Standard name
Description G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p); CLN2 has a paralog, CLN1, that arose from the whole genome duplication; cell cycle arrest phenotype of the cln1 cln2 cln3 triple null mutant is complemented by any of human cyclins CCNA2, CCNB1, CCNC, CCND1, or CCNE1

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.83 0.86 0.95 0.93 0.91 0.69 0.71 0.69 0.55 0.5 0.53 0.46 0.99 0.99 1.0 0.93 0.91 0.94 0.66 0.69 0.63 0.81 0.79 0.72
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.08 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.14 0 0 0.16 0.13 0.5 0.47 0.45 0.65 0.7 0.66 0.75 0 0 0 0.06 0 0 0.08 0.06 0.17 0.05 0.06 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Vac/Vac Membrane 0.05 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.07 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 3 9 2 6 5
Bud 2 1 2 3 0 7 11 11 10 17 10 5 1 0 0 1 0 0 5 11 9 4 12 15
Bud Neck 1 1 1 0 0 2 1 6 2 4 1 2 1 0 0 6 2 0 1 3 4 2 4 7
Bud Site 0 1 0 0 0 0 0 1 2 1 1 2 0 0 0 0 0 0
Cell Periphery 4 2 4 1 2 0 3 1 1 3 1 4 1 1 1 1 4 1 0 0 7 1 5 5
Cytoplasm 208 246 385 238 280 353 426 477 301 273 189 171 410 464 462 297 226 161 206 209 264 217 332 257
Endoplasmic Reticulum 0 4 0 0 1 1 1 1 1 3 1 1 1 0 0 27 19 11 4 0 3 0 1 3
Endosome 6 0 2 0 2 3 3 2 1 1 0 0 0 2 0 4 3 3 2 2 5 5 3 4
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 0 2 1 2 2 2 2 1 2 1 3
Mitochondria 36 6 9 41 40 255 284 310 354 384 238 279 0 1 0 19 2 4 24 18 71 12 23 17
Nucleus 4 2 1 0 0 2 4 4 4 0 2 3 1 1 1 4 7 3 13 18 5 0 0 0
Nuclear Periphery 1 0 0 0 0 0 0 1 1 2 1 0 0 0 0 0 0 0 1 0 1 0 0 0
Nucleolus 3 1 0 0 0 1 1 1 0 1 1 0 0 1 1 0 0 0 1 0 0 0 0 0
Peroxisomes 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 2 1 1 1 1 3 12 19 14 4 10 6 0 1 0 1 2 0 12 14 6 5 11 10
Vac/Vac Membrane 12 30 12 7 4 5 9 9 9 4 2 3 5 1 0 14 16 7 22 14 14 4 6 15
Unique Cell Count 251 286 405 257 308 508 602 689 543 549 358 372 415 470 464 321 248 171 312 306 420 268 422 359
Labelled Cell Count 279 295 417 291 333 632 755 843 700 697 457 476 420 472 467 375 283 192 312 306 420 268 422 359


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 4.3 3.9 3.6 3.6 3.2 2.9 3.1 2.9 2.7 2.8 2.7 4.3 4.2 4.2 5.2 5.9 6.0 4.0 4.3 4.9
Std Deviation (1e-4) 0.8 1.0 1.1 1.4 1.3 1.0 1.1 1.2 1.0 0.8 1.2 1.3 1.4 2.0 1.5 1.6 1.8 1.9 1.6 2.5 1.9
Intensity Change (Log2) -0.13 -0.12 -0.31 -0.46 -0.34 -0.45 -0.55 -0.46 -0.55 0.12 0.12 0.09 0.42 0.58 0.61 0.02 0.14 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 1.0 2.1 0.7


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cln2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available