Standard name
Human Ortholog
Description Nuclear protein putative transcription factor; required for growth of superficial pseudohyphae (which do not invade the agar substrate) but not for invasive pseudohyphal growth; may act together with Phd1p; potential Cdc28p substrate
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.91 0.96 1.0 0.97 0.97 0.95 0.88 0.87 0.72 0.82 0.69 0.63 0.99 1.0 1.0 0.86 0.86 0.87 0.8 0.86 0.82 0.92 0.88 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0.06 0 0.06 0 0.08 0.19 0.2 0.45 0.27 0.56 0.5 0 0 0 0 0 0 0.05 0.06 0.1 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.15 0.09 0.09 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 1 1 2
Bud 0 0 0 0 0 1 1 5 4 5 0 6 0 0 0 0 0 1 1 0 0 0 4 3
Bud Neck 1 0 0 1 4 3 3 4 0 1 0 0 3 1 0 0 0 0 2 0 0 0 0 1
Bud Site 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 1 0 2 2 0 0 0 2 1 5 1 6 0 0 0 0 1 1
Cytoplasm 255 111 42 91 238 167 218 245 166 201 70 85 218 218 216 262 119 136 229 107 59 123 215 237
Endoplasmic Reticulum 4 0 0 0 0 0 0 0 0 0 0 1 2 0 0 7 6 5 0 0 0 0 1 2
Endosome 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 4 0 1 2 0 0 0 1 2
Golgi 1 0 0 0 1 0 0 0 0 1 0 0 0 1 1 1 1 0 1 0 0 0 1 0
Mitochondria 17 7 0 6 4 14 47 57 102 66 57 68 0 0 0 3 2 6 14 7 7 3 4 8
Nucleus 3 0 0 0 0 1 7 12 4 8 1 3 0 0 0 5 5 7 7 2 0 0 0 1
Nuclear Periphery 0 0 0 0 0 1 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 0 0 0 0 2 1 1 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 0 0 0 0 2 0 0 4 3 1 0 0 0 0 0 0 0 0 5 0 0 0 1 1
Vac/Vac Membrane 10 1 0 1 3 1 5 0 7 4 1 3 0 0 0 45 13 14 10 3 0 0 1 2
Unique Cell Count 279 116 42 94 245 175 248 282 229 246 102 135 220 219 217 303 139 157 289 124 73 134 245 270
Labelled Cell Count 291 120 42 100 252 188 283 329 289 294 130 167 223 222 218 332 147 177 289 124 73 134 245 270


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.4 3.7 4.4 4.1 4.7 4.1 3.9 4.2 3.4 4.0 3.5 3.7 4.7 4.8 4.9 6.3 6.6 6.5 4.1 4.8 4.9
Std Deviation (1e-4) 0.6 0.5 0.8 1.5 1.9 2.5 2.0 1.4 1.3 1.7 1.6 1.0 0.8 1.1 1.3 1.2 1.3 1.4 0.9 1.7 1.6
Intensity Change (Log2) – – – -0.09 0.11 -0.08 -0.16 -0.08 -0.35 -0.13 -0.31 -0.26 0.11 0.15 0.16 0.53 0.59 0.57 -0.09 0.14 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 – –
Bud Neck – – – – – – – – – – – – 0 – –
Bud Site – – – – – – – – – – – – 0 – –
Cell Periphery – – – – – – – – – – – – 0 – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 – –
Endosome – – – – – – – – – – – – 0 – –
Golgi – – – – – – – – – – – – 0 – –
Mitochondria – – – – – – – – – – – – 0 – –
Nuclear Periphery – – – – – – – – – – – – 0 – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 – –
Peroxisomes – – – – – – – – – – – – 0 – –
SpindlePole – – – – – – – – – – – – 0 – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 – –
Cytoplasm – – – – – – – – – – – – 0 – –
Nucleus – – – – – – – – – – – – 0 – –
Vacuole – – – – – – – – – – – – 2.7 – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.7993 1.4865 1.1804 1.0469 1.3557 1.2872 -0.4487 0.4047 -0.0104 0.6969 0.3341 0.2897 -1.1063 -0.7274 -1.0622 -0.1894 -1.0485 -0.6111
Actin 0.0161 0.0001 0.0108 0.0146 0.0415 0.0024 0.0123 0.0005 0.0004 0.0019 0.0086 0.0044 0.0216 0.0006 0.0062 0.0387 0.002 0.0119
Bud 0.0007 0.0003 0.0032 0.0009 0.0007 0.0019 0.0005 0.0024 0.0003 0.0068 0.0004 0.0004 0.0005 0.0001 0.0002 0.0012 0 0.0003
Bud Neck 0.0044 0.0004 0.0021 0.0058 0.001 0.001 0.0025 0.001 0.0004 0.003 0.0016 0.0031 0.0108 0.0002 0.0002 0.0009 0.0002 0.0012
Bud Periphery 0.0012 0.0002 0.0041 0.0012 0.001 0.0056 0.0005 0.0017 0.0002 0.0048 0.0008 0.0005 0.0008 0.0001 0.0004 0.0012 0 0.0003
Bud Site 0.0035 0.0129 0.0066 0.0086 0.0011 0.0004 0.0021 0.0242 0.001 0.0157 0.0024 0.0004 0.0063 0.0013 0.0008 0.007 0.0001 0.0011
Cell Periphery 0.0003 0.0001 0.0002 0.0002 0.0001 0.0001 0.0002 0.0003 0.0001 0.0005 0.0002 0 0.0005 0.0002 0.0001 0.0002 0 0.0001
Cytoplasm 0.2348 0.2848 0.2292 0.344 0.2079 0.3727 0.2611 0.2272 0.3216 0.2452 0.209 0.4635 0.3214 0.3925 0.4184 0.415 0.2037 0.556
Cytoplasmic Foci 0.0129 0.0041 0.0144 0.0105 0.012 0.0081 0.021 0.006 0.0104 0.0053 0.0219 0.0153 0.0216 0.0058 0.0085 0.0272 0.0138 0.0182
Eisosomes 0.0004 0.0001 0.0002 0.0001 0.0002 0.0001 0.001 0.0001 0.0001 0.0002 0.0004 0 0.0002 0.0001 0.0002 0.0003 0.0001 0.0001
Endoplasmic Reticulum 0.0025 0.002 0.0025 0.0032 0.0015 0.0013 0.0037 0.0012 0.0019 0.0011 0.0033 0.0014 0.0064 0.0022 0.0039 0.0057 0.0033 0.0041
Endosome 0.007 0.0013 0.0167 0.0142 0.032 0.0032 0.0135 0.0011 0.0028 0.0022 0.0206 0.0034 0.0083 0.0018 0.0054 0.0134 0.006 0.0193
Golgi 0.0029 0.0001 0.0019 0.0035 0.002 0.0011 0.0026 0.0001 0.0002 0.0005 0.0082 0.0002 0.004 0.0003 0.0018 0.0102 0.0004 0.0108
Lipid Particles 0.0035 0.0001 0.0018 0.0013 0.0041 0.0003 0.0039 0.0001 0.0006 0.001 0.0134 0.0003 0.0049 0.0004 0.0035 0.0068 0.001 0.0053
Mitochondria 0.0109 0.0002 0.0054 0.0339 0.0018 0.0011 0.0012 0.0004 0.0006 0.0008 0.0122 0.0004 0.0022 0.0001 0.0119 0.0133 0.0005 0.0035
None 0.6758 0.6423 0.6375 0.3172 0.3508 0.5555 0.6454 0.6622 0.5497 0.5028 0.2805 0.4476 0.5666 0.5792 0.4702 0.2212 0.3983 0.2916
Nuclear Periphery 0.0037 0.002 0.0056 0.0091 0.0065 0.0017 0.0044 0.001 0.0035 0.0062 0.0256 0.0017 0.0076 0.0011 0.0027 0.012 0.0048 0.004
Nucleolus 0.0007 0.0004 0.0009 0.0011 0.0041 0.0004 0.0007 0.0015 0.0007 0.0048 0.0041 0.0005 0.0003 0.0002 0.0004 0.0009 0.001 0.0006
Nucleus 0.0061 0.0454 0.0378 0.1959 0.2791 0.0328 0.0101 0.0519 0.0938 0.1749 0.3437 0.0428 0.0045 0.0111 0.0504 0.1904 0.3529 0.0526
Peroxisomes 0.0073 0.0001 0.0083 0.0017 0.0045 0.0032 0.0022 0.0001 0.0006 0.0008 0.0096 0.0005 0.0051 0.0001 0.0049 0.0199 0.0007 0.0083
Punctate Nuclear 0.0032 0.0024 0.0059 0.0313 0.0357 0.0063 0.0079 0.015 0.0098 0.0204 0.0271 0.0127 0.0019 0.0018 0.0075 0.0106 0.0099 0.0088
Vacuole 0.0019 0.0007 0.0043 0.0012 0.0111 0.0007 0.0027 0.0017 0.0009 0.0009 0.003 0.0007 0.0033 0.001 0.0015 0.0032 0.0009 0.0016
Vacuole Periphery 0.0004 0.0001 0.0009 0.0004 0.0012 0.0002 0.0007 0.0002 0.0002 0.0002 0.0034 0.0001 0.0012 0.0001 0.0008 0.0007 0.0002 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.8297 70.117 63.2796 52.4224 37.7923 4.2374 67.0631 91.1727 54.5108 30.9373
Translational Efficiency 0.8959 0.7607 1.0512 0.8034 0.6234 1.0319 0.9354 0.8767 1.0528 0.9012

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Nuclear protein putative transcription factor; required for growth of superficial pseudohyphae (which do not invade the agar substrate) but not for invasive pseudohyphal growth; may act together with Phd1p; potential Cdc28p substrate
Localization
Cell Percentages cytoplasm (22%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Sfg1

Sfg1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sfg1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available