Standard name
Human Ortholog
Description Ankyrin-repeat containing, nucleocytoplasmic shuttling chaperone; prevents aggregation of Rps3p in the cytoplasm, associates with nascent Rps3p during its translation in the cytoplasm and delivers it to the 90S in the nucleus; required for 40S ribosomal subunit export, biogenesis and adaptation to osmotic and oxidative stress; expression repressed by heat shock
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.08 0 0 0.07 0.06 0 0.08 0.07 0.08 0.1 0.07 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 1.0 0.98 0.97 0.87 0.9 0.83 0.83 0.86 0.77 0.78 0.77 0.99 0.99 1.0 0.99 0.99 0.99 0.97 0.98 0.98 0.98 0.97 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0.1 0 0 0.05 0.05 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0.07 0.08 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.1 0 0 0.09 0 0.08 0.05 0.08 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 3 2 3 5 1 7 4 3 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 1 0 3 0 2 0 2 2 1 0 1 0 1 0 0 0 0 0 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 23 9 10 23 18 8 32 35 25 37 15 25 3 12 16 8 3 6 0 0 0 0 0 0
Cytoplasm 276 336 255 315 240 266 350 426 277 290 174 147 275 366 356 270 202 197 265 326 246 267 331 278
Endoplasmic Reticulum 3 1 3 4 6 5 41 18 11 18 11 6 3 1 3 3 7 9 1 1 0 1 1 3
Endosome 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Golgi 1 0 1 0 5 0 1 0 0 2 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Mitochondria 0 0 0 0 2 11 2 12 13 26 18 13 0 0 0 1 0 2 0 0 0 0 0 0
Nucleus 1 1 0 0 0 3 6 2 3 0 5 0 0 1 0 0 0 1 0 2 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Nucleolus 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 2 27 8 19 47 14 31 11 15 0 0 0 2 3 1 0 0 0 0 2 1
Unique Cell Count 279 337 259 324 277 295 423 515 322 376 223 191 278 368 357 273 205 199 273 334 252 274 343 298
Labelled Cell Count 306 347 270 345 301 306 455 547 345 413 240 211 281 381 375 285 215 216 273 334 252 274 343 298


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 23.8 21.9 19.3 17.7 18.4 11.2 14.3 13.6 12.2 12.8 11.6 13.3 20.6 19.4 20.3 33.6 32.6 31.1 19.3 20.0 20.2
Std Deviation (1e-4) 7.4 3.8 3.4 2.6 8.1 2.9 2.6 2.5 2.2 2.5 2.7 4.4 4.2 3.1 3.6 8.9 9.6 10.8 3.8 3.7 4.3
Intensity Change (Log2) – – – -0.12 -0.06 -0.79 -0.43 -0.5 -0.65 -0.59 -0.73 -0.53 0.1 0.01 0.08 0.8 0.76 0.69 0.01 0.06 0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.7 1.4 -0.8 2.0 1.6 2.0 2.8 1.4 3.6 -2.1 -0.4 0.4 -0.6 -1.6 -0.5
Cytoplasm -1.0 -5.1 -4.1 -6.3 -6.4 -5.4 -7.6 -7.1 -7.3 0.5 1.3 1.7 0.5 0.1 0.5
Endoplasmic Reticulum 0 0 0 4.4 1.9 1.8 2.5 2.5 0 0 0 0 0 0 2.2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.1 0 0 3.3 4.3 4.7 4.3 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 5.2 0 3.5 5.0 3.4 4.7 3.6 4.6 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.9888 16.6249 14.6112 12.8551 14.7678 15.5231 16.7491 20.3162 19.1285 17.8639 16.0831 18.8882 17.0264 24.5913 23.1298 21.3508 19.9824 22.1977
Actin 0.0387 0.0001 0.0169 0.0607 0.0001 0.0183 0.0157 0.0002 0.0128 0.0005 0.0457 0.0002 0.018 0.0022 0.0006 0.0015 0.0224 0.0003
Bud 0.0006 0 0.0002 0.0008 0 0.0001 0.0002 0.0001 0.0001 0.0001 0.0019 0.0001 0.0003 0.0002 0.0001 0.0001 0.0002 0
Bud Neck 0.0015 0.0001 0.0004 0.0003 0.0002 0.0012 0.0006 0.0001 0.0002 0.0002 0.0007 0.0005 0.0005 0.0005 0.0003 0.0003 0.0006 0.0006
Bud Periphery 0.0006 0 0.0002 0.0008 0 0.0001 0.0002 0 0 0 0.0029 0 0.0003 0 0 0 0.0002 0
Bud Site 0.0039 0 0.0012 0.0015 0 0 0.0007 0.0001 0.0001 0 0.0021 0.0001 0.0007 0.0001 0.0005 0.0001 0.0007 0
Cell Periphery 0.0002 0 0.0001 0 0 0 0.0001 0 0.0001 0 0.0002 0 0.0001 0 0.0001 0 0 0
Cytoplasm 0.8254 0.9973 0.9295 0.8953 0.9964 0.9669 0.9065 0.983 0.9695 0.9547 0.8027 0.9899 0.8115 0.9901 0.9758 0.9898 0.8615 0.995
Cytoplasmic Foci 0.0061 0.0003 0.0148 0.0074 0.0005 0.001 0.0083 0.0029 0.0012 0.0008 0.0266 0.0019 0.026 0.0007 0.003 0.0025 0.04 0.0009
Eisosomes 0.0003 0 0.0002 0.0001 0 0.0003 0.0001 0 0.0001 0 0.0002 0 0.0002 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0092 0.0006 0.0012 0.0009 0.0003 0.0029 0.0131 0.0008 0.0068 0.0055 0.0072 0.0016 0.0127 0.0016 0.0014 0.0014 0.0012 0.0012
Endosome 0.0192 0.0001 0.0029 0.0048 0.0002 0.0017 0.0099 0.0054 0.0011 0.0014 0.0236 0.0015 0.0203 0.0005 0.0026 0.0013 0.0113 0.0004
Golgi 0.0064 0 0.0022 0.0038 0 0.0013 0.0033 0.0006 0.0009 0.0002 0.0064 0.0001 0.0031 0.0001 0.0004 0.0002 0.0036 0.0001
Lipid Particles 0.0043 0 0.0055 0.0037 0 0.0001 0.0064 0.0017 0.0003 0 0.0105 0 0.0038 0 0.0034 0.0002 0.0114 0
Mitochondria 0.0047 0 0.0059 0.0017 0 0.0005 0.0063 0.0009 0.0004 0.0003 0.0251 0.0002 0.0021 0.0001 0.0004 0.0001 0.0006 0
None 0.0138 0.0002 0.0023 0.0013 0.0003 0.0004 0.006 0.0007 0.0003 0.0004 0.0018 0.0009 0.0157 0.0003 0.0014 0.0004 0.0064 0.0002
Nuclear Periphery 0.0307 0.0002 0.0019 0.0025 0.0002 0.0016 0.0065 0.0005 0.0009 0.0011 0.0067 0.0005 0.0184 0.0019 0.0011 0.0003 0.0009 0.0002
Nucleolus 0.0011 0 0.0002 0.0001 0 0 0.0002 0.0001 0 0 0.0003 0 0.0022 0 0.0004 0 0.0002 0
Nucleus 0.0193 0.0007 0.0009 0.0013 0.0016 0.0027 0.0032 0.0015 0.0045 0.0342 0.0258 0.0021 0.0434 0.0013 0.006 0.001 0.0013 0.001
Peroxisomes 0.0011 0 0.0099 0.008 0 0 0.0092 0.0003 0.0001 0 0.0022 0 0.0027 0 0.0005 0.0001 0.0263 0
Punctate Nuclear 0.0087 0 0.0032 0.0043 0 0.0001 0.0022 0.0004 0.0001 0.0001 0.0056 0.0001 0.0154 0.0001 0.0008 0.0001 0.0108 0
Vacuole 0.0027 0.0001 0.0002 0.0004 0.0002 0.0005 0.001 0.0006 0.0005 0.0003 0.0013 0.0003 0.0014 0.0002 0.0009 0.0003 0.0003 0.0001
Vacuole Periphery 0.0015 0 0.0002 0.0003 0 0.0002 0.0005 0.0001 0.0001 0.0001 0.0006 0.0001 0.0012 0.0001 0.0002 0.0001 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 74.1093 67.067 54.6219 62.7173 58.126 69.4961 67.8114 58.6055 57.3957 63.7213
Translational Efficiency 2.6879 3.1066 3.2172 2.6784 2.9526 2.2226 2.3786 3.0715 2.4602 2.5871

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Ankyrin-repeat containing, nucleocytoplasmic shuttling chaperone; prevents aggregation of Rps3p in the cytoplasm, associates with nascent Rps3p during its translation in the cytoplasm and delivers it to the 90S in the nucleus; required for 40S ribosomal subunit export, biogenesis and adaptation to osmotic and oxidative stress; expression repressed by heat shock
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Yar1

Yar1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yar1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available