Standard name
Description Protein kinase; implicated in activation of the plasma membrane H(+)-ATPase Pma1p in response to glucose metabolism; plays a role in ion homeostasis; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.08 0.14 0.09 0 0.24 0.11 0.2 0.11 0.15 0.11 0.09 0.15 0.45 0.56 0.39 0.17 0.26 0.17 0 0 0 0 0 0
Cytoplasm 0.97 0.99 0.98 0.96 0.97 0.94 0.9 0.86 0.82 0.81 0.8 0.84 0.97 0.98 0.98 0.94 0.89 0.97 0.94 0.96 0.93 0.92 0.89 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0.05 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0.07 0.17 0.18 0.19 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 7 1 0 1 5 0 1 0 0 0 0 0 0 1 0 2 2
Bud 0 2 2 1 0 0 2 3 6 5 6 6 4 2 2 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 9 22 12 3 39 15 46 30 37 20 21 37 120 134 108 25 37 15 0 0 0 4 2 4
Cytoplasm 114 150 135 72 160 128 207 243 199 154 182 209 257 235 269 135 125 85 111 148 135 166 237 247
Endoplasmic Reticulum 1 3 1 0 1 3 1 12 4 1 3 6 3 0 2 9 8 2 1 0 2 3 12 9
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 0 1 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2
Mitochondria 2 1 0 1 1 4 8 20 41 35 43 30 1 2 0 1 0 1 0 0 1 0 1 1
Nucleus 2 0 0 3 0 0 1 2 2 0 0 3 0 0 1 2 7 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 1 1 0 0 6 2 4 5 0 0 0 1 4 5 0 1 1 0 2 2 3
Unique Cell Count 117 152 138 75 165 136 230 283 242 190 227 250 264 241 275 144 140 88 119 154 146 182 266 281
Labelled Cell Count 128 179 150 81 202 150 266 323 292 220 261 296 385 376 384 176 182 103 119 154 146 182 266 281


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.1 10.7 8.8 7.6 9.1 7.1 7.5 7.5 6.5 6.0 6.0 6.3 10.1 11.5 11.7 12.4 12.2 12.3 9.6 10.2 11.6
Std Deviation (1e-4) 1.5 2.0 1.3 1.0 1.2 1.1 1.1 1.1 1.1 1.1 1.1 0.9 1.5 1.8 1.9 2.5 2.1 2.7 1.5 1.6 1.9
Intensity Change (Log2) – – – -0.21 0.04 -0.32 -0.23 -0.22 -0.43 -0.55 -0.54 -0.48 0.2 0.38 0.41 0.49 0.48 0.48 0.12 0.21 0.39


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.3 3.5 0.6 2.9 0.6 1.8 0.6 0.2 1.7 7.5 9.0 6.5 2.2 3.9 1.9
Cytoplasm -0.8 -0.5 -1.6 -2.8 -3.8 -4.5 -4.6 -4.8 -4.2 -0.3 -0.2 0 -1.7 -2.9 -0.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 3.2 5.1 5.3 5.4 4.2 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0