Standard name | |
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Description | Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 |
Bud | 0 | 0 | 3 | 4 | 0 | 5 | 0 | 7 | 3 | 4 | 0 | 3 | 0 | 1 | 1 | 0 | 0 | 0 | 2 | 5 | 1 | 2 | 2 | 0 |
Bud Neck | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 1 | 1 |
Bud Site | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 2 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 202 | 476 | 336 | 193 | 224 | 255 | 398 | 475 | 362 | 455 | 185 | 234 | 258 | 284 | 308 | 263 | 128 | 164 | 37 | 73 | 67 | 34 | 31 | 44 |
Endoplasmic Reticulum | 2 | 3 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 1 | 0 | 19 | 7 | 5 | 2 | 3 | 2 | 3 | 4 | 6 |
Endosome | 12 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 2 | 20 | 7 | 18 | 11 | 13 | 4 | 1 | 4 | 4 |
Golgi | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 2 | 2 | 0 | 0 | 3 | 1 |
Mitochondria | 2 | 9 | 12 | 15 | 10 | 25 | 75 | 77 | 104 | 104 | 65 | 69 | 0 | 1 | 3 | 11 | 3 | 7 | 7 | 14 | 17 | 5 | 8 | 12 |
Nucleus | 11 | 6 | 0 | 0 | 1 | 0 | 11 | 3 | 2 | 2 | 2 | 1 | 0 | 0 | 1 | 24 | 9 | 10 | 1 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 1 | 0 | 0 | 1 | 4 | 5 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 3 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 119 | 200 | 36 | 2 | 8 | 15 | 30 | 32 | 22 | 49 | 15 | 28 | 3 | 2 | 2 | 187 | 104 | 125 | 249 | 520 | 286 | 144 | 148 | 161 |
Unique Cell Count | 287 | 615 | 374 | 199 | 236 | 274 | 460 | 525 | 431 | 518 | 229 | 276 | 264 | 289 | 315 | 409 | 212 | 261 | 323 | 653 | 391 | 198 | 212 | 239 |
Labelled Cell Count | 350 | 703 | 394 | 214 | 244 | 304 | 520 | 599 | 496 | 619 | 271 | 335 | 265 | 291 | 319 | 526 | 262 | 331 | 323 | 653 | 391 | 198 | 212 | 239 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.5 | 4.1 | 3.4 | 3.4 | 3.7 | 3.4 | 3.3 | 3.4 | 2.9 | 3.0 | 3.0 | 3.0 | 4.9 | 4.5 | 3.9 | 6.7 | 7.4 | 6.9 | 4.9 | 5.6 | 5.4 |
Std Deviation (1e-4) | 0.7 | 0.7 | 0.6 | 1.1 | 0.9 | 0.7 | 0.8 | 1.0 | 0.4 | 0.8 | 0.4 | 0.9 | 5.0 | 1.2 | 1.1 | 1.9 | 2.0 | 2.0 | 1.8 | 2.2 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.0 | 0.12 | 0.01 | -0.04 | -0.01 | -0.22 | -0.17 | -0.2 | -0.21 | 0.53 | 0.41 | 0.2 | 0.99 | 1.12 | 1.02 | 0.53 | 0.71 | 0.66 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 11.2 | 10.8 | 10.9 |