Standard name
Human Ortholog
Description S-adenosylmethionine-homocysteine methyltransferase; functions along with Mht1p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio; SAM4 has a paralog, YMR321C, that arose from a single-locus duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 1.0 1.0 0.96 0.97 0.96 0.85 0.87 0.77 0.82 0.69 0.79 1.0 0.99 0.99 0.98 0.96 0.99 0.98 0.92 0.95 0.95 0.98 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.13 0 0.25 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.06 0 0.13 0.11 0.09 0.11 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 5 1 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 1 0 0 0 1 3 0 6 3 0 0 0 0 0 0 0 1 0 1 0 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 1 1 3 3 8 4 7 11 5 8 0 2 3 8 7 8 0 0 0 0 0 0
Cytoplasm 243 133 127 141 209 222 223 286 178 196 150 176 162 262 279 303 302 219 232 139 129 99 187 234
Endoplasmic Reticulum 0 0 0 2 4 3 11 13 1 2 2 2 1 2 2 13 13 9 1 0 1 0 0 1
Endosome 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 1 0 1 0 0
Golgi 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 1 8 13 30 9 54 21 0 0 0 1 0 2 0 2 0 0 0 0
Nucleus 0 0 0 0 0 1 5 3 6 8 5 2 0 0 0 3 0 0 0 1 1 1 0 4
Nuclear Periphery 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 0 5 2 9 17 14 30 27 19 24 0 1 1 5 7 1 0 0 1 0 1 1
Unique Cell Count 244 133 127 147 215 232 263 329 231 240 216 223 162 265 281 308 313 222 237 151 137 105 192 249
Labelled Cell Count 245 133 128 150 218 239 274 339 258 253 241 240 163 267 285 333 331 240 237 151 137 105 192 249


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 41.0 49.2 33.7 28.6 23.6 17.5 14.0 12.7 9.9 9.9 8.3 8.4 34.2 32.9 32.3 42.7 47.0 45.3 39.5 37.2 38.8
Std Deviation (1e-4) 7.8 12.8 8.4 6.0 4.3 4.0 3.3 2.8 2.9 2.6 3.6 2.3 8.0 8.2 7.3 13.4 15.3 17.3 9.1 8.8 8.2
Intensity Change (Log2) – – – -0.23 -0.51 -0.94 -1.27 -1.41 -1.77 -1.77 -2.02 -2.01 0.02 -0.03 -0.06 0.34 0.48 0.43 0.23 0.14 0.21

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP7000204060WT3HU80HU120HU1600204060WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30204060WT1AF100AF140AF1800204060
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.3 -1.9 -2.4 -4.6 -4.3 -5.8 -5.1 -6.9 -5.6 0 -1.2 -1.0 -1.4 -2.1 -1.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 4.2 0 6.1 3.6 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 2.9 0 4.2 3.9 3.4 3.8 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 35.637 40.6847 40.0102 36.905 34.5815 37.5958 42.5058 48.5776 45.9209 46.361 42.2346 45.7957 41.8955 48.5491 44.2901 44.9371 45.2412 43.5537
Actin 0.0006 0.0003 0.0001 0.0001 0 0.0006 0.0002 0.0001 0.0002 0 0.0006 0.0001 0.0051 0.0001 0.0004 0.0001 0.0002 0.0004
Bud 0.0004 0.0001 0 0 0 0.001 0.0001 0.0003 0.0001 0 0.0003 0.0001 0.0004 0.0001 0.0003 0 0.0001 0.0008
Bud Neck 0.0004 0.0002 0.0002 0.0002 0.0004 0.0008 0.0001 0.0001 0.0002 0.0003 0.001 0.0005 0.0011 0.0001 0.0002 0.0002 0.0002 0.0006
Bud Periphery 0.0002 0 0 0 0 0.0003 0 0 0 0 0.0002 0 0.0005 0 0 0 0 0.0001
Bud Site 0.0004 0.0001 0 0 0 0.0003 0 0.0001 0.0001 0 0.0001 0 0.0028 0.0001 0.0001 0 0 0.0001
Cell Periphery 0.0001 0.0001 0 0 0 0 0 0.0001 0 0 0.0006 0 0.0004 0.0022 0 0 0 0
Cytoplasm 0.9335 0.987 0.9805 0.983 0.9726 0.9492 0.9778 0.9901 0.982 0.9245 0.9542 0.9671 0.9097 0.984 0.9785 0.9743 0.9704 0.9526
Cytoplasmic Foci 0.0013 0.0003 0.0002 0.0007 0.0006 0.007 0.0005 0.0001 0.0003 0.0001 0.0002 0.0004 0.0009 0.0003 0.0031 0.0002 0.0002 0.0038
Eisosomes 0 0 0 0 0 0 0 0 0 0 0 0 0.0004 0.0001 0 0 0 0
Endoplasmic Reticulum 0.0008 0.0003 0.0004 0.0003 0.0006 0.0007 0.0006 0.0005 0.0004 0.0002 0.0013 0.0006 0.0007 0.0002 0.0002 0.0001 0.0006 0.0007
Endosome 0.0003 0.0001 0.0001 0.0001 0.0004 0.0003 0.0001 0 0.0001 0 0.0001 0.0002 0.0013 0.0001 0.0001 0 0.0001 0.0009
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.0004 0 0 0 0 0.0001
Lipid Particles 0.0002 0 0 0 0 0.0001 0 0 0 0 0.0001 0 0.0008 0.0003 0.0001 0 0 0.0001
Mitochondria 0.0009 0.0001 0 0 0 0.0021 0.0001 0 0 0 0 0 0.0021 0.0001 0.0002 0 0.0001 0.0005
None 0.0019 0.0003 0.0001 0.0003 0.0003 0.0014 0.0004 0.0001 0.0003 0.0001 0.0002 0.0002 0.0016 0.0004 0.0009 0.0002 0.0002 0.0011
Nuclear Periphery 0.0071 0.0017 0.0023 0.0018 0.0038 0.0037 0.0025 0.0013 0.0018 0.0042 0.0067 0.0033 0.0066 0.0014 0.002 0.0026 0.0046 0.0051
Nucleolus 0.0029 0 0 0 0 0.0006 0 0 0 0 0.0001 0 0.0021 0.001 0.0003 0 0 0.0003
Nucleus 0.0456 0.0087 0.0156 0.0117 0.0197 0.0294 0.017 0.0068 0.014 0.0701 0.0312 0.0263 0.0572 0.0078 0.0122 0.0221 0.0231 0.0296
Peroxisomes 0.0001 0 0 0 0 0.0003 0 0 0 0 0 0 0.0005 0 0 0 0 0
Punctate Nuclear 0.0017 0.0003 0.0002 0.0015 0.0002 0.0014 0.0003 0.0001 0.0002 0.0002 0.0003 0.0003 0.0007 0.0001 0.0006 0.0001 0.0001 0.0015
Vacuole 0.0014 0.0003 0.0002 0.0002 0.0011 0.0006 0.0003 0.0002 0.0002 0.0002 0.0028 0.0006 0.0029 0.0015 0.0005 0.0002 0.0002 0.0012
Vacuole Periphery 0.0003 0 0 0.0001 0.0001 0.0001 0 0 0 0 0.0001 0.0001 0.0018 0.0001 0.0002 0.0001 0.0001 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 165.285 169.4389 172.88 195.5256 180.655 162.3745 173.104 176.5408 153.7678 199.8603
Translational Efficiency 1.1084 1.1004 1.0895 0.7538 1.098 1.1267 1.0632 0.9181 0.9997 0.9376

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
975 883 1332 946 1442 2081 288 909 2417 2964 1620 1855

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2765.79 3614.22 2897.33 2387.65 2779.26 3351.98 2706.17 2193.96 2773.83 3430.10 2863.35 2292.74
Standard Deviation 614.29 853.27 521.03 497.84 597.29 711.81 496.02 478.54 604.24 766.17 521.82 497.99
Intensity Change Log 2 — 0.385993 0.067032 -0.212100 — 0.270313 -0.038448 -0.341164 — 0.329171 0.015128 -0.275346

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000109 0.000094 0.000107 0.000393 0.000074 0.000077 0.000253 0.000323 0.000088 0.000082 0.000133 0.000359
Bud Neck 0.000567 0.000417 0.000852 0.002181 0.000379 0.000362 0.001993 0.006214 0.000455 0.000379 0.001055 0.004157
Bud Site 0.001048 0.000973 0.001146 0.006172 0.000490 0.001455 0.002077 0.016802 0.000715 0.001311 0.001311 0.011381
Cell Periphery 0.000306 0.000356 0.000268 0.000375 0.000174 0.000117 0.000463 0.000174 0.000227 0.000188 0.000302 0.000277
Cytoplasm 0.811811 0.721386 0.678660 0.720477 0.775480 0.769520 0.589319 0.716948 0.790136 0.755180 0.662777 0.718747
Cytoplasmic Foci 0.006821 0.005124 0.006757 0.011188 0.002933 0.003252 0.009366 0.008453 0.004502 0.003809 0.007221 0.009848
Eisosomes 0.000001 0.000001 0.000001 0.000004 0.000000 0.000001 0.000002 0.000004 0.000001 0.000001 0.000001 0.000004
Endoplasmic Reticulum 0.000046 0.000088 0.000035 0.000086 0.000034 0.000029 0.000075 0.000206 0.000039 0.000047 0.000043 0.000145
Endosome 0.000064 0.000138 0.000066 0.002399 0.000035 0.000154 0.000143 0.004739 0.000047 0.000149 0.000080 0.003546
Golgi 0.000598 0.000686 0.000219 0.003397 0.000371 0.000531 0.000377 0.002124 0.000462 0.000577 0.000247 0.002773
Lipid Particles 0.000507 0.000338 0.000536 0.001094 0.000259 0.000245 0.000905 0.001022 0.000359 0.000273 0.000601 0.001059
Mitochondria 0.000065 0.000255 0.000139 0.003240 0.000120 0.000385 0.000250 0.001475 0.000098 0.000346 0.000159 0.002375
Mitotic Spindle 0.000203 0.000570 0.001037 0.012144 0.000374 0.001001 0.003245 0.062127 0.000305 0.000873 0.001430 0.036637
None 0.013829 0.022579 0.010853 0.008581 0.015770 0.018370 0.012451 0.008444 0.014987 0.019624 0.011137 0.008514
Nuclear Periphery 0.000072 0.000108 0.000220 0.000287 0.000070 0.000061 0.000385 0.000822 0.000071 0.000075 0.000249 0.000550
Nuclear Periphery Foci 0.002224 0.000988 0.001146 0.002127 0.001138 0.000767 0.001788 0.003852 0.001576 0.000833 0.001260 0.002972
Nucleolus 0.000078 0.000260 0.000083 0.000161 0.000120 0.000093 0.000147 0.000307 0.000103 0.000143 0.000095 0.000232
Nucleus 0.155243 0.240513 0.288404 0.198505 0.198647 0.200363 0.364507 0.135609 0.181138 0.212324 0.301933 0.167685
Peroxisomes 0.000009 0.000174 0.000009 0.000097 0.000005 0.000017 0.000023 0.000297 0.000006 0.000063 0.000011 0.000195
Vacuole 0.006139 0.003711 0.009417 0.023327 0.003310 0.003022 0.012162 0.026323 0.004452 0.003227 0.009905 0.024795
Vacuole Periphery 0.000260 0.001241 0.000045 0.003765 0.000214 0.000180 0.000069 0.003735 0.000233 0.000496 0.000049 0.003750

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.06 -3.12 -4.98 -6.27 -3.46 0.66 -7.11 -3.84 -3.97 0.87 1.98 -8.50 -6.21 -6.87 -2.42
Bud Neck 2.70 -7.30 -7.05 -8.45 -1.99 0.26 -7.30 -4.97 -5.03 2.16 2.22 -11.76 -8.12 -8.77 -0.64
Bud Site 1.37 -6.09 -7.45 -7.53 -5.21 -2.17 -5.66 -6.12 -5.03 -2.42 -1.48 -10.13 -8.96 -7.51 -5.66
Cell Periphery -0.65 -2.79 -2.55 -1.79 -0.04 2.37 -6.45 -0.84 -3.42 6.26 1.99 -8.55 -3.52 -5.55 5.56
Cytoplasm 10.79 24.10 16.92 6.51 -4.32 2.09 19.96 11.11 10.12 -11.47 7.86 30.05 19.54 13.73 -9.54
Cytoplasmic Foci 1.18 -5.14 -4.44 -5.34 0.40 -0.64 -7.03 -8.96 -8.45 3.88 1.48 -10.92 -9.65 -10.76 3.06
Eisosomes -0.31 -7.82 -10.00 -7.17 -5.12 -0.16 -8.25 -11.01 -10.85 2.33 0.22 -13.23 -15.14 -14.06 -3.65
Endoplasmic Reticulum -0.48 -1.34 -2.43 -0.10 -1.68 0.94 -5.86 -2.91 -3.17 1.64 0.06 -5.85 -3.98 -2.57 -0.74
Endosome -0.50 -4.20 -2.53 -2.31 -2.19 -2.46 -5.87 -2.88 -2.57 -2.07 -2.22 -8.49 -3.81 -3.39 -3.18
Golgi -0.15 2.41 -0.59 -0.50 -1.31 -0.73 -2.19 -0.59 -0.01 0.22 -0.47 1.69 -0.90 -0.60 -1.35
Lipid Particles 2.27 -4.75 -7.08 -8.50 -2.86 0.79 -5.21 -9.05 -9.65 2.46 2.58 -8.54 -11.26 -12.99 -0.58
Mitochondria -1.35 -14.49 -1.11 -0.78 -0.93 -0.93 -5.43 -1.23 -0.01 -0.24 -1.27 -6.95 -1.39 -0.64 -1.07
Mitotic Spindle -1.23 -3.70 -4.03 -3.74 -1.72 -1.91 -2.98 -5.22 -5.00 -3.51 -2.27 -4.52 -6.28 -5.92 -4.40
None -4.32 3.41 6.35 8.70 6.27 -1.52 3.77 7.45 14.11 5.58 -3.95 5.69 9.63 16.41 8.25
Nuclear Periphery -1.30 -13.55 -10.30 -7.81 1.04 0.60 -10.11 -7.50 -7.62 3.76 -0.29 -17.03 -11.88 -11.55 2.01
Nuclear Periphery Foci 5.66 2.41 1.01 -4.66 -1.29 2.74 -4.21 -2.24 -4.14 2.12 6.05 -1.59 -1.45 -6.53 -0.07
Nucleolus -0.99 -5.05 -5.39 0.55 -2.81 0.18 -1.73 -1.34 -2.84 0.79 -0.81 -1.26 -2.37 -0.32 -2.91
Nucleus -11.79 -21.40 -6.41 5.29 13.84 -1.52 -13.03 6.69 8.60 16.33 -8.15 -23.27 0.48 8.01 22.57
Peroxisomes -0.96 -2.68 -3.67 0.74 -2.64 -2.79 -6.73 -2.68 -2.27 -1.27 -1.11 -6.21 -3.76 -0.08 -2.58
Vacuole 2.93 -10.01 -17.25 -18.61 -10.92 -0.02 -7.99 -19.19 -19.19 -3.87 2.85 -14.91 -26.18 -27.02 -13.28
Vacuole Periphery -1.33 0.94 -1.27 0.23 -1.63 0.21 0.98 -1.60 -1.71 -1.96 -1.12 1.52 -1.94 -1.05 -2.46
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description S-adenosylmethionine-homocysteine methyltransferase; functions along with Mht1p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio; SAM4 has a paralog, YMR321C, that arose from a single-locus duplication
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Sam4

Sam4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sam4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available