Standard name | |
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Human Ortholog | |
Description | Member of the protein disulfide isomerase (PDI) family; interacts with and inhibits the chaperone activity of Cne1p; MPD1 overexpression in a pdi1 null mutant suppresses defects in Pdi1p functions such as carboxypeptidase Y maturation |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 1 | 3 | 9 | 6 |
Bud | 2 | 0 | 0 | 0 | 0 | 1 | 9 | 5 | 9 | 8 | 7 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 10 | 11 |
Bud Neck | 0 | 0 | 3 | 1 | 5 | 9 | 11 | 12 | 2 | 4 | 1 | 2 | 2 | 2 | 1 | 0 | 0 | 0 | 3 | 1 | 1 | 2 | 1 | 4 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 0 | 2 | 1 | 0 | 2 | 0 | 1 | 1 | 0 | 1 | 0 | 4 | 1 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 6 | 7 |
Cytoplasm | 212 | 172 | 146 | 146 | 307 | 277 | 356 | 367 | 247 | 298 | 244 | 235 | 369 | 537 | 557 | 150 | 93 | 148 | 158 | 117 | 97 | 191 | 328 | 298 |
Endoplasmic Reticulum | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 10 | 2 | 8 | 2 | 0 | 0 | 0 | 2 | 3 |
Endosome | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 9 | 9 | 6 | 2 | 1 | 2 | 0 | 2 | 5 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 |
Mitochondria | 23 | 3 | 2 | 4 | 1 | 9 | 30 | 19 | 48 | 37 | 60 | 25 | 0 | 0 | 0 | 4 | 8 | 6 | 37 | 13 | 16 | 16 | 31 | 32 |
Nucleus | 0 | 1 | 0 | 1 | 3 | 2 | 3 | 8 | 4 | 5 | 5 | 9 | 1 | 0 | 2 | 20 | 14 | 9 | 0 | 0 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
SpindlePole | 2 | 0 | 1 | 0 | 3 | 3 | 4 | 2 | 1 | 2 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 0 | 2 | 0 | 0 | 3 | 8 | 8 |
Vac/Vac Membrane | 1 | 2 | 0 | 0 | 0 | 3 | 5 | 5 | 10 | 16 | 23 | 29 | 1 | 0 | 0 | 118 | 83 | 65 | 23 | 35 | 14 | 13 | 18 | 24 |
Unique Cell Count | 223 | 173 | 146 | 151 | 312 | 283 | 381 | 386 | 282 | 333 | 289 | 283 | 370 | 537 | 559 | 251 | 163 | 201 | 244 | 176 | 143 | 243 | 433 | 417 |
Labelled Cell Count | 243 | 178 | 154 | 156 | 320 | 307 | 419 | 421 | 324 | 373 | 342 | 308 | 377 | 540 | 562 | 312 | 211 | 244 | 244 | 176 | 143 | 243 | 433 | 417 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 2.9 | 4.1 | 4.0 | 4.3 | 4.7 | 4.0 | 3.7 | 4.2 | 3.6 | 3.7 | 3.5 | 4.2 | 4.6 | 4.9 | 4.9 | 6.6 | 6.7 | 6.3 | 4.3 | 4.9 | 5.4 |
Std Deviation (1e-4) | 0.4 | 0.9 | 0.9 | 1.9 | 1.7 | 1.6 | 1.3 | 1.5 | 1.5 | 1.1 | 1.0 | 1.2 | 0.9 | 1.3 | 1.2 | 1.7 | 1.8 | 1.7 | 1.1 | 1.6 | 1.9 |
Intensity Change (Log2) | – | – | – | 0.11 | 0.24 | -0.01 | -0.11 | 0.07 | -0.14 | -0.1 | -0.2 | 0.07 | 0.2 | 0.29 | 0.3 | 0.74 | 0.74 | 0.66 | 0.12 | 0.29 | 0.43 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 9.9 | 10.1 | 7.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -1.2604 | -0.6704 | -0.9287 | -0.9608 | -0.9975 | -0.7999 | -0.8054 | -0.6657 | -0.6924 | -0.9621 | -0.8077 | -0.7144 | -1.3947 | -1.1561 | -1.2463 | -0.7297 | -0.8577 | -1.3267 |
Actin | 0.0037 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0225 | 0.0001 | 0.0095 | 0.0003 | 0.0027 | 0.0013 | 0.0181 | 0.0021 | 0.0066 | 0.0423 | 0.0296 | 0.0004 |
Bud | 0.0065 | 0.0094 | 0.0044 | 0 | 0.0185 | 0.0017 | 0.0048 | 0.0002 | 0.0015 | 0.0002 | 0.0009 | 0.0029 | 0.0018 | 0.0068 | 0.0055 | 0.0074 | 0.0379 | 0.0016 |
Bud Neck | 0.0012 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0025 | 0.0004 | 0.0012 | 0.0005 | 0.0004 | 0.0023 | 0.0114 | 0.0004 | 0.0009 | 0.0014 | 0.0008 | 0.0007 |
Bud Periphery | 0.0039 | 0.0022 | 0.0005 | 0 | 0.0046 | 0.0016 | 0.0105 | 0.0004 | 0.0029 | 0.0003 | 0.0015 | 0.0026 | 0.0042 | 0.0041 | 0.0023 | 0.0137 | 0.0158 | 0.0015 |
Bud Site | 0.0051 | 0.0106 | 0.0019 | 0 | 0.0052 | 0.0001 | 0.0025 | 0.0011 | 0.0046 | 0.0055 | 0.0004 | 0.0005 | 0.0054 | 0.0105 | 0.0083 | 0.0067 | 0.0106 | 0.0004 |
Cell Periphery | 0.0005 | 0.0004 | 0.0001 | 0 | 0.0004 | 0 | 0.0005 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0004 | 0.0005 | 0.0004 | 0.0003 | 0.0006 | 0.002 | 0.0002 |
Cytoplasm | 0.0164 | 0.0364 | 0.0438 | 0.0109 | 0.0311 | 0.0299 | 0.0332 | 0.058 | 0.0376 | 0.026 | 0.0489 | 0.0605 | 0.0699 | 0.1405 | 0.1031 | 0.0342 | 0.109 | 0.1082 |
Cytoplasmic Foci | 0.0065 | 0.0043 | 0.006 | 0.0034 | 0.0056 | 0.0114 | 0.0131 | 0.0148 | 0.0179 | 0.0338 | 0.0237 | 0.0113 | 0.0233 | 0.0272 | 0.0178 | 0.0446 | 0.0517 | 0.0297 |
Eisosomes | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0003 | 0.0005 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.0009 | 0.0005 | 0.001 | 0.0002 | 0.0002 | 0.0003 | 0.0012 | 0.0005 | 0.0012 | 0.0005 | 0.0016 | 0.0007 | 0.0029 | 0.0017 | 0.0021 | 0.0032 | 0.0032 | 0.0009 |
Endosome | 0.004 | 0.0042 | 0.0048 | 0.0002 | 0.0002 | 0.002 | 0.0097 | 0.0038 | 0.0206 | 0.0135 | 0.0345 | 0.0083 | 0.0182 | 0.0354 | 0.0187 | 0.0576 | 0.1097 | 0.0225 |
Golgi | 0.0011 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0036 | 0.0002 | 0.0014 | 0.0005 | 0.002 | 0.0007 | 0.005 | 0.0008 | 0.0044 | 0.006 | 0.0141 | 0.0003 |
Lipid Particles | 0.0014 | 0.0007 | 0.0004 | 0.0002 | 0.0002 | 0.0003 | 0.0079 | 0.0003 | 0.0024 | 0.001 | 0.0234 | 0.0012 | 0.0085 | 0.0017 | 0.0042 | 0.0163 | 0.0776 | 0.0022 |
Mitochondria | 0.0009 | 0.0005 | 0.0007 | 0.0003 | 0.0013 | 0.0003 | 0.0029 | 0.0006 | 0.0077 | 0.0006 | 0.0021 | 0.0016 | 0.0052 | 0.001 | 0.0063 | 0.0221 | 0.007 | 0.001 |
None | 0.9357 | 0.9122 | 0.927 | 0.9832 | 0.9273 | 0.9475 | 0.85 | 0.9121 | 0.8722 | 0.9083 | 0.8128 | 0.8718 | 0.7874 | 0.7078 | 0.7805 | 0.6227 | 0.3922 | 0.7923 |
Nuclear Periphery | 0.0022 | 0.0009 | 0.0004 | 0.0001 | 0.0002 | 0.0003 | 0.0043 | 0.0002 | 0.0028 | 0.0004 | 0.0049 | 0.0016 | 0.0058 | 0.0013 | 0.0056 | 0.0203 | 0.0087 | 0.0034 |
Nucleolus | 0.001 | 0.0004 | 0.0006 | 0.0002 | 0.0008 | 0.0003 | 0.0027 | 0.0009 | 0.0007 | 0.0006 | 0.0015 | 0.0009 | 0.0012 | 0.0016 | 0.0013 | 0.0042 | 0.0013 | 0.0013 |
Nucleus | 0.0022 | 0.0034 | 0.0028 | 0.0002 | 0.0014 | 0.0016 | 0.0046 | 0.0018 | 0.0017 | 0.0012 | 0.0058 | 0.0034 | 0.007 | 0.0041 | 0.0038 | 0.0106 | 0.0032 | 0.0026 |
Peroxisomes | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0004 | 0.0122 | 0.0002 | 0.0029 | 0.001 | 0.0044 | 0.0005 | 0.0059 | 0.001 | 0.0009 | 0.0045 | 0.0119 | 0.0007 |
Punctate Nuclear | 0.0031 | 0.0007 | 0.0008 | 0.0002 | 0.0016 | 0.001 | 0.0078 | 0.0008 | 0.0026 | 0.001 | 0.0071 | 0.0082 | 0.0019 | 0.006 | 0.0035 | 0.034 | 0.0075 | 0.01 |
Vacuole | 0.0025 | 0.0115 | 0.0038 | 0.0002 | 0.0006 | 0.0006 | 0.0026 | 0.0032 | 0.0064 | 0.0042 | 0.0191 | 0.0188 | 0.0148 | 0.0436 | 0.0206 | 0.0373 | 0.096 | 0.0183 |
Vacuole Periphery | 0.0003 | 0.0009 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0006 | 0.0002 | 0.0016 | 0.0005 | 0.0021 | 0.0006 | 0.0014 | 0.0019 | 0.0028 | 0.0096 | 0.0099 | 0.0016 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 21.3302 | 18.5525 | 7.6482 | 19.5258 | 32.1402 | 19.5899 | 14.5899 | 14.7708 | 16.1548 | 26.6648 |
Translational Efficiency | 1.9715 | 0.9266 | 1.6913 | 1.0237 | 0.8817 | 2.2633 | 1.1889 | 1.029 | 1.2179 | 1.2614 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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