Standard name
Description Ty1 enhancer activator involved in Ty enhancer-mediated transcription; required for full levels of Ty enhancer-mediated transcription; C6 zinc cluster DNA-binding protein
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0.06 0.06 0.06 0.05 0.06 0.05 0.05 0.07 0.11 0.07 0.1 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.12 0 0.09 0.05 0.3 0.41 0.2 0.63 0.7 0.71 0.48 0.08 0 0 0 0 0.06 0 0 0 0 0 0
Nucleus 0.95 0.96 0.95 0.92 0.92 0.83 0.78 0.79 0.66 0.62 0.6 0.69 0.92 0.9 0.83 0.83 0.8 0.76 0.91 0.94 0.9 0.82 0.7 0.64
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.08 0.07 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0.07 0.1 0.09
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0.05 0.07 0 0 0.05 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0.08 0 0 0 0.06 0.11 0.16
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 0 0 2 1 3 3 3 7 6 8 8 0 0 0 0 0 0 0 1 0 0 3 5
Bud Neck 0 0 1 0 3 0 5 7 5 4 0 7 0 0 0 0 0 0 0 0 0 1 0 2
Bud Site 0 1 0 2 6 12 10 3 16 11 17 8 1 0 0 0 1 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 2 7 13 14 19 27 30 23 26 29 17 20 27 43 14 18 13 0 0 0 2 1 1
Endoplasmic Reticulum 0 0 1 0 0 0 2 0 0 0 0 0 0 1 0 0 2 4 0 0 0 1 2 1
Endosome 1 0 1 0 1 1 0 4 0 0 0 1 0 1 0 4 0 5 2 0 3 1 3 5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 3 3 0 1 2 2 0
Mitochondria 16 27 11 25 19 137 198 101 260 331 326 179 32 9 17 4 5 7 2 0 4 1 4 6
Nucleus 356 211 363 267 336 384 379 408 274 294 276 257 349 344 337 161 142 97 350 222 360 224 250 214
Nuclear Periphery 0 0 1 1 3 1 0 0 2 0 2 0 2 1 1 1 0 0 0 0 0 0 1 2
Nucleolus 31 16 6 7 6 7 5 8 17 5 13 9 11 20 26 2 3 2 11 3 9 19 37 32
Peroxisomes 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 9 3 13 12 22 36 18 15 23 25 0 0 0 0 1 0 1 0 1 0 3 3
Vac/Vac Membrane 3 0 2 3 2 2 0 7 7 3 3 6 7 3 12 12 13 10 9 5 14 16 40 54
Unique Cell Count 373 219 381 291 365 460 485 517 413 474 458 375 381 382 404 194 177 127 384 237 399 275 357 338
Labelled Cell Count 412 257 402 323 404 578 651 607 630 696 698 517 423 406 436 201 185 141 384 237 399 275 357 338


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.6 6.0 6.2 5.5 5.7 4.4 4.2 4.5 3.7 3.6 3.4 4.0 6.3 6.2 6.0 9.2 9.1 9.3 6.2 6.4 6.8
Std Deviation (1e-4) 1.5 1.1 0.9 1.1 1.0 1.1 0.9 0.9 1.1 0.9 0.8 0.8 1.5 1.7 1.8 1.9 1.9 2.0 1.6 1.8 1.9
Intensity Change (Log2) – – – -0.15 -0.1 -0.49 -0.55 -0.45 -0.74 -0.76 -0.86 -0.63 0.02 0.02 -0.03 0.57 0.56 0.59 0.01 0.05 0.15


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.0 1.6 1.9 2.8 3.0 2.8 2.8 3.2 2.1 2.5 3.5 5.0 3.3 4.4 4.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.3 1.6 0 0 0 0 0 0 0 3.3 -0.5 1.0 -0.6 0 0
Nucleus -1.9 -1.8 -5.4 -7.1 -7.0 -10.2 -11.5 -11.9 -9.6 -2.0 -2.8 -5.3 -4.9 -5.6 -6.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.8 0.1 0 0 0 0 0 0 0 1.2 2.8 3.4 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole -1.3 1.0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 2.6 0 0 0