Standard name
Human Ortholog
Description GTPase-activating protein (GAP) for yeast Rab family members; involved in ER to Golgi trafficking; exhibits GAP activity toward Ypt1p that is stimulated by Gyl1p, also acts on Sec4p; interacts with Gyl1p, Rvs161p and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth; relocalizes from bud neck to cytoplasm upon DNA replication stress; GYP5 has a paralog, GYL1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.16 0.12 0 0.07 0.05 0.08 0 0 0 – 0 0 0 0 0 0.07 0.09 0.09 0.15 0.12 0.1 0.15 0.15 0.14
Bud 0.08 0.07 0 0 0.06 0.08 0.05 0.06 0 – 0.07 0.06 0 0 0 0 0 0 0.12 0.1 0 0.16 0.28 0.24
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.12 0.14 0.06 0.14 0.08 0.08
Bud Site 0.07 0.06 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0.05 0 0 0 0 0 – 0 0.05 0 0.05 0.05 0.05 0.09 0.09 0 0 0 0 0 0
Cytoplasm 0.73 0.66 0.85 0.81 0.83 0.76 0.8 0.78 0.76 – 0.67 0.68 0.92 0.99 0.97 0.72 0.74 0.78 0.4 0.4 0.5 0.24 0.24 0.32
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0.05 0.05 0 0 0 0 0 0
Endosome 0 0.07 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0.06 0 0.05 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.1 0.13 0.09 0.23 – 0.41 0.29 0 0 0 0 0 0 0 0.06 0.11 0.09 0.07 0.05
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0.1 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.05 0.08 0.08 0.1 0.12 0 0.05 0.08 0 – 0 0.05 0 0 0 0 0.05 0 0 0.05 0.07 0 0.06 0.06
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.13 0.07 0.1 0 0 0 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 40 35 10 14 13 22 17 15 9 – 5 5 6 0 1 22 18 21 44 41 27 25 68 57
Bud 19 19 3 8 14 23 19 23 6 – 19 15 7 2 6 5 0 2 35 34 10 27 125 94
Bud Neck 0 3 7 2 2 2 2 10 4 – 0 2 0 0 0 0 0 0 35 48 15 23 35 32
Bud Site 16 17 6 7 10 12 10 6 2 – 3 2 4 0 1 6 1 3 – – – – – –
Cell Periphery 3 7 8 10 8 1 4 6 11 – 8 11 13 19 20 17 18 22 1 3 3 1 3 4
Cytoplasm 178 192 212 173 201 213 309 291 224 – 173 165 308 368 428 224 153 189 117 132 133 40 108 127
Endoplasmic Reticulum 7 8 4 4 1 3 6 5 0 – 2 2 2 1 1 15 11 11 6 3 3 4 9 8
Endosome 3 19 0 2 1 1 4 2 4 – 0 2 7 3 4 11 6 3 17 18 7 7 13 11
Golgi 0 2 0 0 0 0 0 0 0 – 0 0 0 0 0 3 0 5 0 2 1 0 2 3
Mitochondria 6 9 0 2 2 29 49 33 69 – 106 69 5 1 0 11 3 4 10 20 29 15 31 20
Nucleus 1 0 1 1 3 1 4 6 2 – 2 2 0 0 2 4 1 3 2 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 1 0 0 1 – 2 0 0 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 0 1 0 0 0 0 0 0 0 – 2 0 1 0 1 1 0 0 0 0 0 0 1 0
Peroxisomes 10 29 0 5 2 4 3 2 0 – 0 2 11 0 3 6 3 2 0 0 0 3 6 3
SpindlePole 12 22 21 21 28 11 20 30 6 – 6 12 8 1 2 13 10 8 8 15 18 6 26 22
Vac/Vac Membrane 0 5 3 0 2 1 3 7 8 – 3 4 1 0 2 40 14 23 3 7 6 8 12 9
Unique Cell Count 245 289 248 214 243 279 386 374 296 260 242 333 373 443 312 207 241 291 333 268 168 450 400
Labelled Cell Count 295 368 275 249 287 324 450 436 346 331 293 373 395 471 378 238 296 291 333 268 168 450 400


Ambiguous, Bud, Bud Neck, Cell Periphery, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 5.9 6.5 5.8 6.5 4.9 5.5 5.6 5.0 – 4.4 5.1 5.8 6.0 6.1 9.5 10.3 9.7 5.4 5.4 5.9
Std Deviation (1e-4) 0.8 0.8 3.2 2.1 2.0 0.9 1.6 1.6 2.2 – 1.3 1.4 1.1 1.2 1.6 1.6 1.6 1.7 1.3 1.4 1.7
Intensity Change (Log2) – – – -0.18 -0.0 -0.42 -0.25 -0.22 -0.39 – -0.56 -0.35 -0.18 -0.12 -0.09 0.53 0.65 0.57 -0.28 -0.27 -0.15


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.2 0.7 1.8 0.2 0 -0.6 – 0 -1.3 -1.6 0 0 1.5 2.1 2.1
Bud 1.8 2.8 3.7 2.5 3.0 0 – 0 2.9 0 0 0 0 0 0
Bud Neck 0 0 0 0 -0.1 -1.2 – 0 0 0 0 0 0 0 0
Bud Site 0.6 1.1 1.2 0.1 0 0 – 0 0 0 0 0 -0.4 0 0
Cell Periphery 0.8 0 0 0 -1.3 0.3 – 0 0.8 0.4 1.1 0.8 1.3 2.5 2.7
Cytoplasm -1.3 -0.8 -2.7 -1.7 -2.4 -2.9 – -5.0 -4.5 2.7 6.5 5.4 -3.9 -3.1 -2.0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 2.1 2.2 1.9
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 5.2 5.8 4.8 8.1 – 0 9.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0.5 1.1 -2.2 -1.6 -0.2 -3.4 – 0 -1.5 -3.3 -5.4 -5.6 -2.1 -1.5 -2.4
Vacuole 0 0 0 0 0 1.2 – 0 0 0 0 0 5.1 3.1 4.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.1081 2.999 2.4242 2.7227 1.9248 2.4565 1.8439 2.0791 1.8379 1.4922 1.9289 1.7796 1.7884 1.6159 1.4484 1.3861 1.3294 1.4831
Actin 0.0635 0.01 0.0696 0.0279 0.0454 0.0252 0.0751 0.0159 0.0437 0.0169 0.0365 0.0304 0.0274 0.0167 0.0334 0.0079 0.0463 0.0204
Bud 0.0222 0.0506 0.0583 0.0134 0.0086 0.006 0.025 0.0298 0.0725 0.0336 0.0167 0.017 0.0196 0.0455 0.0977 0.024 0.0407 0.0199
Bud Neck 0.0192 0.0103 0.0132 0.0014 0.0135 0.3573 0.0284 0.0048 0.0112 0.0274 0.0021 0.3448 0.032 0.0033 0.0076 0.0012 0.0422 0.3571
Bud Periphery 0.0239 0.0396 0.0683 0.0686 0.0086 0.0025 0.038 0.0259 0.0955 0.0725 0.0455 0.0181 0.0215 0.0248 0.1067 0.0341 0.0701 0.0204
Bud Site 0.0876 0.3449 0.0435 0.0038 0.0252 0.0021 0.0818 0.2196 0.0771 0.0219 0.0081 0.0126 0.1085 0.2754 0.0532 0.0065 0.02 0.0102
Cell Periphery 0.0051 0.0031 0.004 0.0023 0.0027 0.0018 0.0025 0.0024 0.0015 0.0016 0.0007 0.0013 0.002 0.0026 0.0015 0.0011 0.0023 0.0013
Cytoplasm 0.3099 0.3616 0.3504 0.4072 0.3801 0.3702 0.3968 0.469 0.4379 0.549 0.4207 0.3492 0.3762 0.3611 0.3559 0.6486 0.3266 0.2931
Cytoplasmic Foci 0.1113 0.0467 0.0744 0.0812 0.0714 0.0696 0.085 0.0552 0.0445 0.0479 0.0475 0.1054 0.1223 0.069 0.0412 0.0195 0.0765 0.0942
Eisosomes 0.0019 0.0001 0.001 0.0003 0.0009 0.0003 0.0006 0.0001 0.0003 0.0001 0.0003 0.0001 0.0004 0.0002 0.0003 0.0001 0.0003 0.0002
Endoplasmic Reticulum 0.0046 0.0013 0.0033 0.0055 0.0051 0.0024 0.0056 0.0019 0.0036 0.0027 0.0046 0.0017 0.0066 0.0017 0.0041 0.0031 0.0068 0.0013
Endosome 0.0179 0.012 0.0221 0.0274 0.0331 0.0049 0.0321 0.0099 0.0188 0.0096 0.0387 0.0123 0.0342 0.0081 0.0187 0.0136 0.0391 0.0052
Golgi 0.0097 0.0023 0.0061 0.0064 0.0068 0.0034 0.0171 0.0028 0.007 0.0039 0.0255 0.0053 0.0123 0.002 0.0073 0.0028 0.0135 0.003
Lipid Particles 0.0092 0.0013 0.006 0.0123 0.0076 0.0039 0.0252 0.0007 0.0044 0.0013 0.0194 0.0008 0.008 0.0006 0.0039 0.0003 0.0153 0.0011
Mitochondria 0.0033 0.0016 0.0074 0.0082 0.0028 0.0009 0.0121 0.0014 0.0054 0.0043 0.0293 0.0048 0.0088 0.0014 0.0111 0.0027 0.0173 0.0032
None 0.2703 0.0963 0.2351 0.2874 0.3536 0.1347 0.1183 0.1312 0.145 0.1761 0.2729 0.0768 0.1474 0.1567 0.1979 0.2085 0.2079 0.134
Nuclear Periphery 0.0011 0.0003 0.0011 0.0013 0.0046 0.0002 0.007 0.0006 0.0014 0.001 0.0029 0.0006 0.0121 0.0012 0.005 0.0025 0.0053 0.0011
Nucleolus 0.0008 0.0009 0.0008 0.0005 0.0008 0.0004 0.0009 0.0013 0.001 0.0024 0.0005 0.0005 0.0022 0.0014 0.0016 0.0008 0.0009 0.0014
Nucleus 0.001 0.001 0.0014 0.0017 0.0024 0.0014 0.004 0.0039 0.0033 0.0037 0.0044 0.0021 0.0125 0.0059 0.0156 0.0069 0.0065 0.0047
Peroxisomes 0.0299 0.001 0.0228 0.0247 0.0148 0.0083 0.03 0.0086 0.0155 0.0054 0.0094 0.0102 0.0279 0.0069 0.0187 0.0013 0.0314 0.0138
Punctate Nuclear 0.0046 0.0128 0.0052 0.0033 0.0062 0.0034 0.0037 0.0035 0.0044 0.0118 0.003 0.0027 0.0099 0.0121 0.0098 0.0035 0.0243 0.0119
Vacuole 0.0027 0.0023 0.0053 0.0142 0.005 0.0009 0.0095 0.01 0.005 0.0061 0.0096 0.0031 0.0066 0.0033 0.006 0.0092 0.0055 0.0021
Vacuole Periphery 0.0003 0.0002 0.0006 0.0011 0.0009 0.0001 0.0014 0.0012 0.0009 0.0009 0.0017 0.0004 0.0017 0.0004 0.0027 0.002 0.0013 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.3833 4.7758 16.0835 18.1279 10.3899 13.5539 12.9076 20.808 24.0133 17.1072
Translational Efficiency 2.0122 4.3817 1.6205 2.0017 2.202 2.1876 1.6401 1.2727 1.4236 1.5047

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘