Standard name
Human Ortholog
Description Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.07 0 0 0.07 – 0.08 – 0.14 0.19 0.15 0.29 – 0 0 0 0.09 0.06 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 – 0 – 0.07 0.06 0.07 0.08 – 0 0 0 0.05 0.07 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.11 0.07 0.17 0.24 – 0.06 – 0.12 0.11 0.15 0.12 – 0.13 0.14 0.18 0.27 0.18 0.19 0.06 0 0 0 0 0
Mitochondria 0 0 0.06 0 – 0.07 – 0 0.09 0.05 0.08 – 0 0 0 0.09 0 0.06 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0.06 – 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0 0 0 0.05
Nuclear Periphery 0 0 0.06 0.05 – 0 – 0.05 0.06 0 0 – 0 0 0 0 0 0 0 0 0.1 0.09 0.07 0.06
Nucleolus 0 0 0.13 0.05 – 0.06 – 0 0 0 0 – 0 0 0 0.15 0.11 0.11 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.84 0.93 0.9 0.85 – 0.74 – 0.64 0.59 0.6 0.45 – 0.93 0.92 0.93 0.79 0.86 0.83 0.82 0.83 0.78 0.72 0.75 0.75
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 – 1 – 7 4 8 6 – 0 0 2 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 – 0 0 0 1 – 1 2 0 0 1 0 0 1 0 2 2 1
Bud Neck 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 – 2 – 6 2 11 6 – 1 0 3 0 1 1 0 0 0 0 0 0
Cytoplasm 4 12 2 14 – 18 – 47 50 48 59 – 8 6 9 21 18 25 2 7 1 1 2 1
Endoplasmic Reticulum 0 2 0 1 – 6 – 24 16 22 17 – 3 17 6 12 21 14 0 1 2 5 7 4
Endosome 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 1 1 3 1 5 5
Golgi 6 26 9 45 – 12 – 41 30 47 24 – 31 62 70 66 59 52 4 2 2 2 2 6
Mitochondria 2 1 3 3 – 16 – 9 25 15 17 – 0 1 2 21 13 16 0 1 3 3 3 1
Nucleus 0 15 1 2 – 13 – 2 4 2 1 – 2 3 1 7 6 2 2 28 5 5 8 10
Nuclear Periphery 0 0 3 9 – 6 – 19 16 10 5 – 2 3 2 0 0 1 0 15 22 15 16 13
Nucleolus 2 16 7 9 – 14 – 12 7 6 3 – 11 16 10 37 35 30 1 8 8 4 7 7
Peroxisomes 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 48 344 47 162 – 159 – 221 156 185 93 – 229 396 368 191 276 222 62 351 179 120 177 178
Unique Cell Count 57 371 52 191 216 347 264 310 205 247 430 396 243 321 269 76 422 231 167 238 236
Labelled Cell Count 62 416 72 247 247 388 310 354 232 288 506 473 355 430 363 76 422 231 167 238 236


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 75.6 71.4 53.2 51.9 – 49.5 – 48.4 50.8 47.7 44.3 – 64.2 67.9 65.5 65.9 73.0 67.6 71.5 74.1 84.8
Std Deviation (1e-4) 21.6 17.6 14.0 15.6 – 15.9 – 13.7 12.4 12.2 12.0 – 19.8 16.9 18.3 24.1 26.9 24.9 27.4 25.6 40.7
Intensity Change (Log2) – – – -0.04 – -0.1 – -0.14 -0.07 -0.16 -0.27 – 0.27 0.35 0.3 0.31 0.46 0.34 0.43 0.48 0.67


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Bud 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Cell Periphery 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm 0 – 0 – 2.0 2.7 2.3 3.8 – 0 0 0 0 0 0
Endoplasmic Reticulum 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Endosome 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Golgi 1.0 – 0 – -1.1 -1.2 -0.4 -1.1 – -0.9 -0.6 0.1 1.5 0.2 0.3
Mitochondria 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Nucleus 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Nuclear Periphery 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0 – 0 – 0 0 0 0 – 0 0 0 0.3 -0.5 -0.5
Peroxisomes 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Vacuole -1.0 – -2.6 – -3.8 -4.3 -4.3 -5.8 – 0.6 0.4 0.7 -2.0 -0.9 -1.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 38.3788 49.3117 44.9967 41.5149 42.2425 42.6498 45.0392 54.8867 49.8299 51.1864 38.9214 48.1186 54.125 64.018 59.2817 53.9029 55.9485 58.6377
Actin 0.0047 0 0.0009 0.0001 0.0003 0 0.0062 0.0001 0 0 0.0153 0 0.002 0 0 0 0 0
Bud 0.0008 0.0001 0.0009 0.0011 0 0 0.0003 0.0001 0.0001 0.0001 0.0007 0 0.0004 0 0.0001 0 0 0.0001
Bud Neck 0.0002 0 0.0002 0 0.0001 0 0.0002 0 0.0001 0 0.0002 0 0.0012 0 0 0 0 0
Bud Periphery 0.0021 0.0002 0.0007 0.0038 0.0001 0 0.0006 0.0001 0.0002 0.0002 0.0034 0.0001 0.0013 0.0001 0.0002 0.0001 0 0.0003
Bud Site 0.0009 0.0001 0.0025 0.0004 0.0001 0 0.0006 0.0001 0.0001 0 0.0005 0 0.0013 0 0 0 0 0
Cell Periphery 0.0026 0.002 0.0034 0.0009 0.0005 0.0005 0.0015 0.0017 0.0016 0.0027 0.003 0.0007 0.0029 0.0018 0.0033 0.0019 0.0016 0.0015
Cytoplasm 0.0072 0.0004 0.0059 0.0015 0.0066 0.0003 0.0052 0.0009 0.0002 0 0.0004 0 0.0015 0.0002 0.0001 0.0001 0.0001 0.0001
Cytoplasmic Foci 0.0126 0 0.0082 0.0051 0.0042 0.0041 0.0049 0.0001 0.0022 0 0.0206 0 0.0012 0 0 0.0005 0.0002 0
Eisosomes 0.0001 0 0 0 0 0 0.0001 0 0.0001 0 0.0002 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0018 0.0001 0.0005 0.0003 0.0008 0.0001 0.0043 0.0001 0.0001 0 0.0007 0.0001 0.0004 0.0001 0.0001 0 0 0
Endosome 0.0128 0.001 0.0109 0.004 0.0024 0.0026 0.0096 0.0008 0.002 0.0006 0.0084 0.0013 0.0026 0.0008 0.0012 0.0008 0.0005 0.0008
Golgi 0.0014 0 0.0035 0.0003 0.0002 0.0001 0.0023 0 0.0001 0 0.0172 0 0.0006 0 0 0.0001 0 0
Lipid Particles 0.0055 0.0003 0.0015 0.0025 0.0013 0.0025 0.0055 0.0005 0.0016 0.0003 0.0181 0.0015 0.0014 0.0005 0.0007 0.0016 0.001 0.0005
Mitochondria 0.0083 0.0005 0.0053 0.0057 0.0002 0.0024 0.0027 0.0014 0.0056 0.0001 0.0654 0.0002 0.0025 0.0002 0.0008 0.0068 0.0007 0.0005
None 0.0025 0 0.0043 0.0018 0.008 0.0001 0.003 0.0001 0.0003 0 0.0006 0 0.0004 0 0 0.0001 0.0001 0
Nuclear Periphery 0.0236 0.0065 0.0039 0.0062 0.0037 0.0035 0.0171 0.0067 0.0099 0.0024 0.0053 0.0056 0.0075 0.0221 0.0145 0.0028 0.0063 0.0149
Nucleolus 0.0126 0.0067 0.0066 0.0243 0.0015 0.0193 0.0013 0.0052 0.0061 0.0007 0.0204 0.0006 0.0077 0.0008 0.0051 0.0084 0.011 0.0024
Nucleus 0.0094 0.0024 0.0072 0.0027 0.0031 0.0019 0.0034 0.0019 0.0013 0.0006 0.0035 0.0005 0.0042 0.0021 0.0031 0.0013 0.0024 0.0018
Peroxisomes 0.0006 0 0.0039 0.0004 0.0001 0.0002 0.0015 0 0.0002 0 0.0069 0 0.0002 0 0 0.0002 0 0
Punctate Nuclear 0.0041 0 0.0013 0.0002 0.0117 0.0001 0.0042 0 0.0001 0 0.0002 0 0.0003 0 0 0 0 0
Vacuole 0.2339 0.2739 0.2631 0.2542 0.2357 0.1433 0.1592 0.1529 0.1852 0.1782 0.138 0.1383 0.2144 0.2476 0.2832 0.2214 0.1856 0.1554
Vacuole Periphery 0.6523 0.7057 0.6653 0.6841 0.7195 0.8189 0.7667 0.8272 0.783 0.8141 0.6711 0.8513 0.7461 0.7235 0.6875 0.7538 0.7902 0.8217

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 385.8772 434.1741 470.2309 388.0519 437.1315 449.2863 411.2896 422.382 436.4553 378.1307
Translational Efficiency 3.2258 2.9157 2.4809 3.3683 3.1597 2.7738 2.4582 2.8677 2.6172 2.667

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
262 967 2464 1492 1419 1421 114 864 1681 2388 2578 2356

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 3670.08 3889.99 4838.22 5719.40 2923.91 2824.27 6413.45 6770.30 3040.21 3255.82 4907.88 6104.79
Standard Deviation 806.06 984.08 1212.70 1924.36 1055.37 942.62 1928.69 2379.19 1055.81 1092.96 1294.21 2162.74
Intensity Change Log 2 — 0.083955 0.398665 0.640052 — -0.050021 1.133202 1.211321 — 0.026077 0.770916 0.921515

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000363 0.000030 0.000043 0.000172 0.000110 0.000087 0.000064 0.000284 0.000150 0.000064 0.000044 0.000213
Bud Neck 0.000117 0.000145 0.001071 0.002522 0.000305 0.000276 0.002246 0.004469 0.000276 0.000223 0.001123 0.003236
Bud Site 0.000122 0.000116 0.000778 0.004176 0.005041 0.001405 0.001264 0.005413 0.004275 0.000883 0.000800 0.004630
Cell Periphery 0.000679 0.000590 0.001176 0.001624 0.003756 0.002943 0.001340 0.002313 0.003277 0.001990 0.001184 0.001877
Cytoplasm 0.000043 0.000367 0.000235 0.001166 0.018538 0.001823 0.000390 0.000970 0.015655 0.001233 0.000242 0.001094
Cytoplasmic Foci 0.001282 0.000547 0.000557 0.003287 0.001890 0.001071 0.006400 0.006601 0.001795 0.000859 0.000816 0.004502
Eisosomes 0.000009 0.000006 0.000033 0.000068 0.000007 0.000009 0.000067 0.000314 0.000007 0.000008 0.000034 0.000158
Endoplasmic Reticulum 0.000053 0.000072 0.000187 0.000216 0.000392 0.000345 0.000068 0.000236 0.000339 0.000235 0.000181 0.000224
Endosome 0.000258 0.000183 0.000337 0.000935 0.001530 0.000969 0.000594 0.001900 0.001332 0.000651 0.000348 0.001289
Golgi 0.024011 0.004997 0.001283 0.003539 0.015413 0.009141 0.004128 0.004294 0.016753 0.007463 0.001409 0.003816
Lipid Particles 0.001025 0.000921 0.000536 0.000606 0.000760 0.000920 0.000658 0.001069 0.000801 0.000920 0.000541 0.000776
Mitochondria 0.002486 0.000636 0.000763 0.001972 0.003304 0.000937 0.000727 0.002437 0.003176 0.000815 0.000761 0.002143
Mitotic Spindle 0.000103 0.000016 0.000016 0.000469 0.000105 0.000114 0.000021 0.000829 0.000105 0.000074 0.000016 0.000601
None 0.000410 0.000193 0.000247 0.000443 0.004622 0.001399 0.000229 0.001354 0.003966 0.000911 0.000246 0.000777
Nuclear Periphery 0.001079 0.000452 0.001099 0.001419 0.000776 0.000191 0.000735 0.000878 0.000823 0.000297 0.001083 0.001221
Nuclear Periphery Foci 0.000926 0.000406 0.000443 0.000802 0.000430 0.000301 0.000652 0.000638 0.000507 0.000344 0.000452 0.000742
Nucleolus 0.000527 0.000553 0.005842 0.010021 0.001071 0.001753 0.022037 0.024502 0.000986 0.001267 0.006558 0.015332
Nucleus 0.000037 0.000190 0.001573 0.003522 0.004304 0.000949 0.001763 0.004429 0.003639 0.000642 0.001581 0.003855
Peroxisomes 0.000013 0.000008 0.000037 0.000219 0.000097 0.000042 0.000351 0.000454 0.000084 0.000028 0.000051 0.000305
Vacuole 0.065260 0.102653 0.301055* 0.316588* 0.105685 0.162512 0.342441* 0.386603* 0.099385 0.138272 0.302885* 0.342264*
Vacuole Periphery 0.901197* 0.886920* 0.682690* 0.646233* 0.831863* 0.812812* 0.613826* 0.550011* 0.842670* 0.842821* 0.679645* 0.610946*

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.99 0.95 0.52 -4.43 -4.19 0.84 1.89 -3.33 -3.62 -4.14 1.54 1.95 -1.27 -5.06 -6.20
Bud Neck -0.49 -5.67 -7.46 -7.32 -4.09 0.31 -3.03 -7.14 -7.23 -2.79 0.64 -4.91 -9.82 -10.19 -6.39
Bud Site 0.09 -5.43 -5.58 -5.59 -4.70 3.03 3.45 -0.52 -3.20 -3.53 3.52 3.80 -0.69 -5.53 -6.06
Cell Periphery 0.67 -3.08 -3.07 -3.53 -1.55 1.44 4.16 2.57 1.17 -1.91 3.01 5.51 3.15 0.21 -2.90
Cytoplasm -1.03 -2.99 -2.88 -1.84 -2.42 6.13 6.70 6.12 0.64 -1.03 6.27 6.73 6.14 -0.26 -2.55
Cytoplasmic Foci 1.58 1.67 -2.83 -4.26 -4.40 2.03 -1.84 -3.01 -3.54 -0.36 2.74 2.86 -3.65 -5.13 -5.18
Eisosomes 0.45 -4.58 -5.64 -6.26 -3.54 -1.09 -3.41 -3.00 -2.99 -2.39 -0.31 -8.45 -3.96 -3.95 -3.26
Endoplasmic Reticulum -0.90 -6.31 -4.75 -4.01 -0.90 0.47 6.87 2.16 1.01 -2.96 1.59 3.81 2.34 0.11 -1.35
Endosome 0.83 -0.98 -4.17 -5.07 -4.16 1.38 2.49 -0.52 -1.38 -1.99 2.13 3.34 0.06 -2.31 -3.75
Golgi 3.42 4.12 3.70 1.80 -8.92 3.15 6.31 6.07 2.92 -0.03 5.33 10.02 8.14 3.92 -5.71
Lipid Particles 0.29 1.64 1.32 1.42 -0.91 -0.51 0.47 -1.71 -0.49 -2.10 -0.53 2.03 0.08 0.65 -3.25
Mitochondria 1.74 1.59 0.34 -2.74 -2.39 3.22 3.63 1.03 -3.18 -3.95 3.79 3.79 1.36 -3.61 -3.57
Mitotic Spindle 1.36 1.36 -1.50 -1.88 -1.88 -0.10 2.74 -1.95 -1.90 -2.11 0.46 3.23 -2.46 -2.53 -2.83
None 3.05 1.35 -0.48 -2.78 -1.58 3.25 5.45 2.59 0.04 -1.15 4.00 5.41 4.13 0.24 -1.44
Nuclear Periphery 1.20 -0.07 -0.55 -2.36 -0.62 1.04 0.06 -0.19 -3.48 -0.49 1.09 -0.50 -0.76 -3.41 -0.34
Nuclear Periphery Foci 1.02 0.94 0.22 -2.20 -1.95 0.95 -1.18 -1.41 -3.20 0.08 1.19 0.36 -1.40 -3.33 -2.23
Nucleolus -0.10 -8.59 -7.22 -7.31 -2.98 -1.07 -2.87 -7.34 -7.18 -0.31 -0.56 -7.27 -9.66 -9.80 -5.62
Nucleus -1.62 -7.43 -5.48 -5.11 -3.00 4.04 2.66 -0.19 -2.93 -2.13 4.32 2.85 -0.46 -5.33 -3.72
Peroxisomes 1.10 -3.88 -2.90 -2.99 -2.57 1.13 -1.60 -3.44 -4.40 -0.61 1.37 0.80 -3.23 -4.90 -4.48
Vacuole -4.94 -30.23 -28.91 -29.95 -2.23 -9.33 -10.27 -29.44 -22.78 -1.77 -8.13 -36.38 -40.06 -34.49 -6.00
Vacuole Periphery 1.39 20.81 22.39* 30.42* 4.87 2.08 8.64 24.49 24.13 2.52 -0.14 22.01 29.13 34.85* 9.96
Standard name
Human Ortholog
Description Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages vacuolar membrane (72%)
Cell Cycle Regulation No
Subcompartmental Group vac memb-2

Vma4

Vma4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vma4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available