Standard name
Description Subunit of ISW2/yCHRAC chromatin accessibility complex; ISW2/yCHRAC also includes Itc1p, Isw2p, and Dpb4p; involved in inheritance of telomeric silencing; DLS1 has a paralog, DPB3, that arose from the whole genome duplication

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.06 0.06 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.21 0 0.20 0.10 0.18 0.18 0.05 0 0 0 0 0 0 0 0 0
Nucleus 0.91 0.94 0.92 0.91 0.95 0.88 0.89 0.88 0.84 0.95 0.91 0.92 0.89 0.87 0.85 0.88 0.90 0.87 0.78
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.12 0 0 0 0.05 0.12 0.10 0.15 0.13 0.25 0.07 0.06 0.08 0.06 0 0.09 0.08 0.09 0.11
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 1 1 1 0 1 2 0 1 0 0 3 0 0 2 0 6
Bud Neck 0 0 0 0 1 0 0 0 2 0 0 0 0 0 0 0 0 1 0
Bud Site 1 0 0 4 2 6 2 2 5 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 15 20 9 6 5 6 4 4 1 6 6 6 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 1 1 2 0 0 0 0 0 0
Endosome 0 1 1 0 3 0 0 0 0 0 2 3 1 1 0 0 0 0 2
Golgi 0 0 0 0 0 0 0 0 0 0 1 2 0 0 0 0 0 0 2
Mitochondria 2 2 1 66 13 61 34 43 57 1 3 3 7 0 0 0 0 0 1
Nucleus 181 238 319 291 505 264 294 206 274 19 249 255 177 177 22 40 272 400 423
Nuclear Periphery 0 0 2 2 9 2 1 1 6 0 1 1 0 0 0 0 0 0 0
Nucleolus 23 3 7 13 24 36 34 35 42 5 20 18 16 11 0 4 22 42 59
Peroxisomes 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
SpindlePole 1 4 2 1 10 2 1 0 3 0 1 2 1 0 0 0 0 2 5
Vac/Vac Membrane 3 2 4 0 4 0 2 1 3 0 6 6 3 5 0 0 3 13 39
Unique Cell Count 198 254 348 321 532 300 331 235 325 20 273 277 200 205 27 46 301 463 542
Labelled Cell Count 217 265 356 387 578 377 374 293 398 26 291 297 214 205 27 46 301 463 542

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Nucleus

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.6 7.7 8.4 5.1 6.3 5.5 5.9 5.4 5.7 7.5 10.7 11.2 10.9 7.8 8.1 8.1
Std Deviation (1e-4) 1.8 1.6 2.1 1.3 1.2 1.2 1.3 1.1 1.3 1.7 2.7 2.5 2.5 1.7 1.6 1.7
Intensity Change (Log2)

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole