Standard name
Description Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.07 0 0 0.07 – 0.08 – 0.14 0.19 0.15 0.29 – 0 0 0 0.09 0.06 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 – 0 – 0.07 0.06 0.07 0.08 – 0 0 0 0.05 0.07 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.11 0.07 0.17 0.24 – 0.06 – 0.12 0.11 0.15 0.12 – 0.13 0.14 0.18 0.27 0.18 0.19 0.06 0 0 0 0 0
Mitochondria 0 0 0.06 0 – 0.07 – 0 0.09 0.05 0.08 – 0 0 0 0.09 0 0.06 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0.06 – 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0 0 0 0.05
Nuclear Periphery 0 0 0.06 0.05 – 0 – 0.05 0.06 0 0 – 0 0 0 0 0 0 0 0 0.1 0.09 0.07 0.06
Nucleolus 0 0 0.13 0.05 – 0.06 – 0 0 0 0 – 0 0 0 0.15 0.11 0.11 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.84 0.93 0.9 0.85 – 0.74 – 0.64 0.59 0.6 0.45 – 0.93 0.92 0.93 0.79 0.86 0.83 0.82 0.83 0.78 0.72 0.75 0.75
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 – 1 – 7 4 8 6 – 0 0 2 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 – 0 0 0 1 – 1 2 0 0 1 0 0 1 0 2 2 1
Bud Neck 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 – 2 – 6 2 11 6 – 1 0 3 0 1 1 0 0 0 0 0 0
Cytoplasm 4 12 2 14 – 18 – 47 50 48 59 – 8 6 9 21 18 25 2 7 1 1 2 1
Endoplasmic Reticulum 0 2 0 1 – 6 – 24 16 22 17 – 3 17 6 12 21 14 0 1 2 5 7 4
Endosome 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 1 1 3 1 5 5
Golgi 6 26 9 45 – 12 – 41 30 47 24 – 31 62 70 66 59 52 4 2 2 2 2 6
Mitochondria 2 1 3 3 – 16 – 9 25 15 17 – 0 1 2 21 13 16 0 1 3 3 3 1
Nucleus 0 15 1 2 – 13 – 2 4 2 1 – 2 3 1 7 6 2 2 28 5 5 8 10
Nuclear Periphery 0 0 3 9 – 6 – 19 16 10 5 – 2 3 2 0 0 1 0 15 22 15 16 13
Nucleolus 2 16 7 9 – 14 – 12 7 6 3 – 11 16 10 37 35 30 1 8 8 4 7 7
Peroxisomes 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 48 344 47 162 – 159 – 221 156 185 93 – 229 396 368 191 276 222 62 351 179 120 177 178
Unique Cell Count 57 371 52 191 216 347 264 310 205 247 430 396 243 321 269 76 422 231 167 238 236
Labelled Cell Count 62 416 72 247 247 388 310 354 232 288 506 473 355 430 363 76 422 231 167 238 236


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 75.6 71.4 53.2 51.9 – 49.5 – 48.4 50.8 47.7 44.3 – 64.2 67.9 65.5 65.9 73.0 67.6 71.5 74.1 84.8
Std Deviation (1e-4) 21.6 17.6 14.0 15.6 – 15.9 – 13.7 12.4 12.2 12.0 – 19.8 16.9 18.3 24.1 26.9 24.9 27.4 25.6 40.7
Intensity Change (Log2) – – – -0.04 – -0.1 – -0.14 -0.07 -0.16 -0.27 – 0.27 0.35 0.3 0.31 0.46 0.34 0.43 0.48 0.67


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Bud 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Cell Periphery 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm 0 – 0 – 2.0 2.7 2.3 3.8 – 0 0 0 0 0 0
Endoplasmic Reticulum 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Endosome 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Golgi 1.0 – 0 – -1.1 -1.2 -0.4 -1.1 – -0.9 -0.6 0.1 1.5 0.2 0.3
Mitochondria 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Nucleus 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Nuclear Periphery 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0 – 0 – 0 0 0 0 – 0 0 0 0.3 -0.5 -0.5
Peroxisomes 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Vacuole -1.0 – -2.6 – -3.8 -4.3 -4.3 -5.8 – 0.6 0.4 0.7 -2.0 -0.9 -1.4


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vma4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available