Standard name | |
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Description | Member of the protein disulfide isomerase (PDI) family; interacts with and inhibits the chaperone activity of Cne1p; MPD1 overexpression in a pdi1 null mutant suppresses defects in Pdi1p functions such as carboxypeptidase Y maturation |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 1 | 3 | 9 | 6 |
Bud | 2 | 0 | 0 | 0 | 0 | 1 | 9 | 5 | 9 | 8 | 7 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 10 | 11 |
Bud Neck | 0 | 0 | 3 | 1 | 5 | 9 | 11 | 12 | 2 | 4 | 1 | 2 | 2 | 2 | 1 | 0 | 0 | 0 | 3 | 1 | 1 | 2 | 1 | 4 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 0 | 2 | 1 | 0 | 2 | 0 | 1 | 1 | 0 | 1 | 0 | 4 | 1 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 6 | 7 |
Cytoplasm | 212 | 172 | 146 | 146 | 307 | 277 | 356 | 367 | 247 | 298 | 244 | 235 | 369 | 537 | 557 | 150 | 93 | 148 | 158 | 117 | 97 | 191 | 328 | 298 |
Endoplasmic Reticulum | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 10 | 2 | 8 | 2 | 0 | 0 | 0 | 2 | 3 |
Endosome | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 9 | 9 | 6 | 2 | 1 | 2 | 0 | 2 | 5 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 |
Mitochondria | 23 | 3 | 2 | 4 | 1 | 9 | 30 | 19 | 48 | 37 | 60 | 25 | 0 | 0 | 0 | 4 | 8 | 6 | 37 | 13 | 16 | 16 | 31 | 32 |
Nucleus | 0 | 1 | 0 | 1 | 3 | 2 | 3 | 8 | 4 | 5 | 5 | 9 | 1 | 0 | 2 | 20 | 14 | 9 | 0 | 0 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
SpindlePole | 2 | 0 | 1 | 0 | 3 | 3 | 4 | 2 | 1 | 2 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 0 | 2 | 0 | 0 | 3 | 8 | 8 |
Vac/Vac Membrane | 1 | 2 | 0 | 0 | 0 | 3 | 5 | 5 | 10 | 16 | 23 | 29 | 1 | 0 | 0 | 118 | 83 | 65 | 23 | 35 | 14 | 13 | 18 | 24 |
Unique Cell Count | 223 | 173 | 146 | 151 | 312 | 283 | 381 | 386 | 282 | 333 | 289 | 283 | 370 | 537 | 559 | 251 | 163 | 201 | 244 | 176 | 143 | 243 | 433 | 417 |
Labelled Cell Count | 243 | 178 | 154 | 156 | 320 | 307 | 419 | 421 | 324 | 373 | 342 | 308 | 377 | 540 | 562 | 312 | 211 | 244 | 244 | 176 | 143 | 243 | 433 | 417 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 2.9 | 4.1 | 4.0 | 4.3 | 4.7 | 4.0 | 3.7 | 4.2 | 3.6 | 3.7 | 3.5 | 4.2 | 4.6 | 4.9 | 4.9 | 6.6 | 6.7 | 6.3 | 4.3 | 4.9 | 5.4 |
Std Deviation (1e-4) | 0.4 | 0.9 | 0.9 | 1.9 | 1.7 | 1.6 | 1.3 | 1.5 | 1.5 | 1.1 | 1.0 | 1.2 | 0.9 | 1.3 | 1.2 | 1.7 | 1.8 | 1.7 | 1.1 | 1.6 | 1.9 |
Intensity Change (Log2) | – | – | – | 0.11 | 0.24 | -0.01 | -0.11 | 0.07 | -0.14 | -0.1 | -0.2 | 0.07 | 0.2 | 0.29 | 0.3 | 0.74 | 0.74 | 0.66 | 0.12 | 0.29 | 0.43 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 9.9 | 10.1 | 7.6 |