Standard name
Human Ortholog
Description Iron-sulfer protein required for synthesis of Wybutosine modified tRNA; Wybutosine is a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions; induction by Yap5p in response to iron provides protection from high iron toxicity; overexpression results in increased cellular iron

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.22 0.27 0.24 0.24 0.13 0.11 0.1 0.12 0.06 0.05 0.07 0.18 0.08 0 0.13 0.13 0.19 0 0.05 0.05 0 0.06 0.06
Bud 0.07 0.05 0.05 0 0 0 0.05 0 0.07 0.09 0.06 0.05 0 0 0 0.05 0.06 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.13 0.28 0.41 0.32 0.32 0.13 0.13 0.13 0.1 0.11 0.08 0.43 0.46 0.35 0.63 0.62 0.66 0.08 0.07 0.16 0.16 0.31 0.29
Cytoplasm 0.32 0.31 0.28 0.39 0.45 0.6 0.62 0.65 0.7 0.65 0.72 0.37 0.47 0.63 0.37 0.31 0.25 0 0.07 0.09 0 0 0
Endoplasmic Reticulum 0.38 0.44 0.23 0.24 0.28 0.22 0.22 0.21 0.12 0.07 0.12 0.37 0.3 0.25 0 0 0 0.77 0.69 0.5 0.66 0.53 0.49
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.08 0.05 0 0.11 0.17 0.08 0 0 0 0 0 0 0 0 0.09 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 15 37 47 10 25 18 34 27 14 10 10 11 17 7 16 14 19 4 8 12 3 14 16
Bud 5 7 9 1 8 7 18 9 15 17 9 3 5 3 3 6 6 0 1 1 1 0 1
Bud Neck 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 3 4 8 2 3 8
Bud Site 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 9 39 80 13 60 20 46 29 22 21 12 26 100 64 78 69 67 8 11 43 16 71 77
Cytoplasm 22 43 54 16 86 95 213 149 158 121 103 22 103 117 46 34 26 1 11 22 2 3 11
Endoplasmic Reticulum 26 60 45 10 54 34 76 48 26 13 17 22 66 46 1 1 3 83 116 135 66 121 130
Endosome 0 0 0 0 0 0 1 0 0 0 1 0 1 1 0 1 1 0 1 0 0 0 1
Golgi 0 0 0 0 0 0 0 1 0 0 0 0 0 0 2 1 3 0 2 0 0 2 1
Mitochondria 0 1 0 1 0 12 17 10 24 32 12 1 1 1 2 1 2 0 3 25 1 6 4
Nucleus 0 0 0 0 0 0 0 2 0 1 1 0 0 1 0 1 0 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 1 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 1 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 1 5 5 4 2 3 1 0 3 0 1 1 1 1 4 17 2 4 7
Unique Cell Count 68 137 194 41 190 158 342 231 225 185 144 60 218 185 124 111 102 109 168 269 101 231 264
Labelled Cell Count 78 187 236 51 235 192 410 281 262 220 166 85 298 240 149 129 128 109 168 269 101 231 264


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.4 10.2 8.5 8.1 8.7 6.2 6.7 6.7 5.8 5.3 6.0 9.0 8.6 7.9 16.7 17.0 16.5 9.4 8.8 8.6
Std Deviation (1e-4) 1.5 1.5 0.9 0.9 1.1 0.9 1.0 1.0 0.8 0.7 1.0 1.3 1.1 1.4 3.2 2.8 3.1 1.2 1.4 1.3
Intensity Change (Log2) -0.06 0.04 -0.45 -0.33 -0.34 -0.54 -0.67 -0.51 0.09 0.02 -0.11 0.97 1.01 0.96 0.15 0.05 0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 -2.8 -3.1 -4.4 -3.4 -5.2 -5.1 -4.2 -1.0 -4.6 -5.7 -2.5 -2.4 -1.1
Bud 0 -0.2 -0.1 0.3 -0.4 0.9 1.8 0.7 0 -1.3 -1.7 0 0.3 0.5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.1 -2.0 -5.9 -7.3 -6.7 -7.5 -6.6 -6.7 0.3 0.9 -1.3 3.8 3.5 4.0
Cytoplasm 1.4 3.5 6.1 7.7 7.5 8.7 7.3 8.0 1.3 4.0 6.9 1.7 0.5 -0.4
Endoplasmic Reticulum 0.2 1.2 -0.4 -0.3 -0.6 -3.2 -4.4 -2.7 2.1 1.6 0.4 -5.5 -5.2 -4.5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.9 3.2 0 4.7 6.1 4.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.8583 10.6085 9.5274 8.8093 9.0063 9.8757 8.4472 9.1668 8.7358 7.46 8.138 8.6846 6.9336 7.0946 6.7123 7.0325 6.2607 7.0412
Actin 0.07 0.0051 0.0287 0.0078 0.0202 0.019 0.012 0.0062 0.0123 0.0391 0.0173 0.0119 0.0187 0.0034 0.0114 0.0025 0.0229 0.0102
Bud 0.0013 0.0004 0.0025 0.0005 0.0004 0.0005 0.0013 0.0005 0.0011 0.0005 0.0008 0.0002 0.0005 0.0002 0.0008 0.0002 0.0003 0.0003
Bud Neck 0.0024 0.0037 0.0073 0.0202 0.0148 0.023 0.0016 0.0025 0.003 0.0251 0.0102 0.0144 0.0021 0.0042 0.0051 0.0031 0.0111 0.0207
Bud Periphery 0.0099 0.002 0.0186 0.0079 0.0032 0.0047 0.0087 0.004 0.0085 0.0033 0.006 0.002 0.0024 0.0011 0.0066 0.004 0.0031 0.0031
Bud Site 0.0068 0.0375 0.0128 0.0038 0.0026 0.0017 0.0017 0.013 0.0093 0.0009 0.0013 0.0002 0.0026 0.0162 0.0116 0.0003 0.0003 0.0008
Cell Periphery 0.0913 0.1463 0.1653 0.1195 0.1272 0.0738 0.0775 0.162 0.0998 0.0519 0.0992 0.059 0.0764 0.1066 0.1068 0.0781 0.0659 0.0809
Cytoplasm 0.1316 0.189 0.1497 0.0649 0.1446 0.1512 0.1661 0.2744 0.1217 0.141 0.1523 0.0946 0.1592 0.1819 0.1851 0.0871 0.1373 0.1774
Cytoplasmic Foci 0.0157 0.0028 0.0072 0.017 0.0166 0.0092 0.01 0.0032 0.0058 0.0101 0.0092 0.0035 0.0085 0.0022 0.0057 0.0087 0.0072 0.004
Eisosomes 0.0031 0.0012 0.0016 0.0012 0.0017 0.0015 0.0013 0.0015 0.0019 0.005 0.0019 0.0009 0.002 0.0009 0.0013 0.0004 0.008 0.0014
Endoplasmic Reticulum 0.5145 0.5462 0.5164 0.6257 0.5717 0.6478 0.6082 0.4858 0.6613 0.6189 0.5842 0.7653 0.6438 0.6297 0.5976 0.7359 0.6317 0.6555
Endosome 0.0228 0.0021 0.0109 0.0294 0.0132 0.0086 0.0199 0.0034 0.0078 0.0314 0.0233 0.0061 0.0133 0.0025 0.0108 0.0057 0.0123 0.0086
Golgi 0.0209 0.0033 0.0122 0.0148 0.0288 0.0142 0.021 0.0121 0.0182 0.0176 0.0324 0.0123 0.0086 0.0039 0.0093 0.0067 0.0205 0.009
Lipid Particles 0.055 0.0322 0.0309 0.0573 0.0247 0.0185 0.0299 0.0191 0.0151 0.0108 0.0083 0.0162 0.0156 0.0283 0.0149 0.0296 0.0182 0.0137
Mitochondria 0.0153 0.0002 0.0023 0.0011 0.0059 0.004 0.0045 0.0004 0.0111 0.0014 0.0402 0.0035 0.0047 0.0002 0.0057 0.002 0.0503 0.0028
None 0.018 0.002 0.0064 0.0008 0.0014 0.0009 0.0062 0.0006 0.0074 0.0143 0.0007 0.0008 0.0051 0.001 0.0041 0.0006 0.0022 0.0012
Nuclear Periphery 0.0057 0.0059 0.0057 0.0074 0.004 0.0033 0.0082 0.0032 0.0051 0.0057 0.0031 0.0025 0.0145 0.0068 0.009 0.0039 0.0033 0.0034
Nucleolus 0.0002 0.0001 0.0003 0.0004 0.0001 0.0001 0.0007 0.0001 0.0002 0.0007 0.0001 0.0001 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001
Nucleus 0.0008 0.0008 0.001 0.0008 0.0005 0.0008 0.0016 0.0005 0.0007 0.002 0.0007 0.0004 0.0028 0.0022 0.0012 0.0005 0.0005 0.0007
Peroxisomes 0.0024 0.0001 0.0016 0.0042 0.0065 0.0045 0.0063 0 0.0016 0.0009 0.0002 0.0001 0.0045 0 0.002 0.0231 0.0015 0.0001
Punctate Nuclear 0.001 0.0003 0.0004 0.0005 0.0003 0.0002 0.0025 0.0001 0.0003 0.0077 0.0002 0.0001 0.0018 0.0003 0.0019 0.0002 0.0003 0.0002
Vacuole 0.0104 0.0186 0.0175 0.0138 0.011 0.0117 0.0095 0.0071 0.007 0.0109 0.0072 0.0054 0.0093 0.008 0.0078 0.0071 0.0027 0.0054
Vacuole Periphery 0.0008 0.0002 0.0008 0.0009 0.0006 0.0006 0.0013 0.0002 0.0007 0.0008 0.0011 0.0004 0.0031 0.0002 0.001 0.0003 0.0003 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 33.1067 35.2712 38.507 34.4494 39.6902 29.4622 32.6907 35.2748 31.2663 37.3964
Translational Efficiency 1.2031 0.9919 0.7361 0.7829 0.7184 0.8117 0.836 0.5002 0.5254 0.5979

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2292 371 1089 1659 1620 1655 87 2570 3912 2026 1176 4229

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 889.98 1157.96 1279.20 1520.80 986.47 1054.86 1434.33 1511.89 929.94 1073.74 1290.68 1515.39
Standard Deviation 108.52 165.01 169.68 189.73 134.44 165.65 182.55 208.29 129.01 170.27 175.43 201.26
Intensity Change Log 2 0.379741 0.523397 0.772986 0.096704 0.540030 0.616006 0.237880 0.532165 0.692592

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.005203 0.010459 0.020877 0.013381 0.006026 0.009536 0.023287 0.006848 0.005544 0.009705 0.021055 0.009411
Bud Neck 0.020875 0.015181 0.019462 0.007904 0.010806 0.010124 0.018506 0.005868 0.016705 0.011050 0.019391 0.006667
Bud Site 0.043606 0.066708 0.069958 0.050737 0.036378 0.045890 0.053767 0.031150 0.040613 0.049702 0.068761 0.038834
Cell Periphery 0.050879 0.175447* 0.022932 0.157278* 0.063618 0.137005* 0.117072* 0.212101* 0.056154 0.144044* 0.029896 0.190594*
Cytoplasm 0.350698* 0.151165* 0.193614* 0.081862 0.304779* 0.211574* 0.048593 0.110664* 0.331682* 0.200512* 0.182885* 0.099365
Cytoplasmic Foci 0.045714 0.030005 0.022737 0.008647 0.034315 0.030799 0.007495 0.006895 0.040993 0.030653 0.021609 0.007582
Eisosomes 0.009719 0.006120 0.001893 0.002282 0.008467 0.007288 0.004271 0.002387 0.009200 0.007074 0.002069 0.002346
Endoplasmic Reticulum 0.283158* 0.421529* 0.416496* 0.476676* 0.407488* 0.399355* 0.611973* 0.428180* 0.334644* 0.403416* 0.430957* 0.447204*
Endosome 0.009247 0.012640 0.016029 0.008657 0.006950 0.008333 0.009746 0.005363 0.008296 0.009122 0.015564 0.006656
Golgi 0.022343 0.014016 0.004131 0.007174 0.016510 0.013725 0.003829 0.004745 0.019927 0.013778 0.004108 0.005698
Lipid Particles 0.035997 0.036738 0.041490 0.129380* 0.029517 0.041083 0.034715 0.106460* 0.033314 0.040287 0.040989 0.115451*
Mitochondria 0.030537 0.003369 0.000633 0.002499 0.011057 0.009617 0.000255 0.004716 0.022470 0.008473 0.000605 0.003846
Mitotic Spindle 0.000249 0.000460 0.003546 0.002018 0.000115 0.000363 0.000354 0.000826 0.000193 0.000381 0.003310 0.001294
None 0.002174 0.000048 0.001460 0.000553 0.001430 0.000713 0.000049 0.001351 0.001866 0.000591 0.001356 0.001038
Nuclear Periphery 0.000213 0.000310 0.001411 0.001531 0.000198 0.000344 0.000445 0.002660 0.000207 0.000338 0.001339 0.002217
Nuclear Periphery Foci 0.000554 0.001255 0.027699 0.003696 0.000852 0.000898 0.010212 0.002244 0.000677 0.000963 0.026406 0.002814
Nucleolus 0.000196 0.000052 0.000079 0.000066 0.000092 0.000113 0.000014 0.000124 0.000153 0.000102 0.000074 0.000101
Nucleus 0.006121 0.001889 0.034822 0.003059 0.004020 0.002033 0.003667 0.002928 0.005251 0.002007 0.032517 0.002979
Peroxisomes 0.066224 0.024993 0.000753 0.010518 0.045101 0.060725 0.001206 0.032666 0.057477 0.054182 0.000786 0.023978
Vacuole 0.012173 0.023606 0.099402 0.029638 0.009948 0.008027 0.050238 0.029191 0.011252 0.010880 0.095765 0.029367
Vacuole Periphery 0.004122 0.004009 0.000578 0.002446 0.002333 0.002455 0.000304 0.002633 0.003381 0.002740 0.000558 0.002559

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.09 -9.08 -6.53 -1.53 3.58 -3.27 -2.41 -1.26 2.32 2.28 -4.72 -9.25 -6.08 0.11 6.61
Bud Neck 2.17 -0.15 8.88 2.94 9.98 0.59 -1.76 5.12 4.71 2.86 5.01 -3.09 10.88 5.02 12.20
Bud Site -3.21 -6.10 -2.17 2.00 3.96 -2.67 -1.68 1.63 4.23 2.12 -2.99 -7.19 0.48 3.30 7.39
Cell Periphery -10.23 11.22 -19.30 1.50 -25.58* -12.94 -2.60 -27.54* -11.51 -4.85 -18.26 9.91 -35.27 -8.18 -40.62*
Cytoplasm 18.61 19.13 43.46 6.65 14.85 11.53 21.26 27.75 16.12 -5.58 21.64 20.30 50.34 18.94 12.54
Cytoplasmic Foci 3.86 10.86 22.01 5.48 8.94 1.51 7.05 15.46 14.06 0.46 5.60 10.69 27.54 14.88 10.24
Eisosomes 3.95 17.88 15.92 4.59 -1.80 1.41 3.83 8.28 11.35 2.21 3.96 17.35 17.07 12.23 -1.90
Endoplasmic Reticulum -7.30 -9.08 -18.03 -2.59 -6.32 0.73 -4.89 -1.89 -2.70 4.42 -7.81 -6.36 -15.33 -4.65 -3.62
Endosome -1.91 -7.56 0.65 2.13 7.52 -2.26 -1.26 3.68 5.37 1.95 -1.38 -9.08 3.85 4.15 11.18
Golgi 4.22 18.30 11.20 3.46 -3.26 2.23 13.02 11.10 8.04 -1.18 5.88 22.10 16.60 8.31 -2.96
Lipid Particles -0.18 -2.85 -18.74 -14.62 -16.12 -5.22 -1.02 -21.24 -16.79 -9.48 -3.71 -4.08 -27.93 -22.82 -20.56
Mitochondria 19.98* 23.36 21.16 1.48 -5.27 1.21 11.48 6.46 6.25 -14.14 13.37 25.45 21.01 7.19 -13.91
Mitotic Spindle -0.82 -3.82 -3.66 -2.97 1.86 -2.26 -3.34 -7.67 -3.18 -3.92 -1.77 -3.88 -5.43 -4.13 2.66
None 7.77 1.11 5.58 -5.35 1.25 1.76 4.04 0.18 -1.74 -4.44 4.59 0.90 2.95 -1.75 0.39
Nuclear Periphery -2.28 -7.96 -13.22 -11.28 0.19 -8.16 -3.49 -19.29 -18.07 -14.87 -8.54 -8.10 -23.05 -21.29 -4.06
Nuclear Periphery Foci -5.45 -17.90 -8.58 -6.40 15.41 -0.59 -3.54 -5.62 -5.56 3.04 -5.45 -18.09 -10.32 -8.92 16.43
Nucleolus 13.89* 11.15 13.16 -1.81 2.27 -0.72 12.61 -4.44 -0.07 -18.08* 1.37 9.85 7.91 0.21 -3.49
Nucleus 9.95 -15.89 6.96 -3.12 17.12 7.45 -0.16 3.48 -4.58 1.19 15.29 -16.14 8.96 -5.59 17.22
Peroxisomes 12.65 28.29* 22.66 5.95 -11.46 -4.71 19.83 4.80 9.82 -21.41* 1.24 34.56* 17.77 13.17 -24.41*
Vacuole -3.33 -23.18 -11.77 -1.89 18.22 1.96 -4.01 -14.73 -18.01 2.23 0.30 -23.70 -18.58 -16.56 18.90
Vacuole Periphery 0.11 12.80 3.01 1.46 -3.78 -0.45 10.47 -0.76 -0.46 -5.94 2.15 15.04 2.30 0.43 -6.47