Standard name | |
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Human Ortholog | |
Description | Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 |
Bud | 0 | 0 | 3 | 4 | 0 | 5 | 0 | 7 | 3 | 4 | 0 | 3 | 0 | 1 | 1 | 0 | 0 | 0 | 2 | 5 | 1 | 2 | 2 | 0 |
Bud Neck | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 1 | 1 |
Bud Site | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 2 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 202 | 476 | 336 | 193 | 224 | 255 | 398 | 475 | 362 | 455 | 185 | 234 | 258 | 284 | 308 | 263 | 128 | 164 | 37 | 73 | 67 | 34 | 31 | 44 |
Endoplasmic Reticulum | 2 | 3 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 1 | 0 | 19 | 7 | 5 | 2 | 3 | 2 | 3 | 4 | 6 |
Endosome | 12 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 2 | 20 | 7 | 18 | 11 | 13 | 4 | 1 | 4 | 4 |
Golgi | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 2 | 2 | 0 | 0 | 3 | 1 |
Mitochondria | 2 | 9 | 12 | 15 | 10 | 25 | 75 | 77 | 104 | 104 | 65 | 69 | 0 | 1 | 3 | 11 | 3 | 7 | 7 | 14 | 17 | 5 | 8 | 12 |
Nucleus | 11 | 6 | 0 | 0 | 1 | 0 | 11 | 3 | 2 | 2 | 2 | 1 | 0 | 0 | 1 | 24 | 9 | 10 | 1 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 1 | 0 | 0 | 1 | 4 | 5 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 3 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 119 | 200 | 36 | 2 | 8 | 15 | 30 | 32 | 22 | 49 | 15 | 28 | 3 | 2 | 2 | 187 | 104 | 125 | 249 | 520 | 286 | 144 | 148 | 161 |
Unique Cell Count | 287 | 615 | 374 | 199 | 236 | 274 | 460 | 525 | 431 | 518 | 229 | 276 | 264 | 289 | 315 | 409 | 212 | 261 | 323 | 653 | 391 | 198 | 212 | 239 |
Labelled Cell Count | 350 | 703 | 394 | 214 | 244 | 304 | 520 | 599 | 496 | 619 | 271 | 335 | 265 | 291 | 319 | 526 | 262 | 331 | 323 | 653 | 391 | 198 | 212 | 239 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.5 | 4.1 | 3.4 | 3.4 | 3.7 | 3.4 | 3.3 | 3.4 | 2.9 | 3.0 | 3.0 | 3.0 | 4.9 | 4.5 | 3.9 | 6.7 | 7.4 | 6.9 | 4.9 | 5.6 | 5.4 |
Std Deviation (1e-4) | 0.7 | 0.7 | 0.6 | 1.1 | 0.9 | 0.7 | 0.8 | 1.0 | 0.4 | 0.8 | 0.4 | 0.9 | 5.0 | 1.2 | 1.1 | 1.9 | 2.0 | 2.0 | 1.8 | 2.2 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.0 | 0.12 | 0.01 | -0.04 | -0.01 | -0.22 | -0.17 | -0.2 | -0.21 | 0.53 | 0.41 | 0.2 | 0.99 | 1.12 | 1.02 | 0.53 | 0.71 | 0.66 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 11.2 | 10.8 | 10.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.1197 | 1.1288 | 0.5763 | 0.046 | 0.0136 | 0.6431 | 4.5601 | 3.929 | 4.2928 | 3.5922 | 4.0212 | 4.4909 | 3.8856 | 3.6577 | 4.041 | 3.5363 | 3.616 | 3.6369 |
Actin | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0334 | 0.0002 | 0.0117 | 0.0005 | 0.0009 | 0.0042 | 0.0221 | 0.0054 | 0.0118 | 0.0039 | 0.0211 | 0.005 |
Bud | 0 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0022 | 0.0043 | 0.0052 | 0.0006 | 0.0005 | 0.002 | 0.0029 | 0.0029 | 0.0021 | 0.0065 | 0.02 | 0.0062 |
Bud Neck | 0.0001 | 0.0002 | 0.0002 | 0.0005 | 0.0005 | 0.0006 | 0.0092 | 0.0069 | 0.015 | 0.003 | 0.0044 | 0.0055 | 0.0014 | 0.0053 | 0.0013 | 0.0005 | 0.0008 | 0.0016 |
Bud Periphery | 0 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0035 | 0.0068 | 0.0084 | 0.0012 | 0.0009 | 0.004 | 0.0022 | 0.0015 | 0.0028 | 0.0057 | 0.0057 | 0.0047 |
Bud Site | 0.0002 | 0.0006 | 0.0005 | 0.0006 | 0.0002 | 0.0002 | 0.0173 | 0.0119 | 0.0181 | 0.0057 | 0.0024 | 0.0011 | 0.027 | 0.0148 | 0.0076 | 0.0015 | 0.0017 | 0.0013 |
Cell Periphery | 0.0002 | 0.0004 | 0.0004 | 0.0003 | 0.0001 | 0.0002 | 0.0011 | 0.0008 | 0.0007 | 0.0005 | 0.0016 | 0.0005 | 0.0009 | 0.0012 | 0.0005 | 0.0004 | 0.0003 | 0.0003 |
Cytoplasm | 0.1736 | 0.3534 | 0.3336 | 0.4366 | 0.3365 | 0.5117 | 0.1067 | 0.2501 | 0.1794 | 0.1619 | 0.1734 | 0.269 | 0.1134 | 0.2429 | 0.1599 | 0.0924 | 0.2681 | 0.2939 |
Cytoplasmic Foci | 0.0109 | 0.0156 | 0.018 | 0.0317 | 0.0426 | 0.0142 | 0.0427 | 0.0279 | 0.0483 | 0.0582 | 0.0572 | 0.0504 | 0.0545 | 0.0438 | 0.0362 | 0.1073 | 0.0221 | 0.0509 |
Eisosomes | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0009 | 0.0002 | 0.0005 | 0.0002 | 0.0002 | 0.0003 |
Endoplasmic Reticulum | 0.0035 | 0.004 | 0.0042 | 0.0033 | 0.0022 | 0.004 | 0.0052 | 0.0044 | 0.0043 | 0.0047 | 0.0017 | 0.005 | 0.0072 | 0.0046 | 0.003 | 0.0031 | 0.0017 | 0.0036 |
Endosome | 0.0183 | 0.0199 | 0.0247 | 0.0608 | 0.0904 | 0.0164 | 0.0457 | 0.0541 | 0.0596 | 0.1033 | 0.1125 | 0.058 | 0.0601 | 0.0411 | 0.0268 | 0.2077 | 0.0615 | 0.0599 |
Golgi | 0.0003 | 0.0003 | 0.0005 | 0.0014 | 0.001 | 0.0003 | 0.0086 | 0.0009 | 0.0081 | 0.004 | 0.0173 | 0.0034 | 0.0061 | 0.0021 | 0.0022 | 0.0035 | 0.0039 | 0.0016 |
Lipid Particles | 0.0014 | 0.0009 | 0.0012 | 0.0007 | 0.0027 | 0.0009 | 0.0168 | 0.0016 | 0.0132 | 0.0267 | 0.0107 | 0.0028 | 0.0245 | 0.0058 | 0.0048 | 0.0049 | 0.0014 | 0.0043 |
Mitochondria | 0.0002 | 0.0004 | 0.0006 | 0.0011 | 0.0008 | 0.0005 | 0.0084 | 0.0015 | 0.0126 | 0.0032 | 0.0171 | 0.0087 | 0.0024 | 0.0015 | 0.0046 | 0.0083 | 0.016 | 0.002 |
None | 0.7701 | 0.5604 | 0.595 | 0.3876 | 0.4925 | 0.4121 | 0.575 | 0.527 | 0.5379 | 0.408 | 0.4574 | 0.477 | 0.6177 | 0.5474 | 0.6929 | 0.3759 | 0.5127 | 0.5084 |
Nuclear Periphery | 0.001 | 0.0018 | 0.0013 | 0.001 | 0.0007 | 0.0016 | 0.0227 | 0.0025 | 0.0068 | 0.0052 | 0.0016 | 0.0029 | 0.015 | 0.0021 | 0.0027 | 0.0125 | 0.001 | 0.0023 |
Nucleolus | 0.0002 | 0.0004 | 0.0003 | 0.0004 | 0.0003 | 0.0004 | 0.0022 | 0.0025 | 0.0023 | 0.0046 | 0.0019 | 0.0017 | 0.0017 | 0.0053 | 0.0013 | 0.003 | 0.0005 | 0.0008 |
Nucleus | 0.001 | 0.003 | 0.0029 | 0.0019 | 0.0017 | 0.0046 | 0.0217 | 0.008 | 0.005 | 0.006 | 0.0063 | 0.0065 | 0.0025 | 0.009 | 0.0028 | 0.0058 | 0.0058 | 0.0027 |
Peroxisomes | 0.0001 | 0.0002 | 0.0003 | 0.0003 | 0.0003 | 0.0002 | 0.0307 | 0.0004 | 0.0089 | 0.0295 | 0.0264 | 0.0083 | 0.009 | 0.0021 | 0.0041 | 0.0035 | 0.0067 | 0.001 |
Punctate Nuclear | 0.0006 | 0.001 | 0.001 | 0.0005 | 0.0005 | 0.0021 | 0.0079 | 0.0024 | 0.0055 | 0.0052 | 0.0014 | 0.0032 | 0.0053 | 0.011 | 0.0054 | 0.0068 | 0.001 | 0.0026 |
Vacuole | 0.0169 | 0.035 | 0.0131 | 0.0678 | 0.024 | 0.0281 | 0.0343 | 0.0818 | 0.0447 | 0.1574 | 0.0987 | 0.0812 | 0.0204 | 0.0479 | 0.0241 | 0.1339 | 0.0447 | 0.0415 |
Vacuole Periphery | 0.0009 | 0.002 | 0.001 | 0.0029 | 0.0024 | 0.0013 | 0.0044 | 0.004 | 0.0041 | 0.0105 | 0.0056 | 0.0046 | 0.0027 | 0.0023 | 0.0025 | 0.0129 | 0.0033 | 0.0049 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 17.2323 | 11.5535 | 16.0747 | 17.8281 | 12.3931 | 7.2658 | 16.0015 | 19.2181 | 17.1482 | 19.4683 |
Translational Efficiency | 0.5929 | 0.6763 | 0.6363 | 0.5016 | 0.5368 | 1.241 | 0.6118 | 0.3604 | 0.4536 | 0.4663 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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