| Standard name | |
|---|---|
| Description | Subunit of ISW2/yCHRAC chromatin accessibility complex; ISW2/yCHRAC also includes Itc1p, Isw2p, and Dpb4p; involved in inheritance of telomeric silencing; DLS1 has a paralog, DPB3, that arose from the whole genome duplication |
Micrographs
Sub-cellular Localization
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | 0 | – | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Bud | 0 | – | – | 0 | 0 | 1 | 1 | – | 1 | 0 | 1 | 2 | – | 0 | – | 1 | 0 | 0 | 3 | 0 | 0 | 2 | 0 | 6 |
| Bud Neck | 0 | – | – | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 2 | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
| Bud Site | 1 | – | – | 0 | 0 | 4 | 2 | – | 6 | 2 | 2 | 5 | – | 0 | – | 0 | 0 | 0 | – | – | – | – | – | – |
| Cell Periphery | 0 | – | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Cytoplasm | 5 | – | – | 15 | 20 | 9 | 6 | – | 5 | 6 | 4 | 4 | – | 1 | – | 6 | 6 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
| Endoplasmic Reticulum | 0 | – | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | – | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
| Endosome | 0 | – | – | 1 | 1 | 0 | 3 | – | 0 | 0 | 0 | 0 | – | 0 | – | 2 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 2 |
| Golgi | 0 | – | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | – | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
| Mitochondria | 2 | – | – | 2 | 1 | 66 | 13 | – | 61 | 34 | 43 | 57 | – | 1 | – | 3 | 3 | 7 | 0 | 0 | 0 | 0 | 0 | 1 |
| Nucleus | 181 | – | – | 238 | 319 | 291 | 505 | – | 264 | 294 | 206 | 274 | – | 19 | – | 249 | 255 | 177 | 177 | 22 | 40 | 272 | 400 | 423 |
| Nuclear Periphery | 0 | – | – | 0 | 2 | 2 | 9 | – | 2 | 1 | 1 | 6 | – | 0 | – | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Nucleolus | 23 | – | – | 3 | 7 | 13 | 24 | – | 36 | 34 | 35 | 42 | – | 5 | – | 20 | 18 | 16 | 11 | 0 | 4 | 22 | 42 | 59 |
| Peroxisomes | 1 | – | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| SpindlePole | 1 | – | – | 4 | 2 | 1 | 10 | – | 2 | 1 | 0 | 3 | – | 0 | – | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 2 | 5 |
| Vac/Vac Membrane | 3 | – | – | 2 | 4 | 0 | 4 | – | 0 | 2 | 1 | 3 | – | 0 | – | 6 | 6 | 3 | 5 | 0 | 0 | 3 | 13 | 39 |
| Unique Cell Count | 198 | 254 | 348 | 321 | 532 | 300 | 331 | 235 | 325 | 20 | 273 | 277 | 200 | 205 | 27 | 46 | 301 | 463 | 542 | |||||
| Labelled Cell Count | 217 | 265 | 356 | 387 | 578 | 377 | 374 | 293 | 398 | 26 | 291 | 297 | 214 | 205 | 27 | 46 | 301 | 463 | 542 | |||||
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | 8.6 | – | – | 7.7 | 8.4 | 5.1 | 6.3 | – | 5.5 | 5.9 | 5.4 | 5.7 | – | 7.5 | – | 10.7 | 11.2 | 10.9 | 7.8 | 8.1 | 8.1 |
| Std Deviation (1e-4) | 1.8 | – | – | 1.6 | 2.1 | 1.3 | 1.2 | – | 1.2 | 1.3 | 1.1 | 1.3 | – | 1.7 | – | 2.7 | 2.5 | 2.5 | 1.7 | 1.6 | 1.7 |
| Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
