Standard name | |
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Description | Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | – | 1 | – | 7 | 4 | 8 | 6 | – | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | 1 | – | 1 | 2 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 2 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 1 | – | 2 | – | 6 | 2 | 11 | 6 | – | 1 | 0 | 3 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 12 | 2 | 14 | – | 18 | – | 47 | 50 | 48 | 59 | – | 8 | 6 | 9 | 21 | 18 | 25 | 2 | 7 | 1 | 1 | 2 | 1 |
Endoplasmic Reticulum | 0 | 2 | 0 | 1 | – | 6 | – | 24 | 16 | 22 | 17 | – | 3 | 17 | 6 | 12 | 21 | 14 | 0 | 1 | 2 | 5 | 7 | 4 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 1 | 5 | 5 |
Golgi | 6 | 26 | 9 | 45 | – | 12 | – | 41 | 30 | 47 | 24 | – | 31 | 62 | 70 | 66 | 59 | 52 | 4 | 2 | 2 | 2 | 2 | 6 |
Mitochondria | 2 | 1 | 3 | 3 | – | 16 | – | 9 | 25 | 15 | 17 | – | 0 | 1 | 2 | 21 | 13 | 16 | 0 | 1 | 3 | 3 | 3 | 1 |
Nucleus | 0 | 15 | 1 | 2 | – | 13 | – | 2 | 4 | 2 | 1 | – | 2 | 3 | 1 | 7 | 6 | 2 | 2 | 28 | 5 | 5 | 8 | 10 |
Nuclear Periphery | 0 | 0 | 3 | 9 | – | 6 | – | 19 | 16 | 10 | 5 | – | 2 | 3 | 2 | 0 | 0 | 1 | 0 | 15 | 22 | 15 | 16 | 13 |
Nucleolus | 2 | 16 | 7 | 9 | – | 14 | – | 12 | 7 | 6 | 3 | – | 11 | 16 | 10 | 37 | 35 | 30 | 1 | 8 | 8 | 4 | 7 | 7 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 48 | 344 | 47 | 162 | – | 159 | – | 221 | 156 | 185 | 93 | – | 229 | 396 | 368 | 191 | 276 | 222 | 62 | 351 | 179 | 120 | 177 | 178 |
Unique Cell Count | 57 | 371 | 52 | 191 | 216 | 347 | 264 | 310 | 205 | 247 | 430 | 396 | 243 | 321 | 269 | 76 | 422 | 231 | 167 | 238 | 236 | |||
Labelled Cell Count | 62 | 416 | 72 | 247 | 247 | 388 | 310 | 354 | 232 | 288 | 506 | 473 | 355 | 430 | 363 | 76 | 422 | 231 | 167 | 238 | 236 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 75.6 | 71.4 | 53.2 | 51.9 | – | 49.5 | – | 48.4 | 50.8 | 47.7 | 44.3 | – | 64.2 | 67.9 | 65.5 | 65.9 | 73.0 | 67.6 | 71.5 | 74.1 | 84.8 |
Std Deviation (1e-4) | 21.6 | 17.6 | 14.0 | 15.6 | – | 15.9 | – | 13.7 | 12.4 | 12.2 | 12.0 | – | 19.8 | 16.9 | 18.3 | 24.1 | 26.9 | 24.9 | 27.4 | 25.6 | 40.7 |
Intensity Change (Log2) | – | – | – | -0.04 | – | -0.1 | – | -0.14 | -0.07 | -0.16 | -0.27 | – | 0.27 | 0.35 | 0.3 | 0.31 | 0.46 | 0.34 | 0.43 | 0.48 | 0.67 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
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Cortical Patches | 0 | – | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | – | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | – | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | – | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | – | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | – | 0 | – | 2.0 | 2.7 | 2.3 | 3.8 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | – | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | – | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 1.0 | – | 0 | – | -1.1 | -1.2 | -0.4 | -1.1 | – | -0.9 | -0.6 | 0.1 | 1.5 | 0.2 | 0.3 |
Mitochondria | 0 | – | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | – | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | – | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | – | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0.3 | -0.5 | -0.5 |
Peroxisomes | 0 | – | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | – | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.0 | – | -2.6 | – | -3.8 | -4.3 | -4.3 | -5.8 | – | 0.6 | 0.4 | 0.7 | -2.0 | -0.9 | -1.4 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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