Standard name
Description Putative ortholog of human ETF-dH; found in a large supramolecular complex with other mitochondrial dehydrogenases; may have a role in oxidative stress response; ETF-dH is also known as electron transfer flavoprotein dehydrogenase

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0.06 0 0.05 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.06
Cell Periphery 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.06 0.07 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0.07 0.06 0 0.12 0.06 0.09 0.1 0 0 0 0 0 0.08 0.08 0.09 0.13 0.1 0 0.08 0.08 0.06 0 0
Golgi 0 0 0.28 0.16 0.2 0 0.05 0 0 0 0 0 0.16 0.21 0.26 0.09 0.13 0.07 0 0.06 0.06 0.07 0.1 0.14
Mitochondria 0.92 0.75 0.54 0.85 0.58 0.76 0.71 0.74 0.87 0.87 0.93 0.88 0.9 0.64 0.48 0.15 0.11 0.14 0.83 0.69 0.65 0.72 0.68 0.63
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0.06 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0.08 0.06 0.06 0.07 0.09 0.05 0.07 0 0 0 0.06 0 0 0 0 0 0 0 0
Nucleolus 0.06 0 0 0 0 0 0.09 0.05 0.06 0.05 0.07 0 0 0 0 0.11 0.13 0.1 0 0 0 0 0 0
Peroxisomes 0.07 0.12 0.14 0.09 0.11 0.05 0.07 0.06 0 0 0 0 0.05 0.21 0.24 0.13 0.19 0.15 0.07 0.05 0.11 0 0 0
SpindlePole 0 0 0.1 0 0 0 0 0.05 0 0 0 0 0 0.08 0.14 0.23 0.12 0.16 0 0 0 0 0 0
Vac/Vac Membrane 0.09 0.18 0.2 0.11 0.22 0.15 0.12 0.11 0.06 0.08 0 0.06 0.12 0.05 0.06 0.13 0.14 0.18 0 0 0 0.05 0.08 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 3 1 4 1 1 1 1 0 0 0 1 1 5 2 1 3 0 3 10 4 2 3 5
Bud 11 6 2 5 4 3 7 15 3 3 6 8 0 10 10 3 4 5 0 5 0 0 2 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 3 0 1 0 1 0 3 2 2 1 0 0 0 0 3 2 6
Cell Periphery 5 10 6 9 5 9 5 2 4 4 2 2 0 3 5 5 7 7 0 0 0 0 0 0
Cytoplasm 2 4 1 2 5 4 7 7 4 2 5 7 0 1 5 2 5 9 0 0 0 0 4 0
Endoplasmic Reticulum 3 1 0 0 0 3 0 1 1 1 0 2 0 0 0 1 3 4 0 0 0 0 0 1
Endosome 10 22 7 8 31 16 30 51 9 9 1 8 2 13 16 5 14 10 4 29 11 7 7 6
Golgi 2 10 34 36 51 12 16 14 2 4 0 9 9 32 52 5 14 7 13 23 9 7 19 29
Mitochondria 303 254 66 188 146 206 241 392 187 222 202 272 52 99 97 8 12 14 278 254 97 81 135 133
Nucleus 3 2 0 0 0 0 10 5 1 3 2 1 0 2 0 3 5 6 0 0 0 0 0 0
Nuclear Periphery 11 8 3 3 5 23 22 33 15 24 11 21 1 1 2 3 3 4 2 0 0 0 1 0
Nucleolus 19 9 4 6 5 12 29 26 13 12 15 13 0 4 1 6 14 10 0 1 0 0 0 0
Peroxisomes 24 42 17 21 27 14 24 32 5 4 0 6 3 33 48 7 21 15 23 16 15 3 5 7
SpindlePole 5 8 12 4 9 7 13 28 4 4 2 10 0 12 27 12 13 16 2 6 4 1 0 0
Vac/Vac Membrane 30 62 25 24 55 42 40 56 13 20 8 19 7 7 12 7 15 19 2 11 3 5 16 16
Unique Cell Count 328 338 123 222 251 272 341 528 214 254 218 308 58 154 200 53 109 103 337 366 150 113 199 210
Labelled Cell Count 429 444 178 311 344 353 445 666 263 314 255 379 75 222 277 71 135 132 337 366 150 113 199 210


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.9 9.0 9.4 9.1 10.1 8.3 8.5 8.1 7.2 7.4 6.6 7.3 9.4 9.8 9.5 10.2 11.3 10.8 8.6 10.0 10.8
Std Deviation (1e-4) 1.7 2.1 1.5 1.9 2.0 2.1 1.8 1.8 1.8 1.5 1.9 1.7 2.0 1.7 1.7 3.6 3.9 4.0 2.7 2.1 2.2
Intensity Change (Log2) -0.04 0.12 -0.17 -0.13 -0.21 -0.38 -0.34 -0.49 -0.36 0.01 0.07 0.02 0.12 0.27 0.21 -0.12 0.09 0.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 2.0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.4 0 0 0 0 0 0 0 0 0 0 0 0 0.5 0.6
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -0.9 2.0 0.1 1.1 1.4 -0.6 -1.0 0 0 0 0.9 0.8 0 1.9 1.1
Golgi -2.5 -1.6 -6.7 -7.0 -9.6 -7.6 -7.9 -8.2 -7.7 -1.8 -1.3 -0.3 -2.7 -2.8 -4.1
Mitochondria 6.3 0.8 4.4 3.4 4.5 6.9 7.2 8.4 7.9 4.7 1.8 -0.9 -4.8 -6.9 -6.3
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 2.2 1.7 1.7 1.8 2.5 1.2 1.8 0 0 0 0 0 0
Nucleolus 0 0 0 1.9 0.8 1.1 0.7 1.4 0 0 0 0 0 2.7 2.0
Peroxisomes -1.2 -0.9 -3.0 -2.3 -2.9 -4.1 -4.9 -5.6 -5.0 -1.7 1.6 2.2 -0.1 1.1 0.2
SpindlePole -3.4 -2.4 -3.1 -2.5 -1.9 -3.3 -3.7 -4.0 -2.8 0 -0.6 1.0 2.3 0.5 1.3
Vacuole -2.4 0.4 -1.2 -2.4 -2.9 -4.0 -3.5 -5.0 -4.4 -1.4 -4.1 -3.9 -1.1 -1.3 -0.4


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cir2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available