Standard name
Description Cytoplasmic and mitochondrial alanyl-tRNA synthetase; required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog AARS; mutations in human homolog AARS are associated with autoimmune disease polymyositis/dermatomyositis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0.05 0.05 0.06 – 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.98 0.99 0.98 0.96 0.9 0.88 0.88 – 0.84 0.85 0.98 0.99 0.99 0.98 1.0 1.0 0.99 0.94 0.96 0.96 0.91 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0.09 0.1 0.08 – 0.12 0.12 0 0 0 0.05 0.06 0.08 0 0 0 0 0 0.07
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 1 2 7 – 7 9 0 0 0 0 0 0 0 0 0 1 0 0
Bud 2 0 0 3 0 0 0 0 1 – 5 1 0 1 0 0 0 1 0 0 2 2 1 3
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 0 4 7 9 13 32 21 23 – 21 27 1 1 5 4 1 3 0 0 0 0 0 0
Cytoplasm 249 96 319 363 435 371 538 400 337 – 303 377 241 394 407 361 202 205 234 108 309 164 208 304
Endoplasmic Reticulum 0 0 6 2 13 16 53 46 29 – 43 51 4 1 0 18 12 16 0 2 5 1 9 23
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 1 2 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 1 – 0 2 0 0 2 0 0 0 0 1 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 1 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 0 0 0 0 0 3 0 – 0 0 0 2 2 7 0 0 0 0 2 0 2 2
Unique Cell Count 249 97 324 365 446 388 597 457 382 360 443 245 396 412 369 203 205 237 116 322 171 229 338
Labelled Cell Count 253 97 330 375 457 400 624 473 401 380 468 246 399 416 391 215 225 237 116 322 171 229 338


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 111.4 93.9 70.6 67.3 71.2 68.2 68.7 64.0 58.3 – 60.5 57.6 99.3 89.1 74.4 99.7 122.8 121.9 93.0 88.2 98.2
Std Deviation (1e-4) 19.7 23.0 12.6 12.9 13.2 16.2 15.8 15.2 16.0 – 17.8 15.9 18.9 19.6 16.9 32.3 32.7 38.5 22.9 27.8 26.0
Intensity Change (Log2) – – – -0.07 0.01 -0.05 -0.04 -0.14 -0.28 – -0.22 -0.29 0.49 0.33 0.07 0.5 0.8 0.79 0.4 0.32 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0.7 0.8 1.8 3.1 2.6 3.3 – 3.2 3.4 0 0 0 0 0 0
Cytoplasm 1.3 -0.9 -2.2 -4.8 -5.6 -5.3 – -6.5 -6.3 -0.1 1.4 0.4 -0.6 1.1 1.8
Endoplasmic Reticulum 0 0.9 1.7 4.2 4.5 3.5 – 5.1 5.0 0 0 0 2.2 2.5 3.3
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0