Standard name
Description Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.7 0.77 0.9 0.97 0.95 0.93 0.87 0.9 0.84 0.88 0.81 0.85 0.98 0.98 0.98 0.64 0.6 0.63 0.12 0.11 0.17 0.18 0.15 0.19
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.07 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.08 0 0.09 0.16 0.15 0.24 0.2 0.28 0.25 0 0 0 0 0 0 0 0 0 0 0 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.41 0.33 0.1 0 0 0.05 0.07 0.06 0.05 0.09 0.07 0.1 0 0 0 0.46 0.49 0.48 0.77 0.8 0.73 0.73 0.7 0.67
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 2 0
Bud 0 0 3 4 0 5 0 7 3 4 0 3 0 1 1 0 0 0 2 5 1 2 2 0
Bud Neck 0 0 4 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 3 0 1 1
Bud Site 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 0 0 1 2 0 1 2 0 0 0 1 1 0 0 0 0 0 0 0 0 0
Cytoplasm 202 476 336 193 224 255 398 475 362 455 185 234 258 284 308 263 128 164 37 73 67 34 31 44
Endoplasmic Reticulum 2 3 1 0 0 1 0 0 0 1 3 0 0 1 0 19 7 5 2 3 2 3 4 6
Endosome 12 6 0 0 0 0 0 0 0 0 0 0 3 1 2 20 7 18 11 13 4 1 4 4
Golgi 2 1 0 0 1 0 0 0 0 0 0 0 0 0 0 1 3 0 2 2 0 0 3 1
Mitochondria 2 9 12 15 10 25 75 77 104 104 65 69 0 1 3 11 3 7 7 14 17 5 8 12
Nucleus 11 6 0 0 1 0 11 3 2 2 2 1 0 0 1 24 9 10 1 1 0 0 0 0
Nuclear Periphery 0 1 1 0 0 1 4 5 0 1 1 0 1 0 1 0 0 0 0 1 0 0 0 0
Nucleolus 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 2 1 3 0 0 0 1
Vac/Vac Membrane 119 200 36 2 8 15 30 32 22 49 15 28 3 2 2 187 104 125 249 520 286 144 148 161
Unique Cell Count 287 615 374 199 236 274 460 525 431 518 229 276 264 289 315 409 212 261 323 653 391 198 212 239
Labelled Cell Count 350 703 394 214 244 304 520 599 496 619 271 335 265 291 319 526 262 331 323 653 391 198 212 239


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 4.1 3.4 3.4 3.7 3.4 3.3 3.4 2.9 3.0 3.0 3.0 4.9 4.5 3.9 6.7 7.4 6.9 4.9 5.6 5.4
Std Deviation (1e-4) 0.7 0.7 0.6 1.1 0.9 0.7 0.8 1.0 0.4 0.8 0.4 0.9 5.0 1.2 1.1 1.9 2.0 2.0 1.8 2.2 1.5
Intensity Change (Log2) – – – 0.0 0.12 0.01 -0.04 -0.01 -0.22 -0.17 -0.2 -0.21 0.53 0.41 0.2 0.99 1.12 1.02 0.53 0.71 0.66


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 11.2 10.8 10.9