Standard name | |
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Description | Protein kinase; implicated in activation of the plasma membrane H(+)-ATPase Pma1p in response to glucose metabolism; plays a role in ion homeostasis; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 7 | 1 | 0 | 1 | 5 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 2 |
Bud | 0 | 2 | 2 | 1 | 0 | 0 | 2 | 3 | 6 | 5 | 6 | 6 | 4 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 9 | 22 | 12 | 3 | 39 | 15 | 46 | 30 | 37 | 20 | 21 | 37 | 120 | 134 | 108 | 25 | 37 | 15 | 0 | 0 | 0 | 4 | 2 | 4 |
Cytoplasm | 114 | 150 | 135 | 72 | 160 | 128 | 207 | 243 | 199 | 154 | 182 | 209 | 257 | 235 | 269 | 135 | 125 | 85 | 111 | 148 | 135 | 166 | 237 | 247 |
Endoplasmic Reticulum | 1 | 3 | 1 | 0 | 1 | 3 | 1 | 12 | 4 | 1 | 3 | 6 | 3 | 0 | 2 | 9 | 8 | 2 | 1 | 0 | 2 | 3 | 12 | 9 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 |
Mitochondria | 2 | 1 | 0 | 1 | 1 | 4 | 8 | 20 | 41 | 35 | 43 | 30 | 1 | 2 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 1 |
Nucleus | 2 | 0 | 0 | 3 | 0 | 0 | 1 | 2 | 2 | 0 | 0 | 3 | 0 | 0 | 1 | 2 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 6 | 2 | 4 | 5 | 0 | 0 | 0 | 1 | 4 | 5 | 0 | 1 | 1 | 0 | 2 | 2 | 3 |
Unique Cell Count | 117 | 152 | 138 | 75 | 165 | 136 | 230 | 283 | 242 | 190 | 227 | 250 | 264 | 241 | 275 | 144 | 140 | 88 | 119 | 154 | 146 | 182 | 266 | 281 |
Labelled Cell Count | 128 | 179 | 150 | 81 | 202 | 150 | 266 | 323 | 292 | 220 | 261 | 296 | 385 | 376 | 384 | 176 | 182 | 103 | 119 | 154 | 146 | 182 | 266 | 281 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.1 | 10.7 | 8.8 | 7.6 | 9.1 | 7.1 | 7.5 | 7.5 | 6.5 | 6.0 | 6.0 | 6.3 | 10.1 | 11.5 | 11.7 | 12.4 | 12.2 | 12.3 | 9.6 | 10.2 | 11.6 |
Std Deviation (1e-4) | 1.5 | 2.0 | 1.3 | 1.0 | 1.2 | 1.1 | 1.1 | 1.1 | 1.1 | 1.1 | 1.1 | 0.9 | 1.5 | 1.8 | 1.9 | 2.5 | 2.1 | 2.7 | 1.5 | 1.6 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.21 | 0.04 | -0.32 | -0.23 | -0.22 | -0.43 | -0.55 | -0.54 | -0.48 | 0.2 | 0.38 | 0.41 | 0.49 | 0.48 | 0.48 | 0.12 | 0.21 | 0.39 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.3 | 3.5 | 0.6 | 2.9 | 0.6 | 1.8 | 0.6 | 0.2 | 1.7 | 7.5 | 9.0 | 6.5 | 2.2 | 3.9 | 1.9 |
Cytoplasm | -0.8 | -0.5 | -1.6 | -2.8 | -3.8 | -4.5 | -4.6 | -4.8 | -4.2 | -0.3 | -0.2 | 0 | -1.7 | -2.9 | -0.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 3.2 | 5.1 | 5.3 | 5.4 | 4.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |