Standard name | |
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Description | Cytoplasmic and mitochondrial alanyl-tRNA synthetase; required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog AARS; mutations in human homolog AARS are associated with autoimmune disease polymyositis/dermatomyositis |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 7 | – | 7 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Bud | 2 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 1 | – | 5 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 2 | 1 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 0 | 4 | 7 | 9 | 13 | 32 | 21 | 23 | – | 21 | 27 | 1 | 1 | 5 | 4 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 249 | 96 | 319 | 363 | 435 | 371 | 538 | 400 | 337 | – | 303 | 377 | 241 | 394 | 407 | 361 | 202 | 205 | 234 | 108 | 309 | 164 | 208 | 304 |
Endoplasmic Reticulum | 0 | 0 | 6 | 2 | 13 | 16 | 53 | 46 | 29 | – | 43 | 51 | 4 | 1 | 0 | 18 | 12 | 16 | 0 | 2 | 5 | 1 | 9 | 23 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 2 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | – | 0 | 0 | 0 | 2 | 2 | 7 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 2 |
Unique Cell Count | 249 | 97 | 324 | 365 | 446 | 388 | 597 | 457 | 382 | 360 | 443 | 245 | 396 | 412 | 369 | 203 | 205 | 237 | 116 | 322 | 171 | 229 | 338 | |
Labelled Cell Count | 253 | 97 | 330 | 375 | 457 | 400 | 624 | 473 | 401 | 380 | 468 | 246 | 399 | 416 | 391 | 215 | 225 | 237 | 116 | 322 | 171 | 229 | 338 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 111.4 | 93.9 | 70.6 | 67.3 | 71.2 | 68.2 | 68.7 | 64.0 | 58.3 | – | 60.5 | 57.6 | 99.3 | 89.1 | 74.4 | 99.7 | 122.8 | 121.9 | 93.0 | 88.2 | 98.2 |
Std Deviation (1e-4) | 19.7 | 23.0 | 12.6 | 12.9 | 13.2 | 16.2 | 15.8 | 15.2 | 16.0 | – | 17.8 | 15.9 | 18.9 | 19.6 | 16.9 | 32.3 | 32.7 | 38.5 | 22.9 | 27.8 | 26.0 |
Intensity Change (Log2) | – | – | – | -0.07 | 0.01 | -0.05 | -0.04 | -0.14 | -0.28 | – | -0.22 | -0.29 | 0.49 | 0.33 | 0.07 | 0.5 | 0.8 | 0.79 | 0.4 | 0.32 | 0.48 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.7 | 0.8 | 1.8 | 3.1 | 2.6 | 3.3 | – | 3.2 | 3.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.3 | -0.9 | -2.2 | -4.8 | -5.6 | -5.3 | – | -6.5 | -6.3 | -0.1 | 1.4 | 0.4 | -0.6 | 1.1 | 1.8 |
Endoplasmic Reticulum | 0 | 0.9 | 1.7 | 4.2 | 4.5 | 3.5 | – | 5.1 | 5.0 | 0 | 0 | 0 | 2.2 | 2.5 | 3.3 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |