 | 0.0004 | 0.0045 | 0.031 | 0.0064 |
Punctate Nuclear | 0.0059 | 0.0004 | 0.0006 | 0.0008 | 0.0002 | 0.0027 | 0.0048 | 0.0028 | 0.0004 | 0.0015 | 0.0005 | 0.0033 | 0.0052 | 0.0015 | 0.0124 | 0.0016 | 0.0038 | 0.0037 |
Vacuole | 0.002 | 0.0007 | 0.0004 | 0.0001 | 0.001 | 0.0002 | 0.0037 | 0.0007 | 0.0005 | 0.0005 | 0.0021 | 0.0006 | 0.0017 | 0.0009 | 0.0007 | 0.0005 | 0.0006 | 0.0004 |
Vacuole Periphery | 0.001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0025 | 0.0001 | 0.0001 | 0.0003 | 0.0004 | 0.0004 | 0.0006 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 5.9736 | 0.7629 | 22.0769 | 82.7626 | 35.4057 | 2.5587 | 2.5068 | 3.9044 | 42.3922 | 57.1199 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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