Standard name
Human Ortholog
Description Protein kinase; implicated in activation of the plasma membrane H(+)-ATPase Pma1p in response to glucose metabolism; plays a role in ion homeostasis; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.08 0.14 0.09 0 0.24 0.11 0.2 0.11 0.15 0.11 0.09 0.15 0.45 0.56 0.39 0.17 0.26 0.17 0 0 0 0 0 0
Cytoplasm 0.97 0.99 0.98 0.96 0.97 0.94 0.9 0.86 0.82 0.81 0.8 0.84 0.97 0.98 0.98 0.94 0.89 0.97 0.94 0.96 0.93 0.92 0.89 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0.05 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0.07 0.17 0.18 0.19 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 7 1 0 1 5 0 1 0 0 0 0 0 0 1 0 2 2
Bud 0 2 2 1 0 0 2 3 6 5 6 6 4 2 2 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 9 22 12 3 39 15 46 30 37 20 21 37 120 134 108 25 37 15 0 0 0 4 2 4
Cytoplasm 114 150 135 72 160 128 207 243 199 154 182 209 257 235 269 135 125 85 111 148 135 166 237 247
Endoplasmic Reticulum 1 3 1 0 1 3 1 12 4 1 3 6 3 0 2 9 8 2 1 0 2 3 12 9
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 0 1 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2
Mitochondria 2 1 0 1 1 4 8 20 41 35 43 30 1 2 0 1 0 1 0 0 1 0 1 1
Nucleus 2 0 0 3 0 0 1 2 2 0 0 3 0 0 1 2 7 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 1 1 0 0 6 2 4 5 0 0 0 1 4 5 0 1 1 0 2 2 3
Unique Cell Count 117 152 138 75 165 136 230 283 242 190 227 250 264 241 275 144 140 88 119 154 146 182 266 281
Labelled Cell Count 128 179 150 81 202 150 266 323 292 220 261 296 385 376 384 176 182 103 119 154 146 182 266 281


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.1 10.7 8.8 7.6 9.1 7.1 7.5 7.5 6.5 6.0 6.0 6.3 10.1 11.5 11.7 12.4 12.2 12.3 9.6 10.2 11.6
Std Deviation (1e-4) 1.5 2.0 1.3 1.0 1.2 1.1 1.1 1.1 1.1 1.1 1.1 0.9 1.5 1.8 1.9 2.5 2.1 2.7 1.5 1.6 1.9
Intensity Change (Log2) – – – -0.21 0.04 -0.32 -0.23 -0.22 -0.43 -0.55 -0.54 -0.48 0.2 0.38 0.41 0.49 0.48 0.48 0.12 0.21 0.39


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.3 3.5 0.6 2.9 0.6 1.8 0.6 0.2 1.7 7.5 9.0 6.5 2.2 3.9 1.9
Cytoplasm -0.8 -0.5 -1.6 -2.8 -3.8 -4.5 -4.6 -4.8 -4.2 -0.3 -0.2 0 -1.7 -2.9 -0.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 3.2 5.1 5.3 5.4 4.2 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.7828 6.5487 5.9059 5.3042 4.7897 5.6402 5.7537 8.0031 6.984 6.6554 6.7469 7.0594 4.8381 6.3594 6.0156 5.4882 5.5896 5.4321
Actin 0.0107 0.0014 0.0117 0.0371 0.0229 0.0053 0.0181 0.0009 0.0079 0.0007 0.0247 0.0017 0.0277 0.0065 0.0176 0.0184 0.0002 0.0209
Bud 0.0002 0.0003 0.0004 0.0002 0.0003 0.0002 0.0008 0.0002 0.0009 0.0002 0.0011 0.0002 0.0003 0.0009 0.0019 0.0003 0 0.0001
Bud Neck 0.0023 0.0002 0.0006 0.0024 0.0013 0.0019 0.0023 0.0003 0.0035 0.0006 0.0042 0.0015 0.0003 0.0004 0.0012 0.0021 0.0004 0.001
Bud Periphery 0.0003 0.0001 0.0002 0.0001 0.0002 0.0003 0.0009 0.0001 0.001 0.0001 0.0008 0.0003 0.0006 0.0003 0.0023 0.0002 0 0.0001
Bud Site 0.0012 0.0076 0.001 0.0005 0.0013 0.0003 0.0034 0.0033 0.0075 0.0002 0.0055 0.0003 0.0012 0.0132 0.0072 0.0139 0 0.0001
Cell Periphery 0.0015 0.0006 0.0006 0.0002 0.0003 0.0003 0.0024 0.0005 0.0008 0.0003 0.0004 0.0003 0.0021 0.0008 0.0045 0.0002 0.0001 0.0003
Cytoplasm 0.8824 0.9758 0.9506 0.9248 0.8697 0.9168 0.7598 0.9885 0.917 0.9573 0.7945 0.9449 0.8631 0.9635 0.9274 0.8829 0.9954 0.9614
Cytoplasmic Foci 0.0196 0.0044 0.0082 0.0112 0.0396 0.005 0.0445 0.0017 0.0104 0.0041 0.0439 0.0061 0.0228 0.0024 0.0058 0.0081 0.0008 0.0035
Eisosomes 0.0001 0 0.0002 0.0003 0.0001 0.0003 0.0003 0 0.0001 0 0.0001 0 0.0002 0.0001 0.0002 0.0002 0 0
Endoplasmic Reticulum 0.0171 0.002 0.0048 0.0022 0.005 0.0078 0.0088 0.0014 0.004 0.0038 0.0019 0.0038 0.0109 0.004 0.0051 0.0014 0.0016 0.0068
Endosome 0.0209 0.0015 0.0053 0.0081 0.0246 0.0171 0.0518 0.0007 0.0169 0.0058 0.0356 0.0148 0.0218 0.0008 0.0043 0.001 0.0003 0.0021
Golgi 0.007 0.0004 0.003 0.0015 0.0053 0.002 0.0291 0.0002 0.0079 0.0114 0.0188 0.0083 0.0126 0.0006 0.0021 0.0017 0.0001 0.001
Lipid Particles 0.0043 0.0002 0.0021 0.0012 0.0063 0.0018 0.0231 0.0001 0.0083 0.0008 0.0375 0.0018 0.0078 0.0002 0.0016 0.007 0 0.0001
Mitochondria 0.0028 0.0001 0.0003 0.0002 0.0008 0.0019 0.0183 0 0.0038 0.0086 0.0069 0.0126 0.0019 0.0001 0.0003 0.0258 0 0.0002
None 0.011 0.0035 0.0026 0.0015 0.0028 0.0077 0.0036 0.0011 0.0014 0.0012 0.0012 0.0007 0.014 0.0053 0.0065 0.0015 0.0005 0.001
Nuclear Periphery 0.004 0.0002 0.0022 0.0004 0.0012 0.0134 0.0119 0.0001 0.0008 0.0003 0.0006 0.0002 0.0013 0.0002 0.0009 0.0001 0.0001 0.0002
Nucleolus 0.0003 0 0.0001 0 0.0003 0.0017 0.0004 0 0.0009 0 0.0013 0.0001 0.0001 0 0.0004 0.0003 0 0
Nucleus 0.0062 0.0004 0.0009 0.0006 0.001 0.0058 0.0011 0.0003 0.0006 0.0005 0.0014 0.0003 0.0004 0.0003 0.001 0.0003 0.0002 0.0002
Peroxisomes 0.0012 0 0.0013 0.0018 0.0067 0.0003 0.0112 0 0.0033 0.0025 0.0121 0.0007 0.0068 0 0.0044 0.0341 0 0.0001
Punctate Nuclear 0.0037 0.0001 0.0011 0.0006 0.0089 0.0062 0.0011 0 0.0005 0.0001 0.004 0.0001 0.003 0.0001 0.0006 0.0001 0 0.0001
Vacuole 0.0029 0.001 0.0026 0.0045 0.0012 0.0027 0.0044 0.0005 0.0022 0.0013 0.003 0.001 0.001 0.0004 0.0044 0.0004 0.0002 0.0005
Vacuole Periphery 0.0004 0.0001 0.0002 0.0003 0.0002 0.0013 0.0025 0 0.0005 0.0002 0.0006 0.0004 0.0004 0 0.0003 0.0001 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 26.1333 17.7375 21.8296 39.3916 18.8238 29.5046 29.3073 48.1226 34.7514 40.7293
Translational Efficiency 0.6649 0.8417 0.8353 0.5385 0.7793 0.8501 0.9344 0.5438 0.8101 0.5424

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1591 1612 1089 1184 1537 830 1671 2961 3128 2442 2760 4145

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 961.94 1195.25 1478.45 1600.55 971.00 1181.36 1252.64 1547.09 966.39 1190.53 1341.74 1562.36
Standard Deviation 129.46 182.95 193.13 273.76 212.49 174.91 207.59 251.55 175.30 180.37 230.19 259.22
Intensity Change Log 2 — 0.313294 0.620067 0.734549 — 0.282905 0.367429 0.672014 — 0.298108 0.498680 0.703474

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000269 0.000631 0.000797 0.001610 0.000619 0.002394 0.000254 0.000821 0.000441 0.001230 0.000468 0.001046
Bud Neck 0.001333 0.001561 0.001397 0.002094 0.003601 0.003011 0.001753 0.001529 0.002448 0.002054 0.001613 0.001690
Bud Site 0.023814 0.023659 0.013052 0.027144 0.030514 0.051310 0.010162 0.015752 0.027106 0.033057 0.011302 0.019006
Cell Periphery 0.000317 0.000510 0.000302 0.003289 0.000921 0.002485 0.000150 0.000482 0.000614 0.001181 0.000210 0.001284
Cytoplasm 0.833296* 0.860509* 0.861954* 0.872575* 0.773342* 0.775939* 0.623532* 0.935514* 0.803836* 0.831765* 0.717605* 0.917536*
Cytoplasmic Foci 0.092103 0.068384 0.041481 0.017583 0.104466 0.112044 0.057652 0.007402 0.098178 0.083224 0.051271 0.010310
Eisosomes 0.000024 0.000029 0.000008 0.000018 0.000108 0.000102 0.000027 0.000008 0.000065 0.000054 0.000019 0.000011
Endoplasmic Reticulum 0.001363 0.000665 0.001243 0.000092 0.003342 0.001248 0.002261 0.000028 0.002335 0.000863 0.001859 0.000046
Endosome 0.004345 0.002365 0.002463 0.001219 0.007650 0.004844 0.008316 0.000245 0.005969 0.003207 0.006007 0.000523
Golgi 0.006225 0.006267 0.001193 0.001451 0.008662 0.007358 0.001076 0.000344 0.007423 0.006638 0.001122 0.000660
Lipid Particles 0.000940 0.001625 0.002759 0.011202 0.003041 0.001857 0.001810 0.001398 0.001972 0.001704 0.002185 0.004198
Mitochondria 0.000361 0.000266 0.000022 0.000068 0.000490 0.000255 0.000154 0.000072 0.000425 0.000263 0.000102 0.000071
Mitotic Spindle 0.000160 0.001412 0.002022 0.001939 0.000585 0.002462 0.000715 0.000233 0.000369 0.001769 0.001231 0.000721
None 0.001690 0.002425 0.001308 0.000548 0.009486 0.000908 0.002650 0.001144 0.005521 0.001910 0.002121 0.000974
Nuclear Periphery 0.000011 0.000012 0.000082 0.000037 0.000159 0.000038 0.000182 0.000012 0.000084 0.000021 0.000142 0.000019
Nuclear Periphery Foci 0.000846 0.001273 0.005744 0.004725 0.002303 0.001407 0.003033 0.000655 0.001562 0.001319 0.004102 0.001817
Nucleolus 0.000128 0.000129 0.000058 0.000065 0.000773 0.000277 0.000175 0.000111 0.000445 0.000179 0.000129 0.000098
Nucleus 0.005477 0.006448 0.018791 0.007208 0.015063 0.004315 0.026685 0.007550 0.010187 0.005723 0.023570 0.007452
Peroxisomes 0.000606 0.000764 0.000421 0.000483 0.000850 0.001331 0.000528 0.000589 0.000726 0.000957 0.000486 0.000559
Vacuole 0.024756 0.020274 0.044635 0.045800 0.031394 0.024988 0.258371* 0.025921 0.028018 0.021876 0.174038 0.031599
Vacuole Periphery 0.001937 0.000790 0.000268 0.000850 0.002631 0.001427 0.000515 0.000192 0.002278 0.001006 0.000418 0.000380

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.29 -5.82 -5.59 -4.07 -3.17 -4.26 5.94 -0.76 3.25 -2.09 -5.25 -1.26 -3.20 0.61 -2.85
Bud Neck -1.00 -4.24 -7.08 -3.54 -3.05 1.52 5.85 6.25 4.58 1.47 1.78 3.54 3.45 1.30 0.52
Bud Site 0.08 5.61 -2.52 -2.57 -8.73 -7.83 12.13 8.99 15.90 -5.64 -3.87 13.18 6.89 10.71 -8.81
Cell Periphery -4.72 0.18 -4.37 -4.04 -4.38 -1.61 13.34 7.02 2.09 -9.39 -1.70 12.76 -3.10 0.08 -5.51
Cytoplasm -4.56 1.36 -2.65 1.55 -3.68 -0.39 17.26 -28.47 -22.71 -42.91* -5.68 16.51 -26.21 -18.98 -37.70
Cytoplasmic Foci 6.08 12.30 22.38 18.05 12.57 -1.52 12.77 31.24 25.80 27.76* 4.89 17.15 39.45 32.67 30.91*
Eisosomes -1.98 7.56 1.25 2.53 -2.64 0.45 7.38 9.03 14.13 13.17 1.80 8.19 9.56 14.87 5.96
Endoplasmic Reticulum 1.98 0.55 4.08 3.53 1.88 2.90 2.00 5.28 3.37 5.12 3.84 1.71 6.60 4.99 5.08
Endosome 4.74 3.84 7.21 3.40 4.92 3.56 0.45 13.47 7.85 12.01 6.77 0.90 16.40 10.14 13.42
Golgi -0.03 16.84 12.14 6.45 -0.39 1.76 17.70 26.93 10.27 1.82 1.39 22.43 29.63 11.20 1.89
Lipid Particles -2.52 -10.17 -9.63 -8.88 -7.28 3.06 3.30 3.45 0.75 0.97 1.07 -2.64 -6.29 -6.97 -5.27
Mitochondria 0.86 3.59 3.16 3.24 -1.38 1.88 3.50 4.98 1.97 0.94 2.01 4.63 5.62 3.77 0.56
Mitotic Spindle -2.67 -6.36 -2.37 -0.51 1.19 -1.47 -0.39 1.41 1.77 2.08 -2.60 -4.86 -1.37 1.89 2.85
None -2.10 2.17 4.95 6.33 3.74 5.30 4.20 5.22 -0.76 1.40 4.41 4.16 5.71 4.31 1.79
Nuclear Periphery -0.49 -14.21 -5.14 -4.80 8.54 2.78 -0.15 4.01 0.93 3.23 3.21 -1.99 3.45 -0.16 4.45
Nuclear Periphery Foci -1.94 -14.53 -11.59 -10.18 3.24 1.97 -1.35 3.21 5.28 10.37 1.06 -9.15 -1.52 -4.47 10.94
Nucleolus 0.02 3.30 3.27 5.66 0.28 2.15 5.81 6.35 0.75 3.84 3.03 6.04 6.63 1.09 3.17
Nucleus -2.86 -17.84 -6.68 -4.76 13.18 6.47 -5.23 3.90 -6.65 11.93 5.48 -11.85 1.57 -8.71 16.30
Peroxisomes -1.99 1.31 1.71 4.23 -0.52 -4.77 4.26 3.36 6.34 -0.38 -3.92 3.12 2.81 5.75 -0.72
Vacuole 2.58 -11.43 -12.07 -14.69 1.47 2.61 -36.27 1.31 -2.05 37.46* 4.46 -34.99 -7.36 -12.82 32.86
Vacuole Periphery 4.70 7.71 3.10 0.33 -1.20 1.88 5.15 5.80 2.57 4.80 4.30 8.07 7.66 3.31 0.46
Standard name
Human Ortholog
Description Protein kinase; implicated in activation of the plasma membrane H(+)-ATPase Pma1p in response to glucose metabolism; plays a role in ion homeostasis; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Hrk1

Hrk1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hrk1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available