Standard name
Description Vacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistance to cobalt and rhodium; protein abundance increases in response to DNA replication stress; COT1 has a paralog, ZRC1, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.09 0.12 0.16 0.15 0.22 0.34 0.25 0.08 0.07 0.05 0.18 0.09 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0.06 0 0.06 0.05 0.05 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.1 0 0 0.05 0 0 0 0 0 0
Mitochondria 0 0.06 0.07 0.09 0.05 0.13 0.09 0.1 0.23 0.26 0.2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.08 0.14 0.06 0 0 0.06 0 0 0 0 0 0.05 0 0.09 0.13 0.14 0.1 0 0.08 0 0.06 0.05 0
Nuclear Periphery 0.12 0 0.18 0.31 0.1 0.12 0.2 0.14 0.11 0.07 0.1 0.06 0 0 0.05 0.07 0.05 0.08 0 0.1 0.07 0.06 0.06
Nucleolus 0 0 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.82 0.81 0.8 0.81 0.84 0.71 0.62 0.68 0.55 0.48 0.49 0.84 0.85 0.85 0.75 0.83 0.78 0.81 0.85 0.81 0.8 0.84 0.82
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 2 0 1 1 1 4 2 1 1 1 0 0 0 0 0 0 1 0 0 0
Bud 0 0 0 0 0 0 0 0 1 3 5 0 0 2 0 0 0 0 1 3 1 2 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 1 1 2 3 17 6 21 13 21 1 3 0 1 1 1 0 0 0 0 0 0
Cytoplasm 3 6 18 17 37 51 94 94 136 156 140 22 28 20 20 23 21 0 0 0 0 0 2
Endoplasmic Reticulum 0 0 5 2 6 8 34 22 38 25 25 6 8 4 6 9 6 0 0 0 1 1 1
Endosome 1 1 9 4 14 1 6 6 0 1 8 0 1 1 2 2 3 0 2 10 5 11 9
Golgi 1 0 5 3 11 1 9 2 2 1 2 14 14 38 0 6 9 0 0 0 0 0 3
Mitochondria 5 9 29 31 21 55 53 66 139 119 112 1 1 1 4 6 0 3 2 7 5 6 7
Nucleus 9 20 24 5 13 23 19 18 15 5 13 14 18 33 14 35 17 5 12 14 25 30 25
Nuclear Periphery 14 4 78 102 40 50 122 90 70 31 58 17 15 5 5 18 8 10 2 45 28 35 34
Nucleolus 2 6 31 17 13 5 6 11 6 3 7 4 4 8 1 5 2 4 1 2 7 9 11
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 94 119 341 267 352 293 375 441 337 219 273 221 345 313 83 203 137 108 143 362 307 541 490
Unique Cell Count 114 147 428 331 419 410 604 646 609 460 555 264 405 368 111 244 176 134 170 450 385 642 597
Labelled Cell Count 130 165 541 451 509 492 736 758 769 578 666 301 438 425 136 308 204 134 170 450 385 642 597


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.4 16.5 13.8 12.7 11.4 9.4 9.1 9.0 7.8 7.8 7.8 21.5 27.0 32.5 11.2 12.4 15.5 18.0 17.1 19.2
Std Deviation (1e-4) 5.1 5.4 4.2 3.6 3.1 3.2 2.6 2.3 2.3 2.2 2.0 7.1 8.6 9.2 4.3 4.4 6.1 6.9 6.0 8.0
Intensity Change (Log2) -0.13 -0.28 -0.55 -0.61 -0.62 -0.83 -0.82 -0.83 0.64 0.97 1.23 -0.31 -0.16 0.17 0.38 0.31 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 3.1 0 3.5 3.1 3.7 0 0 0 0 0 0
Cytoplasm 0.6 2.7 4.3 5.8 5.4 8.1 11.1 8.9 2.3 1.7 0.8 5.1 2.7 3.5
Endoplasmic Reticulum 0 0.3 0.9 3.7 2.3 4.0 3.5 3.0 0 0.9 0 0 2.2 0
Endosome -0.9 1.1 0 -1.5 -1.6 0 0 -0.8 0 0 0 0 0 0
Golgi 0 1.6 0 0.4 0 0 0 0 3.2 2.2 5.7 0 0 0
Mitochondria 1.3 -1.1 3.2 1.2 1.9 6.9 7.6 5.9 -4.0 -5.1 -4.8 -1.2 -2.4 -3.5
Nucleus -2.9 -1.8 0 -1.9 -2.3 -2.6 -3.8 -2.7 -0.2 -0.8 1.8 2.6 3.8 1.8
Nuclear Periphery 4.0 -3.6 -2.4 0.8 -1.9 -3.1 -5.2 -3.5 -4.4 -6.7 -7.8 -3.6 -3.9 -4.4
Nucleolus -1.2 -2.7 -4.3 -5.3 -4.6 -5.3 -5.1 -4.8 -3.3 -4.5 -3.3 -2.5 -2.9 -3.0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.3 1.6 -2.8 -6.0 -4.1 -8.1 -9.9 -9.8 1.3 2.1 2.0 -1.1 1.1 -0.5


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cot1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available