Standard name
Human Ortholog
Description Hsp90 chaperone; redundant in function with Hsc82p; required for pheromone signaling, negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; protein abundance increases in response to DNA replication stress; contains two acid-rich unstructured regions that promote solubility of chaperone-substrate complexes; HSP82 has a paralog, HSC82, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.14 0 0 0 0 0 0 0
Cytoplasm 1.0 1.0 1.0 0.98 0.93 0.95 0.88 0.82 0.89 0.77 0.83 0.99 0.99 1.0 0.95 0.94 1.0 0.98 0.94 0.97 0.93 0.9 0.85
Endoplasmic Reticulum 0 0 0 0 0.05 0 0.1 0.14 0.08 0.18 0.13 0 0 0 0 0.06 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 4 2 10 4 6 7 5 16 6 0 0 0 1 1 0 2 3 2 0 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 4 4 10 4 14 15 12 13 6 11 7 0 0 0 9 10 1 0 1 0 0 0 0
Cytoplasm 394 326 456 236 349 480 450 363 334 323 259 138 155 144 89 68 41 382 316 460 40 111 138
Endoplasmic Reticulum 0 1 0 2 17 14 51 63 31 77 40 0 0 0 3 4 3 0 1 2 0 2 5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleus 0 0 0 0 0 0 0 0 2 1 0 1 0 0 0 0 0 1 4 1 0 0 2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0
Nucleolus 0 0 0 0 0 0 0 4 1 5 4 0 1 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 2 2 0 0 0 1 0 2 3 0 0 0 0 0 0 0 4 2 0 6 2
Unique Cell Count 394 327 458 241 375 504 510 444 377 422 313 139 157 144 94 72 41 389 338 475 44 125 163
Labelled Cell Count 398 332 472 246 390 513 519 451 381 435 320 139 157 144 102 83 45 389 338 475 44 125 163


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 144.5 133.0 135.2 108.9 144.7 146.5 151.9 152.7 143.2 132.7 145.7 133.8 182.5 203.9 404.3 415.6 361.0 171.1 252.7
Std Deviation (1e-4) 48.9 53.6 50.8 51.0 48.5 41.8 46.1 49.9 50.3 46.2 53.3 44.8 58.4 64.5 159.3 135.0 165.8 57.1 108.9
Intensity Change (Log2) -0.31 0.1 0.12 0.17 0.18 0.08 -0.03 0.11 -0.01 0.43 0.59 1.58 1.62 1.42 0.34 0.9


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 2.0 0 0 1.0 0 2.9 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1.3 0.8 0.2 0.7 -0.6 0.4 0 0 0 0 0 0 0
Cytoplasm -2.1 -5.2 -4.1 -7.2 -9.2 -7.0 -10.7 -8.8 -0.4 -1.1 0.8 -3.9 -3.8 0.4
Endoplasmic Reticulum 0 4.6 3.6 7.0 8.4 6.3 9.6 7.9 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 56.1238 88.976 72.9336 61.6881 72.6916 69.1789 77.0377 111.1848 101.1799 86.8495 76.2118 107.871 71.6126 103.5199 86.5988 88.3481 75.4265 91.4043
Actin 0.0122 0.0003 0.0001 0.0001 0.0023 0.0085 0.0043 0.0002 0.0009 0.001 0.0053 0.0001 0.0055 0.0001 0.0004 0.0001 0.0045 0.0092
Bud 0.0019 0.0031 0.0005 0.0002 0.0006 0.0005 0.0007 0 0.0002 0.0001 0.0028 0.0001 0.0005 0.0001 0.0006 0.0002 0.0009 0.0001
Bud Neck 0.0008 0.0001 0.0003 0.0003 0.0008 0.0007 0.0004 0.0001 0.0001 0.0002 0.0004 0.0008 0.0002 0.0001 0.0001 0.0002 0.0005 0.0003
Bud Periphery 0.0017 0.0005 0.0003 0.0001 0.002 0.0005 0.0004 0 0.0002 0.0001 0.0035 0.0001 0.0004 0 0.0006 0.0001 0.0006 0.0001
Bud Site 0.0021 0.0002 0.0003 0.0001 0.0018 0.0003 0.0009 0.0002 0.0001 0.0002 0.0025 0.0001 0.0005 0.0001 0.0001 0.0004 0.0009 0
Cell Periphery 0.0014 0.0001 0.0002 0.0004 0.003 0.0001 0.001 0.0001 0.0012 0.0002 0.0029 0.0002 0.0006 0.0001 0.0003 0.0003 0.0006 0
Cytoplasm 0.845 0.9912 0.9779 0.9375 0.9197 0.9157 0.94 0.9918 0.9826 0.925 0.8404 0.9826 0.9312 0.9896 0.961 0.9674 0.8602 0.9777
Cytoplasmic Foci 0.0176 0.0004 0.0046 0.0093 0.0128 0.0112 0.009 0.0022 0.0003 0.0162 0.0112 0.0036 0.009 0.0003 0.0076 0.0076 0.0219 0.0005
Eisosomes 0.0002 0 0 0 0.0002 0.0001 0.0001 0.0001 0.0003 0.0002 0.0013 0 0.0001 0 0 0 0.0006 0
Endoplasmic Reticulum 0.003 0.0002 0.0003 0.0017 0.0089 0.0017 0.0015 0.0002 0.0005 0.0005 0.0038 0.0005 0.0019 0.0002 0.0006 0.0004 0.0012 0.0006
Endosome 0.0175 0 0.0003 0.0024 0.0033 0.0082 0.005 0.0001 0.0002 0.0024 0.0064 0.0004 0.004 0 0.001 0.0009 0.012 0.0009
Golgi 0.0057 0 0.0001 0.0001 0.0007 0.0021 0.0017 0.0001 0.0003 0.0006 0.0013 0 0.0014 0 0.0004 0.0001 0.0015 0.0008
Lipid Particles 0.0095 0 0.0009 0.0012 0.0059 0.0027 0.0035 0.0014 0.0015 0.0064 0.0052 0.0006 0.0058 0 0.0085 0.0015 0.0059 0.0001
Mitochondria 0.0081 0.0003 0.0018 0.0013 0.0021 0.0058 0.0064 0.0007 0.0062 0.0021 0.0371 0.0003 0.0043 0.0006 0.0032 0.0027 0.0328 0.0002
None 0.0124 0.0003 0.0012 0.0162 0.0091 0.004 0.0041 0.0003 0.0004 0.0128 0.0186 0.0008 0.005 0.0002 0.0037 0.006 0.0186 0.0004
Nuclear Periphery 0.0092 0.0005 0.0007 0.0043 0.0056 0.0109 0.0029 0.0005 0.0007 0.0028 0.0079 0.0009 0.0054 0.0012 0.001 0.0007 0.0024 0.004
Nucleolus 0.0071 0 0.004 0.0023 0.0024 0.0009 0.0017 0.0002 0.0001 0.0073 0.011 0.0022 0.0042 0.0042 0.0015 0.0029 0.0049 0
Nucleus 0.0165 0.0015 0.0023 0.004 0.0051 0.0071 0.0052 0.0011 0.0014 0.0039 0.0087 0.0035 0.008 0.0027 0.0018 0.0027 0.0065 0.0037
Peroxisomes 0.0054 0 0.0002 0.0001 0.0005 0.0011 0.0008 0.0002 0.0001 0.0071 0.0018 0.0001 0.0043 0 0.0041 0.0004 0.003 0
Punctate Nuclear 0.0021 0 0.0004 0.0014 0.0019 0.0027 0.0012 0.0001 0 0.0078 0.0007 0.0005 0.0015 0.0001 0.0003 0.0006 0.0038 0.0006
Vacuole 0.0151 0.0007 0.0024 0.0119 0.0093 0.0104 0.0072 0.0003 0.0016 0.0015 0.0171 0.002 0.0044 0.0002 0.0023 0.0032 0.0127 0.0003
Vacuole Periphery 0.0055 0.0002 0.001 0.0049 0.0022 0.0047 0.0019 0.0001 0.0009 0.0016 0.0099 0.0006 0.0018 0.0001 0.0009 0.0015 0.0039 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 372.9818 602.4767 633.6553 502.567 529.2593 507.9063 615.0164 675.0226 833.4645 461.4782
Translational Efficiency 1.4463 1.6276 1.2102 2.1699 1.5838 1.0934 1.4781 1.7678 1.3955 1.5657

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
857 1382 1869 120 1779 1477 2132 15 2636 2859 4001 135

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 11417.51 8605.48 11703.10 8364.51 6296.77 7715.97 17158.80 10487.21 7961.59 8145.95 14610.26 8600.37
Standard Deviation 3715.48 3389.85 5609.34 2529.01 1891.83 3244.98 5606.72 2101.04 3557.68 3345.46 6233.62 2573.08
Intensity Change Log 2 -0.407920 0.035643 -0.448895 0.293236 1.446265 0.735947 -0.118144 0.704253 0.089783

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000082 0.000078 0.000071 0.000109 0.000043 0.000076 0.000057 0.000613 0.000055 0.000077 0.000063 0.000165
Bud Neck 0.000887 0.000371 0.002237 0.003110 0.000430 0.000433 0.000837 0.003641 0.000578 0.000403 0.001491 0.003169
Bud Site 0.002567 0.000640 0.005045 0.001676 0.001151 0.001270 0.002000 0.037881 0.001611 0.000965 0.003422 0.005698
Cell Periphery 0.003145 0.002197 0.000662 0.012354 0.001466 0.001099 0.001170 0.010319 0.002011 0.001630 0.000933 0.012128
Cytoplasm 0.800906* 0.723046* 0.760578* 0.586109* 0.799317* 0.682804* 0.591214* 0.620169* 0.799833* 0.702257* 0.670330* 0.589893*
Cytoplasmic Foci 0.006311 0.004967 0.009362 0.039484 0.004393 0.007215 0.003824 0.032087 0.005016 0.006128 0.006411 0.038662
Eisosomes 0.000001 0.000001 0.000001 0.000007 0.000001 0.000001 0.000001 0.000005 0.000001 0.000001 0.000001 0.000007
Endoplasmic Reticulum 0.000037 0.000025 0.000032 0.000013 0.000029 0.000027 0.000025 0.000051 0.000032 0.000026 0.000028 0.000017
Endosome 0.000048 0.000018 0.000092 0.000083 0.000036 0.000037 0.000040 0.000546 0.000040 0.000028 0.000064 0.000134
Golgi 0.002525 0.000774 0.000557 0.002019 0.001807 0.001118 0.000328 0.016352 0.002041 0.000952 0.000435 0.003611
Lipid Particles 0.001099 0.000250 0.000541 0.009514 0.000528 0.000319 0.000638 0.010122 0.000714 0.000286 0.000593 0.009582
Mitochondria 0.000362 0.000231 0.000332 0.000071 0.000224 0.000389 0.000304 0.000060 0.000269 0.000313 0.000317 0.000070
Mitotic Spindle 0.001505 0.000417 0.007615 0.000132 0.000236 0.000608 0.001770 0.001077 0.000649 0.000516 0.004500 0.000237
None 0.015583 0.021991 0.011480 0.003504 0.009507 0.015060 0.009426 0.002652 0.011482 0.018411 0.010385 0.003409
Nuclear Periphery 0.000165 0.000131 0.000262 0.000650 0.000132 0.000162 0.000222 0.000561 0.000143 0.000147 0.000241 0.000640
Nuclear Periphery Foci 0.001094 0.000523 0.000665 0.003618 0.001053 0.000514 0.000325 0.003549 0.001066 0.000518 0.000484 0.003611
Nucleolus 0.000184 0.000250 0.000165 0.000085 0.000060 0.000268 0.000111 0.000103 0.000100 0.000260 0.000136 0.000087
Nucleus 0.147988* 0.239611* 0.193705* 0.205676* 0.172156* 0.279938* 0.371712* 0.098138 0.164299* 0.260444* 0.288559* 0.193728*
Peroxisomes 0.000015 0.000016 0.000033 0.000014 0.000006 0.000008 0.000024 0.000372 0.000009 0.000012 0.000028 0.000053
Vacuole 0.010741 0.003473 0.006119 0.131039 0.005891 0.005309 0.015502 0.151026 0.007468 0.004422 0.011119 0.133260
Vacuole Periphery 0.004754 0.000990 0.000446 0.000734 0.001537 0.003345 0.000470 0.010675 0.002583 0.002207 0.000459 0.001839

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.05 0.29 -3.21 -2.50 -4.04 -2.43 -2.96 -1.69 -1.56 -1.59 -2.04 -2.55 -3.63 -2.78 -3.03
Bud Neck 2.26 -5.26 -5.83 -6.67 -3.10 -0.55 -10.62 -2.30 -2.26 -1.37 1.48 -9.40 -6.75 -6.98 -4.24
Bud Site 3.76 -3.75 0.35 -4.42 4.47 -0.25 -2.02 -1.56 -1.56 -1.51 2.47 -4.92 -1.79 -2.16 -0.70
Cell Periphery 1.45 6.44 -3.31 -3.79 -4.94 1.50 2.19 -2.99 -3.18 -3.27 1.19 5.78 -4.49 -4.72 -5.37
Cytoplasm 8.63 7.52 13.14 9.58 10.33 16.55 34.89 2.69 0.68 -1.67 17.97 29.70 13.64 8.47 4.89
Cytoplasmic Foci 1.29 -3.82 -8.13 -8.40 -7.37 -4.01 -1.41 -3.50 -3.13 -3.39 -2.34 -4.82 -8.88 -8.62 -8.32
Eisosomes 0.47 -0.61 -7.83 -7.85 -7.72 -2.53 -5.47 -3.90 -3.48 -2.83 -1.94 -5.55 -8.84 -8.41 -7.81
Endoplasmic Reticulum 1.09 0.04 2.81 1.85 6.83 0.61 -0.57 -0.60 -0.78 -0.49 1.17 -0.54 3.90 1.92 5.91
Endosome 2.56 -4.17 -2.93 -4.45 -0.34 -0.20 -4.68 -1.42 -1.41 -1.27 1.82 -6.49 -3.34 -3.69 -1.79
Golgi 3.82 4.29 1.49 -3.18 -3.93 2.88 7.71 -1.13 -1.25 -1.36 5.09 8.15 -0.63 -2.10 -2.74
Lipid Particles 3.51 1.29 -5.95 -6.79 -6.33 1.34 -2.15 -3.24 -3.31 -3.05 3.70 -0.95 -6.95 -7.35 -6.82
Mitochondria 1.49 -0.48 4.13 5.13 14.40 -1.88 -4.64 4.98 3.62 8.23 -0.80 -3.63 7.62 5.68 16.53*
Mitotic Spindle 1.30 -5.07 1.61 2.13 7.56 -1.79 -5.60 -1.12 -0.91 0.05 0.41 -7.49 0.74 0.53 8.17
None -2.73 2.15 6.98 13.02 13.56 -4.49 1.08 6.34 8.09 5.86 -5.97 1.93 12.45 16.53 16.93
Nuclear Periphery 0.56 -4.86 -7.15 -7.85 -4.99 -0.85 -13.80 -4.14 -3.57 -1.05 -0.41 -12.71 -8.39 -8.06 -4.92
Nuclear Periphery Foci 3.31 1.46 -5.71 -7.56 -6.66 3.17 4.61 -2.00 -2.59 -2.74 4.69 3.82 -6.44 -7.89 -7.66
Nucleolus -0.50 -0.12 1.53 1.24 6.46 -2.13 -10.54 -1.41 1.74 3.22 -1.95 -3.89 0.20 2.05 7.83
Nucleus -11.41 -8.15 -2.61 2.93 1.05 -15.93 -30.37 2.22 6.84 11.73 -18.85 -26.95 -1.42 5.14 8.32
Peroxisomes 0.03 -1.87 -0.71 -0.51 1.57 -1.02 -1.77 -1.15 -1.14 -0.99 -0.62 -2.55 -1.53 -1.39 -0.35
Vacuole 4.43 1.28 -8.75 -9.20 -8.90 -0.30 -11.75 -2.44 -2.44 -1.89 3.03 -9.74 -9.28 -9.44 -8.48
Vacuole Periphery 2.49 2.97 2.84 0.77 -1.20 -1.57 3.10 -0.90 -0.56 -1.14 0.64 4.07 1.79 1.15 -1.43