Standard name
Human Ortholog
Description Serine-rich protein that contains a bHLH DNA binding motif; binds E-boxes of glycolytic genes and contributes to their activation; may function as a transcriptional activator in Ty1-mediated gene expression; bHLH stands for basic-helix-loop-helix
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.11 0.28 0.83 0.91 0.93 0.9 0.89 0.89 0.76 0.82 0.75 0.73 0.56 0.95 0.99 – 0.82 0.79 0.06 0.18 0.49 0.19 0.28 0.32
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0.19 0 0 – 0 0 0 0 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.08 0.15 0 0.06 0.08 0.11 0.14 0.1 0.41 0.24 0.43 0.38 0 0 0 – 0 0.08 0 0.05 0.12 0.07 0.08 0.09
Nucleus 0.63 0.41 0 0 0 0 0 0 0 0 0 0 0.1 0 0 – 0 0 0.58 0.38 0.06 0.38 0.16 0.12
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0.06 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0.08 0.11 0.09 0.08 0.13 0.15
Vac/Vac Membrane 0.25 0.25 0.1 0 0 0 0 0 0 0 0 0 0.19 0.05 0 – 0.17 0.09 0.09 0.15 0.09 0.13 0.14 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 4 6 3 5 3
Bud 0 0 0 3 1 2 3 1 7 4 15 11 0 0 0 – 0 1 9 10 8 8 15 14
Bud Neck 1 0 4 3 6 10 17 13 9 12 6 6 0 0 0 – 3 0 1 3 2 1 3 4
Bud Site 0 0 1 0 0 0 0 0 2 0 3 4 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 1 1 1 1 4 3 2 2 0 1 1 5 – 1 0 0 0 2 0 0 0
Cytoplasm 31 103 368 407 387 557 621 560 513 497 492 429 123 364 420 – 92 52 21 97 270 61 93 117
Endoplasmic Reticulum 3 2 4 0 0 0 0 0 0 0 0 0 1 0 0 – 3 6 0 0 5 0 1 3
Endosome 8 8 12 8 1 2 1 5 0 4 0 2 41 14 0 – 4 0 14 13 10 9 14 16
Golgi 0 1 0 0 1 0 0 1 0 0 0 0 0 0 0 – 0 0 1 1 2 1 1 1
Mitochondria 24 57 4 28 35 71 97 65 275 148 280 222 6 1 0 – 5 5 5 25 68 21 27 33
Nucleus 178 150 16 2 3 1 3 6 2 1 6 4 22 1 1 – 2 2 217 200 32 123 53 43
Nuclear Periphery 0 0 0 0 0 0 1 0 1 1 0 2 0 0 0 – 0 0 2 1 1 2 2 1
Nucleolus 0 0 0 0 5 0 1 2 0 1 1 1 2 0 1 – 0 0 22 10 4 9 5 4
Peroxisomes 0 0 0 0 0 2 0 2 0 1 0 0 7 0 3 – 1 0 0 1 0 3 6 1
SpindlePole 3 4 11 4 1 9 22 14 9 22 8 16 3 0 2 – 2 1 30 58 47 25 42 54
Vac/Vac Membrane 70 91 45 13 3 11 13 9 7 8 6 2 42 21 3 – 19 6 35 80 50 42 47 47
Unique Cell Count 284 369 446 447 416 619 700 627 671 609 653 585 221 384 425 112 66 375 528 550 324 334 366
Labelled Cell Count 318 416 465 469 444 666 780 682 828 701 819 699 248 402 435 132 73 375 528 550 324 334 366


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 4.2 5.1 4.4 4.3 3.9 3.9 4.3 3.2 3.7 3.3 3.4 5.2 4.9 5.1 4.3 5.8 5.1 6.3 6.2 6.4
Std Deviation (1e-4) 0.9 0.6 1.4 1.2 1.8 0.8 1.4 1.2 0.8 1.0 0.7 1.1 1.5 1.2 6.0 1.9 2.9 1.9 3.1 2.1 2.5
Intensity Change (Log2) – – – -0.19 -0.24 -0.39 -0.39 -0.25 -0.68 -0.46 -0.64 -0.58 0.04 -0.04 0.0 -0.23 0.2 -0.01 0.32 0.28 0.33


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 0
Bud Neck – – – – – – – – – – – – – 0 0
Bud Site – – – – – – – – – – – – – 0 0
Cell Periphery – – – – – – – – – – – – – 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 0
Endosome – – – – – – – – – – – – – 0 0
Golgi – – – – – – – – – – – – – 0 0
Mitochondria – – – – – – – – – – – – – 0 0
Nuclear Periphery – – – – – – – – – – – – – 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 0
Peroxisomes – – – – – – – – – – – – – 0 0
SpindlePole – – – – – – – – – – – – – 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 0
Cytoplasm – – – – – – – – – – – – – 0 0
Nucleus – – – – – – – – – – – – – 0 0
Vacuole – – – – – – – – – – – – – 2.0 -0.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.0967 3.7791 3.823 3.0327 3.3078 3.3773 4.0143 4.8806 4.3787 3.7585 4.0793 4.7175 1.0482 1.5988 1.4129 1.5944 0.9036 1.6995
Actin 0.0464 0.0003 0.0242 0.0089 0.0007 0.004 0.0016 0.0002 0.0009 0.0004 0.0003 0.0004 0.0105 0.0006 0.0004 0.0003 0.0006 0.0017
Bud 0.0005 0.0002 0.0004 0.0004 0.0144 0.0012 0.0003 0.0001 0.0103 0.0057 0 0.0035 0.0009 0.0023 0.0003 0.0002 0.0003 0.0002
Bud Neck 0.0011 0.0004 0.0032 0.0078 0.0003 0.0027 0.0023 0.0002 0.0008 0.0014 0.0002 0.0016 0.0095 0.0002 0.0002 0.0001 0.0005 0.0017
Bud Periphery 0.0008 0.0001 0.0005 0.0005 0.0261 0.0014 0.0005 0.0001 0.0046 0.0067 0 0.0011 0.002 0.0014 0.0003 0.0002 0.0004 0.0015
Bud Site 0.0029 0.0015 0.0031 0.0023 0.007 0.001 0.0017 0.0012 0.0045 0.0005 0 0.0001 0.0069 0.0038 0.0005 0.0001 0.0001 0.0001
Cell Periphery 0.0003 0.0001 0.0002 0.0002 0.0005 0.0001 0.0002 0.0001 0.0003 0.0001 0 0.0002 0.0006 0.0001 0.0001 0 0 0.0001
Cytoplasm 0.1728 0.1866 0.1595 0.1801 0.1875 0.2181 0.1371 0.2054 0.1722 0.2819 0.1105 0.1903 0.1624 0.1473 0.1201 0.1179 0.1541 0.2013
Cytoplasmic Foci 0.0544 0.0293 0.0083 0.0276 0.0514 0.0085 0.0099 0.0054 0.0154 0.0089 0.0127 0.0172 0.0092 0.0087 0.0117 0.0111 0.0072 0.007
Eisosomes 0.0006 0.0001 0.0004 0.0003 0 0.0001 0.0002 0.0004 0.0001 0 0 0 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0069 0.0021 0.0043 0.0028 0.002 0.0021 0.0032 0.0027 0.0024 0.0017 0.0012 0.0019 0.005 0.0021 0.0025 0.0031 0.0066 0.002
Endosome 0.0216 0.0024 0.0084 0.0448 0.0112 0.0053 0.0058 0.0023 0.002 0.002 0.0018 0.0024 0.0102 0.0022 0.0048 0.0036 0.0046 0.0036
Golgi 0.0054 0.0003 0.0067 0.0162 0.0048 0.0031 0.0005 0.0001 0.0002 0.0001 0.0001 0.0001 0.0011 0.0001 0.0004 0.0002 0.0001 0.0002
Lipid Particles 0.0132 0.0009 0.0101 0.0077 0.0174 0.0036 0.0007 0.0002 0.0008 0.0001 0.0005 0.0008 0.0026 0.0004 0.0007 0.0005 0.0004 0.0004
Mitochondria 0.0139 0.0003 0.0153 0.0047 0.004 0.0028 0.0017 0.0003 0.0015 0.0007 0.0003 0.0003 0.0018 0.0009 0.0007 0.0011 0.001 0.006
None 0.4781 0.6493 0.5892 0.5491 0.5895 0.5803 0.7217 0.6744 0.6389 0.6592 0.8511 0.642 0.565 0.7181 0.7337 0.8419 0.7741 0.6205
Nuclear Periphery 0.0241 0.003 0.0132 0.0083 0.0023 0.0038 0.0081 0.0019 0.0041 0.0022 0.002 0.0038 0.0081 0.0046 0.0057 0.0026 0.0127 0.0064
Nucleolus 0.0016 0.0014 0.0017 0.0035 0.0005 0.003 0.003 0.0012 0.0033 0.0014 0.0003 0.0011 0.0038 0.0019 0.0012 0.0004 0.001 0.002
Nucleus 0.0922 0.0968 0.1092 0.0848 0.0113 0.1344 0.0665 0.0835 0.1063 0.0149 0.0073 0.1044 0.1643 0.0841 0.1031 0.012 0.0208 0.1102
Peroxisomes 0.0214 0.0028 0.0083 0.0157 0.0521 0.0013 0.0003 0.0001 0.0004 0.0004 0.0001 0.0002 0.0014 0.0003 0.0014 0.0007 0.0002 0.0005
Punctate Nuclear 0.0378 0.0202 0.0314 0.0265 0.0054 0.0205 0.0316 0.0189 0.0291 0.0091 0.011 0.0272 0.0305 0.0193 0.0109 0.0022 0.0136 0.0332
Vacuole 0.0028 0.0018 0.0017 0.0068 0.0099 0.0023 0.0024 0.0011 0.0017 0.002 0.0006 0.0012 0.0033 0.0012 0.0012 0.0015 0.0011 0.0008
Vacuole Periphery 0.0012 0.0001 0.0009 0.001 0.0018 0.0005 0.0005 0.0001 0.0003 0.0004 0.0001 0.0002 0.0006 0.0002 0.0002 0.0004 0.0006 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 27.0813 51.796 29.2439 26.8197 32.6624 50.3559 48.1015 47.2572 35.2971 41.787
Translational Efficiency 2.4468 1.5628 1.8598 2.3169 2.0786 1.9053 1.9129 2.0047 2.5148 1.9788

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1252 292 2026 1977 2123 1762 2757 1922 3375 2054 4783 3899

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 681.42 641.01 774.01 824.57 667.94 783.25 824.95 847.84 672.94 763.03 803.37 836.04
Standard Deviation 79.88 131.14 95.99 116.39 86.59 102.09 105.67 115.74 84.41 117.70 104.75 116.66
Intensity Change Log 2 — -0.088197 0.183808 0.275098 — 0.229754 0.304588 0.344074 — 0.077937 0.244859 0.309653

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000172 0.000730 0.000300 0.000674 0.000116 0.000262 0.000280 0.000585 0.000137 0.000328 0.000288 0.000630
Bud Neck 0.003104 0.020791 0.023869 0.013715 0.002998 0.016947 0.008913 0.017880 0.003038 0.017494 0.015248 0.015768
Bud Site 0.000629 0.007544 0.012033 0.022320 0.000568 0.006093 0.003134 0.026884 0.000590 0.006299 0.006903 0.024570
Cell Periphery 0.000171 0.000313 0.000163 0.000222 0.000091 0.000308 0.000089 0.000160 0.000121 0.000309 0.000121 0.000191
Cytoplasm 0.178241* 0.279728* 0.150384* 0.223079* 0.199978* 0.237441* 0.091526 0.258466* 0.191914* 0.243452* 0.116458* 0.240523*
Cytoplasmic Foci 0.071064 0.158154 0.000953 0.007315 0.026760 0.071296 0.000853 0.006320 0.043196 0.083644 0.000895 0.006824
Eisosomes 0.000073 0.000129 0.000017 0.000034 0.000029 0.000082 0.000015 0.000026 0.000045 0.000088 0.000015 0.000030
Endoplasmic Reticulum 0.002444 0.004003 0.003228 0.004554 0.001275 0.001617 0.002912 0.004506 0.001708 0.001956 0.003046 0.004531
Endosome 0.002428 0.022838 0.000756 0.006378 0.000907 0.009812 0.000509 0.004246 0.001471 0.011664 0.000614 0.005327
Golgi 0.000918 0.003487 0.000062 0.002141 0.000238 0.002609 0.000048 0.002144 0.000490 0.002734 0.000054 0.002142
Lipid Particles 0.018704 0.019268 0.000314 0.001110 0.004474 0.017071 0.000321 0.000874 0.009753 0.017384 0.000318 0.000994
Mitochondria 0.006738 0.016239 0.002060 0.004610 0.003642 0.017632 0.001553 0.003694 0.004790 0.017434 0.001768 0.004159
Mitotic Spindle 0.002844 0.019433 0.005250 0.048948 0.001033 0.005425 0.004507 0.034476 0.001705 0.007416 0.004822 0.041814
None 0.019574 0.038959 0.006321 0.013569 0.010381 0.020770 0.006637 0.010272 0.013791 0.023355 0.006503 0.011944
Nuclear Periphery 0.003546 0.002790 0.003531 0.003749 0.002221 0.003409 0.002324 0.003970 0.002713 0.003321 0.002835 0.003858
Nuclear Periphery Foci 0.004027 0.003482 0.000466 0.001126 0.000971 0.001016 0.000310 0.001198 0.002105 0.001367 0.000376 0.001161
Nucleolus 0.015390 0.017020 0.002032 0.002715 0.007744 0.012860 0.002075 0.002530 0.010580 0.013452 0.002057 0.002624
Nucleus 0.658615* 0.329755* 0.777205* 0.603848* 0.731814* 0.544854* 0.868909* 0.586727* 0.704660* 0.514275* 0.830064* 0.595408*
Peroxisomes 0.006310 0.023611 0.000095 0.000543 0.001595 0.008417 0.000101 0.001120 0.003344 0.010577 0.000098 0.000827
Vacuole 0.004424 0.028589 0.009885 0.037040 0.002998 0.019785 0.004835 0.028197 0.003527 0.021037 0.006974 0.032681
Vacuole Periphery 0.000583 0.003135 0.001077 0.002308 0.000166 0.002294 0.000150 0.005724 0.000321 0.002413 0.000542 0.003992

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.78 -4.57 -6.56 0.61 -4.47 -1.73 -5.83 -10.80 -6.24 -7.09 -2.91 -7.24 -11.35 -5.47 -7.44
Bud Neck -5.35 -12.34 -12.08 1.89 5.31 -8.74 -12.68 -14.97 -1.73 -5.18 -10.00 -17.20 -19.34 0.17 0.56
Bud Site -5.43 -7.12 -8.17 -1.87 -0.88 -6.25 -5.00 -8.63 -4.66 -6.55 -7.38 -8.65 -11.86 -5.14 -5.67
Cell Periphery -3.57 1.99 2.27 4.47 -0.17 -8.75 1.50 -4.69 7.30 -6.07 -8.42 1.97 -1.77 7.84 -3.56
Cytoplasm -5.77 6.98 0.82 6.24 -6.85 -5.13 22.26 2.56 7.35 -18.31 -7.88 21.15 2.94 10.34 -18.53
Cytoplasmic Foci -6.80 19.11 18.22 14.23 -7.18 -13.36 16.88 14.59 24.06 -5.85 -10.79 24.73 22.79 27.38 -9.24
Eisosomes -5.17 15.82 8.09 8.57 -14.79 -13.93 5.41 -5.52 10.77 -13.84 -11.31 14.87 1.29 13.22 -20.41
Endoplasmic Reticulum -2.53 -7.33 -15.47 -4.64 -7.92 -1.84 -14.70 -19.07 -18.12 -10.01 -1.35 -15.89 -25.40 -22.39 -12.95
Endosome -5.36 7.46 -1.07 5.20 -5.87 -10.11 2.73 -4.52 8.08 -6.03 -10.90 7.26 -4.34 9.33 -8.36
Golgi -3.49 4.57 1.06 4.00 -3.75 -4.02 3.74 -1.71 2.86 -2.46 -4.22 5.64 -1.04 3.69 -4.14
Lipid Particles 0.34 12.43 11.96 6.89 -8.24 -9.12 9.61 8.18 12.75 -9.86 -5.18 15.14 14.08 14.27 -12.45
Mitochondria -2.72 5.56 4.25 3.96 -2.42 -7.98 2.80 1.00 8.98 -4.27 -8.10 5.49 3.27 9.76 -4.64
Mitotic Spindle -2.88 -1.87 -9.93 -2.72 -9.16 -4.06 -5.35 -11.27 -9.15 -8.68 -4.33 -5.32 -15.15 -11.31 -12.59
None -2.71 10.48 7.00 4.31 -4.46 -5.34 6.75 2.38 6.54 -5.50 -4.79 12.26 6.49 7.67 -6.72
Nuclear Periphery 1.17 -3.03 -6.34 -5.04 -3.73 -2.61 -6.41 -15.16 -6.80 -9.68 -1.39 -6.82 -15.81 -7.69 -9.70
Nuclear Periphery Foci 0.77 5.53 4.09 3.15 -2.93 0.59 2.56 -2.03 -2.70 -5.79 3.11 5.96 2.31 -1.33 -5.71
Nucleolus -0.78 10.32 10.72 5.20 0.31 -3.02 6.01 5.60 9.05 -0.20 -1.56 11.34 11.46 11.33 0.22
Nucleus 15.16 -14.83 -1.26 -16.45 16.10 18.72 -20.66 9.27 -9.75 29.22 20.45 -24.24 6.58 -15.57 32.79
Peroxisomes -3.84 7.11 6.91 5.61 -5.26 -6.67 4.13 2.83 8.48 -1.76 -6.31 8.15 7.32 10.28 -2.64
Vacuole -6.97 -8.14 -20.38 -8.08 -16.69 -10.66 -7.03 -20.00 -13.54 -17.88 -12.42 -10.73 -28.60 -17.88 -24.68
Vacuole Periphery -3.05 -0.41 -1.66 2.62 -0.40 -5.67 1.06 -2.75 0.49 -2.78 -6.05 -0.46 -3.29 2.10 -2.80
Standard name
Human Ortholog
Description Serine-rich protein that contains a bHLH DNA binding motif; binds E-boxes of glycolytic genes and contributes to their activation; may function as a transcriptional activator in Ty1-mediated gene expression; bHLH stands for basic-helix-loop-helix
Localization
Cell Percentages nucleus (83%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Tye7

Tye7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tye7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available