Standard name
Description Putative protein of unknown function; functions together with HAM1 to mediate resistance to 5-FU; specifically reduces the incorporation of 5-FU into RNA, without affecting uptake or incorporation of uracil into RNA; proposed to be involved in the metabolism of purine and pyrimidine base analogues; deletion mutants are sensitive to HAP and AHA

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.99 0.98 0.95 0.91 0.84 0.79 0.66 0.75 0.72 0.69 0.97 0.99 0.99 0.98 0.99 0.94 0.97 0.98 0.97 0.96 0.93 0.97
Endoplasmic Reticulum 0 0 0 0 0 0 0.05 0.05 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.06 0.11 0.15 0.24 0.14 0.23 0.21 0 0 0 0 0 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 1 2 1 2 3 5 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 1 0 5 1 0 1 0 0 0 1 0 0 0 0 0 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1 1 4 2 4 10 8 14 6 7 5 16 3 2 2 2 2 2 0 0 0 0 0 0
Cytoplasm 94 176 291 259 337 336 337 366 158 222 198 311 210 372 408 227 304 266 114 174 276 265 339 401
Endoplasmic Reticulum 0 1 1 2 8 10 20 24 8 13 6 21 1 2 1 5 9 8 0 0 2 1 1 2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2
Golgi 0 0 1 0 0 1 0 2 1 1 0 0 0 0 0 1 0 0 0 0 1 0 1 0
Mitochondria 0 1 0 0 0 2 1 1 18 10 11 18 0 0 0 3 3 1 0 0 0 1 2 0
Nucleus 1 1 0 4 7 8 7 8 1 6 6 10 2 5 3 7 2 0 1 1 0 2 6 3
Nuclear Periphery 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 1 2 11 21 45 68 59 41 63 96 9 1 12 3 6 19 1 1 0 0 5 1
Unique Cell Count 96 177 293 264 353 369 401 466 241 296 276 451 217 377 414 232 306 282 118 178 286 277 367 416
Labelled Cell Count 97 180 298 269 367 389 420 485 258 303 292 478 225 382 426 250 326 297 118 178 286 277 367 416


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.8 10.6 14.2 13.6 14.9 13.2 13.4 12.8 11.8 12.5 12.1 12.4 15.8 16.1 16.2 14.7 19.0 19.6 14.8 14.9 15.6
Std Deviation (1e-4) 2.7 1.8 1.9 2.0 2.2 2.0 2.4 2.2 2.3 2.4 2.3 2.5 2.6 2.4 2.2 3.6 4.5 5.3 2.4 3.2 2.8
Intensity Change (Log2) -0.06 0.08 -0.1 -0.08 -0.15 -0.26 -0.18 -0.23 -0.19 0.16 0.18 0.19 0.05 0.42 0.47 0.06 0.07 0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 1.2 0.6 1.4 0 0.9 0 1.8 0 0 0 0 0 0
Cytoplasm -1.3 -3.0 -4.7 -6.8 -8.2 -10.6 -8.8 -9.5 -10.3 -2.2 -0.8 -0.9 -1.5 0 -3.4
Endoplasmic Reticulum 0 0 0 3.5 3.6 0 3.2 0 3.4 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 4.8 0 3.5 3.5 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 2.6 3.8 5.7 6.6 8.8 6.4 8.5 8.3 0 0 2.5 0 0 4.2


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Log1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available