ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YOR292C is not an essential gene

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.99 0.99 0.99 0.98 0.98 0.96 0.9 0.91 0.87 0.89 1.0 0.99 0.99 0.55 0.54 0.52 0.69 0.79 0.73 0.78 0.74 0.67
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.11 0 0.12 0.07 0 0 0 0 0 0 0.05 0 0.08 0 0.05 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.13 0.12 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0.05 0.08 0.07 0.08 0 0 0 0.54 0.65 0.53 0.16 0.08 0.1 0.11 0.11 0.17
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 1 1 3 1 0 0 3
Bud 0 0 0 2 0 3 2 2 13 1 2 2 0 0 1 0 0 0 0 4 2 4 3 5
Bud Neck 0 1 11 4 13 9 13 21 5 13 3 5 0 0 2 0 0 0 0 3 4 2 0 3
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1 1 2 1 2 0 0 0 0 1 0 0 0 0 0 0 0 0 3 4 0 2 1
Cytoplasm 166 534 357 341 363 623 733 800 553 643 453 543 317 335 410 276 224 77 132 414 238 170 203 234
Endoplasmic Reticulum 0 1 2 0 0 1 0 0 0 0 0 1 0 1 1 27 13 11 0 1 3 0 1 2
Endosome 0 1 1 0 0 2 1 1 1 2 4 1 1 1 2 22 20 2 6 7 2 1 5 4
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 2 1 0 2 1 0 0 0 1
Mitochondria 5 5 0 0 2 3 5 5 67 26 63 40 0 0 0 22 5 4 10 23 27 3 12 16
Nucleus 0 0 0 0 2 3 6 4 7 7 6 13 0 0 1 45 53 18 0 0 0 1 1 0
Nuclear Periphery 0 1 0 0 0 0 1 1 0 1 0 0 0 0 0 0 2 0 1 1 0 0 1 0
Nucleolus 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 1 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 1 0 0 1 0 0 0 0
SpindlePole 0 2 1 2 0 4 4 9 2 7 0 6 0 0 2 0 1 0 1 7 1 1 4 6
Vac/Vac Membrane 3 2 1 2 2 15 20 30 30 56 36 52 1 4 1 274 270 78 30 41 31 23 30 58
Unique Cell Count 172 543 362 344 366 637 749 832 612 710 519 613 318 340 416 505 417 148 192 526 327 219 273 347
Labelled Cell Count 175 548 375 353 383 666 785 874 678 757 568 663 319 342 421 669 590 191 192 526 327 219 273 347


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 3.5 4.6 4.4 4.1 4.0 4.1 4.3 3.5 4.0 3.6 3.9 4.4 4.8 4.4 4.9 6.3 5.7 4.8 5.1 5.0
Std Deviation (1e-4) 0.9 0.6 0.9 1.3 1.3 1.0 1.1 1.0 1.1 1.0 0.8 0.8 0.9 1.3 1.1 1.3 1.8 1.6 1.4 1.5 1.4
Intensity Change (Log2) -0.08 -0.15 -0.2 -0.17 -0.09 -0.41 -0.2 -0.34 -0.25 -0.08 0.05 -0.05 0.09 0.45 0.31 0.07 0.15 0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 16.8 18.8 14.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.5122 1.3058 1.5899 1.0349 1.7042 1.5414 4.3852 4.8759 4.5477 4.7071 3.8942 4.2795 0.993 1.7288 1.5213 2.1426 1.9613 2.0125
Actin 0.0499 0.0002 0.0257 0.0012 0.0456 0.0176 0.0334 0.0028 0.0065 0.0002 0.003 0.0039 0.0073 0 0.0001 0.0004 0 0.0001
Bud 0.0009 0 0.001 0.0006 0.0069 0.0005 0.001 0.0017 0.0036 0 0.0089 0.0008 0.0001 0 0.0094 0.0004 0 0
Bud Neck 0.0029 0 0.0004 0.0002 0.0012 0.0141 0.0021 0.0001 0.0002 0.0001 0.0001 0.0002 0.0008 0 0 0 0 0.0052
Bud Periphery 0.0017 0.0001 0.0035 0.0018 0.0186 0.0012 0.0021 0.004 0.0052 0.0001 0.0227 0.0016 0.0001 0.0001 0.0009 0.0009 0 0
Bud Site 0.0126 0.0002 0.002 0.0026 0.0132 0.0002 0.0134 0.0029 0.0015 0.0001 0.0024 0.0001 0.0007 0 0.0003 0.0002 0 0.0001
Cell Periphery 0.0008 0.0001 0.0004 0.0003 0.0005 0.0001 0.0006 0.0004 0.0002 0.0001 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0001
Cytoplasm 0.0244 0.0711 0.0384 0.0424 0.0257 0.0714 0.0376 0.0731 0.0466 0.0256 0.0588 0.089 0.028 0.02 0.0289 0.0206 0.0868 0.0397
Cytoplasmic Foci 0.0188 0.0025 0.0048 0.0201 0.0339 0.0071 0.0092 0.0141 0.0081 0.0076 0.0083 0.0096 0.0063 0.0018 0.0019 0.0072 0.0027 0.0034
Eisosomes 0.0011 0.0004 0.0009 0.0002 0.0004 0.0002 0.0011 0.0003 0.0003 0.0001 0.0001 0.0001 0.0003 0.0002 0.0002 0.0004 0.0001 0.0001
Endoplasmic Reticulum 0.004 0.0008 0.0007 0.0005 0.0059 0.0005 0.0044 0.0011 0.0012 0.0007 0.0005 0.0015 0.0022 0.0003 0.0003 0.0005 0.0001 0.0003
Endosome 0.0161 0.0008 0.0049 0.0041 0.0213 0.0035 0.0139 0.0021 0.0036 0.0354 0.0063 0.0021 0.004 0.0006 0.0003 0.0004 0.0003 0.0011
Golgi 0.0073 0.0001 0.0102 0.0012 0.0185 0.006 0.0079 0.0005 0.0013 0.0187 0.0047 0.001 0.0006 0 0 0.0002 0 0
Lipid Particles 0.0186 0.0005 0.0064 0.0026 0.0058 0.0047 0.0069 0.0015 0.0046 0.0239 0.0011 0.0022 0.0008 0.0002 0.0002 0.0012 0 0.0005
Mitochondria 0.0372 0.0002 0.0222 0.0109 0.0508 0.0024 0.0211 0.0014 0.0024 0.0034 0.0105 0.0006 0.0002 0.0002 0.0005 0.0022 0 0.0001
None 0.7501 0.9211 0.8555 0.8833 0.6303 0.8586 0.7777 0.8844 0.8652 0.8758 0.8583 0.8681 0.9415 0.9751 0.9553 0.9607 0.909 0.9478
Nuclear Periphery 0.0054 0.0003 0.0005 0.0003 0.0303 0.0003 0.032 0.0006 0.0033 0.0004 0.0003 0.0025 0.0016 0.0002 0.0001 0.0005 0.0001 0.0001
Nucleolus 0.0027 0.0001 0.0002 0.0004 0.0004 0.0005 0.0007 0.0004 0.0052 0.0001 0.0002 0.0002 0.0002 0.0001 0.0001 0.0005 0 0.0001
Nucleus 0.0031 0.0003 0.0004 0.0005 0.003 0.001 0.0158 0.0006 0.006 0.0003 0.0005 0.0011 0.0013 0.0002 0.0006 0.0004 0.0002 0.0003
Peroxisomes 0.0139 0.0001 0.0179 0.0105 0.0783 0.0069 0.011 0.0034 0.016 0.0005 0.0113 0.0049 0.0006 0 0 0.0016 0 0
Punctate Nuclear 0.0149 0.0002 0.0005 0.0129 0.0017 0.0025 0.0024 0.0022 0.0166 0.0002 0.0007 0.0093 0.003 0.0002 0.0001 0.0012 0.0002 0.0003
Vacuole 0.01 0.0009 0.0031 0.0025 0.0031 0.0004 0.0027 0.0018 0.0018 0.0054 0.0007 0.001 0.0003 0.0005 0.0007 0.0004 0.0003 0.0005
Vacuole Periphery 0.0034 0.0001 0.0003 0.0008 0.0046 0.0001 0.003 0.0003 0.0005 0.0013 0.0002 0.0004 0.0001 0.0001 0.0001 0.0002 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.4578 7.4243 5.5092 10.5527 14.6138 8.3006 11.7963 14.4758 13.7902 14.1295
Translational Efficiency 0.6613 0.8121 1.0344 0.5056 0.4423 1.2968 0.7219 0.6733 0.6855 0.6514

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1892 685 2551 1579 2283 1915 2597 1964 4175 2600 5148 3543

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 686.64 714.44 719.33 761.32 637.99 782.62 744.25 779.10 660.04 764.66 731.90 771.18
Standard Deviation 72.00 83.74 87.81 114.39 70.74 99.14 92.22 110.58 75.31 99.94 90.92 112.64
Intensity Change Log 2 0.057259 0.067100 0.148949 0.294778 0.222254 0.288275 0.176543 0.143912 0.217734

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000174 0.000825 0.000107 0.000560 0.000136 0.000858 0.000164 0.000579 0.000153 0.000849 0.000136 0.000570
Bud Neck 0.018775 0.051089 0.008217 0.008329 0.021891 0.054897 0.010109 0.012427 0.020479 0.053894 0.009172 0.010601
Bud Site 0.004353 0.020453 0.017716 0.030600 0.005323 0.024165 0.015773 0.031813 0.004884 0.023187 0.016736 0.031272
Cell Periphery 0.000140 0.000268 0.000372 0.000187 0.000117 0.000300 0.000320 0.000130 0.000127 0.000291 0.000346 0.000155
Cytoplasm 0.456008* 0.295667* 0.396462* 0.469121* 0.466990* 0.361672* 0.401097* 0.513933* 0.462013* 0.344282* 0.398800* 0.493962*
Cytoplasmic Foci 0.250270* 0.328210* 0.009189 0.021059 0.220215* 0.246081* 0.010468 0.015795 0.233835* 0.267719* 0.009834 0.018141
Eisosomes 0.000187 0.000280 0.000038 0.000041 0.000172 0.000284 0.000047 0.000031 0.000179 0.000283 0.000043 0.000035
Endoplasmic Reticulum 0.000692 0.001020 0.000860 0.001330 0.000591 0.000840 0.002097 0.001668 0.000636 0.000887 0.001484 0.001517
Endosome 0.016343 0.038501 0.001210 0.005682 0.010261 0.029768 0.001728 0.004124 0.013017 0.032069 0.001471 0.004818
Golgi 0.003038 0.009706 0.000087 0.001590 0.002412 0.009360 0.000191 0.003183 0.002696 0.009451 0.000140 0.002473
Lipid Particles 0.011854 0.011964 0.000727 0.001771 0.008516 0.010788 0.000908 0.001160 0.010028 0.011098 0.000818 0.001432
Mitochondria 0.005480 0.023439 0.000878 0.001385 0.004654 0.024280 0.001252 0.001964 0.005028 0.024059 0.001067 0.001706
Mitotic Spindle 0.003090 0.011389 0.004710 0.032072 0.002424 0.009692 0.011304 0.022408 0.002726 0.010139 0.008036 0.026715
None 0.001490 0.001632 0.010065 0.010653 0.002688 0.003283 0.006281 0.005295 0.002145 0.002848 0.008156 0.007683
Nuclear Periphery 0.000510 0.000496 0.001380 0.002059 0.000459 0.000483 0.002143 0.002126 0.000482 0.000486 0.001765 0.002096
Nuclear Periphery Foci 0.000274 0.000554 0.000176 0.001111 0.000193 0.000399 0.000338 0.000898 0.000230 0.000440 0.000258 0.000993
Nucleolus 0.001244 0.001867 0.000887 0.001224 0.001130 0.002049 0.000642 0.000630 0.001182 0.002001 0.000764 0.000895
Nucleus 0.158447* 0.099237 0.525973* 0.312845* 0.200772* 0.125529 0.495315* 0.298264* 0.181592* 0.118602 0.510507* 0.304762*
Peroxisomes 0.008066 0.030995 0.000298 0.001687 0.007253 0.020469 0.000316 0.000842 0.007621 0.023242 0.000307 0.001219
Vacuole 0.058584 0.069359 0.020360 0.094782 0.043157 0.071993 0.039229 0.081926 0.050148 0.071299 0.029879 0.087655
Vacuole Periphery 0.000981 0.003050 0.000287 0.001913 0.000645 0.002811 0.000277 0.000805 0.000797 0.002874 0.000282 0.001299

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.37 1.07 -2.68 2.13 -3.62 -2.52 -2.81 -1.82 1.35 -1.45 -3.21 -0.35 -2.73 1.88 -2.69
Bud Neck -7.29 7.31 7.10 9.91 -0.23 -11.24 7.34 5.58 15.29 -1.66 -13.87 10.39 8.62 18.78 -1.67
Bud Site -7.32 -6.07 -6.54 0.58 -1.30 -9.17 -5.25 -7.32 1.27 -2.90 -11.40 -7.98 -9.82 1.60 -2.97
Cell Periphery -2.16 -5.72 0.46 2.23 5.54 -7.23 -4.77 0.98 7.75 5.21 -6.84 -7.47 1.02 7.07 7.70
Cytoplasm 13.90 11.16 8.12 -7.09 -1.80 12.58 15.17 5.47 -6.52 -8.36 17.54 18.67 9.43 -8.21 -7.42
Cytoplasmic Foci -7.39 50.07 47.78 33.98 -6.51 -3.65 45.21 43.37 45.39 -5.32 -5.88 66.75 63.87 55.91 -8.29
Eisosomes -1.94 24.32 22.52 4.97 -5.52 -4.34 11.16 11.84 10.74 2.75 -4.70 20.12 19.99 11.53 -1.12
Endoplasmic Reticulum -2.87 -7.14 -12.83 -9.33 -6.99 -1.54 -6.12 -12.95 -9.45 1.17 -2.19 -7.84 -18.18 -12.33 -1.52
Endosome -7.03 14.40 10.38 11.46 -6.87 -11.54 11.99 8.42 16.73 -4.96 -12.62 18.74 13.35 20.16 -8.42
Golgi -5.68 6.41 4.39 8.15 -2.70 -7.80 9.09 1.31 7.31 -2.10 -9.18 10.37 3.39 10.51 -2.81
Lipid Particles -0.09 14.72 13.24 7.85 -5.77 -2.68 14.98 13.92 13.91 -6.12 -1.43 20.84 19.02 15.78 -8.13
Mitochondria -5.70 5.87 5.43 7.26 -2.21 -10.71 6.61 5.92 12.83 -1.73 -11.97 8.74 7.94 14.78 -2.83
Mitotic Spindle -2.78 -1.76 -9.10 -5.09 -8.39 -4.63 -5.95 -7.76 -3.89 -3.12 -5.29 -5.79 -11.93 -6.87 -8.39
None -0.50 -8.31 -6.21 -5.77 1.09 -1.19 -5.02 -3.13 -2.06 1.31 -2.01 -9.47 -6.79 -5.05 1.80
Nuclear Periphery 0.03 -16.76 -15.43 -15.23 -8.55 -0.30 -23.88 -21.03 -20.60 -2.83 -0.12 -29.03 -25.48 -24.94 -8.54
Nuclear Periphery Foci -3.24 -0.08 -4.62 -3.24 -4.55 -1.90 -4.37 -6.32 -4.35 -4.93 -2.58 -2.98 -7.62 -5.37 -6.71
Nucleolus -1.42 1.58 0.25 1.94 -2.34 -3.23 3.45 1.06 3.86 -2.49 -3.33 3.08 0.85 4.14 -3.24
Nucleus 8.35 -56.10 -23.47 -26.79 22.69 13.57 -46.81 -19.25 -29.80 22.30 14.68 -71.94 -29.95 -39.82 31.93
Peroxisomes -8.42 9.35 8.22 11.54 -2.13 -10.35 13.11 12.01 17.05 -2.69 -13.07 15.41 13.87 20.37 -3.13
Vacuole -2.88 11.67 -18.79 -15.03 -24.15 -9.18 -5.02 -20.38 -13.59 -16.73 -8.43 3.50 -27.64 -20.09 -28.91
Vacuole Periphery -4.28 9.15 -0.25 3.68 -3.11 -11.27 7.12 1.48 11.05 -2.32 -10.94 11.48 0.50 9.54 -3.81