Standard name
Human Ortholog
Description Thiosulfate sulfurtransferase; contains a rhodanese-like domain; localized to the mitochondrial outer membrane; protein abundance increases in response to DNA replication stress; similar to the human TSTD gene

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.07 0.07 0.13 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0.06 0.06 0.05
Bud 0.07 0.11 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.39 0.57 0.45 0.38 0.35 0.23 0.14 0.17 0.18 0.15 0.21 0.63 0.65 0.61 0.47 0.13 0.58 0.18 0.27 0.43 0.21 0.23 0.24
Cytoplasm 0.51 0.52 0.44 0.55 0.43 0.64 0.62 0.73 0.63 0.63 0.65 0.5 0.53 0.54 0.84 0.92 0.74 0.33 0.4 0.13 0.33 0.24 0.3
Endoplasmic Reticulum 0.26 0.18 0.18 0.28 0.43 0.27 0.32 0.19 0.26 0.21 0.24 0.17 0.2 0.16 0 0 0.05 0.37 0.19 0.29 0.34 0.42 0.33
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0.08 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 13 3 25 4 2 4 9 4 17 6 16 4 2 1 0 0 0 11 3 10 11 23 19
Bud 13 5 18 6 8 6 10 3 10 12 8 11 6 4 0 2 0 5 0 4 2 1 9
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 3 4 5 4 8
Bud Site 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 73 25 84 66 88 61 60 44 70 47 80 219 303 320 46 5 25 43 23 90 40 86 100
Cytoplasm 95 23 82 96 108 167 268 192 249 194 254 174 249 282 82 35 32 78 35 26 64 88 129
Endoplasmic Reticulum 49 8 33 49 108 71 136 50 104 66 92 60 91 82 4 0 2 86 16 61 65 157 142
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1
Golgi 0 0 0 0 0 0 2 0 1 0 1 0 0 11 0 0 1 0 0 0 0 0 1
Mitochondria 0 0 3 0 0 1 2 3 7 26 18 0 0 0 1 0 0 0 0 6 2 2 2
Nucleus 0 0 0 0 1 0 0 0 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 2 1 0 0 2 1 1 0 0 0 0 0 0 0 1 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 3 0 0 0 2 1 4 1 1 0 0 24 0 2 1 1 0 1 1 2 6
Unique Cell Count 186 44 186 175 254 262 431 264 393 308 390 349 466 524 98 38 43 236 87 210 196 375 428
Labelled Cell Count 243 64 251 222 315 310 491 299 463 354 471 469 651 724 133 44 62 236 87 210 196 375 428


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 25.7 34.3 18.9 25.5 31.1 28.0 27.7 26.2 26.1 24.1 23.5 36.3 40.9 45.1 58.1 45.8 56.9 27.3 29.8 31.6
Std Deviation (1e-4) 5.3 4.9 5.5 6.0 7.6 6.7 6.7 7.7 6.9 6.8 6.5 9.4 9.6 10.1 22.2 21.4 20.3 7.7 8.0 9.8
Intensity Change (Log2) 0.43 0.72 0.57 0.55 0.47 0.47 0.35 0.31 0.94 1.12 1.26 1.62 1.28 1.59 0.53 0.66 0.74


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -3.9 -5.5 -5.1 -5.7 -5.1 -3.9 -5.1 -4.1 -6.0 -7.5 -8.3 -3.8 0 0
Bud -2.4 -2.9 -3.4 -4.0 -4.2 -3.7 -2.6 -4.1 -3.2 -5.1 -6.0 -3.2 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.4 -2.2 -4.9 -8.4 -6.6 -7.0 -7.3 -6.1 3.9 4.7 3.8 0.3 -3.7 1.5
Cytoplasm 2.0 -0.3 4.1 4.2 6.1 4.4 4.1 4.8 1.3 2.2 2.3 6.4 5.4 3.6
Endoplasmic Reticulum 2.3 5.5 2.3 3.5 0.3 2.3 1.0 1.6 -0.2 0.5 -0.7 -3.3 -2.8 -2.2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 3.1 1.8 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 1.8 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 23.2623 28.5399 24.9606 25.788 21.4244 26.5521 22.3636 26.5081 23.7701 24.3478 22.0781 25.2654 23.256 27.1267 25.7104 24.2697 23.6784 26.9352
Actin 0.0585 0.0382 0.0573 0.0518 0.0573 0.0312 0.0555 0.0184 0.0569 0.0216 0.0803 0.0282 0.0517 0.0164 0.0601 0.085 0.0105 0.06
Bud 0.0045 0.0011 0.0195 0.0137 0.0038 0.002 0.0048 0.0015 0.0126 0.0044 0.0025 0.0014 0.0043 0.0013 0.0284 0.0071 0.0058 0.0019
Bud Neck 0.0075 0.0123 0.0149 0.0391 0.0564 0.0888 0.0061 0.0071 0.0128 0.0128 0.0399 0.1038 0.0105 0.0088 0.0179 0.0228 0.0108 0.2309
Bud Periphery 0.0113 0.0019 0.0529 0.0685 0.0291 0.0102 0.0225 0.0034 0.0394 0.0243 0.0195 0.01 0.0118 0.003 0.1065 0.0498 0.0303 0.0129
Bud Site 0.0197 0.0855 0.0276 0.0061 0.0032 0.0016 0.021 0.1066 0.0409 0.0029 0.0013 0.0014 0.0147 0.0761 0.0377 0.0043 0.0027 0.0015
Cell Periphery 0.1328 0.2208 0.1986 0.2268 0.1854 0.0959 0.1797 0.2701 0.2378 0.2768 0.154 0.1667 0.1897 0.2254 0.2352 0.28 0.2487 0.1616
Cytoplasm 0.4775 0.4688 0.4164 0.2814 0.3001 0.404 0.3154 0.409 0.3284 0.3798 0.3347 0.3311 0.3865 0.5171 0.389 0.2805 0.4123 0.2803
Cytoplasmic Foci 0.0152 0.0045 0.0071 0.0034 0.0171 0.0064 0.0137 0.0016 0.0072 0.0041 0.0075 0.0038 0.0088 0.0021 0.0026 0.0036 0.0016 0.0037
Eisosomes 0.0014 0.0012 0.0008 0.0015 0.0011 0.0017 0.0034 0.002 0.002 0.0007 0.0051 0.0021 0.0018 0.0011 0.0009 0.0006 0.0009 0.0035
Endoplasmic Reticulum 0.1898 0.1234 0.1425 0.246 0.2512 0.3223 0.256 0.145 0.1708 0.2269 0.211 0.3083 0.2049 0.1024 0.09 0.204 0.2514 0.2052
Endosome 0.0177 0.0009 0.0122 0.0031 0.0232 0.0045 0.0214 0.0005 0.0099 0.0022 0.0173 0.0057 0.0226 0.0008 0.0023 0.0061 0.0015 0.007
Golgi 0.007 0.0037 0.0103 0.0059 0.0277 0.0091 0.0182 0.0027 0.0132 0.0054 0.0379 0.0104 0.0178 0.0023 0.0043 0.019 0.0052 0.0096
Lipid Particles 0.0111 0.0263 0.0107 0.0097 0.0067 0.0085 0.0233 0.0177 0.0297 0.0126 0.0699 0.0135 0.0236 0.0295 0.0074 0.0095 0.0053 0.0085
Mitochondria 0.0056 0.0001 0.0039 0.0139 0.0207 0.0033 0.015 0.0001 0.012 0.0002 0.0025 0.0008 0.0042 0.0001 0.001 0.0008 0.001 0.0009
None 0.0034 0.0002 0.0025 0.0008 0.0026 0.0003 0.0028 0.0002 0.004 0.0002 0.0006 0.0008 0.0016 0.0003 0.0003 0.0006 0.0008 0.0003
Nuclear Periphery 0.0139 0.0045 0.0074 0.0093 0.0042 0.0036 0.0109 0.0061 0.0066 0.0076 0.0054 0.0033 0.019 0.0042 0.0044 0.009 0.0052 0.0047
Nucleolus 0.0004 0.0002 0.0003 0.0003 0.0003 0.0002 0.0007 0.0002 0.0002 0.0001 0.0001 0.0002 0.0019 0.0002 0.0002 0.0002 0.0001 0.0001
Nucleus 0.0031 0.001 0.0017 0.0017 0.0014 0.001 0.0031 0.0011 0.0012 0.0012 0.0007 0.0011 0.007 0.0013 0.0013 0.0023 0.0008 0.0012
Peroxisomes 0.0016 0.0002 0.0012 0.002 0.0018 0.0007 0.0062 0.0001 0.0022 0.0001 0.002 0.0001 0.0006 0.0001 0.0001 0.0003 0 0.0001
Punctate Nuclear 0.0031 0.0003 0.0009 0.0002 0.0007 0.0003 0.005 0.0002 0.0011 0.0002 0.0005 0.0002 0.0031 0.0004 0.0002 0.0005 0.0001 0.0002
Vacuole 0.0129 0.0049 0.0104 0.014 0.0046 0.0039 0.0139 0.0063 0.0102 0.0155 0.0066 0.0066 0.0122 0.007 0.0095 0.0133 0.0049 0.0054
Vacuole Periphery 0.0018 0.0001 0.0009 0.001 0.0014 0.0003 0.0016 0.0001 0.0009 0.0004 0.0008 0.0006 0.0019 0.0001 0.0006 0.0006 0.0003 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 236.4438 166.7445 259.6622 291.0207 217.998 338.7152 275.4514 363.0075 426.6593 337.449
Translational Efficiency 1.8091 2.1233 1.6063 1.4341 1.8049 1.8355 1.9949 1.504 1.7037 1.8001

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1600 897 1101 11 375 1276 2364 2111 1975 2173 3465 2122

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1720.86 1508.71 2406.15 2158.47 1993.30 1875.24 2445.01 2250.77 1772.59 1723.94 2432.66 2250.29
Standard Deviation 385.61 285.65 450.30 316.43 466.36 364.61 440.82 465.42 416.14 379.88 444.22 464.82
Intensity Change Log 2 -0.189814 0.483597 0.326879 -0.088084 0.294682 0.175260 -0.134328 0.385294 0.247494

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.007554 0.007633 0.030073 0.002726 0.003015 0.011757 0.004596 0.008995 0.006692 0.010055 0.012691 0.008962
Bud Neck 0.009669 0.009306 0.032103 0.012653 0.002902 0.012269 0.008321 0.019499 0.008384 0.011046 0.015878 0.019464
Bud Site 0.095019 0.200520* 0.183348* 0.017093 0.030422 0.102409* 0.037477 0.094727 0.082754 0.142909* 0.083828 0.094325
Cell Periphery 0.315621* 0.465350* 0.188119* 0.181213* 0.361952* 0.374423* 0.460303* 0.230501* 0.324418* 0.411957* 0.373817* 0.230246*
Cytoplasm 0.086012 0.241068* 0.055147 0.001331 0.009137 0.067125 0.121510* 0.095618 0.071415 0.138927* 0.100423* 0.095130
Cytoplasmic Foci 0.029367 0.010374 0.015434 0.001434 0.011371 0.013725 0.009800 0.012791 0.025950 0.012341 0.011590 0.012732
Eisosomes 0.001724 0.001118 0.001759 0.000883 0.000486 0.001983 0.000985 0.001019 0.001489 0.001626 0.001231 0.001018
Endoplasmic Reticulum 0.248058* 0.019303 0.074473 0.336915* 0.326288* 0.229888* 0.122543* 0.167445* 0.262912* 0.142960* 0.107269* 0.168323*
Endosome 0.017060 0.001612 0.019528 0.021754 0.013453 0.012416 0.005630 0.014179 0.016375 0.007956 0.010046 0.014219
Golgi 0.025918 0.003446 0.028938 0.036086 0.100723 0.014971 0.006064 0.014117 0.040121 0.010213 0.013332 0.014231
Lipid Particles 0.110079* 0.017654 0.296895* 0.333169* 0.038354 0.122749* 0.129207* 0.262125* 0.096460 0.079367 0.182490* 0.262493*
Mitochondria 0.002452 0.002845 0.000760 0.000058 0.003022 0.001711 0.004266 0.002456 0.002560 0.002179 0.003152 0.002443
Mitotic Spindle 0.001350 0.001302 0.002388 0.000987 0.001541 0.001454 0.001251 0.001255 0.001386 0.001391 0.001612 0.001254
None 0.000619 0.000297 0.000253 0.000076 0.000479 0.000338 0.000377 0.000547 0.000592 0.000322 0.000338 0.000544
Nuclear Periphery 0.001439 0.000485 0.000810 0.001904 0.001683 0.001371 0.003639 0.001489 0.001485 0.001005 0.002740 0.001491
Nuclear Periphery Foci 0.004682 0.001208 0.004962 0.004181 0.003530 0.002409 0.002141 0.004087 0.004464 0.001913 0.003037 0.004087
Nucleolus 0.000085 0.000058 0.000039 0.000007 0.000100 0.000048 0.000227 0.000105 0.000088 0.000052 0.000167 0.000104
Nucleus 0.001173 0.002162 0.001797 0.000174 0.000505 0.001132 0.002302 0.002232 0.001046 0.001557 0.002141 0.002221
Peroxisomes 0.010019 0.003122 0.002055 0.000260 0.000964 0.007647 0.029944 0.009196 0.008299 0.005779 0.021082 0.009150
Vacuole 0.022099 0.008304 0.057000 0.041111 0.023389 0.016257 0.042927 0.052266 0.022344 0.012974 0.047399 0.052209
Vacuole Periphery 0.010003 0.002832 0.004117 0.005982 0.066685 0.003920 0.006491 0.005351 0.020766 0.003471 0.005737 0.005355

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin NaN NaN NaN NaN NaN -8.11 -2.18 -7.52 2.72 -7.50 -4.51 -6.47 -3.46 1.37 3.93
Bud Neck NaN NaN NaN NaN NaN -11.27 -7.75 -14.58 -5.62 -9.11 -3.35 -8.43 -9.26 -7.03 -2.87
Bud Site NaN NaN NaN NaN NaN -12.50 -1.97 -13.41 1.09 -13.54 -10.42 -0.45 -2.44 8.03 -2.22
Cell Periphery NaN NaN NaN NaN NaN -0.79 -6.75 9.27 15.40 30.02* -9.52 -5.98 11.65 21.81 20.11
Cytoplasm NaN NaN NaN NaN NaN -14.23 -25.92 -21.03 -5.56 4.66 -12.01 -6.55 -4.84 7.42 1.10
Cytoplasmic Foci NaN NaN NaN NaN NaN -1.45 1.23 -0.99 0.69 -3.05 7.88 9.15 7.93 -0.24 -1.06
Eisosomes NaN NaN NaN NaN NaN -10.10 -5.71 -6.27 7.07 -0.46 -0.86 1.81 3.31 6.39 2.91
Endoplasmic Reticulum NaN NaN NaN NaN NaN 5.93 13.55 10.36 7.22 -7.12 15.26 22.85 11.96 -3.54 -10.53
Endosome NaN NaN NaN NaN NaN 0.99 9.14 -0.76 -2.25 -16.69 11.09 8.72 2.61 -10.02 -7.16
Golgi NaN NaN NaN NaN NaN 14.07 15.80 14.36 0.58 -8.39 15.04 13.63 12.78 -3.55 -0.88
Lipid Particles NaN NaN NaN NaN NaN -12.27 -15.67 -31.07* -18.07 -19.64 3.44 -15.96 -24.79 -27.79 -11.64
Mitochondria NaN NaN NaN NaN NaN 2.72 -2.29 0.89 -1.45 3.16 0.77 -1.92 0.24 -0.33 1.36
Mitotic Spindle NaN NaN NaN NaN NaN 0.39 3.04 1.19 0.59 -0.38 -0.27 -2.50 0.43 0.53 1.86
None NaN NaN NaN NaN NaN 0.91 1.98 -0.42 -0.86 -0.84 1.89 2.26 0.07 -0.99 -1.00
Nuclear Periphery NaN NaN NaN NaN NaN 2.55 -12.64 1.54 -1.16 15.77 6.27 -12.40 -0.03 -5.39 11.25
Nuclear Periphery Foci NaN NaN NaN NaN NaN 3.65 4.65 -1.77 -7.78 -9.49 5.07 2.73 0.59 -9.80 -4.68
Nucleolus NaN NaN NaN NaN NaN 7.11 -13.45 -0.63 -10.77 15.29 7.00 -13.32 -2.91 -9.08 9.43
Nucleus NaN NaN NaN NaN NaN -9.60 -21.22 -12.29 -7.85 -0.33 -2.59 -12.95 -8.48 -3.02 -0.82
Peroxisomes NaN NaN NaN NaN NaN -9.93 -20.06 -9.96 -1.59 13.20 3.38 -10.79 -0.79 -4.07 9.81
Vacuole NaN NaN NaN NaN NaN 2.85 -7.30 -10.43 -21.95 -5.21 7.42 -15.43 -16.18 -24.99 -2.55
Vacuole Periphery NaN NaN NaN NaN NaN 12.02 11.51 11.74 -3.58 2.50 13.39 11.73 11.97 -4.59 0.95