Standard name
Human Ortholog
Description Component of the U4/U6.U5 snRNP complex; involved in pre-mRNA splicing via spliceosome; also required for pre-5S rRNA processing and may act in concert with Rnh70p; has homology to human SART-1

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0.18 0.3 0.06 0.06 0.05 0 0 0 0 0.08 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0 0.08 0 0.09 0.09 0.3 0.24 0.51 0.27 0.05 0.13 0 0.05 0 0 0 0 0 0 0
Nucleus 0.93 0.94 0.96 0.98 0.92 0.9 0.89 0.88 0.86 0.87 0.77 0.61 0.87 0.85 0.85 0.9 0.94 0.78 0.69 0.62 0.84
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.07 0.11 0 0 0 0.1 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 1 2 2 0 0 0 0 0 1 0 1 1 1 0 0
Bud Neck 0 0 0 0 0 0 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 2 1 6 3 8 10 0 0 1 0 0
Cell Periphery 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 4 3 3 5 8 14 5 10 2 15 4 7 13 13 12 1 0 0 0 2 1
Endoplasmic Reticulum 2 1 0 0 0 1 0 2 0 1 0 0 7 5 11 0 0 0 0 0 0
Endosome 0 0 0 0 0 2 0 1 0 1 0 0 3 1 3 0 0 0 1 2 0
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 1 1 0 1 0 0 0 0 0
Mitochondria 18 0 7 0 23 31 57 82 92 98 1 3 4 11 8 2 0 0 0 0 0
Nucleus 150 133 86 198 248 324 170 298 154 311 17 14 203 194 198 148 132 28 20 23 47
Nuclear Periphery 1 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 2 0
Nucleolus 1 2 1 0 1 0 4 6 7 5 1 1 0 3 2 0 0 1 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 3 8 2 5 0 3 0 0 2 0 0 0 0 0 0 1 0
Vac/Vac Membrane 3 3 0 2 8 8 1 0 0 4 0 1 21 17 26 7 2 0 3 0 1
Unique Cell Count 162 141 90 203 270 362 191 338 179 359 22 23 234 228 234 165 140 36 30 38 57
Labelled Cell Count 179 143 97 205 295 390 247 410 265 448 23 26 258 245 262 165 140 36 30 38 57


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 6.6 5.3 6.3 5.3 5.3 4.4 4.6 4.1 4.7 5.8 4.8 8.1 7.9 7.6 5.3
Std Deviation (1e-4) 0.7 1.3 0.8 1.1 1.3 1.3 0.8 1.1 0.8 1.1 1.1 1.1 1.6 1.5 1.6 0.9
Intensity Change (Log2)


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.1193 1.5951 1.4916 1.6976 2.0471 1.8031 0.8956 1.1427 1.1229 0.8333 0.6505 1.1607 -0.1591 -0.0007 -0.1249 -0.1663 -0.4887 -0.1642
Actin 0.0067 0.0001 0.0021 0.003 0.001 0.0012 0.0059 0.0002 0.0049 0.0106 0.0025 0.002 0.0213 0.0004 0.0264 0.023 0.0027 0.0117
Bud 0.001 0.0001 0.0003 0.0004 0.0002 0.0003 0.0003 0 0.0004 0.0001 0.0001 0.0001 0.0006 0.0001 0.0002 0.0003 0.0003 0.0001
Bud Neck 0.0018 0.0001 0.0009 0.0005 0.0008 0.0014 0.0013 0.0001 0.0035 0.0018 0.0002 0.0026 0.0015 0.0001 0.0004 0.0027 0.0008 0.0008
Bud Periphery 0.0011 0.0001 0.0003 0.0004 0.0003 0.0007 0.0004 0 0.0006 0.0001 0.0002 0.0001 0.0009 0 0.0003 0.001 0.0005 0.0004
Bud Site 0.0024 0.0001 0.0015 0.0098 0.001 0.0007 0.0011 0.0001 0.0116 0.0008 0.0002 0.0028 0.0016 0.0001 0.0013 0.0013 0.0057 0.0002
Cell Periphery 0.0003 0 0.0001 0.0002 0.0002 0.0001 0.0001 0 0.0002 0.0001 0 0 0.0001 0 0.0001 0.0005 0.0005 0
Cytoplasm 0.0045 0.0005 0.0024 0.0027 0.0011 0.0008 0.0114 0.0011 0.0058 0.0035 0.0011 0.0005 0.0058 0.0105 0.0013 0.0002 0.0131 0.0006
Cytoplasmic Foci 0.0109 0 0.0044 0.0114 0.0136 0.0026 0.0298 0.0026 0.0059 0.0126 0.0041 0.0049 0.0161 0.0011 0.0057 0.0029 0.003 0.0052
Eisosomes 0.0001 0 0 0 0.0001 0 0.0001 0 0.0001 0 0 0 0.0002 0 0.0004 0.0003 0.0003 0.0001
Endoplasmic Reticulum 0.0039 0 0.0012 0.0005 0.0009 0.0002 0.0049 0.0001 0.0011 0.0015 0.0023 0.0001 0.0033 0.0001 0.0003 0.0007 0.0059 0.0005
Endosome 0.0299 0.0002 0.0128 0.0292 0.0543 0.0034 0.0373 0.0017 0.0103 0.0207 0.0395 0.0037 0.0236 0.0022 0.0037 0.0112 0.0144 0.0044
Golgi 0.0081 0 0.0055 0.008 0.036 0.0011 0.0128 0.0014 0.0049 0.0087 0.0061 0.003 0.0092 0.0023 0.0041 0.0105 0.0011 0.0074
Lipid Particles 0.0144 0.0001 0.0047 0.0165 0.077 0.0044 0.041 0.0026 0.0044 0.0128 0.0131 0.0019 0.024 0.001 0.0095 0.0114 0.0026 0.0072
Mitochondria 0.0259 0.0065 0.0086 0.0087 0.0641 0.0091 0.0113 0.0011 0.0213 0.0015 0.0045 0.0008 0.0052 0.0039 0.0077 0.0249 0.0017 0.0044
None 0.0065 0.0003 0.0005 0.0001 0.0004 0.0009 0.0053 0.0008 0.0033 0.0008 0.0005 0.0001 0.0146 0.0008 0.0086 0.0003 0.0259 0.0003
Nuclear Periphery 0.026 0.0046 0.0045 0.0005 0.0035 0.0032 0.0144 0.0089 0.0101 0.0019 0.0051 0.0008 0.0117 0.0022 0.003 0.0019 0.0171 0.0037
Nucleolus 0.0145 0.0101 0.0116 0.0067 0.0211 0.0222 0.0083 0.0055 0.0049 0.0075 0.0077 0.012 0.0078 0.0062 0.0043 0.0042 0.0097 0.0115
Nucleus 0.8077 0.9673 0.9228 0.8378 0.7087 0.9192 0.7727 0.9699 0.8937 0.9077 0.8655 0.9611 0.8155 0.9601 0.9099 0.8922 0.8812 0.9167
Peroxisomes 0.0046 0 0.0015 0.0287 0.0077 0.005 0.0213 0.0023 0.0057 0.0043 0.0443 0.0018 0.0091 0.0007 0.0101 0.0041 0.0005 0.0231
Punctate Nuclear 0.0211 0.0096 0.0103 0.0308 0.0038 0.0228 0.015 0.0013 0.0034 0.0021 0.0008 0.0014 0.0246 0.0077 0.0023 0.0007 0.0081 0.001
Vacuole 0.0053 0.0001 0.0032 0.0038 0.0025 0.0003 0.0031 0.0001 0.0027 0.0004 0.0009 0.0002 0.0023 0.0002 0.0004 0.0037 0.004 0.0002
Vacuole Periphery 0.0032 0.0002 0.0009 0.0005 0.0018 0.0003 0.0021 0.0001 0.0011 0.0003 0.0011 0.0001 0.0011 0.0002 0.0003 0.0018 0.0009 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.0082 9.5059 14.5225 10.0311 10.5431 7.502 8.7633 10.8753 10.1586 11.5729
Translational Efficiency 2.0456 1.5834 1.1359 1.8348 1.8321 2.5523 1.6812 1.311 1.501 1.399

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Component of the U4/U6.U5 snRNP complex; involved in pre-mRNA splicing via spliceosome; also required for pre-5S rRNA processing and may act in concert with Rnh70p; has homology to human SART-1
Localization
Cell Percentages nucleus (97%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Snu66

Snu66


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Snu66-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available