Standard name
Description Iron-sulfer protein required for synthesis of Wybutosine modified tRNA; Wybutosine is a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions; induction by Yap5p in response to iron provides protection from high iron toxicity; overexpression results in increased cellular iron

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.22 0.27 0.24 0.24 0.13 0.11 0.1 0.12 0.06 0.05 0.07 0.18 0.08 0 0.13 0.13 0.19 0 0.05 0.05 0 0.06 0.06
Bud 0.07 0.05 0.05 0 0 0 0.05 0 0.07 0.09 0.06 0.05 0 0 0 0.05 0.06 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.13 0.28 0.41 0.32 0.32 0.13 0.13 0.13 0.1 0.11 0.08 0.43 0.46 0.35 0.63 0.62 0.66 0.08 0.07 0.16 0.16 0.31 0.29
Cytoplasm 0.32 0.31 0.28 0.39 0.45 0.6 0.62 0.65 0.7 0.65 0.72 0.37 0.47 0.63 0.37 0.31 0.25 0 0.07 0.09 0 0 0
Endoplasmic Reticulum 0.38 0.44 0.23 0.24 0.28 0.22 0.22 0.21 0.12 0.07 0.12 0.37 0.3 0.25 0 0 0 0.77 0.69 0.5 0.66 0.53 0.49
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.08 0.05 0 0.11 0.17 0.08 0 0 0 0 0 0 0 0 0.09 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 15 37 47 10 25 18 34 27 14 10 10 11 17 7 16 14 19 4 8 12 3 14 16
Bud 5 7 9 1 8 7 18 9 15 17 9 3 5 3 3 6 6 0 1 1 1 0 1
Bud Neck 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 3 4 8 2 3 8
Bud Site 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 9 39 80 13 60 20 46 29 22 21 12 26 100 64 78 69 67 8 11 43 16 71 77
Cytoplasm 22 43 54 16 86 95 213 149 158 121 103 22 103 117 46 34 26 1 11 22 2 3 11
Endoplasmic Reticulum 26 60 45 10 54 34 76 48 26 13 17 22 66 46 1 1 3 83 116 135 66 121 130
Endosome 0 0 0 0 0 0 1 0 0 0 1 0 1 1 0 1 1 0 1 0 0 0 1
Golgi 0 0 0 0 0 0 0 1 0 0 0 0 0 0 2 1 3 0 2 0 0 2 1
Mitochondria 0 1 0 1 0 12 17 10 24 32 12 1 1 1 2 1 2 0 3 25 1 6 4
Nucleus 0 0 0 0 0 0 0 2 0 1 1 0 0 1 0 1 0 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 1 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 1 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 1 5 5 4 2 3 1 0 3 0 1 1 1 1 4 17 2 4 7
Unique Cell Count 68 137 194 41 190 158 342 231 225 185 144 60 218 185 124 111 102 109 168 269 101 231 264
Labelled Cell Count 78 187 236 51 235 192 410 281 262 220 166 85 298 240 149 129 128 109 168 269 101 231 264


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.4 10.2 8.5 8.1 8.7 6.2 6.7 6.7 5.8 5.3 6.0 9.0 8.6 7.9 16.7 17.0 16.5 9.4 8.8 8.6
Std Deviation (1e-4) 1.5 1.5 0.9 0.9 1.1 0.9 1.0 1.0 0.8 0.7 1.0 1.3 1.1 1.4 3.2 2.8 3.1 1.2 1.4 1.3
Intensity Change (Log2) -0.06 0.04 -0.45 -0.33 -0.34 -0.54 -0.67 -0.51 0.09 0.02 -0.11 0.97 1.01 0.96 0.15 0.05 0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 -2.8 -3.1 -4.4 -3.4 -5.2 -5.1 -4.2 -1.0 -4.6 -5.7 -2.5 -2.4 -1.1
Bud 0 -0.2 -0.1 0.3 -0.4 0.9 1.8 0.7 0 -1.3 -1.7 0 0.3 0.5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.1 -2.0 -5.9 -7.3 -6.7 -7.5 -6.6 -6.7 0.3 0.9 -1.3 3.8 3.5 4.0
Cytoplasm 1.4 3.5 6.1 7.7 7.5 8.7 7.3 8.0 1.3 4.0 6.9 1.7 0.5 -0.4
Endoplasmic Reticulum 0.2 1.2 -0.4 -0.3 -0.6 -3.2 -4.4 -2.7 2.1 1.6 0.4 -5.5 -5.2 -4.5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.9 3.2 0 4.7 6.1 4.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tyw1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available