Standard name
Description SET-domain lysine-N-methyltransferase; catalyzes the formation of dimethyllysine residues on the large ribosomal subunit proteins L23 (Rpl23Ap and Rpl23Bp) and monomethyllysine residues on L18 (Rps18Ap and Rps18Bp)

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.29 0.37 0.12 0.23 0.05 0.1 0.08 0.1 0.08 0.1 0.08 0.54 0.46 0.47 0.38 0.19 0.17 0.18 0.23 0.32 0.29
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0 0 0 0 0.08 0.13 0.06 0.26 0.16 0 0 0 0 0 0 0 0 0 0
Nucleus 0.25 0.39 0.71 0.51 0.89 0.73 0.78 0.71 0.77 0.68 0.76 0.32 0.24 0.21 0.17 0.65 0.62 0.51 0.46 0.34 0.41
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0
Vac/Vac Membrane 0.62 0.58 0.53 0.36 0.11 0.21 0.12 0.15 0.14 0.14 0.12 0.21 0.54 0.51 0.59 0.08 0.11 0.21 0.05 0.19 0.16
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 1 1 0 1 0 0 1 0 2 8 0 1 2 6
Bud Neck 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 1 0 0 0 0 3
Bud Site 0 0 0 0 0 0 1 1 0 4 3 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 55 64 2 34 15 28 28 25 31 22 28 73 122 94 81 43 32 7 2 24 39
Endoplasmic Reticulum 1 0 0 0 1 0 0 1 0 2 1 0 15 6 7 0 0 0 0 0 0
Endosome 6 0 0 2 0 2 8 1 2 0 4 2 2 2 7 2 1 0 0 1 1
Golgi 0 0 0 0 1 0 0 0 0 0 0 1 2 2 0 0 0 0 0 0 0
Mitochondria 12 1 0 2 1 11 28 31 25 58 55 0 1 2 0 2 0 0 0 1 0
Nucleus 48 67 12 74 251 207 278 175 298 149 268 43 65 42 36 145 116 21 6 26 56
Nuclear Periphery 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 2 1 3 0 0 0 0 2 0 1 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 2 0 0 1 0 0 1 0 0 0 0 6 3 0 0 0 3
Vac/Vac Membrane 120 100 9 52 30 59 41 37 56 31 43 29 144 101 126 17 19 8 0 15 21
Unique Cell Count 192 173 17 146 281 284 356 247 389 220 353 136 267 200 213 225 188 42 13 79 139
Labelled Cell Count 242 232 23 165 301 307 385 275 416 270 404 148 351 250 259 225 188 42 13 79 139


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.7 7.6 6.6 6.8 7.8 6.5 6.5 6.1 6.1 5.5 5.8 7.4 11.1 11.0 10.1 7.0 7.3
Std Deviation (1e-4) 0.8 1.0 0.7 1.3 1.6 1.4 1.2 1.4 1.1 1.3 1.4 1.5 1.6 1.8 1.8 1.5 1.6
Intensity Change (Log2) 0.06 0.26 -0.01 -0.02 -0.1 -0.09 -0.26 -0.17 0.18 0.76 0.75 0.63 0.09 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 3.3 2.7 2.8 2.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus -1.6 2.3 0.2 0.7 0 0.6 -0.2 0.5 -3.2 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.4 0 0 0 0 0 0 0 0 0.1 -0.2 0.5


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rkm1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available