Standard name
Description S-adenosylmethionine-homocysteine methyltransferase; functions along with Mht1p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio; SAM4 has a paralog, YMR321C, that arose from a single-locus duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 1.0 1.0 0.96 0.97 0.96 0.85 0.87 0.77 0.82 0.69 0.79 1.0 0.99 0.99 0.98 0.96 0.99 0.98 0.92 0.95 0.95 0.98 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.13 0 0.25 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.06 0 0.13 0.11 0.09 0.11 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 5 1 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 1 0 0 0 1 3 0 6 3 0 0 0 0 0 0 0 1 0 1 0 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 1 1 3 3 8 4 7 11 5 8 0 2 3 8 7 8 0 0 0 0 0 0
Cytoplasm 243 133 127 141 209 222 223 286 178 196 150 176 162 262 279 303 302 219 232 139 129 99 187 234
Endoplasmic Reticulum 0 0 0 2 4 3 11 13 1 2 2 2 1 2 2 13 13 9 1 0 1 0 0 1
Endosome 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 1 0 1 0 0
Golgi 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 1 8 13 30 9 54 21 0 0 0 1 0 2 0 2 0 0 0 0
Nucleus 0 0 0 0 0 1 5 3 6 8 5 2 0 0 0 3 0 0 0 1 1 1 0 4
Nuclear Periphery 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 0 5 2 9 17 14 30 27 19 24 0 1 1 5 7 1 0 0 1 0 1 1
Unique Cell Count 244 133 127 147 215 232 263 329 231 240 216 223 162 265 281 308 313 222 237 151 137 105 192 249
Labelled Cell Count 245 133 128 150 218 239 274 339 258 253 241 240 163 267 285 333 331 240 237 151 137 105 192 249


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 41.0 49.2 33.7 28.6 23.6 17.5 14.0 12.7 9.9 9.9 8.3 8.4 34.2 32.9 32.3 42.7 47.0 45.3 39.5 37.2 38.8
Std Deviation (1e-4) 7.8 12.8 8.4 6.0 4.3 4.0 3.3 2.8 2.9 2.6 3.6 2.3 8.0 8.2 7.3 13.4 15.3 17.3 9.1 8.8 8.2
Intensity Change (Log2) – – – -0.23 -0.51 -0.94 -1.27 -1.41 -1.77 -1.77 -2.02 -2.01 0.02 -0.03 -0.06 0.34 0.48 0.43 0.23 0.14 0.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.3 -1.9 -2.4 -4.6 -4.3 -5.8 -5.1 -6.9 -5.6 0 -1.2 -1.0 -1.4 -2.1 -1.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 4.2 0 6.1 3.6 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 2.9 0 4.2 3.9 3.4 3.8 0 0 0 0 0 0