Standard name | |
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Description | High-affinity S-adenosylmethionine permease; required for utilization of S-adenosylmethionine as a sulfur source; has similarity to S-methylmethionine permease Mmp1p |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 1 | 3 | 1 | 0 | 1 | 2 | 2 | – | 0 | 2 | 5 | 0 | 0 | 0 | 11 | 5 | 2 | 4 | 5 | 4 | 1 | 2 | 4 |
Bud | 0 | 0 | 2 | 0 | 1 | 3 | 5 | 7 | – | 12 | 12 | 17 | 0 | 0 | 0 | 0 | 3 | 0 | 4 | 5 | 6 | 1 | 0 | 2 |
Bud Neck | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 1 | 2 | 2 | 2 | 0 | 0 | 0 | 3 | 3 | 2 | 0 | 0 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | – | 2 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 4 | 2 | 2 | 2 | 3 | 6 | – | 6 | 7 | 16 | 8 | 6 | 5 | 37 | 12 | 6 | 1 | 1 | 3 | 1 | 0 | 1 |
Cytoplasm | 255 | 205 | 201 | 185 | 215 | 183 | 222 | 242 | – | 150 | 120 | 126 | 417 | 351 | 449 | 28 | 29 | 18 | 197 | 157 | 95 | 66 | 115 | 139 |
Endoplasmic Reticulum | 7 | 14 | 10 | 11 | 22 | 13 | 18 | 12 | – | 20 | 20 | 39 | 5 | 5 | 7 | 1 | 0 | 2 | 18 | 28 | 56 | 4 | 3 | 6 |
Endosome | 1 | 0 | 3 | 0 | 1 | 2 | 2 | 7 | – | 2 | 0 | 3 | 2 | 0 | 1 | 0 | 1 | 0 | 3 | 1 | 3 | 1 | 1 | 4 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 7 | 7 | 10 | 0 | 1 | 0 | 0 | 2 | 3 |
Mitochondria | 10 | 9 | 14 | 22 | 14 | 103 | 165 | 225 | – | 216 | 174 | 204 | 0 | 1 | 0 | 11 | 2 | 3 | 29 | 13 | 47 | 10 | 15 | 13 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 4 | – | 1 | 5 | 2 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 1 | 0 | 0 | 1 | 3 | 4 | – | 3 | 2 | 13 | 0 | 0 | 0 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 2 |
Nucleolus | 1 | 0 | 2 | 0 | 1 | 1 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | – | 3 | 0 | 6 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 4 | 4 | 1 | 1 | 3 |
Vac/Vac Membrane | 0 | 2 | 5 | 5 | 8 | 11 | 9 | 41 | – | 24 | 24 | 40 | 0 | 0 | 1 | 12 | 18 | 3 | 5 | 7 | 5 | 2 | 4 | 3 |
Unique Cell Count | 271 | 223 | 225 | 207 | 257 | 261 | 345 | 446 | 336 | 283 | 351 | 425 | 357 | 458 | 105 | 70 | 41 | 278 | 234 | 237 | 94 | 151 | 193 | |
Labelled Cell Count | 279 | 231 | 245 | 226 | 264 | 325 | 431 | 556 | 439 | 366 | 477 | 434 | 365 | 467 | 113 | 78 | 45 | 278 | 234 | 237 | 94 | 151 | 193 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 4.0 | 4.8 | 4.5 | 4.0 | 4.8 | 3.8 | 4.1 | 4.0 | – | 3.7 | 4.2 | 4.8 | 4.9 | 5.0 | 5.2 | 14.0 | 14.3 | 19.3 | 5.4 | 5.5 | 6.2 |
Std Deviation (1e-4) | 1.1 | 2.4 | 2.7 | 1.8 | 3.9 | 2.0 | 3.0 | 1.8 | – | 2.0 | 2.4 | 2.4 | 0.8 | 1.0 | 1.1 | 3.3 | 3.9 | 4.8 | 3.4 | 2.0 | 3.5 |
Intensity Change (Log2) | – | – | – | -0.18 | 0.09 | -0.24 | -0.14 | -0.18 | – | -0.28 | -0.12 | 0.08 | 0.13 | 0.15 | 0.19 | 1.63 | 1.66 | 2.09 | 0.27 | 0.28 | 0.46 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
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Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 3.5 | 6.4 | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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