Standard name
Description High-affinity S-adenosylmethionine permease; required for utilization of S-adenosylmethionine as a sulfur source; has similarity to S-methylmethionine permease Mmp1p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.07 0.05 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.35 0.17 0.15 0 0 0 0 0 0
Cytoplasm 0.94 0.92 0.89 0.89 0.84 0.7 0.64 0.54 0.45 0.42 0.36 0.98 0.98 0.98 0.27 0.41 0.44 0.71 0.67 0.4 0.71 0.76 0.72
Endoplasmic Reticulum 0 0.06 0 0.05 0.09 0.05 0.05 0 0.06 0.07 0.11 0 0 0 0 0 0.05 0.07 0.12 0.24 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.1 0.24 0 0 0 0 0 0
Mitochondria 0 0 0.06 0.11 0.05 0.39 0.48 0.5 0.64 0.61 0.58 0 0 0 0.1 0 0.07 0.11 0.06 0.2 0.11 0.1 0.07
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.09 0.07 0.08 0.11 0 0 0 0.11 0.26 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 3 1 0 1 2 2 0 2 5 0 0 0 11 5 2 4 5 4 1 2 4
Bud 0 0 2 0 1 3 5 7 12 12 17 0 0 0 0 3 0 4 5 6 1 0 2
Bud Neck 2 0 0 0 0 0 0 1 0 0 1 2 2 2 0 0 0 3 3 2 0 0 2
Bud Site 0 0 0 0 0 1 0 2 2 0 3 0 0 0 0 0 0
Cell Periphery 1 0 4 2 2 2 3 6 6 7 16 8 6 5 37 12 6 1 1 3 1 0 1
Cytoplasm 255 205 201 185 215 183 222 242 150 120 126 417 351 449 28 29 18 197 157 95 66 115 139
Endoplasmic Reticulum 7 14 10 11 22 13 18 12 20 20 39 5 5 7 1 0 2 18 28 56 4 3 6
Endosome 1 0 3 0 1 2 2 7 2 0 3 2 0 1 0 1 0 3 1 3 1 1 4
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 1 7 7 10 0 1 0 0 2 3
Mitochondria 10 9 14 22 14 103 165 225 216 174 204 0 1 0 11 2 3 29 13 47 10 15 13
Nucleus 0 0 0 0 0 3 2 4 1 5 2 0 0 1 2 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 1 0 0 1 3 4 3 2 13 0 0 0 3 1 1 0 0 0 0 0 2
Nucleolus 1 0 2 0 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 0 3 3 0 6 0 0 0 1 0 0 1 4 4 1 1 3
Vac/Vac Membrane 0 2 5 5 8 11 9 41 24 24 40 0 0 1 12 18 3 5 7 5 2 4 3
Unique Cell Count 271 223 225 207 257 261 345 446 336 283 351 425 357 458 105 70 41 278 234 237 94 151 193
Labelled Cell Count 279 231 245 226 264 325 431 556 439 366 477 434 365 467 113 78 45 278 234 237 94 151 193


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 4.8 4.5 4.0 4.8 3.8 4.1 4.0 3.7 4.2 4.8 4.9 5.0 5.2 14.0 14.3 19.3 5.4 5.5 6.2
Std Deviation (1e-4) 1.1 2.4 2.7 1.8 3.9 2.0 3.0 1.8 2.0 2.4 2.4 0.8 1.0 1.1 3.3 3.9 4.8 3.4 2.0 3.5
Intensity Change (Log2) -0.18 0.09 -0.24 -0.14 -0.18 -0.28 -0.12 0.08 0.13 0.15 0.19 1.63 1.66 2.09 0.27 0.28 0.46


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0
Bud Neck 0 0
Bud Site 0 0
Cell Periphery 0 0
Cyto
Endoplasmic Reticulum 0 0
Endosome 0 0
Golgi 0 0
Mitochondria 0 0
Nuclear Periphery 0 0
Nuc
Nucleolus 0 0
Peroxisomes 0 0
SpindlePole 0 0
Vac
Cortical Patches 0 0
Cytoplasm 0 0
Nucleus 0 0
Vacuole 3.5 6.4


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sam3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available