Standard name
Human Ortholog
Description Ankyrin-repeat containing, nucleocytoplasmic shuttling chaperone; prevents aggregation of Rps3p in the cytoplasm, associates with nascent Rps3p during its translation in the cytoplasm and delivers it to the 90S in the nucleus; required for 40S ribosomal subunit export, biogenesis and adaptation to osmotic and oxidative stress; expression repressed by heat shock

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.08 0 0 0.07 0.06 0 0.08 0.07 0.08 0.1 0.07 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 1.0 0.98 0.97 0.87 0.9 0.83 0.83 0.86 0.77 0.78 0.77 0.99 0.99 1.0 0.99 0.99 0.99 0.97 0.98 0.98 0.98 0.97 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0.1 0 0 0.05 0.05 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0.07 0.08 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.1 0 0 0.09 0 0.08 0.05 0.08 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 3 2 3 5 1 7 4 3 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 1 0 3 0 2 0 2 2 1 0 1 0 1 0 0 0 0 0 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 23 9 10 23 18 8 32 35 25 37 15 25 3 12 16 8 3 6 0 0 0 0 0 0
Cytoplasm 276 336 255 315 240 266 350 426 277 290 174 147 275 366 356 270 202 197 265 326 246 267 331 278
Endoplasmic Reticulum 3 1 3 4 6 5 41 18 11 18 11 6 3 1 3 3 7 9 1 1 0 1 1 3
Endosome 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Golgi 1 0 1 0 5 0 1 0 0 2 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Mitochondria 0 0 0 0 2 11 2 12 13 26 18 13 0 0 0 1 0 2 0 0 0 0 0 0
Nucleus 1 1 0 0 0 3 6 2 3 0 5 0 0 1 0 0 0 1 0 2 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Nucleolus 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 2 27 8 19 47 14 31 11 15 0 0 0 2 3 1 0 0 0 0 2 1
Unique Cell Count 279 337 259 324 277 295 423 515 322 376 223 191 278 368 357 273 205 199 273 334 252 274 343 298
Labelled Cell Count 306 347 270 345 301 306 455 547 345 413 240 211 281 381 375 285 215 216 273 334 252 274 343 298


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 23.8 21.9 19.3 17.7 18.4 11.2 14.3 13.6 12.2 12.8 11.6 13.3 20.6 19.4 20.3 33.6 32.6 31.1 19.3 20.0 20.2
Std Deviation (1e-4) 7.4 3.8 3.4 2.6 8.1 2.9 2.6 2.5 2.2 2.5 2.7 4.4 4.2 3.1 3.6 8.9 9.6 10.8 3.8 3.7 4.3
Intensity Change (Log2) -0.12 -0.06 -0.79 -0.43 -0.5 -0.65 -0.59 -0.73 -0.53 0.1 0.01 0.08 0.8 0.76 0.69 0.01 0.06 0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.7 1.4 -0.8 2.0 1.6 2.0 2.8 1.4 3.6 -2.1 -0.4 0.4 -0.6 -1.6 -0.5
Cytoplasm -1.0 -5.1 -4.1 -6.3 -6.4 -5.4 -7.6 -7.1 -7.3 0.5 1.3 1.7 0.5 0.1 0.5
Endoplasmic Reticulum 0 0 0 4.4 1.9 1.8 2.5 2.5 0 0 0 0 0 0 2.2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.1 0 0 3.3 4.3 4.7 4.3 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 5.2 0 3.5 5.0 3.4 4.7 3.6 4.6 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.9888 16.6249 14.6112 12.8551 14.7678 15.5231 16.7491 20.3162 19.1285 17.8639 16.0831 18.8882 17.0264 24.5913 23.1298 21.3508 19.9824 22.1977
Actin 0.0387 0.0001 0.0169 0.0607 0.0001 0.0183 0.0157 0.0002 0.0128 0.0005 0.0457 0.0002 0.018 0.0022 0.0006 0.0015 0.0224 0.0003
Bud 0.0006 0 0.0002 0.0008 0 0.0001 0.0002 0.0001 0.0001 0.0001 0.0019 0.0001 0.0003 0.0002 0.0001 0.0001 0.0002 0
Bud Neck 0.0015 0.0001 0.0004 0.0003 0.0002 0.0012 0.0006 0.0001 0.0002 0.0002 0.0007 0.0005 0.0005 0.0005 0.0003 0.0003 0.0006 0.0006
Bud Periphery 0.0006 0 0.0002 0.0008 0 0.0001 0.0002 0 0 0 0.0029 0 0.0003 0 0 0 0.0002 0
Bud Site 0.0039 0 0.0012 0.0015 0 0 0.0007 0.0001 0.0001 0 0.0021 0.0001 0.0007 0.0001 0.0005 0.0001 0.0007 0
Cell Periphery 0.0002 0 0.0001 0 0 0 0.0001 0 0.0001 0 0.0002 0 0.0001 0 0.0001 0 0 0
Cytoplasm 0.8254 0.9973 0.9295 0.8953 0.9964 0.9669 0.9065 0.983 0.9695 0.9547 0.8027 0.9899 0.8115 0.9901 0.9758 0.9898 0.8615 0.995
Cytoplasmic Foci 0.0061 0.0003 0.0148 0.0074 0.0005 0.001 0.0083 0.0029 0.0012 0.0008 0.0266 0.0019 0.026 0.0007 0.003 0.0025 0.04 0.0009
Eisosomes 0.0003 0 0.0002 0.0001 0 0.0003 0.0001 0 0.0001 0 0.0002 0 0.0002 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0092 0.0006 0.0012 0.0009 0.0003 0.0029 0.0131 0.0008 0.0068 0.0055 0.0072 0.0016 0.0127 0.0016 0.0014 0.0014 0.0012 0.0012
Endosome 0.0192 0.0001 0.0029 0.0048 0.0002 0.0017 0.0099 0.0054 0.0011 0.0014 0.0236 0.0015 0.0203 0.0005 0.0026 0.0013 0.0113 0.0004
Golgi 0.0064 0 0.0022 0.0038 0 0.0013 0.0033 0.0006 0.0009 0.0002 0.0064 0.0001 0.0031 0.0001 0.0004 0.0002 0.0036 0.0001
Lipid Particles 0.0043 0 0.0055 0.0037 0 0.0001 0.0064 0.0017 0.0003 0 0.0105 0 0.0038 0 0.0034 0.0002 0.0114 0
Mitochondria 0.0047 0 0.0059 0.0017 0 0.0005 0.0063 0.0009 0.0004 0.0003 0.0251 0.0002 0.0021 0.0001 0.0004 0.0001 0.0006 0
None 0.0138 0.0002 0.0023 0.0013 0.0003 0.0004 0.006 0.0007 0.0003 0.0004 0.0018 0.0009 0.0157 0.0003 0.0014 0.0004 0.0064 0.0002
Nuclear Periphery 0.0307 0.0002 0.0019 0.0025 0.0002 0.0016 0.0065 0.0005 0.0009 0.0011 0.0067 0.0005 0.0184 0.0019 0.0011 0.0003 0.0009 0.0002
Nucleolus 0.0011 0 0.0002 0.0001 0 0 0.0002 0.0001 0 0 0.0003 0 0.0022 0 0.0004 0 0.0002 0
Nucleus 0.0193 0.0007 0.0009 0.0013 0.0016 0.0027 0.0032 0.0015 0.0045 0.0342 0.0258 0.0021 0.0434 0.0013 0.006 0.001 0.0013 0.001
Peroxisomes 0.0011 0 0.0099 0.008 0 0 0.0092 0.0003 0.0001 0 0.0022 0 0.0027 0 0.0005 0.0001 0.0263 0
Punctate Nuclear 0.0087 0 0.0032 0.0043 0 0.0001 0.0022 0.0004 0.0001 0.0001 0.0056 0.0001 0.0154 0.0001 0.0008 0.0001 0.0108 0
Vacuole 0.0027 0.0001 0.0002 0.0004 0.0002 0.0005 0.001 0.0006 0.0005 0.0003 0.0013 0.0003 0.0014 0.0002 0.0009 0.0003 0.0003 0.0001
Vacuole Periphery 0.0015 0 0.0002 0.0003 0 0.0002 0.0005 0.0001 0.0001 0.0001 0.0006 0.0001 0.0012 0.0001 0.0002 0.0001 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 74.1093 67.067 54.6219 62.7173 58.126 69.4961 67.8114 58.6055 57.3957 63.7213
Translational Efficiency 2.6879 3.1066 3.2172 2.6784 2.9526 2.2226 2.3786 3.0715 2.4602 2.5871

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1775 1362 1736 2121 2079 2540 2433 1482 3854 3902 4169 3603

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1293.51 1613.03 1498.73 2137.90 1433.67 1644.45 1887.23 2212.38 1369.12 1633.48 1725.46 2168.54
Standard Deviation 224.09 279.12 285.83 331.75 216.83 263.80 308.70 356.43 231.02 269.66 355.41 344.08
Intensity Change Log 2 0.318482 0.212449 0.724903 0.197892 0.396557 0.625886 0.256346 0.312155 0.673698

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000190 0.000151 0.000075 0.000188 0.000106 0.000118 0.000096 0.000619 0.000145 0.000130 0.000088 0.000365
Bud Neck 0.000311 0.000283 0.001770 0.000644 0.000216 0.000335 0.000268 0.000699 0.000260 0.000316 0.000893 0.000667
Bud Site 0.008251 0.004363 0.003493 0.007872 0.003250 0.005513 0.004527 0.007630 0.005554 0.005111 0.004096 0.007772
Cell Periphery 0.000134 0.000203 0.000113 0.003566 0.000085 0.000083 0.001012 0.002242 0.000108 0.000125 0.000638 0.003022
Cytoplasm 0.916132* 0.950148* 0.674646* 0.906991* 0.934400* 0.946807* 0.949674* 0.911706* 0.925987* 0.947973* 0.835150* 0.908930*
Cytoplasmic Foci 0.038865 0.013376 0.021246 0.006062 0.028905 0.014635 0.004556 0.007303 0.033492 0.014196 0.011506 0.006573
Eisosomes 0.000003 0.000001 0.000016 0.000002 0.000002 0.000001 0.000001 0.000003 0.000002 0.000001 0.000007 0.000002
Endoplasmic Reticulum 0.000799 0.000322 0.001933 0.000347 0.001149 0.000098 0.000275 0.000272 0.000988 0.000176 0.000965 0.000316
Endosome 0.001287 0.000610 0.003897 0.000882 0.000848 0.000488 0.000545 0.001025 0.001050 0.000531 0.001940 0.000941
Golgi 0.004615 0.002339 0.000522 0.006710 0.002559 0.001920 0.004876 0.009545 0.003506 0.002066 0.003063 0.007876
Lipid Particles 0.000725 0.000852 0.001261 0.005942 0.000649 0.000380 0.002815 0.006458 0.000684 0.000545 0.002168 0.006154
Mitochondria 0.000339 0.000054 0.000213 0.000090 0.000099 0.000116 0.000062 0.000083 0.000210 0.000094 0.000125 0.000087
Mitotic Spindle 0.001017 0.000806 0.000662 0.000604 0.000583 0.001008 0.000205 0.001447 0.000783 0.000937 0.000395 0.000951
None 0.002216 0.003374 0.002181 0.000724 0.003530 0.005071 0.000555 0.000643 0.002925 0.004479 0.001232 0.000691
Nuclear Periphery 0.000016 0.000017 0.000183 0.000031 0.000023 0.000010 0.000015 0.000044 0.000020 0.000013 0.000085 0.000036
Nuclear Periphery Foci 0.001196 0.002748 0.001269 0.002215 0.001464 0.000816 0.001324 0.002140 0.001341 0.001491 0.001301 0.002184
Nucleolus 0.000136 0.000075 0.000275 0.000096 0.000064 0.000082 0.000043 0.000076 0.000097 0.000079 0.000140 0.000088
Nucleus 0.009853 0.013735 0.067341 0.005388 0.012586 0.013909 0.003597 0.006012 0.011327 0.013848 0.030140 0.005645
Peroxisomes 0.000189 0.000427 0.000226 0.000100 0.000079 0.000196 0.000061 0.000122 0.000130 0.000276 0.000130 0.000109
Vacuole 0.010885 0.005607 0.216807* 0.047046 0.008484 0.007893 0.022141 0.039234 0.009590 0.007095 0.103201 0.043833
Vacuole Periphery 0.002841 0.000509 0.001872 0.004501 0.000920 0.000521 0.003352 0.002696 0.001805 0.000516 0.002736 0.003759

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.81 2.67 -0.16 -1.33 -5.48 -0.61 0.15 -1.38 -1.34 -1.38 0.59 2.32 -1.46 -1.55 -1.77
Bud Neck -0.15 -10.68 -6.50 -4.87 7.66 -3.02 -2.27 -8.17 -5.91 -7.05 -1.93 -10.60 -10.79 -7.94 2.53
Bud Site 4.41 5.96 0.21 -4.63 -6.63 -3.68 -3.37 -6.49 -2.63 -4.14 0.77 2.79 -4.52 -4.75 -7.34
Cell Periphery -2.17 2.20 -8.79 -8.58 -8.87 0.37 -9.37 -10.46 -10.48 -5.24 -1.42 -9.02 -11.89 -11.81 -9.40
Cytoplasm -8.36 32.84 2.60 11.19 -31.05 -4.52 -4.99 6.24 9.09 9.33 -9.32 22.18 6.67 14.57 -15.61
Cytoplasmic Foci 11.80 7.72 16.40 6.11 13.31 9.57 18.88 15.90 8.02 -4.52 15.11 17.88 22.69 10.32 7.98
Eisosomes 3.42 -9.69 2.95 -1.30 10.88 0.92 3.70 -1.60 -1.75 -2.37 3.72 -8.41 -0.24 -2.23 7.13
Endoplasmic Reticulum 3.17 -1.63 3.31 -0.30 2.53 3.42 2.79 2.72 -1.81 0.07 4.57 0.43 3.68 -2.49 2.42
Endosome 3.31 -8.52 2.30 -1.41 10.36 2.90 2.41 -1.02 -3.00 -2.67 4.68 -5.77 0.95 -3.35 6.22
Golgi 5.69 14.29 -3.31 -6.98 -11.08 2.12 -4.23 -5.43 -5.96 -3.32 5.94 1.25 -6.80 -9.17 -7.01
Lipid Particles -0.94 -6.20 -9.51 -8.90 -8.19 3.67 -7.62 -9.32 -9.75 -5.49 1.57 -8.99 -13.29 -13.51 -8.96
Mitochondria 2.88 1.22 2.52 -2.12 1.21 -0.66 1.99 0.56 1.09 -0.81 2.41 1.68 2.57 0.41 0.77
Mitotic Spindle 0.62 0.60 1.41 0.62 0.56 -1.27 4.29 -1.21 -0.57 -1.77 -0.56 1.97 -0.49 -0.04 -1.51
None -2.08 0.47 3.22 7.19 1.68 -3.01 8.36 8.20 11.94 -0.67 -4.02 4.71 7.66 13.39 1.39
Nuclear Periphery -0.65 -13.92 -5.01 -5.51 12.56 2.26 1.30 -1.34 -2.16 -1.84 2.10 -10.82 -2.38 -3.57 5.32
Nuclear Periphery Foci -4.77 -0.62 -3.87 1.64 -4.05 3.77 0.79 -3.32 -6.82 -4.17 -0.77 0.12 -5.11 -4.51 -5.83
Nucleolus 1.18 -2.24 0.89 -0.85 7.29 -3.56 5.51 -2.41 0.51 -5.74 0.79 -1.44 0.43 -0.95 4.42
Nucleus -4.01 -17.51 4.49 10.98 19.25 -2.09 19.15 5.63 7.04 -4.76 -4.56 -12.30 8.15 13.71 15.84
Peroxisomes -1.12 -1.09 2.77 1.57 3.43 -2.90 2.47 -2.87 1.72 -4.06 -1.86 -0.35 1.24 2.14 1.48
Vacuole 4.32 -35.33 -15.40 -18.94 27.60 0.49 -9.97 -13.55 -13.81 -7.34 3.55 -31.60 -21.02 -23.24 17.31
Vacuole Periphery 4.70 2.25 -2.52 -8.93 -6.09 1.85 -5.29 -4.41 -6.01 1.33 5.04 -2.46 -5.08 -10.83 -2.72