Standard name
Human Ortholog
Description G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p); CLN2 has a paralog, CLN1, that arose from the whole genome duplication; cell cycle arrest phenotype of the cln1 cln2 cln3 triple null mutant is complemented by any of human cyclins CCNA2, CCNB1, CCNC, CCND1, or CCNE1
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.83 0.86 0.95 0.93 0.91 0.69 0.71 0.69 0.55 0.5 0.53 0.46 0.99 0.99 1.0 0.93 0.91 0.94 0.66 0.69 0.63 0.81 0.79 0.72
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.08 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.14 0 0 0.16 0.13 0.5 0.47 0.45 0.65 0.7 0.66 0.75 0 0 0 0.06 0 0 0.08 0.06 0.17 0.05 0.06 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Vac/Vac Membrane 0.05 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.07 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 3 9 2 6 5
Bud 2 1 2 3 0 7 11 11 10 17 10 5 1 0 0 1 0 0 5 11 9 4 12 15
Bud Neck 1 1 1 0 0 2 1 6 2 4 1 2 1 0 0 6 2 0 1 3 4 2 4 7
Bud Site 0 1 0 0 0 0 0 1 2 1 1 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 2 4 1 2 0 3 1 1 3 1 4 1 1 1 1 4 1 0 0 7 1 5 5
Cytoplasm 208 246 385 238 280 353 426 477 301 273 189 171 410 464 462 297 226 161 206 209 264 217 332 257
Endoplasmic Reticulum 0 4 0 0 1 1 1 1 1 3 1 1 1 0 0 27 19 11 4 0 3 0 1 3
Endosome 6 0 2 0 2 3 3 2 1 1 0 0 0 2 0 4 3 3 2 2 5 5 3 4
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 0 2 1 2 2 2 2 1 2 1 3
Mitochondria 36 6 9 41 40 255 284 310 354 384 238 279 0 1 0 19 2 4 24 18 71 12 23 17
Nucleus 4 2 1 0 0 2 4 4 4 0 2 3 1 1 1 4 7 3 13 18 5 0 0 0
Nuclear Periphery 1 0 0 0 0 0 0 1 1 2 1 0 0 0 0 0 0 0 1 0 1 0 0 0
Nucleolus 3 1 0 0 0 1 1 1 0 1 1 0 0 1 1 0 0 0 1 0 0 0 0 0
Peroxisomes 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 2 1 1 1 1 3 12 19 14 4 10 6 0 1 0 1 2 0 12 14 6 5 11 10
Vac/Vac Membrane 12 30 12 7 4 5 9 9 9 4 2 3 5 1 0 14 16 7 22 14 14 4 6 15
Unique Cell Count 251 286 405 257 308 508 602 689 543 549 358 372 415 470 464 321 248 171 312 306 420 268 422 359
Labelled Cell Count 279 295 417 291 333 632 755 843 700 697 457 476 420 472 467 375 283 192 312 306 420 268 422 359


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 4.3 3.9 3.6 3.6 3.2 2.9 3.1 2.9 2.7 2.8 2.7 4.3 4.2 4.2 5.2 5.9 6.0 4.0 4.3 4.9
Std Deviation (1e-4) 0.8 1.0 1.1 1.4 1.3 1.0 1.1 1.2 1.0 0.8 1.2 1.3 1.4 2.0 1.5 1.6 1.8 1.9 1.6 2.5 1.9
Intensity Change (Log2) – – – -0.13 -0.12 -0.31 -0.46 -0.34 -0.45 -0.55 -0.46 -0.55 0.12 0.12 0.09 0.42 0.58 0.61 0.02 0.14 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 1.0 2.1 0.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.1784 0.2094 0.1632 -0.8148 -0.5884 -0.4428 3.1674 1.7193 1.4917 0.592 1.5215 1.8673 2.2883 3.0348 2.6494 2.2956 2.2523 2.7468
Actin 0.0406 0.0012 0.0144 0.0007 0.0035 0.0003 0.0522 0.0109 0.0008 0.003 0.1419 0.0105 0.0355 0.0031 0.01 0.0003 0.0015 0.0047
Bud 0.0021 0.0008 0.0004 0.0022 0.0001 0.0004 0.0028 0.0002 0.0158 0.0092 0.0008 0.0028 0.001 0.0003 0.002 0.0003 0.0006 0.001
Bud Neck 0.0033 0.0006 0.0024 0.0005 0.0002 0.0033 0.004 0.0001 0.0002 0 0.0018 0.0024 0.0054 0.0005 0.0005 0.0001 0.0221 0.0024
Bud Periphery 0.0037 0.0011 0.0006 0.0053 0.0001 0.0005 0.0033 0.0003 0.0026 0.0357 0.0015 0.004 0.0027 0.0005 0.0033 0.0003 0.0012 0.001
Bud Site 0.0218 0.0048 0.0042 0.0017 0.0001 0.0001 0.0131 0.0011 0.0058 0.0001 0.0014 0.0015 0.0055 0.005 0.0024 0.0001 0.0121 0.0038
Cell Periphery 0.0013 0.0003 0.0005 0.0002 0.0001 0.0001 0.0005 0.0001 0.0001 0.0001 0.0001 0.0004 0.0005 0.0002 0.0002 0 0.0006 0.0001
Cytoplasm 0.1173 0.2396 0.1915 0.1022 0.0204 0.1409 0.018 0.1839 0.1502 0.0521 0.0464 0.1217 0.0298 0.175 0.1354 0.0821 0.0454 0.1334
Cytoplasmic Foci 0.05 0.0077 0.0074 0.0142 0.0069 0.0109 0.0724 0.0044 0.0109 0.0013 0.0271 0.0131 0.0105 0.0056 0.0074 0.0038 0.0347 0.0058
Eisosomes 0.0041 0.0001 0.0005 0.0002 0.0004 0.0001 0.0006 0.0002 0.0001 0.0002 0.0006 0.0001 0.0006 0.0002 0.0002 0 0.0004 0.0001
Endoplasmic Reticulum 0.0248 0.0057 0.0025 0.0009 0.0003 0.0016 0.0193 0.0022 0.0014 0.0008 0.0007 0.0036 0.0049 0.0012 0.0031 0.0014 0.0003 0.0007
Endosome 0.0512 0.021 0.0039 0.0019 0.0057 0.0033 0.0672 0.0009 0.0009 0.0004 0.0069 0.0099 0.0109 0.0013 0.0029 0.0037 0.0016 0.0026
Golgi 0.0227 0.003 0.003 0.0002 0.0107 0.0002 0.0237 0.0009 0.0002 0.0001 0.0121 0.0025 0.0042 0.0003 0.0009 0.0001 0.0002 0.0005
Lipid Particles 0.0378 0.0013 0.0043 0.0006 0.0102 0.0029 0.0726 0.0011 0.0003 0.0001 0.0148 0.0007 0.0096 0.0007 0.0008 0.0004 0.001 0.0007
Mitochondria 0.0233 0.0013 0.0091 0.0003 0.0144 0.0003 0.0118 0.0013 0.0007 0.007 0.0008 0.0027 0.0027 0.0004 0.0006 0.0003 0.0004 0.0009
None 0.5013 0.563 0.6547 0.8643 0.9211 0.7349 0.4595 0.7806 0.7635 0.8724 0.7042 0.8124 0.831 0.7733 0.7965 0.8874 0.8685 0.7931
Nuclear Periphery 0.024 0.0036 0.0018 0.0004 0.0002 0.0025 0.0501 0.001 0.0014 0.0099 0.0016 0.0018 0.0117 0.0009 0.0024 0.0027 0.0003 0.0011
Nucleolus 0.0036 0.0015 0.0013 0.0002 0.0001 0.0014 0.0045 0.0001 0.0009 0.0005 0.0002 0.001 0.0013 0.0006 0.0007 0.0003 0.0007 0.0015
Nucleus 0.0151 0.13 0.0819 0.0006 0.0002 0.0835 0.0152 0.0039 0.0393 0.0035 0.0003 0.0038 0.0043 0.0262 0.021 0.0072 0.0008 0.0421
Peroxisomes 0.0244 0.0002 0.0106 0.0008 0.0041 0.0004 0.0737 0.0055 0.0011 0.0004 0.0233 0.0005 0.0141 0.0009 0.0032 0.0001 0.0054 0.0005
Punctate Nuclear 0.0149 0.007 0.0019 0.0015 0.0005 0.0101 0.0248 0.0009 0.003 0.0013 0.0131 0.0028 0.0117 0.0028 0.0049 0.0084 0.0015 0.0024
Vacuole 0.0102 0.0055 0.0029 0.0008 0.0003 0.0021 0.0052 0.0004 0.0007 0.0006 0.0002 0.0011 0.0012 0.001 0.0014 0.0007 0.0004 0.0012
Vacuole Periphery 0.0024 0.0005 0.0004 0.0001 0.0003 0.0002 0.0053 0.0001 0.0001 0.0013 0.0001 0.0007 0.001 0.0001 0.0002 0.0001 0.0001 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 91.2767 24.0873 10.2775 25.9327 113.1039 67.9989 21.4626 11.0955 17.1608 130.7192
Translational Efficiency 1.0578 0.7939 0.5245 0.4718 0.9077 1.5939 0.8361 0.7748 0.5279 0.728

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✔
Standard name
Human Ortholog
Description G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p); CLN2 has a paralog, CLN1, that arose from the whole genome duplication; cell cycle arrest phenotype of the cln1 cln2 cln3 triple null mutant is complemented by any of human cyclins CCNA2, CCNB1, CCNC, CCND1, or CCNE1
Localization
Cell Percentages cytoplasm (12%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Cln2

Cln2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cln2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available