Standard name
Description Ankyrin-repeat containing, nucleocytoplasmic shuttling chaperone; prevents aggregation of Rps3p in the cytoplasm, associates with nascent Rps3p during its translation in the cytoplasm and delivers it to the 90S in the nucleus; required for 40S ribosomal subunit export, biogenesis and adaptation to osmotic and oxidative stress; expression repressed by heat shock

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.08 0 0 0.07 0.06 0 0.08 0.07 0.08 0.1 0.07 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 1.0 0.98 0.97 0.87 0.9 0.83 0.83 0.86 0.77 0.78 0.77 0.99 0.99 1.0 0.99 0.99 0.99 0.97 0.98 0.98 0.98 0.97 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0.1 0 0 0.05 0.05 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0.07 0.08 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.1 0 0 0.09 0 0.08 0.05 0.08 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 3 2 3 5 1 7 4 3 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 1 0 3 0 2 0 2 2 1 0 1 0 1 0 0 0 0 0 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 23 9 10 23 18 8 32 35 25 37 15 25 3 12 16 8 3 6 0 0 0 0 0 0
Cytoplasm 276 336 255 315 240 266 350 426 277 290 174 147 275 366 356 270 202 197 265 326 246 267 331 278
Endoplasmic Reticulum 3 1 3 4 6 5 41 18 11 18 11 6 3 1 3 3 7 9 1 1 0 1 1 3
Endosome 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Golgi 1 0 1 0 5 0 1 0 0 2 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Mitochondria 0 0 0 0 2 11 2 12 13 26 18 13 0 0 0 1 0 2 0 0 0 0 0 0
Nucleus 1 1 0 0 0 3 6 2 3 0 5 0 0 1 0 0 0 1 0 2 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Nucleolus 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 2 27 8 19 47 14 31 11 15 0 0 0 2 3 1 0 0 0 0 2 1
Unique Cell Count 279 337 259 324 277 295 423 515 322 376 223 191 278 368 357 273 205 199 273 334 252 274 343 298
Labelled Cell Count 306 347 270 345 301 306 455 547 345 413 240 211 281 381 375 285 215 216 273 334 252 274 343 298


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 23.8 21.9 19.3 17.7 18.4 11.2 14.3 13.6 12.2 12.8 11.6 13.3 20.6 19.4 20.3 33.6 32.6 31.1 19.3 20.0 20.2
Std Deviation (1e-4) 7.4 3.8 3.4 2.6 8.1 2.9 2.6 2.5 2.2 2.5 2.7 4.4 4.2 3.1 3.6 8.9 9.6 10.8 3.8 3.7 4.3
Intensity Change (Log2) -0.12 -0.06 -0.79 -0.43 -0.5 -0.65 -0.59 -0.73 -0.53 0.1 0.01 0.08 0.8 0.76 0.69 0.01 0.06 0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.7 1.4 -0.8 2.0 1.6 2.0 2.8 1.4 3.6 -2.1 -0.4 0.4 -0.6 -1.6 -0.5
Cytoplasm -1.0 -5.1 -4.1 -6.3 -6.4 -5.4 -7.6 -7.1 -7.3 0.5 1.3 1.7 0.5 0.1 0.5
Endoplasmic Reticulum 0 0 0 4.4 1.9 1.8 2.5 2.5 0 0 0 0 0 0 2.2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.1 0 0 3.3 4.3 4.7 4.3 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 5.2 0 3.5 5.0 3.4 4.7 3.6 4.6 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yar1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available