Standard name | |
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Human Ortholog | |
Description | Component of the U4/U6.U5 snRNP complex; involved in pre-mRNA splicing via spliceosome; also required for pre-5S rRNA processing and may act in concert with Rnh70p; has homology to human SART-1 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | – | 0 | 0 | 0 | 1 | – | 1 | 2 | 2 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 0 |
Bud Neck | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 1 | 1 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | – | 0 | 0 | 2 | 1 | – | 6 | 3 | 8 | 10 | – | 0 | 0 | 1 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | – | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 3 | – | 3 | 5 | 8 | 14 | – | 5 | 10 | 2 | 15 | – | 4 | 7 | 13 | 13 | 12 | 1 | 0 | 0 | 0 | 2 | 1 |
Endoplasmic Reticulum | 2 | 1 | – | 0 | 0 | 0 | 1 | – | 0 | 2 | 0 | 1 | – | 0 | 0 | 7 | 5 | 11 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | – | 0 | 0 | 0 | 2 | – | 0 | 1 | 0 | 1 | – | 0 | 0 | 3 | 1 | 3 | 0 | 0 | 0 | 1 | 2 | 0 |
Golgi | 0 | 1 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 18 | 0 | – | 7 | 0 | 23 | 31 | – | 57 | 82 | 92 | 98 | – | 1 | 3 | 4 | 11 | 8 | 2 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 150 | 133 | – | 86 | 198 | 248 | 324 | – | 170 | 298 | 154 | 311 | – | 17 | 14 | 203 | 194 | 198 | 148 | 132 | 28 | 20 | 23 | 47 |
Nuclear Periphery | 1 | 0 | – | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 |
Nucleolus | 1 | 2 | – | 1 | 0 | 1 | 0 | – | 4 | 6 | 7 | 5 | – | 1 | 1 | 0 | 3 | 2 | 0 | 0 | 1 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | – | 0 | 0 | 3 | 8 | – | 2 | 5 | 0 | 3 | – | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Vac/Vac Membrane | 3 | 3 | – | 0 | 2 | 8 | 8 | – | 1 | 0 | 0 | 4 | – | 0 | 1 | 21 | 17 | 26 | 7 | 2 | 0 | 3 | 0 | 1 |
Unique Cell Count | 162 | 141 | 90 | 203 | 270 | 362 | 191 | 338 | 179 | 359 | 22 | 23 | 234 | 228 | 234 | 165 | 140 | 36 | 30 | 38 | 57 | |||
Labelled Cell Count | 179 | 143 | 97 | 205 | 295 | 390 | 247 | 410 | 265 | 448 | 23 | 26 | 258 | 245 | 262 | 165 | 140 | 36 | 30 | 38 | 57 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.8 | 6.6 | – | 5.3 | 6.3 | 5.3 | 5.3 | – | 4.4 | 4.6 | 4.1 | 4.7 | – | 5.8 | 4.8 | 8.1 | 7.9 | 7.6 | – | – | 5.3 |
Std Deviation (1e-4) | 0.7 | 1.3 | – | 0.8 | 1.1 | 1.3 | 1.3 | – | 0.8 | 1.1 | 0.8 | 1.1 | – | 1.1 | 1.1 | 1.6 | 1.5 | 1.6 | – | – | 0.9 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.1193 | 1.5951 | 1.4916 | 1.6976 | 2.0471 | 1.8031 | 0.8956 | 1.1427 | 1.1229 | 0.8333 | 0.6505 | 1.1607 | -0.1591 | -0.0007 | -0.1249 | -0.1663 | -0.4887 | -0.1642 |
Actin | 0.0067 | 0.0001 | 0.0021 | 0.003 | 0.001 | 0.0012 | 0.0059 | 0.0002 | 0.0049 | 0.0106 | 0.0025 | 0.002 | 0.0213 | 0.0004 | 0.0264 | 0.023 | 0.0027 | 0.0117 |
Bud | 0.001 | 0.0001 | 0.0003 | 0.0004 | 0.0002 | 0.0003 | 0.0003 | 0 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0001 | 0.0002 | 0.0003 | 0.0003 | 0.0001 |
Bud Neck | 0.0018 | 0.0001 | 0.0009 | 0.0005 | 0.0008 | 0.0014 | 0.0013 | 0.0001 | 0.0035 | 0.0018 | 0.0002 | 0.0026 | 0.0015 | 0.0001 | 0.0004 | 0.0027 | 0.0008 | 0.0008 |
Bud Periphery | 0.0011 | 0.0001 | 0.0003 | 0.0004 | 0.0003 | 0.0007 | 0.0004 | 0 | 0.0006 | 0.0001 | 0.0002 | 0.0001 | 0.0009 | 0 | 0.0003 | 0.001 | 0.0005 | 0.0004 |
Bud Site | 0.0024 | 0.0001 | 0.0015 | 0.0098 | 0.001 | 0.0007 | 0.0011 | 0.0001 | 0.0116 | 0.0008 | 0.0002 | 0.0028 | 0.0016 | 0.0001 | 0.0013 | 0.0013 | 0.0057 | 0.0002 |
Cell Periphery | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0005 | 0.0005 | 0 |
Cytoplasm | 0.0045 | 0.0005 | 0.0024 | 0.0027 | 0.0011 | 0.0008 | 0.0114 | 0.0011 | 0.0058 | 0.0035 | 0.0011 | 0.0005 | 0.0058 | 0.0105 | 0.0013 | 0.0002 | 0.0131 | 0.0006 |
Cytoplasmic Foci | 0.0109 | 0 | 0.0044 | 0.0114 | 0.0136 | 0.0026 | 0.0298 | 0.0026 | 0.0059 | 0.0126 | 0.0041 | 0.0049 | 0.0161 | 0.0011 | 0.0057 | 0.0029 | 0.003 | 0.0052 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 | 0.0004 | 0.0003 | 0.0003 | 0.0001 |
Endoplasmic Reticulum | 0.0039 | 0 | 0.0012 | 0.0005 | 0.0009 | 0.0002 | 0.0049 | 0.0001 | 0.0011 | 0.0015 | 0.0023 | 0.0001 | 0.0033 | 0.0001 | 0.0003 | 0.0007 | 0.0059 | 0.0005 |
Endosome | 0.0299 | 0.0002 | 0.0128 | 0.0292 | 0.0543 | 0.0034 | 0.0373 | 0.0017 | 0.0103 | 0.0207 | 0.0395 | 0.0037 | 0.0236 | 0.0022 | 0.0037 | 0.0112 | 0.0144 | 0.0044 |
Golgi | 0.0081 | 0 | 0.0055 | 0.008 | 0.036 | 0.0011 | 0.0128 | 0.0014 | 0.0049 | 0.0087 | 0.0061 | 0.003 | 0.0092 | 0.0023 | 0.0041 | 0.0105 | 0.0011 | 0.0074 |
Lipid Particles | 0.0144 | 0.0001 | 0.0047 | 0.0165 | 0.077 | 0.0044 | 0.041 | 0.0026 | 0.0044 | 0.0128 | 0.0131 | 0.0019 | 0.024 | 0.001 | 0.0095 | 0.0114 | 0.0026 | 0.0072 |
Mitochondria | 0.0259 | 0.0065 | 0.0086 | 0.0087 | 0.0641 | 0.0091 | 0.0113 | 0.0011 | 0.0213 | 0.0015 | 0.0045 | 0.0008 | 0.0052 | 0.0039 | 0.0077 | 0.0249 | 0.0017 | 0.0044 |
None | 0.0065 | 0.0003 | 0.0005 | 0.0001 | 0.0004 | 0.0009 | 0.0053 | 0.0008 | 0.0033 | 0.0008 | 0.0005 | 0.0001 | 0.0146 | 0.0008 | 0.0086 | 0.0003 | 0.0259 | 0.0003 |
Nuclear Periphery | 0.026 | 0.0046 | 0.0045 | 0.0005 | 0.0035 | 0.0032 | 0.0144 | 0.0089 | 0.0101 | 0.0019 | 0.0051 | 0.0008 | 0.0117 | 0.0022 | 0.003 | 0.0019 | 0.0171 | 0.0037 |
Nucleolus | 0.0145 | 0.0101 | 0.0116 | 0.0067 | 0.0211 | 0.0222 | 0.0083 | 0.0055 | 0.0049 | 0.0075 | 0.0077 | 0.012 | 0.0078 | 0.0062 | 0.0043 | 0.0042 | 0.0097 | 0.0115 |
Nucleus | 0.8077 | 0.9673 | 0.9228 | 0.8378 | 0.7087 | 0.9192 | 0.7727 | 0.9699 | 0.8937 | 0.9077 | 0.8655 | 0.9611 | 0.8155 | 0.9601 | 0.9099 | 0.8922 | 0.8812 | 0.9167 |
Peroxisomes | 0.0046 | 0 | 0.0015 | 0.0287 | 0.0077 | 0.005 | 0.0213 | 0.0023 | 0.0057 | 0.0043 | 0.0443 | 0.0018 | 0.0091 | 0.0007 | 0.0101 | 0.0041 | 0.0005 | 0.0231 |
Punctate Nuclear | 0.0211 | 0.0096 | 0.0103 | 0.0308 | 0.0038 | 0.0228 | 0.015 | 0.0013 | 0.0034 | 0.0021 | 0.0008 | 0.0014 | 0.0246 | 0.0077 | 0.0023 | 0.0007 | 0.0081 | 0.001 |
Vacuole | 0.0053 | 0.0001 | 0.0032 | 0.0038 | 0.0025 | 0.0003 | 0.0031 | 0.0001 | 0.0027 | 0.0004 | 0.0009 | 0.0002 | 0.0023 | 0.0002 | 0.0004 | 0.0037 | 0.004 | 0.0002 |
Vacuole Periphery | 0.0032 | 0.0002 | 0.0009 | 0.0005 | 0.0018 | 0.0003 | 0.0021 | 0.0001 | 0.0011 | 0.0003 | 0.0011 | 0.0001 | 0.0011 | 0.0002 | 0.0003 | 0.0018 | 0.0009 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.0082 | 9.5059 | 14.5225 | 10.0311 | 10.5431 | 7.502 | 8.7633 | 10.8753 | 10.1586 | 11.5729 |
Translational Efficiency | 2.0456 | 1.5834 | 1.1359 | 1.8348 | 1.8321 | 2.5523 | 1.6812 | 1.311 | 1.501 | 1.399 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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