Standard name
Human Ortholog
Description Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.06 0 0.06 0.08 0.06 0.17 0.18 0.21 0.26 0.3 0.36 0.33 0.14 0.17 0.29 0.36 0.4 0.29 0 0 0 0.1 0.1 0.09
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0.11 0 0 0.12 0.15 0.19 0.05 0.06 0.09 0.08 0.05 0.09 0.08 0.11 0 0.13 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0 0
Nucleus 0.53 0.54 0.6 0.59 0.29 0.25 0.29 0.22 0.21 0.2 0.23 0.18 0.24 0.24 0.18 0.32 0.29 0.13 0.79 0.79 0.7 0.64 0.55 0.41
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.35 0 0 0 0 0 0 0 0.05 0 0 0 0.07 0.07 0.07 0 0 0 0.08 0 0.11 0.05 0.06 0.09
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.18 0.42 0.3 0.3 0.66 0.52 0.53 0.47 0.47 0.48 0.43 0.48 0.54 0.51 0.44 0.31 0.4 0.58 0.1 0.12 0.12 0.1 0.16 0.28
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 2 3 3 2 1 0 0 0 1 0 0 1 0 1 1 0 1 0
Bud 0 0 0 0 1 0 0 0 1 0 1 0 0 0 0 0 0 1 0 5 8 6 3 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 1 0 0 0 0 3 1 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 2 11 20 10 15 33 61 68 64 80 79 96 8 22 33 26 14 20 0 3 2 15 14 14
Endoplasmic Reticulum 0 0 0 0 0 1 1 4 1 1 0 2 1 0 1 4 1 2 0 1 0 1 3 2
Endosome 0 1 0 0 0 0 0 1 0 0 1 0 0 0 1 1 0 0 0 1 3 1 2 3
Golgi 0 4 37 4 8 23 52 63 11 17 20 25 3 12 9 8 0 9 0 0 0 0 0 2
Mitochondria 1 0 0 0 0 2 0 1 3 3 2 0 0 0 0 4 2 1 0 1 0 1 0 1
Nucleus 18 144 200 76 70 48 97 72 52 55 51 53 14 31 21 23 10 9 55 276 313 99 78 64
Nuclear Periphery 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 1 3 1
Nucleolus 12 11 9 3 5 4 7 8 11 5 3 8 4 9 8 0 1 3 5 14 48 7 8 14
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 1 1 2
Vac/Vac Membrane 6 112 99 39 158 99 180 155 113 130 96 143 32 67 51 22 14 40 6 40 54 15 22 43
Unique Cell Count 34 265 331 128 238 189 337 327 242 269 222 295 59 131 115 72 35 69 70 350 445 155 143 158
Labelled Cell Count 39 283 366 133 257 210 401 375 259 293 254 331 63 141 125 88 42 87 70 350 445 155 143 158


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 38.6 32.8 32.7 32.1 28.6 31.8 30.3 28.5 24.7 26.7 25.4 24.7 39.0 34.3 34.2 33.3 34.2 36.0 34.1 33.0 38.2
Std Deviation (1e-4) 11.0 8.4 8.0 6.9 7.4 7.6 6.6 6.9 6.4 7.3 7.1 7.4 12.7 11.5 12.5 11.9 15.8 17.3 13.0 14.8 15.7
Intensity Change (Log2) – – – -0.03 -0.19 -0.04 -0.11 -0.2 -0.41 -0.29 -0.36 -0.4 0.26 0.07 0.06 0.03 0.07 0.14 0.06 0.01 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.7 0.1 4.1 4.8 5.6 6.8 7.7 8.9 8.5 0 3.6 6.5 7.3 0 5.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi -2.7 -3.4 0.3 1.6 2.9 -2.8 -2.1 -0.8 -1.1 -1.4 -0.6 -1.0 0 0 0.4
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus -0.2 -7.3 -7.7 -8.2 -10.0 -9.3 -9.9 -8.7 -10.8 -5.2 -7.1 -7.8 -4.4 -3.6 -7.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 -0.5 0 -0.5 -0.2 1.2 -0.7 0 0 0 2.1 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.1 8.6 5.1 6.2 4.6 4.1 4.6 3.2 4.8 3.6 4.3 2.8 0.1 1.2 4.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 34.4128 40.5512 36.7601 36.6684 35.6708 36.6529 37.1501 46.1961 38.7445 38.6972 38.1478 40.3618 30.1117 36.3718 31.628 31.8768 24.7309 35.5109
Actin 0.0469 0.0001 0.0154 0 0.0417 0.008 0.002 0.0002 0.0016 0.0001 0.0212 0.0002 0.0271 0 0.0142 0.0002 0.0209 0.0001
Bud 0.0008 0.0001 0.0003 0 0.0001 0.0003 0.0004 0.0002 0.0002 0 0.0006 0.0001 0.0003 0 0.0001 0.0002 0.0004 0
Bud Neck 0.0004 0.0001 0.0002 0 0.0002 0.0015 0.0003 0.0001 0.0002 0.0001 0.0003 0.0004 0.0007 0 0.0001 0.0001 0.0005 0.0004
Bud Periphery 0.0011 0.0001 0.0003 0 0.0001 0.0005 0.0004 0.0001 0.0002 0 0.0011 0.0001 0.0003 0 0.0001 0.0002 0.0006 0.0001
Bud Site 0.001 0.0001 0.0022 0 0.0003 0.0003 0.0009 0.0001 0.0007 0 0.0004 0.0001 0.0028 0 0.0006 0.0002 0.0011 0
Cell Periphery 0.0007 0.0001 0.0001 0 0 0.0002 0.0003 0 0.0001 0 0.0002 0 0.0004 0 0.0001 0.0003 0.0004 0
Cytoplasm 0.0092 0.0011 0.0027 0.0008 0.0007 0.005 0.0204 0.0012 0.016 0.0007 0.0012 0.0018 0.0176 0.0005 0.0008 0.0018 0.0057 0.0003
Cytoplasmic Foci 0.0125 0.0008 0.0054 0 0.005 0.0014 0.0079 0 0.0054 0 0.0025 0.0002 0.0054 0 0.0165 0.006 0.0181 0
Eisosomes 0.0005 0 0.0001 0 0.0002 0.0001 0 0 0 0 0.0001 0 0.0004 0 0 0 0.0004 0
Endoplasmic Reticulum 0.0037 0 0.0005 0 0.0001 0.0006 0.0024 0 0.0016 0 0.0011 0.0005 0.0013 0 0.0001 0.0001 0.0019 0
Endosome 0.0142 0.0001 0.0056 0 0.0018 0.003 0.006 0 0.0145 0 0.0043 0.0035 0.0142 0 0.0017 0.0005 0.027 0
Golgi 0.0088 0 0.0041 0 0.0049 0.0044 0.0005 0 0.0035 0 0.0016 0.0001 0.0045 0 0.0018 0.0001 0.0187 0
Lipid Particles 0.0057 0.0002 0.0034 0 0.014 0.0031 0.0032 0 0.0073 0 0.0054 0.0002 0.0023 0 0.0026 0.0067 0.021 0
Mitochondria 0.008 0.0015 0.0022 0.0004 0.0028 0.012 0.0047 0.0012 0.0028 0.0008 0.0104 0.0021 0.006 0.0004 0.002 0.0029 0.0606 0.0003
None 0.0057 0.0002 0.0038 0 0.0003 0.0007 0.0133 0 0.0014 0 0.001 0.0002 0.015 0 0.0008 0.0011 0.0116 0
Nuclear Periphery 0.0262 0.0101 0.018 0.0191 0.028 0.0123 0.0333 0.0312 0.0261 0.0214 0.0538 0.0244 0.0118 0.0118 0.0077 0.0173 0.0195 0.0193
Nucleolus 0.0098 0.0123 0.0088 0.002 0.011 0.0297 0.0192 0.0064 0.0084 0.0021 0.0417 0.0161 0.0303 0.0057 0.024 0.0296 0.1031 0.0118
Nucleus 0.8199 0.9711 0.9158 0.9775 0.8683 0.91 0.8329 0.9583 0.8924 0.9734 0.8363 0.9455 0.8441 0.981 0.9204 0.9255 0.659 0.9665
Peroxisomes 0.0057 0.0001 0.0035 0 0.0194 0.0008 0.0003 0 0.0019 0 0.0013 0.0001 0.002 0 0.0026 0.0003 0.0097 0
Punctate Nuclear 0.0106 0.0003 0.006 0.0001 0.0005 0.001 0.0427 0.0002 0.0101 0.0001 0.0058 0.0014 0.0064 0.0001 0.0024 0.0017 0.0147 0.0001
Vacuole 0.0031 0.0007 0.0005 0.0001 0.0003 0.0024 0.006 0.0002 0.0035 0.0004 0.0018 0.0013 0.004 0.0001 0.0005 0.0022 0.0019 0.0003
Vacuole Periphery 0.0054 0.0011 0.0011 0.0002 0.0004 0.0028 0.0029 0.0006 0.002 0.0007 0.0081 0.0017 0.0032 0.0002 0.0007 0.0029 0.0033 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 171.174 113.7689 136.7627 158.9872 123.7554 104.3011 171.3971 168.0875 180.1412 136.6761
Translational Efficiency 1.3549 1.7675 1.6123 1.3515 1.3537 2.312 1.411 1.5069 1.3322 1.3819

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1518 916 2834 1963 1070 2317 134 1531 2588 3233 2968 3494

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1917.68 2611.87 2965.13 3239.95 2366.09 2473.82 3308.26 3392.90 2103.07 2512.93 2980.62 3306.97
Standard Deviation 450.44 518.66 557.31 694.71 405.55 489.96 478.36 671.37 485.57 502.13 558.55 688.78
Intensity Change Log 2 — 0.445721 0.628733 0.756610 — 0.064236 0.483568 0.520014 — 0.247563 0.550364 0.630748

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000945 0.001139 0.000557 0.000750 0.001406 0.000741 0.001873 0.000882 0.001136 0.000854 0.000616 0.000808
Bud Neck 0.024790 0.031375 0.026603 0.030060 0.030773 0.028213 0.027907 0.035111 0.027263 0.029109 0.026661 0.032273
Bud Site 0.001124 0.001726 0.000877 0.005499 0.001196 0.001310 0.002009 0.002830 0.001154 0.001428 0.000928 0.004330
Cell Periphery 0.000236 0.000232 0.000070 0.000296 0.000144 0.000193 0.000151 0.000212 0.000198 0.000204 0.000074 0.000260
Cytoplasm 0.009201 0.002332 0.002643 0.015070 0.002715 0.004380 0.000627 0.009486 0.006519 0.003800 0.002552 0.012623
Cytoplasmic Foci 0.000167 0.001153 0.000136 0.001559 0.000433 0.000336 0.001449 0.001712 0.000277 0.000568 0.000195 0.001626
Eisosomes 0.000020 0.000031 0.000011 0.000012 0.000023 0.000018 0.000090 0.000015 0.000021 0.000022 0.000015 0.000013
Endoplasmic Reticulum 0.002475 0.001511 0.001246 0.000901 0.002748 0.001917 0.000719 0.000897 0.002588 0.001802 0.001222 0.000899
Endosome 0.000216 0.000520 0.000161 0.001293 0.000315 0.000524 0.000080 0.001344 0.000257 0.000523 0.000157 0.001315
Golgi 0.000094 0.000488 0.000088 0.002333 0.000289 0.000358 0.000294 0.002345 0.000174 0.000395 0.000097 0.002339
Lipid Particles 0.000027 0.000221 0.000010 0.000122 0.000095 0.000183 0.000083 0.000098 0.000055 0.000194 0.000013 0.000112
Mitochondria 0.002644 0.003468 0.002706 0.004541 0.005532 0.002469 0.006267 0.005179 0.003838 0.002752 0.002867 0.004821
Mitotic Spindle 0.000172 0.005524 0.001449 0.021376 0.001711 0.003502 0.000943 0.010448 0.000808 0.004075 0.001426 0.016588
None 0.005317 0.001925 0.002260 0.006864 0.001338 0.002044 0.001333 0.004146 0.003672 0.002010 0.002218 0.005673
Nuclear Periphery 0.002844 0.002197 0.004015 0.009408 0.011523 0.002771 0.004466 0.012977 0.006432 0.002608 0.004035 0.010972
Nuclear Periphery Foci 0.000481 0.000549 0.000147 0.001013 0.002641 0.000564 0.000295 0.001396 0.001374 0.000560 0.000153 0.001181
Nucleolus 0.024712 0.057312 0.017904 0.011964 0.055935 0.041008 0.083055 0.014690 0.037621 0.045628 0.020845 0.013159
Nucleus 0.921601* 0.884842* 0.938434* 0.883612* 0.877327* 0.907489* 0.860670* 0.893812* 0.903296* 0.901073* 0.934923* 0.888082*
Peroxisomes 0.000175 0.000618 0.000153 0.000251 0.000585 0.000239 0.005255 0.000327 0.000344 0.000347 0.000384 0.000284
Vacuole 0.001315 0.001645 0.000318 0.002309 0.001824 0.000857 0.001847 0.001402 0.001525 0.001080 0.000387 0.001912
Vacuole Periphery 0.001444 0.001191 0.000213 0.000765 0.001447 0.000883 0.000587 0.000691 0.001445 0.000970 0.000230 0.000733

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.99 6.86 2.84 4.84 -3.91 12.26* -3.92 7.90 -6.07 7.51 6.97 11.89 7.20 -0.42 -5.39
Bud Neck -4.69 -5.46 -7.06 -1.52 -2.89 1.69 -1.62 -7.60 -9.80 -2.44 -2.17 -4.16 -10.47 -8.64 -6.84
Bud Site -1.90 0.39 -5.02 -3.05 -5.12 -0.37 -3.57 -4.43 -4.33 0.24 -1.87 0.13 -6.64 -5.50 -6.64
Cell Periphery 0.13 4.99 0.20 -0.03 -5.64 -0.66 -2.32 -3.52 -1.05 -0.33 0.03 5.73 -1.37 -0.78 -8.33
Cytoplasm 5.41 5.49 -2.02 -8.13 -8.65 -1.89 3.79 -7.52 -4.74 -10.72 2.93 5.05 -4.88 -8.07 -11.44
Cytoplasmic Foci -2.41 0.61 -3.30 0.64 -3.44 0.71 -1.94 -2.89 -3.24 1.32 -1.72 -0.04 -4.34 -2.45 -3.97
Eisosomes -5.13 3.74 2.57 9.10 -2.54 3.59 -9.17 3.38 -0.20 9.78 -0.60 2.74 3.87 6.42 1.37
Endoplasmic Reticulum 6.14 10.53 12.83 4.37 5.54 4.26 12.73 12.04 5.51 -2.75 5.74 14.65 17.57 6.67 5.46
Endosome -1.75 1.47 -2.15 -0.51 -2.47 -1.03 4.87 -1.22 -0.67 -1.87 -1.88 2.47 -2.23 -0.82 -2.82
Golgi -2.12 -3.25 -2.25 -1.26 -2.16 -0.23 -1.51 -1.43 -1.32 -1.19 -1.39 0.95 -2.57 -1.90 -2.66
Lipid Particles -1.68 1.71 -5.05 0.66 -5.73 -0.46 -0.74 -1.47 -0.38 -0.99 -1.17 4.16 -3.04 0.02 -4.10
Mitochondria -4.99 -5.59 -12.27 -6.45 -8.81 8.99 -1.74 -0.60 -16.91 1.56 5.96 2.17 -7.93 -17.22 -12.88
Mitotic Spindle -2.56 -4.40 -8.55 -4.89 -7.32 -1.40 -0.50 -5.75 -5.03 -4.27 -3.45 -2.53 -10.28 -7.60 -9.14
None 3.14 2.53 -0.93 -6.14 -5.40 -2.38 -1.39 -4.20 -3.21 -3.65 2.58 1.61 -2.51 -6.98 -5.27
Nuclear Periphery 0.83 -2.80 -5.44 -5.66 -4.22 5.45 3.95 -0.52 -7.30 -4.92 5.05 3.00 -3.73 -9.09 -7.24
Nuclear Periphery Foci 0.07 2.12 -1.43 -1.75 -5.42 3.31 3.87 1.88 -3.37 -5.09 3.00 4.39 1.13 -3.58 -7.22
Nucleolus -7.65 0.55 3.92 10.06 4.16 3.64 -1.74 10.75 9.64 5.04 -3.44 5.45 10.58 14.18 5.45
Nucleus 6.32 -0.24 7.99 0.30 8.90 -5.01 1.70 1.46 6.83 -1.07 1.12 -4.76 6.91 5.86 11.78
Peroxisomes -5.15 -1.88 -3.47 4.01 -1.87 5.02 -5.66 3.05 -3.18 5.94 -0.67 -1.87 0.81 1.57 2.61
Vacuole -0.66 3.93 -3.18 -1.65 -4.97 4.03 -1.95 -2.42 -4.56 0.20 1.96 4.90 -4.03 -4.91 -6.58
Vacuole Periphery 0.46 3.21 2.48 1.68 -2.64 2.05 3.53 3.65 2.31 0.55 1.64 4.98 3.80 2.94 -3.80
Standard name
Human Ortholog
Description Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages nucleus (82%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-5

Pre10

Pre10


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pre10-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available