Standard name
Human Ortholog
Description Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10A has a paralog, RPS10B, that arose from the whole genome duplication; mutations in the human homolog associated with Diamond-Blackfan anemia

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.08 0.07 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.08 0.1 0.11 0.12 0.09 0.1 0.05 0.06 0.07 0.12 0.16 0.1 0.11 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0 0.1 0.15 0 0 0 0 0 0 0 0 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.07 0 0 0.34 0.21 0.18 0.29 0.62 0.42 0.48 0.52 0 0 0 0.1 0.1 0 0 0 0 0 0 0
Nucleus 0.86 0.74 0.47 0.15 0.38 0.32 0.37 0.36 0.39 0.38 0.49 0.49 0.21 0.21 0.14 0.42 0.48 0.52 0.71 0.64 0.36 0.42 0.41 0.29
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.16 0.28 0.5 0.81 0.39 0.31 0.24 0.22 0.21 0.21 0.24 0.15 0.74 0.66 0.67 0.41 0.3 0.4 0.2 0.27 0.5 0.46 0.32 0.31
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.05 0.21 0.14 0.22 0.18 0.16 0.05 0.11 0.05 0.08 0.06 0.23 0.34 0 0 0 0 0 0 0 0 0.08
Vac/Vac Membrane 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0 0.09 0.1 0.06 0 0 0 0.05 0.13 0.16
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 1 0 2 5 5 6 6 13 11 13 8 0 1 0 0 0 0 0 4 2 2 2 2
Bud Neck 0 0 1 2 0 4 6 6 5 6 1 2 0 5 1 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 1 2 10 3 4 15 14 28 24 0 0 1 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 7 6 7 9 31 33 42 33 34 16 21 14 32 50 13 14 5 0 1 1 0 1 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 2 0 0 0 0 0 0
Endosome 1 2 3 9 0 20 17 14 0 7 2 5 8 26 49 5 5 4 1 5 5 3 5 13
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 1
Mitochondria 1 29 3 6 40 69 53 99 226 148 170 187 2 5 3 13 13 2 0 5 3 0 5 8
Nucleus 77 320 94 31 44 103 111 125 143 134 171 174 46 56 45 57 64 60 82 305 84 82 83 67
Nuclear Periphery 1 6 2 0 0 0 0 4 3 4 1 5 0 0 1 3 3 1 0 0 2 0 1 2
Nucleolus 14 122 99 167 45 102 72 75 77 74 85 53 160 177 214 56 40 46 23 128 117 90 65 71
Peroxisomes 0 0 0 0 0 0 1 0 0 0 0 0 0 0 5 1 1 0 0 0 0 0 0 0
SpindlePole 0 0 9 44 16 73 53 56 19 38 18 29 13 62 110 0 3 5 1 1 7 3 8 19
Vac/Vac Membrane 0 16 1 2 1 14 19 17 6 14 8 7 1 1 5 12 13 7 3 19 6 10 27 38
Unique Cell Count 90 430 198 206 116 325 300 346 364 350 351 358 215 267 319 136 132 116 116 475 233 197 205 232
Labelled Cell Count 97 503 218 271 162 431 374 448 541 484 513 515 244 365 484 162 159 132 116 475 233 197 205 232


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.6 8.8 10.4 9.1 4.8 5.0 5.1 5.3 4.1 4.4 4.5 4.5 11.6 8.5 7.8 8.2 10.8 10.0 12.3 11.7 10.2
Std Deviation (1e-4) 2.7 2.3 2.2 2.8 1.6 1.3 1.4 1.6 1.2 1.1 1.3 1.1 3.5 2.6 2.1 2.7 3.3 4.1 3.1 2.4 2.4
Intensity Change (Log2) -0.19 -1.12 -1.06 -1.02 -0.97 -1.34 -1.22 -1.21 -1.22 0.16 -0.29 -0.41 -0.33 0.05 -0.06 0.24 0.17 -0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 1.9 2.8 3.2 3.6 2.7 2.9 0.9 1.5 1.6 3.5 4.5 2.5 2.8 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 1.7 0 2.5 2.3 1.6 0 0 0 0 1.4 3.6 5.1 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 6.3 5.6 7.8 0 0 0 0 0 0 0 3.4 3.5 0
Nucleus -7.0 -1.6 -3.6 -2.3 -2.6 -1.9 -2.1 0.3 0.3 -5.6 -6.0 -8.3 -1.0 0.2 0.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 6.6 0 -4.2 -6.0 -6.8 0 0 0 0 5.1 3.5 3.9 -1.6 -3.6 -1.8
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 5.0 0 5.5 4.3 4.0 0 0 0 0 0.7 5.5 7.9 0 0 -0.1
Vacuole 0 0 2.5 3.2 2.8 0 2.4 0 0 0 0 0 3.9 4.1 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.2224 6.675 5.6465 6.3309 5.1617 5.8537 10.3083 13.7562 12.4083 11.6889 11.3981 11.9814 9.6889 10.0506 9.5555 8.4164 9.0494 8.8696
Actin 0.0055 0 0.0047 0.0001 0.0109 0 0.0041 0 0.0426 0 0.0347 0 0.0073 0 0.009 0 0.0105 0.0027
Bud 0.0002 0.0005 0.0001 0.0001 0.0006 0 0.0002 0 0.0008 0.0002 0.0012 0.0001 0.0002 0 0.0003 0.0001 0.0004 0.0001
Bud Neck 0.0005 0.0003 0.0002 0.0003 0.001 0.0009 0.0002 0.0001 0.0005 0.0002 0.0006 0.0009 0.0002 0.0001 0.0002 0.0005 0.0004 0.0015
Bud Periphery 0.0007 0.0002 0.0002 0.0001 0.0013 0.0002 0.0005 0.0001 0.0011 0.0003 0.0026 0.0006 0.0002 0.0001 0.0005 0.0002 0.001 0.0005
Bud Site 0.0005 0.0014 0.0002 0.0002 0.0008 0.0001 0.0001 0.0001 0.0016 0.0002 0.0034 0.0001 0.0006 0.0001 0.0018 0.0002 0.0008 0.0002
Cell Periphery 0.0005 0.0001 0.0001 0.0001 0.0004 0.0001 0.0001 0.0001 0.0004 0.0003 0.0003 0.0003 0.0002 0.0002 0.0002 0.0001 0.0003 0.0003
Cytoplasm 0.0013 0.0001 0.0036 0.0008 0.0012 0 0.0004 0.0004 0.004 0 0.0004 0.0001 0.0072 0 0.0004 0 0.0005 0
Cytoplasmic Foci 0.0075 0.0013 0.002 0.0001 0.0031 0 0.0009 0.0001 0.0079 0 0.005 0 0.0263 0 0.0014 0 0.0017 0.0003
Eisosomes 0.0001 0 0 0 0.0001 0 0.0001 0 0.0002 0 0.0004 0 0.0001 0 0 0 0.0002 0.0001
Endoplasmic Reticulum 0.002 0.0001 0.0008 0.0002 0.0069 0.0001 0.0073 0 0.0015 0 0.0007 0 0.0037 0 0.0007 0 0.0006 0.0002
Endosome 0.0078 0.0011 0.003 0.0002 0.0217 0.0001 0.0535 0 0.0047 0 0.0083 0.0003 0.015 0 0.0048 0.0001 0.0075 0.001
Golgi 0.0019 0.0002 0.0014 0 0.0052 0 0.0144 0 0.0042 0 0.0138 0 0.0034 0 0.0017 0 0.0024 0.001
Lipid Particles 0.0125 0.0048 0.0075 0.0001 0.0088 0.0002 0.0038 0.0001 0.0057 0 0.0214 0.0002 0.0176 0.0002 0.0005 0.0001 0.0125 0.0036
Mitochondria 0.0073 0.0016 0.001 0.0004 0.0529 0.0001 0.0062 0.0004 0.0066 0.0007 0.0694 0.0004 0.0249 0.0004 0.0048 0.0002 0.0657 0.001
None 0.0016 0.0002 0.0018 0.0002 0.0013 0 0.0004 0.0245 0.0023 0 0.0007 0 0.0027 0 0.0001 0 0.0004 0.0001
Nuclear Periphery 0.0152 0.0114 0.0101 0.0405 0.0506 0.0119 0.0227 0.0005 0.0088 0.0006 0.017 0.0009 0.0061 0.0043 0.0022 0.0003 0.0058 0.006
Nucleolus 0.6 0.5975 0.5689 0.428 0.4856 0.651 0.4722 0.5992 0.6496 0.5207 0.3527 0.6257 0.5066 0.599 0.5998 0.5855 0.4522 0.602
Nucleus 0.3181 0.372 0.3734 0.5208 0.266 0.3302 0.403 0.3724 0.2348 0.4741 0.4431 0.3652 0.3477 0.3922 0.3618 0.41 0.4181 0.374
Peroxisomes 0.001 0.0003 0.0049 0 0.0004 0 0.0001 0 0.0137 0 0.0091 0 0.0165 0 0.0058 0 0.0029 0.0004
Punctate Nuclear 0.0078 0.0044 0.0113 0.0062 0.0673 0.0038 0.0005 0.0004 0.0045 0.0009 0.0091 0.001 0.01 0.0021 0.0008 0.0016 0.0017 0.0028
Vacuole 0.0036 0.0012 0.0015 0.0008 0.0046 0.0007 0.0029 0.0006 0.0018 0.0009 0.0026 0.0031 0.0021 0.0005 0.0023 0.0007 0.0088 0.0014
Vacuole Periphery 0.0042 0.0013 0.0033 0.0008 0.0094 0.0006 0.0064 0.0007 0.0029 0.0007 0.0035 0.0009 0.0014 0.0007 0.0008 0.0002 0.0057 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1662.0463 1436.1389 1590.4597 2025.1746 2107.6785 1383.6939 1655.15 1721.976 1514.3013 1738.6601
Translational Efficiency 1.9555 2.2038 2.0856 1.1186 1.6331 2.5065 1.8165 1.5617 1.8623 2.2504

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
507 1395 1756 1335 1613 1996 1368 1032 2120 3391 3124 2367

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 913.43 1239.83 1338.47 1232.86 895.19 1241.07 1366.06 1881.83 899.55 1240.56 1350.55 1515.81
Standard Deviation 150.15 218.38 236.33 633.27 158.14 215.00 332.32 493.88 156.46 216.40 282.74 660.38
Intensity Change Log 2 0.440776 0.551219 0.432643 0.471319 0.609755 1.071870 0.455974 0.580489 0.784199

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000330 0.000376 0.000449 0.001224 0.000286 0.000416 0.000602 0.000933 0.000296 0.000400 0.000516 0.001097
Bud Neck 0.002113 0.003855 0.006801 0.020789 0.001985 0.003684 0.005173 0.008345 0.002016 0.003754 0.006088 0.015364
Bud Site 0.000240 0.000493 0.000381 0.007975 0.000285 0.000568 0.000548 0.002186 0.000274 0.000537 0.000454 0.005451
Cell Periphery 0.000055 0.000035 0.000014 0.000640 0.000038 0.000073 0.000106 0.000061 0.000042 0.000058 0.000054 0.000387
Cytoplasm 0.000291 0.000932 0.000027 0.015107 0.000543 0.000234 0.001956 0.000501 0.000483 0.000521 0.000872 0.008739
Cytoplasmic Foci 0.000282 0.000218 0.000046 0.006530 0.000072 0.000186 0.000867 0.001915 0.000122 0.000199 0.000406 0.004518
Eisosomes 0.000030 0.000084 0.000068 0.000077 0.000042 0.000094 0.000092 0.000129 0.000039 0.000090 0.000079 0.000100
Endoplasmic Reticulum 0.002117 0.000687 0.000432 0.002304 0.001276 0.001560 0.001160 0.000271 0.001477 0.001201 0.000751 0.001418
Endosome 0.000169 0.000344 0.000025 0.006030 0.000113 0.000480 0.000232 0.001138 0.000126 0.000424 0.000116 0.003897
Golgi 0.000269 0.000142 0.000082 0.002033 0.000148 0.000229 0.000211 0.000624 0.000177 0.000193 0.000139 0.001418
Lipid Particles 0.000124 0.000168 0.000016 0.003899 0.000052 0.000086 0.000302 0.000053 0.000069 0.000120 0.000141 0.002222
Mitochondria 0.001236 0.000683 0.000876 0.006977 0.000450 0.000962 0.001234 0.002762 0.000638 0.000848 0.001032 0.005139
Mitotic Spindle 0.002003 0.002132 0.000848 0.021875 0.001207 0.001477 0.001181 0.005653 0.001397 0.001747 0.000994 0.014802
None 0.001937 0.000914 0.000354 0.016647 0.001909 0.000796 0.004372 0.001081 0.001915 0.000845 0.002113 0.009860
Nuclear Periphery 0.000124 0.000149 0.000096 0.001096 0.000135 0.000231 0.000140 0.000602 0.000133 0.000197 0.000115 0.000881
Nuclear Periphery Foci 0.000162 0.000876 0.000053 0.001884 0.002232 0.000641 0.000369 0.000339 0.001736 0.000738 0.000191 0.001210
Nucleolus 0.214286 0.380140* 0.386999* 0.205178 0.411249* 0.316072* 0.362726* 0.463318* 0.364145* 0.342429* 0.376370* 0.317726*
Nucleus 0.773034* 0.603877* 0.598473* 0.662630* 0.575932* 0.668583* 0.613362* 0.497969* 0.623069* 0.641964* 0.604993* 0.590839*
Peroxisomes 0.000916 0.002710 0.003475 0.005387 0.001687 0.001816 0.004440 0.007562 0.001502 0.002184 0.003897 0.006335
Vacuole 0.000120 0.000576 0.000158 0.008481 0.000163 0.000874 0.000426 0.002053 0.000153 0.000752 0.000275 0.005678
Vacuole Periphery 0.000164 0.000610 0.000326 0.003239 0.000196 0.000936 0.000501 0.002506 0.000188 0.000802 0.000402 0.002920

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.69 -5.00 -12.05 -11.75 -10.75 -3.12 -9.01 -6.03 -4.77 -3.16 -3.50 -8.91 -12.68 -11.31 -9.77
Bud Neck -3.56 -10.78 -14.57 -13.17 -10.98 -4.24 -9.37 -9.52 -7.10 -5.35 -5.71 -15.38 -17.17 -14.85 -12.08
Bud Site -1.41 -2.16 -9.18 -8.75 -9.02 -1.54 -1.90 -3.84 -3.40 -3.48 -1.85 -1.97 -9.77 -9.29 -9.55
Cell Periphery 0.70 1.49 -5.25 -5.64 -5.87 -1.76 -2.78 -2.15 0.51 1.74 -1.08 -0.92 -5.64 -5.31 -5.38
Cytoplasm -0.88 1.18 -7.87 -7.20 -8.04 0.79 -1.85 -0.09 -1.29 1.89 -0.16 -1.13 -7.61 -7.47 -7.01
Cytoplasmic Foci 0.25 1.02 -6.12 -6.35 -6.58 -1.30 -1.86 -2.96 -2.80 -1.61 -0.92 -1.48 -6.96 -6.82 -6.15
Eisosomes -11.21 -12.16 -9.88 1.64 -1.50 -11.96 -12.21 -10.82 -3.79 -4.10 -15.84 -16.67 -13.86 -1.55 -4.21
Endoplasmic Reticulum 9.13 10.97 -1.82 -9.65 -11.09 -1.96 1.00 11.86 10.46 8.54 2.71 8.57 -0.66 -2.76 -6.85
Endosome -1.08 1.62 -5.81 -5.53 -6.01 -2.51 -1.52 -2.06 -1.29 -1.78 -2.79 0.14 -6.18 -5.50 -6.18
Golgi 0.56 0.93 -3.27 -3.88 -4.09 -0.77 -0.77 -2.09 -1.51 -1.66 -0.30 0.54 -4.24 -4.04 -4.40
Lipid Particles -0.35 1.26 -2.80 -2.76 -2.91 -1.01 -1.17 -0.45 0.69 1.11 -0.98 -0.75 -2.85 -2.76 -2.70
Mitochondria 0.71 0.43 -3.55 -4.73 -4.55 -3.68 -11.45 -5.85 -4.32 -3.82 -1.03 -2.17 -5.77 -5.63 -5.37
Mitotic Spindle -0.13 0.70 -6.71 -7.02 -7.75 -0.26 -0.08 -3.23 -3.14 -3.33 -0.44 0.45 -7.90 -7.81 -8.39
None 0.86 1.39 -5.84 -7.24 -7.69 2.28 -2.36 1.65 -1.04 3.36 2.26 -0.36 -5.96 -7.32 -5.79
Nuclear Periphery -0.63 0.90 -6.05 -5.77 -6.23 -1.64 -0.22 -2.28 -1.79 -2.27 -1.59 0.50 -5.87 -5.31 -6.08
Nuclear Periphery Foci -1.45 1.51 -4.95 -2.23 -5.31 2.43 2.77 2.90 0.95 0.12 1.96 3.12 0.69 -2.13 -4.83
Nucleolus -13.16 -14.04 1.43 17.33 18.62 10.64 4.38 -3.89 -12.07 -7.13 2.91 -1.52 5.99 3.81 7.59
Nucleus 13.22 14.04 7.84 -5.42 -6.12 -10.33 -3.33 6.39 14.40 8.55 -2.54 2.29 3.26 5.84 1.25
Peroxisomes -10.12 -11.26 -7.64 -4.37 -2.82 -0.58 -7.00 -9.38 -8.97 -4.55 -4.28 -10.84 -11.29 -9.55 -5.20
Vacuole -2.29 -1.59 -7.35 -6.89 -7.32 -4.81 -3.44 -3.26 -2.16 -2.86 -4.92 -3.38 -7.98 -7.14 -7.82
Vacuole Periphery -3.54 -6.23 -5.71 -4.56 -5.36 -5.13 -5.39 -6.82 -4.24 -5.84 -6.18 -7.82 -8.28 -5.90 -7.55