Standard name
Description Subunit of UAF (upstream activation factor) complex; UAF is an RNA polymerase I specific transcription stimulatory factor composed of Uaf30p, Rrn5p, Rrn9p, Rrn10p, histones H3 and H4; targeting factor for the UAF that facilitates activation of many rDNA genes; deletion decreases cellular growth rate; UAF30 has a paralog, TRI1, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0.06 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.08 0.11 0.14 0.16 0.09 0.13 0.1 0.07 0.07 0 0 0.05 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0.07 0 0 0.09 0.1 0.07 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.09 0.1 0.14 0.15 0.06 0.1 0.07 0.06 0.09 0.17 0.25 0.44 0.34 0.32 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.19 0.19 0 0.05 0.05 0 0 0 0 0 0 0.2 0.21 0.21 0.14 0.17 0.12 0.16 0.09 0.11 0.11 0.13 0.14
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.22 0.22 0 0 0 0.23 0.08 0.23 0.25 0.3 0.23 0 0 0 0.12 0.1 0.11 0 0 0 0 0 0
Nucleus 0.15 0.2 0.15 0.18 0.08 0.09 0.12 0.07 0.09 0.09 0.05 0.14 0.09 0.08 0.14 0.14 0.15 0 0.08 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.49 0.29 0.16 0.13 0.08 0.11 0.07 0.16 0.16 0.12 0.16 0.4 0.34 0.28 0.21 0.25 0.32 0.27 0.38 0.33 0.22 0.13 0.11
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0
SpindlePole 0.35 0.44 0.8 0.78 0.77 0.52 0.69 0.65 0.66 0.63 0.68 0.58 0.59 0.5 0.33 0.44 0.37 0.4 0.3 0.44 0.51 0.6 0.58
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.08 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 2 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 2 0 1 3 3 7 6 9 12 22 15 0 0 1 2 0 0 3 4 0 1 2 1
Bud Neck 7 0 8 16 42 18 24 27 29 19 26 8 7 11 0 1 3 2 2 1 3 1 6
Bud Site 4 1 0 6 0 8 7 7 27 27 25 1 1 0 3 0 3
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4 7 3 14 28 16 39 12 29 19 22 16 46 54 70 39 43 0 3 0 2 3 5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 1 0 0 6 3 4 0 0 0 0 2 0
Endosome 35 35 1 8 14 0 10 3 3 5 14 37 57 45 23 20 17 36 21 15 18 29 27
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 4 0 0 2 6 1
Mitochondria 41 40 3 3 4 27 21 48 72 85 81 4 5 3 19 12 15 5 2 0 1 2 3
Nucleus 29 37 15 28 24 10 32 15 25 26 17 25 24 18 22 16 21 7 18 4 2 3 2
Nuclear Periphery 2 1 2 0 0 0 0 1 0 0 0 4 0 1 3 1 0 3 0 0 0 0 0
Nucleolus 93 54 16 20 22 13 19 33 46 35 55 72 95 61 34 29 43 60 91 45 38 30 21
Peroxisomes 4 6 0 4 0 0 1 2 2 0 8 2 10 11 1 0 2 0 1 1 0 0 0
SpindlePole 66 81 82 118 222 60 180 136 193 176 236 105 164 108 53 51 50 87 71 60 87 134 112
Vac/Vac Membrane 2 5 1 1 2 1 1 2 1 2 1 1 1 0 7 7 11 4 10 1 3 4 5
Unique Cell Count 189 184 103 152 290 116 261 210 293 280 347 181 277 217 160 115 136 222 238 138 172 226 195
Labelled Cell Count 289 267 132 223 363 160 340 295 439 417 500 276 410 313 243 179 212 222 238 138 172 226 195


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 5.0 4.9 5.0 5.6 3.7 4.7 4.1 4.0 3.8 4.0 5.2 5.4 5.4 7.1 6.8 7.0 4.9 5.1 5.1
Std Deviation (1e-4) 1.0 0.9 0.9 1.5 1.4 1.3 1.8 1.3 1.2 1.0 1.1 0.9 1.6 1.7 2.1 1.8 1.8 1.7 1.6 1.6
Intensity Change (Log2) 0.03 0.19 -0.41 -0.06 -0.27 -0.29 -0.36 -0.29 0.1 0.14 0.15 0.54 0.48 0.53 -0.0 0.06 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0.7 1.8 0 0 0 0 0 0 -1.2 0 -1.0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.0 2.2 2.9 3.2 0 2.2 1.4 1.3 1.9 3.5 4.8 7.2 5.8 5.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 4.6 4.7 4.7 3.7 4.1 3.3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 2.6 2.2 2.4
Nucleus 0.8 -1.8 -1.4 -0.6 -2.1 -1.7 -1.5 -3.4 -0.2 -1.7 -1.7 -0.2 -0.1 0.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.5 -2.3 0 0 0 0 0 0 4.2 3.6 2.5 1.2 1.8 2.9
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole -0.4 -0.6 0 0 0 0 0 0 -3.7 -3.7 -5.1 -7.4 -5.3 -6.6
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 2.5


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Uaf30-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available