Standard name
Description Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.07 0 0 0.07 – 0.08 – 0.14 0.19 0.15 0.29 – 0 0 0 0.09 0.06 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 – 0 – 0.07 0.06 0.07 0.08 – 0 0 0 0.05 0.07 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.11 0.07 0.17 0.24 – 0.06 – 0.12 0.11 0.15 0.12 – 0.13 0.14 0.18 0.27 0.18 0.19 0.06 0 0 0 0 0
Mitochondria 0 0 0.06 0 – 0.07 – 0 0.09 0.05 0.08 – 0 0 0 0.09 0 0.06 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0.06 – 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0 0 0 0.05
Nuclear Periphery 0 0 0.06 0.05 – 0 – 0.05 0.06 0 0 – 0 0 0 0 0 0 0 0 0.1 0.09 0.07 0.06
Nucleolus 0 0 0.13 0.05 – 0.06 – 0 0 0 0 – 0 0 0 0.15 0.11 0.11 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.84 0.93 0.9 0.85 – 0.74 – 0.64 0.59 0.6 0.45 – 0.93 0.92 0.93 0.79 0.86 0.83 0.82 0.83 0.78 0.72 0.75 0.75
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 – 1 – 7 4 8 6 – 0 0 2 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 – 0 0 0 1 – 1 2 0 0 1 0 0 1 0 2 2 1
Bud Neck 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 – 2 – 6 2 11 6 – 1 0 3 0 1 1 0 0 0 0 0 0
Cytoplasm 4 12 2 14 – 18 – 47 50 48 59 – 8 6 9 21 18 25 2 7 1 1 2 1
Endoplasmic Reticulum 0 2 0 1 – 6 – 24 16 22 17 – 3 17 6 12 21 14 0 1 2 5 7 4
Endosome 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 1 1 3 1 5 5
Golgi 6 26 9 45 – 12 – 41 30 47 24 – 31 62 70 66 59 52 4 2 2 2 2 6
Mitochondria 2 1 3 3 – 16 – 9 25 15 17 – 0 1 2 21 13 16 0 1 3 3 3 1
Nucleus 0 15 1 2 – 13 – 2 4 2 1 – 2 3 1 7 6 2 2 28 5 5 8 10
Nuclear Periphery 0 0 3 9 – 6 – 19 16 10 5 – 2 3 2 0 0 1 0 15 22 15 16 13
Nucleolus 2 16 7 9 – 14 – 12 7 6 3 – 11 16 10 37 35 30 1 8 8 4 7 7
Peroxisomes 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 48 344 47 162 – 159 – 221 156 185 93 – 229 396 368 191 276 222 62 351 179 120 177 178
Unique Cell Count 57 371 52 191 216 347 264 310 205 247 430 396 243 321 269 76 422 231 167 238 236
Labelled Cell Count 62 416 72 247 247 388 310 354 232 288 506 473 355 430 363 76 422 231 167 238 236


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 75.6 71.4 53.2 51.9 – 49.5 – 48.4 50.8 47.7 44.3 – 64.2 67.9 65.5 65.9 73.0 67.6 71.5 74.1 84.8
Std Deviation (1e-4) 21.6 17.6 14.0 15.6 – 15.9 – 13.7 12.4 12.2 12.0 – 19.8 16.9 18.3 24.1 26.9 24.9 27.4 25.6 40.7
Intensity Change (Log2) – – – -0.04 – -0.1 – -0.14 -0.07 -0.16 -0.27 – 0.27 0.35 0.3 0.31 0.46 0.34 0.43 0.48 0.67


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Bud 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Cell Periphery 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm 0 – 0 – 2.0 2.7 2.3 3.8 – 0 0 0 0 0 0
Endoplasmic Reticulum 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Endosome 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Golgi 1.0 – 0 – -1.1 -1.2 -0.4 -1.1 – -0.9 -0.6 0.1 1.5 0.2 0.3
Mitochondria 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Nucleus 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Nuclear Periphery 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0 – 0 – 0 0 0 0 – 0 0 0 0.3 -0.5 -0.5
Peroxisomes 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Vacuole -1.0 – -2.6 – -3.8 -4.3 -4.3 -5.8 – 0.6 0.4 0.7 -2.0 -0.9 -1.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 38.3788 49.3117 44.9967 41.5149 42.2425 42.6498 45.0392 54.8867 49.8299 51.1864 38.9214 48.1186 54.125 64.018 59.2817 53.9029 55.9485 58.6377
Actin 0.0047 0 0.0009 0.0001 0.0003 0 0.0062 0.0001 0 0 0.0153 0 0.002 0 0 0 0 0
Bud 0.0008 0.0001 0.0009 0.0011 0 0 0.0003 0.0001 0.0001 0.0001 0.0007 0 0.0004 0 0.0001 0 0 0.0001
Bud Neck 0.0002 0 0.0002 0 0.0001 0 0.0002 0 0.0001 0 0.0002 0 0.0012 0 0 0 0 0
Bud Periphery 0.0021 0.0002 0.0007 0.0038 0.0001 0 0.0006 0.0001 0.0002 0.0002 0.0034 0.0001 0.0013 0.0001 0.0002 0.0001 0 0.0003
Bud Site 0.0009 0.0001 0.0025 0.0004 0.0001 0 0.0006 0.0001 0.0001 0 0.0005 0 0.0013 0 0 0 0 0
Cell Periphery 0.0026 0.002 0.0034 0.0009 0.0005 0.0005 0.0015 0.0017 0.0016 0.0027 0.003 0.0007 0.0029 0.0018 0.0033 0.0019 0.0016 0.0015
Cytoplasm 0.0072 0.0004 0.0059 0.0015 0.0066 0.0003 0.0052 0.0009 0.0002 0 0.0004 0 0.0015 0.0002 0.0001 0.0001 0.0001 0.0001
Cytoplasmic Foci 0.0126 0 0.0082 0.0051 0.0042 0.0041 0.0049 0.0001 0.0022 0 0.0206 0 0.0012 0 0 0.0005 0.0002 0
Eisosomes 0.0001 0 0 0 0 0 0.0001 0 0.0001 0 0.0002 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0018 0.0001 0.0005 0.0003 0.0008 0.0001 0.0043 0.0001 0.0001 0 0.0007 0.0001 0.0004 0.0001 0.0001 0 0 0
Endosome 0.0128 0.001 0.0109 0.004 0.0024 0.0026 0.0096 0.0008 0.002 0.0006 0.0084 0.0013 0.0026 0.0008 0.0012 0.0008 0.0005 0.0008
Golgi 0.0014 0 0.0035 0.0003 0.0002 0.0001 0.0023 0 0.0001 0 0.0172 0 0.0006 0 0 0.0001 0 0
Lipid Particles 0.0055 0.0003 0.0015 0.0025 0.0013 0.0025 0.0055 0.0005 0.0016 0.0003 0.0181 0.0015 0.0014 0.0005 0.0007 0.0016 0.001 0.0005
Mitochondria 0.0083 0.0005 0.0053 0.0057 0.0002 0.0024 0.0027 0.0014 0.0056 0.0001 0.0654 0.0002 0.0025 0.0002 0.0008 0.0068 0.0007 0.0005
None 0.0025 0 0.0043 0.0018 0.008 0.0001 0.003 0.0001 0.0003 0 0.0006 0 0.0004 0 0 0.0001 0.0001 0
Nuclear Periphery 0.0236 0.0065 0.0039 0.0062 0.0037 0.0035 0.0171 0.0067 0.0099 0.0024 0.0053 0.0056 0.0075 0.0221 0.0145 0.0028 0.0063 0.0149
Nucleolus 0.0126 0.0067 0.0066 0.0243 0.0015 0.0193 0.0013 0.0052 0.0061 0.0007 0.0204 0.0006 0.0077 0.0008 0.0051 0.0084 0.011 0.0024
Nucleus 0.0094 0.0024 0.0072 0.0027 0.0031 0.0019 0.0034 0.0019 0.0013 0.0006 0.0035 0.0005 0.0042 0.0021 0.0031 0.0013 0.0024 0.0018
Peroxisomes 0.0006 0 0.0039 0.0004 0.0001 0.0002 0.0015 0 0.0002 0 0.0069 0 0.0002 0 0 0.0002 0 0
Punctate Nuclear 0.0041 0 0.0013 0.0002 0.0117 0.0001 0.0042 0 0.0001 0 0.0002 0 0.0003 0 0 0 0 0
Vacuole 0.2339 0.2739 0.2631 0.2542 0.2357 0.1433 0.1592 0.1529 0.1852 0.1782 0.138 0.1383 0.2144 0.2476 0.2832 0.2214 0.1856 0.1554
Vacuole Periphery 0.6523 0.7057 0.6653 0.6841 0.7195 0.8189 0.7667 0.8272 0.783 0.8141 0.6711 0.8513 0.7461 0.7235 0.6875 0.7538 0.7902 0.8217

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 385.8772 434.1741 470.2309 388.0519 437.1315 449.2863 411.2896 422.382 436.4553 378.1307
Translational Efficiency 3.2258 2.9157 2.4809 3.3683 3.1597 2.7738 2.4582 2.8677 2.6172 2.667

Hit Data

Dataset Hit
Protein Abundance ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘