ORF
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOR342C has a paralog, YAL037W, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.12 0.25 0.33 0.4 0.95 0.85 0.81 0.83 0.73 0.72 0.64 0.68 0.69 0.94 0.98 0.53 0.48 0.46 0.13 0.11 0.24 0.86 0.86 0.82
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.1 0.1 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.23 0 0 0.09 0 0.2 0.33 0.22 0.44 0.35 0.49 0.39 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.37 0.4 0.52 0.36 0 0 0 0 0 0 0 0 0.15 0 0 0.09 0.11 0.07 0.66 0.63 0.5 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.5 0.6 0.25 0.26 0.05 0.06 0 0 0 0 0 0 0.27 0.08 0 0.47 0.42 0.47 0.11 0.12 0.14 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 2 4 10
Bud 0 0 0 0 0 3 5 2 10 7 3 3 0 0 0 0 0 0 5 8 4 2 10 14
Bud Neck 0 0 2 1 2 2 5 8 3 8 3 12 0 0 1 0 0 0 0 1 1 1 1 3
Bud Site 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0
Cell Periphery 0 0 0 0 3 1 0 1 0 1 0 0 0 1 0 0 0 1 0 0 0 0 1 2
Cytoplasm 14 50 72 81 334 263 333 373 188 258 153 191 164 412 457 79 78 66 17 24 59 243 343 342
Endoplasmic Reticulum 0 2 0 0 1 1 0 0 0 1 0 1 1 1 1 4 10 8 0 0 1 0 3 1
Endosome 0 1 1 2 2 2 1 1 0 0 0 0 7 1 2 16 16 14 1 4 3 5 4 5
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 1 0 1 0 0 1 0 1
Mitochondria 26 5 0 18 8 61 137 101 114 124 117 109 0 0 1 0 1 2 0 4 3 5 9 12
Nucleus 42 80 112 73 2 5 3 9 4 9 4 2 37 5 1 13 18 10 86 140 125 3 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 1 0 1 1 2 0 0 1 0 1 0 1 1 1 0 1 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 3 3 2 2 9 1 0 0 4 5 3 2 6 10 3 8 10
Vac/Vac Membrane 57 121 53 53 17 20 6 10 1 12 3 8 64 35 15 70 68 68 14 27 36 6 3 4
Unique Cell Count 114 201 215 201 352 308 413 449 257 356 239 280 239 437 465 149 163 144 131 224 251 284 399 417
Labelled Cell Count 139 259 241 228 371 358 493 509 325 422 285 337 275 457 478 187 198 173 131 224 251 284 399 417


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 7.2 6.3 5.4 4.9 3.9 3.4 4.1 3.3 3.4 3.2 3.4 6.1 5.8 5.6 8.2 7.9 7.5 5.4 5.3 5.7
Std Deviation (1e-4) 0.9 1.2 1.1 1.3 1.3 1.3 1.0 1.3 1.5 1.2 1.6 1.1 1.1 1.0 1.2 1.7 2.0 1.8 1.6 1.3 1.5
Intensity Change (Log2) -0.2 -0.36 -0.68 -0.86 -0.62 -0.9 -0.87 -0.96 -0.88 -0.03 -0.11 -0.16 0.4 0.34 0.26 -0.22 -0.24 -0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.4 15.7 12.2 11.7 12.7 8.6 9.1 6.5 7.7 7.5 16.7 18.9 3.7 2.8 2.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 4.6 4.3 4.3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.5 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus -3.2 -14.9 -13.6 -15.8 -15.6 -12.7 -14.0 -12.3 -13.5 -8.3 -15.9 -16.9 -8.6 -8.3 -8.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.4 -7.0 -5.9 -9.5 -9.2 -8.2 -7.8 -7.6 -7.3 0.5 -5.8 -8.7 4.4 3.5 4.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.6414 1.3852 1.1765 0.8555 0.4969 1.1266 1.9181 2.3013 1.8654 1.0068 0.7128 1.6782 2.094 2.4998 2.2905 1.7598 1.415 2.0435
Actin 0.0402 0.0003 0.0153 0.0016 0.0191 0.0032 0.0127 0.0001 0.0146 0.0002 0.0004 0.0021 0.0001 0.0001 0.0003 0.0003 0.0004 0.0002
Bud 0.0012 0.0024 0.0006 0.0004 0.0008 0.0013 0.0033 0.0003 0.0008 0.0002 0.0002 0.0047 0.0005 0.0001 0.0012 0.0008 0.0007 0.001
Bud Neck 0.0033 0.0006 0.0016 0.001 0.0044 0.0049 0.0071 0.0005 0.0007 0.0011 0.0021 0.0026 0.0004 0.0004 0.0004 0.0008 0.0015 0.0023
Bud Periphery 0.0015 0.0006 0.0005 0.0003 0.0011 0.0015 0.0007 0.0001 0.0019 0.0002 0.0001 0.0008 0.0005 0 0.0002 0.0002 0.0003 0.0003
Bud Site 0.0026 0.0025 0.0017 0.001 0.0016 0.0004 0.0043 0.0018 0.0013 0.0002 0.0003 0.0002 0.0005 0.0004 0.0008 0.0003 0.0009 0.0003
Cell Periphery 0.0002 0 0.0001 0 0.0001 0.0001 0.0001 0 0.0001 0 0 0 0 0 0.0001 0 0 0
Cytoplasm 0.138 0.2711 0.1307 0.1984 0.2598 0.1458 0.113 0.2188 0.1064 0.1484 0.1622 0.1086 0.1152 0.1891 0.1312 0.2003 0.2324 0.1282
Cytoplasmic Foci 0.0246 0.0023 0.0071 0.016 0.0142 0.007 0.0114 0.0018 0.0076 0.0006 0.0017 0.003 0.0043 0.0011 0.003 0.0027 0.0047 0.0063
Eisosomes 0.0002 0 0.0002 0 0.0002 0.0001 0.0002 0 0.0001 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0058 0.0027 0.0027 0.0062 0.0084 0.0025 0.0065 0.0034 0.0018 0.0014 0.0162 0.0014 0.0022 0.0031 0.0023 0.0034 0.0026 0.0014
Endosome 0.0421 0.003 0.0207 0.0421 0.0339 0.011 0.0263 0.0023 0.0074 0.0012 0.0081 0.0041 0.003 0.0026 0.0038 0.0078 0.0069 0.0032
Golgi 0.0131 0.0001 0.0088 0.0027 0.0057 0.0027 0.0031 0 0.0022 0 0.0001 0.0006 0.0001 0 0.0001 0.0001 0.0002 0.0001
Lipid Particles 0.0208 0.0001 0.0065 0.0084 0.005 0.0064 0.0043 0.0001 0.0039 0 0.0002 0.0017 0.0002 0.0001 0.0003 0.0001 0.0001 0.0002
Mitochondria 0.0102 0.0005 0.011 0.0029 0.0033 0.0037 0.0017 0.0004 0.0144 0.0004 0.0019 0.001 0.0015 0.0003 0.0018 0.0004 0.0007 0.0003
None 0.0496 0.045 0.0551 0.1094 0.1439 0.0562 0.0266 0.0613 0.06 0.0022 0.0134 0.0196 0.0352 0.0143 0.0214 0.0073 0.0683 0.0144
Nuclear Periphery 0.0432 0.0223 0.0184 0.0383 0.0418 0.0181 0.027 0.0146 0.0136 0.0159 0.0338 0.0121 0.0135 0.0214 0.0126 0.0185 0.0185 0.0099
Nucleolus 0.0027 0.0018 0.0024 0.0019 0.0074 0.0029 0.0048 0.0015 0.0017 0.0015 0.0057 0.0023 0.002 0.0012 0.0033 0.0022 0.0023 0.0023
Nucleus 0.5424 0.6246 0.6721 0.5434 0.376 0.6864 0.7103 0.68 0.7109 0.8166 0.7267 0.8088 0.8051 0.7539 0.7995 0.7192 0.6288 0.7915
Peroxisomes 0.0161 0.0001 0.0091 0.0027 0.0016 0.0012 0.0025 0.0001 0.0065 0 0.0001 0.0014 0.0003 0 0.0001 0.0001 0.0002 0.0001
Punctate Nuclear 0.0261 0.0173 0.0317 0.017 0.0652 0.0419 0.0302 0.0108 0.0406 0.0081 0.0237 0.024 0.0128 0.0097 0.0161 0.0337 0.0265 0.0367
Vacuole 0.0112 0.0023 0.0025 0.0046 0.0045 0.0018 0.0029 0.0017 0.0024 0.0015 0.0021 0.001 0.0021 0.0016 0.0012 0.0015 0.0035 0.0011
Vacuole Periphery 0.0049 0.0005 0.001 0.0016 0.0021 0.001 0.001 0.0003 0.0011 0.0003 0.001 0.0004 0.0004 0.0004 0.0003 0.0004 0.0006 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 39.6536 13.3572 33.547 51.0112 34.2751 17.2311 8.0513 10.5176 19.2154 49.0076
Translational Efficiency 0.5057 0.6795 0.4746 0.4425 0.7204 1.0447 0.582 0.2718 0.6713 0.5781

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
274 527 942 1730 1267 1491 1728 1253 1541 2018 2670 2983

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 820.36 902.65 1088.20 1147.46 733.44 855.84 1167.69 1124.96 748.89 868.06 1139.65 1138.01
Standard Deviation 100.58 121.43 153.06 212.74 99.95 117.57 162.87 190.82 105.44 120.36 163.94 204.13
Intensity Change Log 2 0.137910 0.407615 0.484115 0.222662 0.670906 0.617123 0.178536 0.537896 0.548429

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000134 0.001080 0.000611 0.000780 0.000154 0.000421 0.000695 0.001119 0.000150 0.000593 0.000665 0.000922
Bud Neck 0.001554 0.003614 0.004149 0.010794 0.001566 0.006064 0.004644 0.009606 0.001564 0.005424 0.004469 0.010295
Bud Site 0.000868 0.002966 0.002901 0.005256 0.000970 0.006154 0.001321 0.010130 0.000952 0.005321 0.001879 0.007303
Cell Periphery 0.000142 0.000127 0.000109 0.000158 0.000127 0.000143 0.000094 0.000138 0.000129 0.000139 0.000100 0.000150
Cytoplasm 0.168973* 0.170518* 0.052639 0.063559 0.267200* 0.166961* 0.032105 0.081334 0.249735* 0.167890* 0.039350 0.071025
Cytoplasmic Foci 0.045958 0.017856 0.000413 0.001471 0.026656 0.013272 0.001145 0.005867 0.030088 0.014469 0.000887 0.003318
Eisosomes 0.000054 0.000030 0.000018 0.000016 0.000032 0.000022 0.000015 0.000017 0.000036 0.000024 0.000016 0.000016
Endoplasmic Reticulum 0.003472 0.004021 0.005384 0.004125 0.001851 0.002550 0.005176 0.003143 0.002139 0.002934 0.005249 0.003713
Endosome 0.002188 0.002852 0.000477 0.001447 0.001536 0.002595 0.000427 0.002125 0.001652 0.002662 0.000445 0.001732
Golgi 0.000393 0.000888 0.000062 0.001024 0.000691 0.000984 0.000636 0.002872 0.000638 0.000959 0.000434 0.001800
Lipid Particles 0.003281 0.002215 0.000284 0.000337 0.002137 0.001367 0.000192 0.000497 0.002340 0.001588 0.000224 0.000404
Mitochondria 0.005957 0.004216 0.000890 0.003345 0.002226 0.003415 0.001161 0.002740 0.002889 0.003624 0.001066 0.003091
Mitotic Spindle 0.004084 0.006696 0.001039 0.021559 0.001333 0.009910 0.003479 0.058938 0.001822 0.009071 0.002618 0.037260
None 0.005027 0.007378 0.003501 0.006651 0.009604 0.008011 0.003655 0.008692 0.008790 0.007846 0.003600 0.007508
Nuclear Periphery 0.001011 0.001105 0.001054 0.001490 0.000687 0.001330 0.000962 0.000856 0.000745 0.001271 0.000995 0.001224
Nuclear Periphery Foci 0.000959 0.001171 0.000414 0.002074 0.000841 0.000672 0.000389 0.001187 0.000862 0.000802 0.000397 0.001702
Nucleolus 0.001418 0.002332 0.000326 0.000988 0.002207 0.002902 0.000314 0.001553 0.002066 0.002753 0.000318 0.001225
Nucleus 0.740752* 0.754960* 0.916585* 0.865568* 0.669674* 0.760369* 0.936891* 0.795996* 0.682312* 0.758957* 0.929727* 0.836344*
Peroxisomes 0.000887 0.001911 0.000081 0.000141 0.001401 0.000824 0.000434 0.001905 0.001310 0.001108 0.000310 0.000882
Vacuole 0.012322 0.012756 0.008978 0.008668 0.008536 0.011513 0.006192 0.010948 0.009210 0.011838 0.007175 0.009626
Vacuole Periphery 0.000566 0.001307 0.000084 0.000549 0.000570 0.000521 0.000073 0.000336 0.000570 0.000727 0.000077 0.000460

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.31 -6.40 -12.31 -0.35 -3.08 -1.87 -6.73 -11.27 -5.99 -4.63 -2.08 -9.00 -16.57 -3.67 -5.47
Bud Neck -3.11 -7.38 -16.68 -15.53 -15.04 -5.52 -8.29 -16.21 -12.79 -13.80 -6.17 -11.14 -23.19 -18.79 -20.06
Bud Site -1.77 -2.11 -11.41 -7.73 -7.65 -4.10 -2.86 -12.69 -7.51 -12.22 -4.43 -3.12 -17.00 -9.12 -14.90
Cell Periphery 1.04 1.35 -3.47 -4.69 -6.78 -0.98 3.67 -7.36 -4.17 -11.36 -0.55 3.17 -8.70 -5.80 -13.05
Cytoplasm -0.10 9.08 8.65 11.88 -1.74 10.32 30.76 23.65 14.18 -9.09 9.67 31.08 26.93 19.67 -8.98
Cytoplasmic Foci 3.93 6.60 6.27 5.98 -4.63 5.46 13.45 9.59 4.53 -5.17 6.57 14.61 12.56 8.17 -6.48
Eisosomes 3.81 5.23 4.64 0.79 -2.10 4.10 6.73 -2.59 -7.27 -10.93 5.12 7.88 1.88 -4.75 -9.56
Endoplasmic Reticulum -0.41 -1.93 -0.84 -1.31 3.83 -2.92 -9.82 -11.95 -7.48 2.51 -2.32 -9.61 -7.69 -7.03 4.07
Endosome -0.60 4.12 1.98 2.38 -3.27 -2.53 6.46 -2.62 0.69 -7.79 -2.74 7.44 -0.47 2.39 -7.47
Golgi -1.23 1.64 -1.06 0.43 -2.81 -0.58 0.64 -1.08 -0.60 -1.52 -0.80 1.08 -0.94 -0.08 -2.09
Lipid Particles 1.31 4.45 3.71 2.34 -4.74 1.67 4.82 2.63 1.16 -9.86 1.84 5.99 3.97 2.64 -10.06
Mitochondria 0.42 1.26 0.72 0.63 -4.92 -1.13 1.50 -0.38 1.07 -3.99 -0.63 1.98 0.05 1.03 -5.94
Mitotic Spindle -0.53 0.60 -8.56 -9.22 -11.92 -4.14 -1.22 -15.12 -13.30 -14.87 -4.02 -0.54 -18.72 -15.67 -18.76
None -1.42 2.14 -4.35 -1.63 -9.14 1.01 5.49 -1.21 -2.39 -10.67 0.78 5.80 -1.41 -2.41 -13.72
Nuclear Periphery -0.24 -1.18 -6.20 -9.55 -9.28 -3.58 -7.42 -14.21 -5.99 -10.21 -3.65 -7.17 -17.45 -9.08 -13.82
Nuclear Periphery Foci -0.08 1.46 -6.97 -7.05 -7.63 1.06 1.47 -8.10 -8.22 -8.28 0.61 2.11 -10.58 -10.63 -10.90
Nucleolus -1.65 5.18 -10.28 -8.20 -11.32 -1.20 3.42 -9.38 -8.62 -10.40 -1.53 4.30 -13.21 -11.74 -15.16
Nucleus -0.84 -9.06 -1.44 -0.71 16.51 -7.59 -28.99 0.23 7.11 25.22 -7.35 -29.52 -4.16 3.77 30.53
Peroxisomes -1.32 4.42 2.33 2.00 -5.22 0.77 1.77 -0.14 -1.88 -3.86 0.23 2.16 0.83 1.20 -4.36
Vacuole 0.07 -1.28 -4.32 -5.01 -3.06 -2.42 -1.70 -9.97 -8.14 -8.87 -2.38 -2.95 -12.08 -9.49 -9.29
Vacuole Periphery -0.84 1.26 0.62 1.24 -1.91 0.19 2.33 1.42 2.74 -4.31 -0.53 2.60 1.36 1.94 -3.04