Standard name
Description Kynurenine aminotransferase; catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.05 0.06 0.14 0.09 0.06 0 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0 0 0.06 0 0 0.05
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.06 0 0 0 0 0 0 0 0 0 0.05 0.05 0.05 0.06 0.06 0 0 0 0 0 0 0
Cytoplasm 0.65 0.68 0.45 0.26 0.31 0.14 0.11 0.08 0.09 0.07 0.08 0.07 0.74 0.74 0.8 0.77 0.79 0.74 0.35 0.36 0.18 0.35 0.32 0.28
Endoplasmic Reticulum 0 0.06 0.12 0.05 0.08 0 0 0.05 0.1 0.05 0 0 0 0 0 0 0 0 0.06 0 0.06 0 0 0
Endosome 0.1 0.05 0.09 0.17 0.09 0.09 0.05 0.11 0 0 0 0 0.13 0.1 0.07 0 0 0 0.2 0.17 0.2 0.16 0.16 0.15
Golgi 0 0 0.15 0.15 0.23 0.09 0 0.1 0.1 0 0 0 0 0 0 0.06 0.06 0 0.05 0.05 0.13 0 0 0
Mitochondria 0.13 0.16 0.11 0.46 0.35 0.52 0.61 0.43 0.56 0.66 0.73 0.65 0 0 0 0 0 0 0.14 0.19 0.22 0.17 0.17 0.16
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0.05 0.06 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0.05 0.09 0.06 0.13 0.26 0.22 0.3 0.2 0.22 0.19 0.23 0.12 0.12 0.09 0.14 0.11 0.23 0 0.09 0 0.13 0.09 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 8 8 40 17 15 10 20 16 10 17 7 16 10 23 14 7 11 4 7 6 25 6 16 20
Bud 2 1 9 6 5 14 9 8 7 21 14 23 1 0 1 2 2 1 2 2 6 7 17 20
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 3 1 1 8 11
Bud Site 0 0 0 0 0 0 2 1 2 2 5 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 2 18 6 5 10 5 14 11 9 8 18 7 24 22 18 21 11 0 1 7 1 1 2
Cytoplasm 107 91 133 48 82 52 63 50 41 48 39 43 109 357 355 241 290 208 70 63 77 84 132 107
Endoplasmic Reticulum 7 8 35 9 22 16 25 30 47 33 19 27 4 20 16 9 7 11 12 3 25 4 10 15
Endosome 17 7 27 32 25 34 27 68 20 30 5 27 19 47 32 4 6 4 41 29 81 37 63 57
Golgi 5 3 44 29 62 33 19 66 48 30 16 27 1 4 3 19 22 10 9 8 52 9 17 16
Mitochondria 21 21 33 86 93 200 347 277 271 480 354 428 1 1 2 3 8 5 27 32 94 41 70 61
Nucleus 0 0 0 0 3 0 7 3 3 4 0 4 0 0 1 0 3 3 0 2 0 2 4 4
Nuclear Periphery 0 0 0 0 0 3 9 15 9 5 5 10 0 0 0 0 0 0 4 0 4 1 3 4
Nucleolus 0 0 1 1 2 10 28 36 15 36 8 24 0 0 0 0 0 1 0 0 0 0 2 1
Peroxisomes 0 0 1 1 0 0 0 1 0 0 0 0 0 0 3 0 0 0 0 0 1 0 1 0
SpindlePole 1 1 2 0 0 0 4 9 2 1 2 4 0 0 0 0 1 1 5 2 9 5 12 11
Vac/Vac Membrane 10 6 27 12 35 101 125 194 94 160 93 149 18 60 40 43 40 66 8 15 13 29 38 43
Unique Cell Count 164 133 295 188 264 383 565 644 481 725 484 655 148 482 441 314 368 282 203 176 419 239 411 390
Labelled Cell Count 180 148 370 247 349 483 690 788 580 876 575 801 170 536 489 346 411 325 203 176 419 239 411 390


Cytoplasm, Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.4 7.9 10.0 9.1 10.5 10.0 9.3 9.6 8.9 8.5 8.1 8.2 9.7 9.8 9.2 17.6 17.0 17.9 10.4 10.3 10.7
Std Deviation (1e-4) 2.1 2.0 2.2 2.1 2.1 2.3 2.1 2.1 2.1 2.0 2.2 2.1 1.8 1.8 1.9 4.7 4.7 5.5 2.0 2.1 2.1
Intensity Change (Log2) – – – -0.14 0.07 -0.0 -0.11 -0.06 -0.16 -0.24 -0.3 -0.29 -0.04 -0.02 -0.12 0.82 0.77 0.84 0.06 0.05 0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.5 -3.1 -5.4 -5.5 -6.7 -6.3 -7.1 -6.9 -6.7 -2.1 -4.4 -5.3 -5.2 -5.1 -5.5
Bud 0.1 -0.9 0.4 -1.4 -1.9 -1.5 -0.1 -0.1 0.4 0 0 0 -2.2 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.4 -2.5 -2.3 -4.5 -3.1 -2.7 -4.4 -3.4 -2.5 -0.6 -0.7 -0.7 -0.2 -0.2 -1.2
Cytoplasm -4.3 -3.4 -9.1 -11.3 -13.4 -11.9 -14.6 -12.1 -14.1 5.7 8.1 10.0 8.0 9.0 7.0
Endoplasmic Reticulum -2.6 -1.4 -3.8 -4.1 -4.0 -0.9 -4.2 -4.2 -4.5 -3.2 -4.1 -4.3 -4.3 -5.2 -3.5
Endosome 2.6 0.1 -0.1 -2.5 0.7 -2.8 -3.2 -5.5 -3.1 1.2 0.3 -0.9 -4.4 -4.4 -4.1
Golgi 0.2 2.6 -2.6 -6.2 -2.1 -2.1 -6.0 -5.9 -5.9 -4.7 -7.9 -7.7 -3.6 -3.8 -4.7
Mitochondria 8.6 6.8 11.2 14.1 9.6 12.5 15.9 16.8 15.5 -3.9 -7.3 -6.7 -5.4 -4.8 -4.6
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 3.6 3.8 2.6 3.6 0 3.0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.1 1.5 5.7 4.7 7.0 3.9 4.8 3.8 5.0 1.0 1.4 0 1.8 0.7 4.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.5966 11.7591 10.2194 10.1651 9.7146 9.9991 7.54 9.605 8.7786 7.7801 8.4739 8.6013 5.3889 7.4733 6.6146 5.6126 6.1513 6.4528
Actin 0.0391 0.0811 0.0885 0.0641 0.0462 0.036 0.0337 0.019 0.0454 0.0425 0.0226 0.0157 0.0439 0.04 0.0315 0.0384 0.0494 0.0266
Bud 0.007 0.0063 0.0119 0.0154 0.0118 0.0064 0.0053 0.0117 0.0105 0.0277 0.0134 0.004 0.0048 0.0061 0.0074 0.0159 0.0154 0.0071
Bud Neck 0.0024 0.0031 0.0056 0.0039 0.0023 0.0062 0.0032 0.0018 0.0035 0.0026 0.0016 0.007 0.0027 0.0018 0.0018 0.0018 0.0018 0.0072
Bud Periphery 0.007 0.0041 0.0123 0.0152 0.02 0.0094 0.0048 0.0051 0.0089 0.041 0.0149 0.0036 0.0052 0.0028 0.0062 0.0154 0.0128 0.0104
Bud Site 0.0142 0.0426 0.023 0.011 0.0049 0.0101 0.0129 0.0267 0.0132 0.0068 0.0034 0.002 0.0149 0.0295 0.007 0.0066 0.0076 0.0025
Cell Periphery 0.0011 0.0014 0.0071 0.0011 0.0009 0.0007 0.0006 0.0008 0.0007 0.0014 0.0014 0.0003 0.0008 0.0006 0.0005 0.0005 0.0003 0.0005
Cytoplasm 0.1834 0.3019 0.2284 0.335 0.1787 0.1248 0.237 0.3913 0.41 0.3728 0.3035 0.2789 0.2236 0.3675 0.3573 0.3035 0.2915 0.2831
Cytoplasmic Foci 0.1208 0.1419 0.1069 0.092 0.1224 0.113 0.1029 0.1209 0.0948 0.0854 0.1278 0.1104 0.1087 0.1482 0.1309 0.0934 0.1312 0.1199
Eisosomes 0.0003 0.0004 0.0007 0.0002 0.0003 0.0002 0.0002 0.0001 0.0002 0.0002 0.0004 0.0001 0.0002 0.0001 0.0001 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0475 0.0101 0.0177 0.0121 0.0402 0.0255 0.0282 0.0093 0.0142 0.0071 0.0141 0.0244 0.0269 0.0041 0.0102 0.0155 0.0048 0.014
Endosome 0.2237 0.1526 0.1365 0.1595 0.1791 0.2166 0.2756 0.1963 0.1504 0.184 0.2668 0.2726 0.2645 0.1818 0.1964 0.1866 0.1879 0.2453
Golgi 0.1478 0.1131 0.1364 0.1045 0.144 0.1968 0.0997 0.1153 0.1033 0.0665 0.0844 0.1245 0.1252 0.1555 0.1122 0.1328 0.1023 0.1392
Lipid Particles 0.0116 0.0026 0.0016 0.0012 0.0017 0.0025 0.0097 0.0013 0.0044 0.0023 0.0068 0.0062 0.0068 0.0013 0.0014 0.004 0.0045 0.0014
Mitochondria 0.1298 0.1124 0.1854 0.1461 0.1878 0.2053 0.0974 0.0587 0.1002 0.1332 0.097 0.1108 0.0681 0.0341 0.097 0.1435 0.1427 0.1037
None 0.0055 0.0013 0.0018 0.0012 0.0024 0.0049 0.004 0.0012 0.0017 0.0017 0.0015 0.0019 0.0113 0.0075 0.003 0.0011 0.0021 0.0014
Nuclear Periphery 0.0088 0.0028 0.0033 0.0024 0.0023 0.0055 0.0203 0.0034 0.0085 0.0026 0.0026 0.0054 0.0177 0.0008 0.0029 0.0034 0.0077 0.0067
Nucleolus 0.0004 0.0002 0.0002 0.0002 0.0001 0.0002 0.0013 0.0003 0.0004 0.0002 0.0002 0.0002 0.0013 0.0001 0.0001 0.0001 0.0005 0.0001
Nucleus 0.0026 0.002 0.0015 0.0028 0.0015 0.0021 0.0067 0.0026 0.0033 0.002 0.0016 0.0021 0.0109 0.001 0.0022 0.0019 0.0027 0.0021
Peroxisomes 0.0244 0.0093 0.0189 0.0084 0.0354 0.0147 0.0117 0.0121 0.0088 0.0066 0.0161 0.0074 0.0097 0.0095 0.011 0.0088 0.0198 0.0112
Punctate Nuclear 0.0015 0.0007 0.0005 0.0005 0.0009 0.0006 0.0045 0.0007 0.0011 0.0008 0.0062 0.0012 0.0127 0.0006 0.0007 0.0005 0.0032 0.0005
Vacuole 0.0129 0.0078 0.0076 0.0139 0.0117 0.0111 0.0273 0.0155 0.0112 0.0068 0.0077 0.0118 0.0291 0.0047 0.0141 0.0156 0.0061 0.0094
Vacuole Periphery 0.0082 0.0024 0.0043 0.0094 0.0055 0.0073 0.013 0.0061 0.0053 0.0056 0.0061 0.0095 0.0111 0.0025 0.0061 0.0103 0.0058 0.0077

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 58.2697 26.5989 36.1855 51.7561 36.9709 50.3898 44.0765 42.8582 52.9024 41.3054
Translational Efficiency 1.1553 1.4567 1.3017 1.3377 1.0915 1.6777 1.1914 1.3308 1.453 1.4664

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘