Standard name
Human Ortholog
Description Protein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt5p, can instead interact with Pmt1p in some conditions; antifungal drug target; PMT3 has a paralog, PMT2, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.93 0.96 0.95 0.9 0.77 0.73 0.69 0.58 0.55 0.48 0.98 0.98 0.99 0.97 0.81 0.79 0.56 0.78 0.82 0.79
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.09 0 0.1 0.07 0.35 0.4 0.45 0.59 0.65 0.65 0 0 0 0 0.07 0.1 0.24 0.08 0.06 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 2 4 12 1 2 4
Bud 1 0 0 0 1 4 5 10 15 23 12 0 0 0 0 2 2 3 2 2 5
Bud Neck 0 2 1 0 0 1 3 1 1 0 1 0 0 1 0 2 2 3 2 3 4
Bud Site 0 0 0 0 0 0 2 2 4 0 4 0 0 0 0
Cell Periphery 2 1 1 3 4 3 4 1 5 2 2 0 0 0 4 0 2 5 1 1 2
Cytoplasm 274 249 317 245 314 354 421 475 330 297 228 59 135 207 295 232 227 197 103 207 188
Endoplasmic Reticulum 2 2 1 0 1 2 1 1 0 2 0 0 0 0 23 0 1 14 0 1 0
Endosome 0 0 3 3 14 3 7 7 2 1 7 0 0 1 2 3 4 7 2 1 4
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 1 0 2 1 0
Mitochondria 5 23 13 27 24 159 227 308 337 355 310 0 2 0 3 19 27 85 10 14 13
Nucleus 0 0 0 2 0 1 4 5 3 0 5 0 0 1 2 0 0 0 0 0 0
Nuclear Periphery 0 1 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 0 2 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 3 9 2 1 3 1 0 5 0 0 0 0 2 0 0 0 0 0
SpindlePole 0 0 1 3 5 4 12 10 11 16 24 0 0 0 0 4 3 4 0 5 2
Vac/Vac Membrane 0 1 2 2 7 10 8 5 1 8 6 0 0 0 4 3 3 6 3 4 7
Unique Cell Count 280 267 329 258 347 457 574 687 567 542 475 60 138 209 303 286 288 351 134 253 238
Labelled Cell Count 285 279 339 288 380 543 696 829 712 704 604 60 138 210 333 286 288 351 134 253 238


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 3.5 3.6 3.6 3.8 3.1 2.9 3.0 2.6 2.7 2.7 5.2 4.6 5.3 4.0 4.1 5.1 5.4 5.0 5.2
Std Deviation (1e-4) 0.9 0.6 1.2 1.7 1.8 1.8 1.2 1.4 1.0 1.0 0.8 2.0 0.8 1.5 1.0 0.9 1.3 3.1 1.5 1.9
Intensity Change (Log2) -0.0 0.09 -0.18 -0.3 -0.22 -0.47 -0.42 -0.38 0.55 0.37 0.56 0.16 0.22 0.51 0.59 0.49 0.55


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.6201 0.0907 -0.281 -0.4668 -0.371 -0.1241 1.6863 1.3575 1.3776 0.7649 0.8712 1.285 1.4395 1.5201 1.5223 1.0823 1.2963 1.2866
Actin 0.0026 0.001 0.0002 0.0003 0.0002 0.0002 0.1032 0.0002 0.0018 0.0004 0.0001 0.0046 0.063 0.0001 0.009 0.003 0.0002 0.0069
Bud 0.0001 0.0006 0.0041 0 0 0.0001 0.0007 0.0012 0.0009 0 0 0 0.0006 0.0002 0.0003 0.0087 0 0.0003
Bud Neck 0.0033 0.0001 0.0001 0 0 0.0001 0.0054 0.0002 0.0005 0 0 0 0.0038 0 0.0002 0.0002 0 0.0001
Bud Periphery 0.0002 0.0006 0.0042 0 0 0.0003 0.0011 0.0016 0.0029 0.0001 0 0.0001 0.0009 0.0004 0.0007 0.0126 0.0001 0.0011
Bud Site 0.0005 0.0127 0.0004 0 0 0 0.0091 0.0058 0.0145 0 0 0 0.0077 0.0003 0.0015 0.0016 0 0.0001
Cell Periphery 0.0001 0.0002 0.0001 0 0.0001 0 0.0005 0.0001 0.001 0.0001 0 0 0.0002 0.0001 0.0001 0.0001 0 0
Cytoplasm 0.0314 0.0529 0.0459 0.0209 0.045 0.0368 0.0197 0.0311 0.0395 0.0536 0.0576 0.0517 0.0546 0.0876 0.0682 0.0508 0.0806 0.0901
Cytoplasmic Foci 0.0134 0.0045 0.0024 0.0045 0.0058 0.0023 0.0159 0.0032 0.0047 0.002 0.0027 0.0027 0.0182 0.0022 0.0084 0.0058 0.0026 0.0039
Eisosomes 0.0003 0.0002 0.0002 0.0001 0.0002 0.0001 0.0006 0.0004 0.0003 0.0001 0.0001 0.0001 0.0005 0.0001 0.0002 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0018 0.0032 0.001 0.0015 0.0022 0.0045 0.002 0.0004 0.001 0.0019 0.0002 0.0004 0.0015 0.0007 0.0035 0.0003 0.0056 0.0005
Endosome 0.0033 0.0016 0.0006 0.0014 0.0027 0.0003 0.0195 0.0002 0.0118 0.0005 0.0003 0.0016 0.0153 0.0012 0.0044 0.0003 0.0003 0.0004
Golgi 0.0007 0.0003 0.0001 0.0001 0.0002 0 0.0068 0.0001 0.0036 0.0001 0 0.0008 0.0032 0 0.0016 0.0003 0 0.0002
Lipid Particles 0.0022 0.0006 0.0003 0.0008 0.0033 0.0001 0.0087 0.0002 0.0007 0.0004 0.0001 0.0005 0.0055 0.0001 0.0037 0.0009 0.0001 0.0002
Mitochondria 0.0002 0.0019 0.0025 0.0002 0.0008 0.0001 0.0022 0.0004 0.0082 0.0002 0.0001 0.0065 0.0028 0.0002 0.0013 0.0008 0.0001 0.0002
None 0.9355 0.9155 0.9359 0.966 0.9365 0.9537 0.7604 0.9509 0.9038 0.9393 0.9379 0.9251 0.777 0.9053 0.8782 0.9037 0.9092 0.8904
Nuclear Periphery 0.0004 0.0008 0.0003 0.0015 0.0006 0.0003 0.0078 0.0003 0.0004 0.0004 0.0001 0.0001 0.0034 0.0002 0.0003 0.0007 0.0003 0.0002
Nucleolus 0.0004 0.0005 0.0003 0.0001 0.0002 0.0001 0.004 0.0003 0.0002 0 0 0 0.0017 0.0001 0.0001 0.0006 0 0.0001
Nucleus 0.0006 0.0007 0.0004 0.0005 0.0004 0.0004 0.0016 0.0005 0.0004 0.0002 0.0001 0.0001 0.0071 0.0003 0.0003 0.0014 0.0002 0.0003
Peroxisomes 0.0005 0.0003 0.0001 0.0002 0.0003 0.0001 0.0085 0.0004 0.0007 0.0001 0 0.0049 0.0222 0.0002 0.0159 0.0041 0.0001 0.0007
Punctate Nuclear 0.0019 0.0011 0.0007 0.0014 0.0004 0.0004 0.0031 0.0019 0.0008 0.0002 0.0003 0.0002 0.0091 0.0003 0.0013 0.0035 0.0001 0.0042
Vacuole 0.0005 0.0006 0.0002 0.0003 0.0008 0.0001 0.0163 0.0004 0.0015 0.0003 0.0002 0.0002 0.0012 0.0004 0.0006 0.0002 0.0003 0.0001
Vacuole Periphery 0.0001 0.0002 0 0.0001 0.0001 0 0.003 0.0001 0.0008 0.0001 0 0.0001 0.0004 0 0.0002 0.0001 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 42.4386 27.472 17.1496 19.3485 27.5118 36.0772 25.352 18.8963 24.4652 43.4652
Translational Efficiency 0.8824 0.6813 0.5718 0.677 0.9882 1.1634 0.6799 0.6366 0.6097 0.6902

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
233 1775 100 1214 1617 1756 2511 1500 1850 3531 2611 2714

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 860.26 762.69 852.04 799.66 661.17 710.97 785.59 791.53 686.24 736.97 788.14 795.17
Standard Deviation 166.45 97.14 88.26 114.90 68.55 88.45 107.43 95.33 109.36 96.45 107.52 104.62
Intensity Change Log 2 -0.173676 -0.013852 -0.105386 0.104767 0.248755 0.259623 -0.046024 0.106181 0.064678

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002318 0.000223 0.000621 0.003366 0.000110 0.000334 0.000130 0.000281 0.000388 0.000278 0.000149 0.001661
Bud Neck 0.004287 0.029206 0.001039 0.012447 0.022443 0.054357 0.008123 0.007583 0.020156 0.041714 0.007851 0.009759
Bud Site 0.041875 0.019191 0.004146 0.050341 0.003610 0.016368 0.010070 0.031540 0.008429 0.017787 0.009843 0.039950
Cell Periphery 0.000297 0.000135 0.000332 0.000257 0.000126 0.000217 0.000111 0.000185 0.000148 0.000176 0.000119 0.000217
Cytoplasm 0.284485* 0.444294* 0.381711* 0.417401* 0.429442* 0.365424* 0.430994* 0.503629* 0.411185* 0.405072* 0.429107* 0.465059*
Cytoplasmic Foci 0.089391 0.209648* 0.046193 0.020480 0.271247* 0.299645* 0.006282 0.032283 0.248343* 0.254404* 0.007810 0.027004
Eisosomes 0.000106 0.000115 0.000115 0.000061 0.000229 0.000294 0.000017 0.000051 0.000214 0.000204 0.000021 0.000055
Endoplasmic Reticulum 0.003442 0.000585 0.006875 0.003355 0.000633 0.000596 0.001354 0.001336 0.000986 0.000591 0.001565 0.002239
Endosome 0.026197 0.018859 0.007148 0.007328 0.009817 0.026092 0.000686 0.005005 0.011880 0.022456 0.000933 0.006044
Golgi 0.019029 0.006006 0.000164 0.008943 0.002826 0.008664 0.000056 0.001415 0.004867 0.007328 0.000060 0.004783
Lipid Particles 0.003344 0.006002 0.004319 0.001905 0.011851 0.012237 0.000492 0.001516 0.010780 0.009103 0.000639 0.001690
Mitochondria 0.006552 0.007592 0.000518 0.003840 0.008122 0.026129 0.000679 0.002969 0.007924 0.016811 0.000673 0.003358
Mitotic Spindle 0.020306 0.013022 0.000190 0.035478 0.000451 0.003724 0.003877 0.015976 0.002951 0.008398 0.003736 0.024700
None 0.000716 0.002690 0.002599 0.004741 0.001948 0.002339 0.004696 0.003774 0.001793 0.002516 0.004616 0.004206
Nuclear Periphery 0.000762 0.000539 0.001064 0.002062 0.000443 0.000288 0.001666 0.001281 0.000483 0.000414 0.001643 0.001630
Nuclear Periphery Foci 0.001423 0.000493 0.006066 0.001857 0.000174 0.000196 0.000332 0.000732 0.000332 0.000345 0.000552 0.001235
Nucleolus 0.000393 0.001570 0.000923 0.000528 0.001058 0.001720 0.000365 0.000658 0.000974 0.001644 0.000386 0.000599
Nucleus 0.177703* 0.151253* 0.420381* 0.300134* 0.185170* 0.097567 0.507599* 0.308986* 0.184229* 0.124554 0.504259* 0.305026*
Peroxisomes 0.001751 0.011916 0.000723 0.002403 0.008637 0.024200 0.000124 0.001539 0.007770 0.018025 0.000147 0.001926
Vacuole 0.308257* 0.074531 0.114507 0.118890 0.040921 0.057140 0.022034 0.078059 0.074591 0.065883 0.025576 0.096323
Vacuole Periphery 0.007367 0.002129 0.000365 0.004184 0.000743 0.002468 0.000313 0.001201 0.001577 0.002298 0.000315 0.002535

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.27 0.82 0.02 -2.03 -1.29 -5.12 -1.32 -3.97 1.53 -2.58 0.18 0.95 -1.65 -1.90 -2.26
Bud Neck -13.04 4.69 -4.12 9.38 -6.95 -9.65 7.73 8.07 16.35 0.34 -9.41 7.44 7.33 18.72 -0.66
Bud Site 1.77 3.73 0.67 -2.16 -6.48 -7.71 -3.58 -7.24 -1.94 -5.14 -6.27 -0.47 -7.48 -2.83 -7.23
Cell Periphery 2.85 -1.97 1.13 -3.60 4.04 -5.62 1.82 -2.07 3.70 -3.18 -2.95 2.23 -2.75 0.23 -4.33
Cytoplasm -8.61 -2.42 -3.19 9.65 0.53 7.02 6.53 -0.84 -7.46 -7.00 0.88 4.56 1.96 1.27 -2.79
Cytoplasmic Foci -15.74 3.08 7.80 37.24 4.11 -3.62 47.21 42.23 47.90 -13.35 -1.26 45.67 42.30 59.06 -13.97
Eisosomes -1.79 -2.09 2.60 7.73 4.26 -3.19 16.03 13.21 15.04 -16.73 0.61 16.35 12.85 16.57 -20.91
Endoplasmic Reticulum 2.00 -3.23 -1.38 -17.06 3.08 0.28 -9.86 -10.82 -8.82 1.09 1.89 -6.99 -11.20 -18.72 -3.81
Endosome 0.27 4.04 5.26 11.38 2.83 -11.26 14.35 7.74 16.29 -6.54 -10.44 15.20 9.27 19.74 -8.61
Golgi 1.44 2.72 1.90 1.43 -2.65 -6.03 7.13 3.91 8.31 -2.15 -3.38 5.96 2.24 5.85 -3.27
Lipid Particles -4.22 -2.48 1.90 6.39 4.67 -0.41 15.91 14.52 13.81 -9.43 2.01 15.94 14.30 14.74 -10.80
Mitochondria -1.43 1.97 1.14 5.48 -2.38 -6.93 5.36 4.03 10.55 -1.74 -5.34 5.64 4.30 11.75 -2.68
Mitotic Spindle 0.67 3.03 -0.79 -2.45 -7.07 -3.40 -4.18 -6.34 -4.49 -4.20 -4.42 -1.44 -7.65 -4.36 -6.66
None -4.83 -4.97 -7.62 -1.89 -3.13 -0.87 -8.60 -4.13 -2.40 2.29 -2.13 -9.72 -5.85 -3.02 2.34
Nuclear Periphery 4.20 -1.41 -11.32 -15.24 -10.11 3.97 -16.17 -14.61 -16.00 1.98 2.24 -15.78 -20.85 -21.64 -0.88
Nuclear Periphery Foci 2.21 -4.60 -2.26 -7.75 3.96 -0.20 -4.58 -6.20 -6.13 -4.46 -0.38 -4.18 -9.72 -9.60 -6.68
Nucleolus -6.64 -3.00 -1.85 5.90 2.04 -1.59 3.62 2.39 3.67 -1.60 -2.46 3.51 2.56 6.12 -1.97
Nucleus 2.57 -6.20 -6.60 -20.94 2.58 16.36 -51.51 -19.10 -31.93 23.78 11.94 -50.34 -22.29 -36.79 27.49
Peroxisomes -10.27 1.87 0.35 10.07 -0.95 -9.17 12.90 10.14 14.61 -2.14 -9.30 13.03 10.49 17.64 -3.23
Vacuole 12.45 6.85 5.44 -17.92 -3.50 -5.91 1.92 -18.29 -14.70 -19.20 2.69 9.01 -16.43 -22.52 -28.15
Vacuole Periphery 1.35 2.20 1.47 0.45 -2.78 -6.54 4.98 0.05 5.24 -2.19 -2.75 3.65 0.18 3.04 -3.60