ORF
Description Phosphatase with a broad substrate specificity; has some similarity to GPM1/YKL152C, a phosphoglycerate mutase; YOR283W is not an essential gene

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.95 0.99 0.98 0.95 0.89 0.86 0.75 0.69 0.66 0.65 0.53 0.99 1.0 0.99 0.89 0.96 0.97 0.92 0.94 0.97 0.95 0.94 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0.06 0 0.05 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0.05 0.06 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.05 0.1 0.11 0.24 0.2 0.28 0.21 0.32 0 0 0 0.09 0.05 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 2 1 1 9 5 0 0 0 0 0 0 2 0 0 0 0 0
Bud 0 0 0 0 1 1 0 0 0 3 0 0 0 0 0 0 0 1 0 0 0 2 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1 0 1 1 2 1 3 4 9 4 3 2 1 1 1 1 1 0 0 0 0 0 0
Cytoplasm 169 235 184 259 293 194 337 212 245 253 215 193 315 377 49 74 66 176 219 183 161 267 213
Endoplasmic Reticulum 1 1 1 6 10 3 16 10 13 17 15 2 2 2 2 0 2 0 0 0 1 3 5
Endosome 0 0 0 0 0 0 0 0 0 2 2 0 0 0 0 0 0 0 1 0 0 0 0
Golgi 1 1 0 0 0 1 0 1 5 2 4 0 0 1 0 0 0 0 0 0 0 1 0
Mitochondria 1 0 0 0 0 3 2 17 7 19 33 0 0 0 0 1 0 0 1 0 1 0 0
Nucleus 4 1 3 5 6 0 22 17 13 19 21 0 0 1 2 0 0 3 6 2 1 2 6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 6 0 2 14 32 25 106 60 104 83 130 0 0 1 5 4 2 2 2 1 1 1 5
Unique Cell Count 178 237 188 272 328 226 450 306 372 387 403 194 316 380 55 77 68 192 235 189 170 283 238
Labelled Cell Count 183 238 191 285 344 229 488 322 398 411 429 197 318 383 60 80 71 192 235 189 170 283 238


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 22.5 23.6 20.7 21.3 21.3 13.5 15.2 12.1 11.8 11.5 11.1 24.5 24.7 23.9 22.3 23.9 23.9 24.2 23.5 23.9
Std Deviation (1e-4) 6.6 4.5 3.3 3.5 4.1 3.3 2.7 2.1 2.0 2.0 1.9 4.4 4.3 4.1 5.8 6.3 6.0 6.3 5.4 5.6
Intensity Change (Log2) 0.04 0.04 -0.62 -0.45 -0.77 -0.81 -0.85 -0.9 0.24 0.26 0.2 0.11 0.21 0.2 0.22 0.18 0.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.5 -3.5 -4.3 -6.8 -7.7 -8.5 -8.6 -10.8 1.4 2.0 1.4 -2.9 -0.8 -0.4
Endoplasmic Reticulum 0 0 0 2.2 0 0 2.5 2.2 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 3.3 0 3.1 4.0 0 0 0 0 0 0
Nucleus 0 0 0 2.0 2.2 1.3 1.9 2.1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.3 3.8 4.1 6.9 6.0 7.7 6.5 8.5 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yor283w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available