Standard name
Description Component of the U4/U6.U5 snRNP complex; involved in pre-mRNA splicing via spliceosome; also required for pre-5S rRNA processing and may act in concert with Rnh70p; has homology to human SART-1

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0.18 0.3 0.06 0.06 0.05 0 0 0 0 0.08 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0 0.08 0 0.09 0.09 0.3 0.24 0.51 0.27 0.05 0.13 0 0.05 0 0 0 0 0 0 0
Nucleus 0.93 0.94 0.96 0.98 0.92 0.9 0.89 0.88 0.86 0.87 0.77 0.61 0.87 0.85 0.85 0.9 0.94 0.78 0.69 0.62 0.84
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.07 0.11 0 0 0 0.1 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 1 2 2 0 0 0 0 0 1 0 1 1 1 0 0
Bud Neck 0 0 0 0 0 0 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 2 1 6 3 8 10 0 0 1 0 0
Cell Periphery 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 4 3 3 5 8 14 5 10 2 15 4 7 13 13 12 1 0 0 0 2 1
Endoplasmic Reticulum 2 1 0 0 0 1 0 2 0 1 0 0 7 5 11 0 0 0 0 0 0
Endosome 0 0 0 0 0 2 0 1 0 1 0 0 3 1 3 0 0 0 1 2 0
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 1 1 0 1 0 0 0 0 0
Mitochondria 18 0 7 0 23 31 57 82 92 98 1 3 4 11 8 2 0 0 0 0 0
Nucleus 150 133 86 198 248 324 170 298 154 311 17 14 203 194 198 148 132 28 20 23 47
Nuclear Periphery 1 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 2 0
Nucleolus 1 2 1 0 1 0 4 6 7 5 1 1 0 3 2 0 0 1 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 3 8 2 5 0 3 0 0 2 0 0 0 0 0 0 1 0
Vac/Vac Membrane 3 3 0 2 8 8 1 0 0 4 0 1 21 17 26 7 2 0 3 0 1
Unique Cell Count 162 141 90 203 270 362 191 338 179 359 22 23 234 228 234 165 140 36 30 38 57
Labelled Cell Count 179 143 97 205 295 390 247 410 265 448 23 26 258 245 262 165 140 36 30 38 57


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 6.6 5.3 6.3 5.3 5.3 4.4 4.6 4.1 4.7 5.8 4.8 8.1 7.9 7.6 5.3
Std Deviation (1e-4) 0.7 1.3 0.8 1.1 1.3 1.3 0.8 1.1 0.8 1.1 1.1 1.1 1.6 1.5 1.6 0.9
Intensity Change (Log2)


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Snu66-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available