ORF
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOR342C has a paralog, YAL037W, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.12 0.25 0.33 0.4 0.95 0.85 0.81 0.83 0.73 0.72 0.64 0.68 0.69 0.94 0.98 0.53 0.48 0.46 0.13 0.11 0.24 0.86 0.86 0.82
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.1 0.1 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.23 0 0 0.09 0 0.2 0.33 0.22 0.44 0.35 0.49 0.39 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.37 0.4 0.52 0.36 0 0 0 0 0 0 0 0 0.15 0 0 0.09 0.11 0.07 0.66 0.63 0.5 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.5 0.6 0.25 0.26 0.05 0.06 0 0 0 0 0 0 0.27 0.08 0 0.47 0.42 0.47 0.11 0.12 0.14 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 2 4 10
Bud 0 0 0 0 0 3 5 2 10 7 3 3 0 0 0 0 0 0 5 8 4 2 10 14
Bud Neck 0 0 2 1 2 2 5 8 3 8 3 12 0 0 1 0 0 0 0 1 1 1 1 3
Bud Site 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0
Cell Periphery 0 0 0 0 3 1 0 1 0 1 0 0 0 1 0 0 0 1 0 0 0 0 1 2
Cytoplasm 14 50 72 81 334 263 333 373 188 258 153 191 164 412 457 79 78 66 17 24 59 243 343 342
Endoplasmic Reticulum 0 2 0 0 1 1 0 0 0 1 0 1 1 1 1 4 10 8 0 0 1 0 3 1
Endosome 0 1 1 2 2 2 1 1 0 0 0 0 7 1 2 16 16 14 1 4 3 5 4 5
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 1 0 1 0 0 1 0 1
Mitochondria 26 5 0 18 8 61 137 101 114 124 117 109 0 0 1 0 1 2 0 4 3 5 9 12
Nucleus 42 80 112 73 2 5 3 9 4 9 4 2 37 5 1 13 18 10 86 140 125 3 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 1 0 1 1 2 0 0 1 0 1 0 1 1 1 0 1 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 3 3 2 2 9 1 0 0 4 5 3 2 6 10 3 8 10
Vac/Vac Membrane 57 121 53 53 17 20 6 10 1 12 3 8 64 35 15 70 68 68 14 27 36 6 3 4
Unique Cell Count 114 201 215 201 352 308 413 449 257 356 239 280 239 437 465 149 163 144 131 224 251 284 399 417
Labelled Cell Count 139 259 241 228 371 358 493 509 325 422 285 337 275 457 478 187 198 173 131 224 251 284 399 417


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 7.2 6.3 5.4 4.9 3.9 3.4 4.1 3.3 3.4 3.2 3.4 6.1 5.8 5.6 8.2 7.9 7.5 5.4 5.3 5.7
Std Deviation (1e-4) 0.9 1.2 1.1 1.3 1.3 1.3 1.0 1.3 1.5 1.2 1.6 1.1 1.1 1.0 1.2 1.7 2.0 1.8 1.6 1.3 1.5
Intensity Change (Log2) -0.2 -0.36 -0.68 -0.86 -0.62 -0.9 -0.87 -0.96 -0.88 -0.03 -0.11 -0.16 0.4 0.34 0.26 -0.22 -0.24 -0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.4 15.7 12.2 11.7 12.7 8.6 9.1 6.5 7.7 7.5 16.7 18.9 3.7 2.8 2.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 4.6 4.3 4.3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.5 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus -3.2 -14.9 -13.6 -15.8 -15.6 -12.7 -14.0 -12.3 -13.5 -8.3 -15.9 -16.9 -8.6 -8.3 -8.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.4 -7.0 -5.9 -9.5 -9.2 -8.2 -7.8 -7.6 -7.3 0.5 -5.8 -8.7 4.4 3.5 4.4