Standard name
Human Ortholog
Description Component of the U4/U6.U5 snRNP complex; involved in pre-mRNA splicing via spliceosome; also required for pre-5S rRNA processing and may act in concert with Rnh70p; has homology to human SART-1
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0.05 0 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 – 0 0 0 0 – 0 0 0 0 – 0.18 0.3 0.06 0.06 0.05 0 0 0 0 0.08 0
Endoplasmic Reticulum 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.06 0
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0 – 0.08 0 0.09 0.09 – 0.3 0.24 0.51 0.27 – 0.05 0.13 0 0.05 0 0 0 0 0 0 0
Nucleus 0.93 0.94 – 0.96 0.98 0.92 0.9 – 0.89 0.88 0.86 0.87 – 0.77 0.61 0.87 0.85 0.85 0.9 0.94 0.78 0.69 0.62 0.84
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0
Nucleolus 0 0 – 0 0 0 0 – 0 0 0 0 – 0.05 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0
Vac/Vac Membrane 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0.09 0.07 0.11 0 0 0 0.1 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 1 – 1 2 2 0 – 0 0 0 0 1 0 1 1 1 0 0
Bud Neck 0 0 – 0 0 0 0 – 1 1 0 0 – 0 0 1 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 2 1 – 6 3 8 10 – 0 0 1 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 1 0 – 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 4 3 – 3 5 8 14 – 5 10 2 15 – 4 7 13 13 12 1 0 0 0 2 1
Endoplasmic Reticulum 2 1 – 0 0 0 1 – 0 2 0 1 – 0 0 7 5 11 0 0 0 0 0 0
Endosome 0 0 – 0 0 0 2 – 0 1 0 1 – 0 0 3 1 3 0 0 0 1 2 0
Golgi 0 1 – 0 0 0 0 – 0 0 0 0 – 0 0 1 1 0 1 0 0 0 0 0
Mitochondria 18 0 – 7 0 23 31 – 57 82 92 98 – 1 3 4 11 8 2 0 0 0 0 0
Nucleus 150 133 – 86 198 248 324 – 170 298 154 311 – 17 14 203 194 198 148 132 28 20 23 47
Nuclear Periphery 1 0 – 0 0 1 0 – 0 0 0 0 – 0 0 1 0 0 0 0 0 0 2 0
Nucleolus 1 2 – 1 0 1 0 – 4 6 7 5 – 1 1 0 3 2 0 0 1 0 1 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 – 0 0 3 8 – 2 5 0 3 – 0 0 2 0 0 0 0 0 0 1 0
Vac/Vac Membrane 3 3 – 0 2 8 8 – 1 0 0 4 – 0 1 21 17 26 7 2 0 3 0 1
Unique Cell Count 162 141 90 203 270 362 191 338 179 359 22 23 234 228 234 165 140 36 30 38 57
Labelled Cell Count 179 143 97 205 295 390 247 410 265 448 23 26 258 245 262 165 140 36 30 38 57


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 6.6 – 5.3 6.3 5.3 5.3 – 4.4 4.6 4.1 4.7 – 5.8 4.8 8.1 7.9 7.6 – – 5.3
Std Deviation (1e-4) 0.7 1.3 – 0.8 1.1 1.3 1.3 – 0.8 1.1 0.8 1.1 – 1.1 1.1 1.6 1.5 1.6 – – 0.9
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.1193 1.5951 1.4916 1.6976 2.0471 1.8031 0.8956 1.1427 1.1229 0.8333 0.6505 1.1607 -0.1591 -0.0007 -0.1249 -0.1663 -0.4887 -0.1642
Actin 0.0067 0.0001 0.0021 0.003 0.001 0.0012 0.0059 0.0002 0.0049 0.0106 0.0025 0.002 0.0213 0.0004 0.0264 0.023 0.0027 0.0117
Bud 0.001 0.0001 0.0003 0.0004 0.0002 0.0003 0.0003 0 0.0004 0.0001 0.0001 0.0001 0.0006 0.0001 0.0002 0.0003 0.0003 0.0001
Bud Neck 0.0018 0.0001 0.0009 0.0005 0.0008 0.0014 0.0013 0.0001 0.0035 0.0018 0.0002 0.0026 0.0015 0.0001 0.0004 0.0027 0.0008 0.0008
Bud Periphery 0.0011 0.0001 0.0003 0.0004 0.0003 0.0007 0.0004 0 0.0006 0.0001 0.0002 0.0001 0.0009 0 0.0003 0.001 0.0005 0.0004
Bud Site 0.0024 0.0001 0.0015 0.0098 0.001 0.0007 0.0011 0.0001 0.0116 0.0008 0.0002 0.0028 0.0016 0.0001 0.0013 0.0013 0.0057 0.0002
Cell Periphery 0.0003 0 0.0001 0.0002 0.0002 0.0001 0.0001 0 0.0002 0.0001 0 0 0.0001 0 0.0001 0.0005 0.0005 0
Cytoplasm 0.0045 0.0005 0.0024 0.0027 0.0011 0.0008 0.0114 0.0011 0.0058 0.0035 0.0011 0.0005 0.0058 0.0105 0.0013 0.0002 0.0131 0.0006
Cytoplasmic Foci 0.0109 0 0.0044 0.0114 0.0136 0.0026 0.0298 0.0026 0.0059 0.0126 0.0041 0.0049 0.0161 0.0011 0.0057 0.0029 0.003 0.0052
Eisosomes 0.0001 0 0 0 0.0001 0 0.0001 0 0.0001 0 0 0 0.0002 0 0.0004 0.0003 0.0003 0.0001
Endoplasmic Reticulum 0.0039 0 0.0012 0.0005 0.0009 0.0002 0.0049 0.0001 0.0011 0.0015 0.0023 0.0001 0.0033 0.0001 0.0003 0.0007 0.0059 0.0005
Endosome 0.0299 0.0002 0.0128 0.0292 0.0543 0.0034 0.0373 0.0017 0.0103 0.0207 0.0395 0.0037 0.0236 0.0022 0.0037 0.0112 0.0144 0.0044
Golgi 0.0081 0 0.0055 0.008 0.036 0.0011 0.0128 0.0014 0.0049 0.0087 0.0061 0.003 0.0092 0.0023 0.0041 0.0105 0.0011 0.0074
Lipid Particles 0.0144 0.0001 0.0047 0.0165 0.077 0.0044 0.041 0.0026 0.0044 0.0128 0.0131 0.0019 0.024 0.001 0.0095 0.0114 0.0026 0.0072
Mitochondria 0.0259 0.0065 0.0086 0.0087 0.0641 0.0091 0.0113 0.0011 0.0213 0.0015 0.0045 0.0008 0.0052 0.0039 0.0077 0.0249 0.0017 0.0044
None 0.0065 0.0003 0.0005 0.0001 0.0004 0.0009 0.0053 0.0008 0.0033 0.0008 0.0005 0.0001 0.0146 0.0008 0.0086 0.0003 0.0259 0.0003
Nuclear Periphery 0.026 0.0046 0.0045 0.0005 0.0035 0.0032 0.0144 0.0089 0.0101 0.0019 0.0051 0.0008 0.0117 0.0022 0.003 0.0019 0.0171 0.0037
Nucleolus 0.0145 0.0101 0.0116 0.0067 0.0211 0.0222 0.0083 0.0055 0.0049 0.0075 0.0077 0.012 0.0078 0.0062 0.0043 0.0042 0.0097 0.0115
Nucleus 0.8077 0.9673 0.9228 0.8378 0.7087 0.9192 0.7727 0.9699 0.8937 0.9077 0.8655 0.9611 0.8155 0.9601 0.9099 0.8922 0.8812 0.9167
Peroxisomes 0.0046 0 0.0015 0.0287 0.0077 0.005 0.0213 0.0023 0.0057 0.0043 0.0443 0.0018 0.0091 0.0007 0.0101 0.0041 0.0005 0.0231
Punctate Nuclear 0.0211 0.0096 0.0103 0.0308 0.0038 0.0228 0.015 0.0013 0.0034 0.0021 0.0008 0.0014 0.0246 0.0077 0.0023 0.0007 0.0081 0.001
Vacuole 0.0053 0.0001 0.0032 0.0038 0.0025 0.0003 0.0031 0.0001 0.0027 0.0004 0.0009 0.0002 0.0023 0.0002 0.0004 0.0037 0.004 0.0002
Vacuole Periphery 0.0032 0.0002 0.0009 0.0005 0.0018 0.0003 0.0021 0.0001 0.0011 0.0003 0.0011 0.0001 0.0011 0.0002 0.0003 0.0018 0.0009 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.0082 9.5059 14.5225 10.0311 10.5431 7.502 8.7633 10.8753 10.1586 11.5729
Translational Efficiency 2.0456 1.5834 1.1359 1.8348 1.8321 2.5523 1.6812 1.311 1.501 1.399

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1605 1020 506 1303 1560 1335 1645 2745 3165 2355 2151 4048

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 620.54 699.04 1006.80 872.10 726.96 758.76 921.70 929.09 672.99 732.89 941.72 910.75
Standard Deviation 66.80 130.01 122.85 134.24 89.76 101.19 107.96 128.35 95.21 118.33 117.33 132.97
Intensity Change Log 2 — 0.171851 0.698181 0.490969 — 0.061768 0.342421 0.353942 — 0.113508 0.517194 0.418665

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000042 0.000621 0.001126 0.001075 0.000185 0.000305 0.000401 0.000627 0.000112 0.000442 0.000571 0.000771
Bud Neck 0.002598 0.005676 0.008654 0.023133 0.001222 0.006915 0.007107 0.031241 0.001920 0.006379 0.007471 0.028631
Bud Site 0.002128 0.003021 0.007535 0.013201 0.000435 0.003981 0.000855 0.012623 0.001294 0.003565 0.002427 0.012809
Cell Periphery 0.000162 0.000336 0.000100 0.000223 0.000084 0.000144 0.000083 0.000148 0.000123 0.000227 0.000087 0.000173
Cytoplasm 0.067636 0.039889 0.024195 0.034806 0.017802 0.017759 0.013798 0.050128 0.043073 0.027344 0.016244 0.045196
Cytoplasmic Foci 0.008555 0.008330 0.000435 0.001825 0.001800 0.004447 0.000310 0.000875 0.005226 0.006129 0.000340 0.001181
Eisosomes 0.000019 0.000039 0.000024 0.000023 0.000014 0.000022 0.000020 0.000019 0.000017 0.000029 0.000021 0.000021
Endoplasmic Reticulum 0.001072 0.006550 0.008802 0.008515 0.002440 0.005187 0.005281 0.003744 0.001746 0.005777 0.006110 0.005280
Endosome 0.000677 0.001579 0.000784 0.002678 0.000288 0.001267 0.000538 0.001907 0.000485 0.001402 0.000596 0.002155
Golgi 0.000086 0.000711 0.000103 0.001701 0.000057 0.001666 0.000062 0.000470 0.000072 0.001252 0.000071 0.000866
Lipid Particles 0.001016 0.001760 0.000475 0.000339 0.000717 0.001433 0.000229 0.000205 0.000868 0.001575 0.000287 0.000249
Mitochondria 0.000830 0.004796 0.001115 0.004864 0.001381 0.011568 0.001014 0.006068 0.001102 0.008635 0.001038 0.005680
Mitotic Spindle 0.001280 0.002068 0.000596 0.021865 0.001137 0.007792 0.001604 0.027947 0.001209 0.005313 0.001367 0.025990
None 0.016371 0.024387 0.002450 0.007161 0.006576 0.005147 0.002920 0.011340 0.011544 0.013481 0.002809 0.009995
Nuclear Periphery 0.001251 0.001592 0.001256 0.001882 0.000773 0.003563 0.001136 0.002561 0.001016 0.002709 0.001164 0.002342
Nuclear Periphery Foci 0.000208 0.001042 0.000579 0.000576 0.000219 0.001173 0.000258 0.000250 0.000214 0.001116 0.000334 0.000355
Nucleolus 0.003799 0.013090 0.001084 0.001935 0.005594 0.006679 0.002291 0.002726 0.004684 0.009456 0.002007 0.002471
Nucleus 0.890373* 0.877405* 0.928960* 0.864581* 0.957184* 0.911945* 0.958273* 0.835546* 0.923304* 0.896985* 0.951378* 0.844892*
Peroxisomes 0.000540 0.001843 0.000783 0.000341 0.000288 0.002413 0.000087 0.000762 0.000416 0.002166 0.000251 0.000627
Vacuole 0.001200 0.003979 0.010786 0.008599 0.001488 0.005363 0.003634 0.009556 0.001342 0.004763 0.005316 0.009248
Vacuole Periphery 0.000156 0.001287 0.000160 0.000675 0.000315 0.001232 0.000099 0.001257 0.000235 0.001256 0.000113 0.001070

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.88 -6.40 -7.70 -2.60 0.80 -1.54 -4.33 -3.74 -3.14 -0.82 -5.01 -9.52 -8.16 -3.40 -0.60
Bud Neck -4.22 -6.12 -12.26 -10.23 -6.91 -6.46 -13.23 -20.73 -13.61 -13.30 -7.44 -13.18 -23.57 -17.45 -15.03
Bud Site -1.03 -2.20 -4.76 -4.05 -0.72 -3.63 -5.22 -8.61 -3.31 -7.72 -3.21 -2.18 -8.83 -5.16 -6.29
Cell Periphery -2.81 2.88 -0.92 2.40 -3.15 -1.74 0.07 -1.74 1.13 -2.39 -3.11 3.48 -0.61 2.90 -4.25
Cytoplasm 5.52 9.55 8.69 2.53 -1.66 0.10 1.78 -9.14 -9.81 -12.14 5.76 10.96 2.08 -4.61 -11.36
Cytoplasmic Foci -0.12 7.24 6.44 4.88 -1.66 -2.73 3.17 2.83 5.30 -1.42 -1.02 7.94 7.31 7.06 -2.26
Eisosomes -5.12 -3.92 -5.78 1.98 -0.41 -4.58 -8.13 -8.92 -0.88 1.10 -6.41 -7.08 -8.41 1.77 0.06
Endoplasmic Reticulum -11.75 -13.49 -16.05 -4.62 0.78 -4.64 -10.11 -5.81 1.11 6.69 -10.56 -18.89 -16.65 -1.24 3.81
Endosome -2.25 -1.15 -2.13 -0.14 -1.63 -5.10 -3.67 -3.48 0.66 -1.85 -4.31 -1.72 -3.39 0.52 -2.60
Golgi -2.45 -1.29 -1.46 -0.23 -1.40 -2.35 -0.59 -3.13 2.08 -2.94 -2.94 -0.47 -2.08 1.86 -2.04
Lipid Particles -3.10 2.71 4.66 6.34 2.22 -3.36 3.54 4.02 6.51 1.40 -4.44 5.55 6.59 9.23 2.09
Mitochondria -3.00 -2.54 -4.21 0.74 -3.61 -5.13 0.36 -4.15 3.64 -6.10 -5.99 -0.35 -6.32 3.48 -7.07
Mitotic Spindle -0.67 0.33 -5.61 -5.38 -5.84 -3.69 -1.48 -10.40 -5.62 -9.49 -3.64 -1.27 -11.76 -8.14 -10.94
None -2.48 8.26 6.18 6.88 -5.76 1.52 5.71 -1.69 -2.99 -7.32 -1.14 9.66 3.93 4.35 -8.75
Nuclear Periphery -0.89 -1.22 -4.30 -5.14 -6.42 -5.53 -7.76 -13.39 0.56 -8.29 -4.80 -3.08 -9.25 -1.45 -10.22
Nuclear Periphery Foci -4.33 -4.69 -4.02 0.98 0.26 -1.46 -2.46 -0.50 1.42 2.39 -2.44 -4.54 -3.75 1.83 1.23
Nucleolus -6.71 6.46 2.77 7.64 -2.57 -1.14 5.90 4.56 4.12 -1.74 -5.77 7.31 5.05 8.34 -2.36
Nucleus 1.60 -3.02 2.89 0.99 5.56 7.17 2.15 19.49 8.08 19.03 5.07 -4.30 13.96 6.81 19.43
Peroxisomes -3.27 -0.10 2.43 4.23 0.84 -2.95 3.72 -0.37 2.75 -1.40 -3.95 1.78 0.70 4.05 -0.67
Vacuole -5.21 -7.48 -8.61 -6.86 0.36 -4.93 -5.97 -11.94 -7.87 -6.54 -6.95 -9.67 -15.04 -10.73 -5.34
Vacuole Periphery -1.60 -0.46 -2.72 1.22 -2.64 -2.74 1.13 -1.20 2.00 -3.27 -2.88 1.19 -2.17 1.99 -3.92
Standard name
Human Ortholog
Description Component of the U4/U6.U5 snRNP complex; involved in pre-mRNA splicing via spliceosome; also required for pre-5S rRNA processing and may act in concert with Rnh70p; has homology to human SART-1
Localization
Cell Percentages nucleus (97%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Snu66

Snu66


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Snu66-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available