Standard name
Description Member of the protein disulfide isomerase (PDI) family; interacts with and inhibits the chaperone activity of Cne1p; MPD1 overexpression in a pdi1 null mutant suppresses defects in Pdi1p functions such as carboxypeptidase Y maturation

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.95 0.99 1.0 0.97 0.98 0.98 0.93 0.95 0.88 0.89 0.84 0.83 1.0 1.0 1.0 0.6 0.57 0.74 0.65 0.67 0.68 0.79 0.76 0.72
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0 0 0 0 0 0.08 0.05 0.17 0.11 0.21 0.09 0 0 0 0 0.05 0 0.15 0.07 0.12 0.07 0.07 0.08
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.09 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0.05 0.08 0.1 0 0 0 0.47 0.51 0.32 0.1 0.2 0.1 0.06 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 3 1 1 3 9 6
Bud 2 0 0 0 0 1 9 5 9 8 7 4 0 0 0 0 0 0 1 0 1 1 10 11
Bud Neck 0 0 3 1 5 9 11 12 2 4 1 2 2 2 1 0 0 0 3 1 1 2 1 4
Bud Site 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cell Periphery 2 0 2 1 0 2 0 1 1 0 1 0 4 1 1 0 0 1 1 0 0 1 6 7
Cytoplasm 212 172 146 146 307 277 356 367 247 298 244 235 369 537 557 150 93 148 158 117 97 191 328 298
Endoplasmic Reticulum 1 0 0 0 0 0 0 0 0 0 0 2 0 0 0 10 2 8 2 0 0 0 2 3
Endosome 0 0 0 1 0 0 0 1 0 2 0 0 0 0 0 9 9 6 2 1 2 0 2 5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 2
Mitochondria 23 3 2 4 1 9 30 19 48 37 60 25 0 0 0 4 8 6 37 13 16 16 31 32
Nucleus 0 1 0 1 3 2 3 8 4 5 5 9 1 0 2 20 14 9 0 0 0 1 0 0
Nuclear Periphery 0 0 0 1 1 0 0 0 0 1 1 1 0 0 0 0 0 0 0 0 0 0 1 1
Nucleolus 0 0 0 1 0 0 1 1 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1
SpindlePole 2 0 1 0 3 3 4 2 1 2 0 1 0 0 1 0 2 0 2 0 0 3 8 8
Vac/Vac Membrane 1 2 0 0 0 3 5 5 10 16 23 29 1 0 0 118 83 65 23 35 14 13 18 24
Unique Cell Count 223 173 146 151 312 283 381 386 282 333 289 283 370 537 559 251 163 201 244 176 143 243 433 417
Labelled Cell Count 243 178 154 156 320 307 419 421 324 373 342 308 377 540 562 312 211 244 244 176 143 243 433 417


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 2.9 4.1 4.0 4.3 4.7 4.0 3.7 4.2 3.6 3.7 3.5 4.2 4.6 4.9 4.9 6.6 6.7 6.3 4.3 4.9 5.4
Std Deviation (1e-4) 0.4 0.9 0.9 1.9 1.7 1.6 1.3 1.5 1.5 1.1 1.0 1.2 0.9 1.3 1.2 1.7 1.8 1.7 1.1 1.6 1.9
Intensity Change (Log2) 0.11 0.24 -0.01 -0.11 0.07 -0.14 -0.1 -0.2 0.07 0.2 0.29 0.3 0.74 0.74 0.66 0.12 0.29 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 9.9 10.1 7.6