Standard name
Description Conserved casein kinase; regulates diverse events including: vesicular traffic, DNA repair, the CVT pathway, monopolar attachment of sister kinetochores at meiosis I, and ribosomal subunit biogenesis; monopolin subunit; binds the RNAPII CTD; phosphorylates COPII coat subunits; interacts with Sit4p phosphatase; antagonizes calcineurin signaling, reducing nuclear accumulation of Crz1p; phosphorylates Dsn1p, the kinetochore receptor for monopolin; homolog of mammalian CK1delta

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0.05 0 0 0 0.08 0 0.08 0 0 0 0.05 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.05 0 0.06 0 0.08 0.07 0 0 0 0 0 0 0.06 0.11 0 0.08 0.09 0.09
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.71 0.75 0.68 0.34 0.38 0.59 0.5 0.5 0.44 0.5 0.48 0.54 0.52 0.63 0.6 0.79 0.65 0.78 0.41 0.37 0.5 0.5 0.36 0.41
Endoplasmic Reticulum 0.1 0.19 0.05 0 0.08 0.22 0.13 0.13 0.07 0.18 0.11 0.15 0.12 0.05 0.11 0 0 0 0.06 0 0 0.06 0 0.05
Endosome 0 0 0.05 0.05 0.08 0 0.12 0.1 0.09 0.06 0 0 0 0 0 0 0.07 0 0.08 0 0 0 0.07 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.06 0 0 0.05 0.1 0 0.09 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.1 0 0.06 0.16 0.07 0 0 0 0.06 0 0.06 0 0.07 0.08 0.07 0.06 0 0 0.14 0.22 0.17 0.08 0.15 0.12
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0
Vac/Vac Membrane 0.18 0.06 0.17 0.46 0.32 0.06 0.18 0.16 0.21 0.13 0.15 0.07 0.35 0.28 0.26 0.11 0.3 0.15 0.08 0.1 0.05 0.12 0.17 0.16
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 0 1 4 7 10 8 13 11 23 7 21 5 2 0 6 3 3 4 2 1 1 4 3
Bud 0 0 0 0 8 8 18 10 15 13 20 19 2 3 0 3 1 1 10 7 3 5 19 30
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 1 4 2 7 7
Bud Site 0 0 0 0 0 0 1 0 2 2 2 1 0 0 0 0 0 0
Cell Periphery 0 0 0 0 1 1 2 2 9 6 9 16 0 2 5 3 5 4 0 0 0 0 0 0
Cytoplasm 104 12 44 31 71 126 191 156 113 153 120 145 100 151 161 105 86 98 78 25 52 34 76 136
Endoplasmic Reticulum 15 3 3 2 15 47 49 40 17 55 27 40 23 12 30 1 5 5 11 1 3 4 7 17
Endosome 1 0 3 5 15 9 46 30 23 19 10 9 2 1 6 3 9 3 15 3 4 1 15 16
Golgi 0 0 0 0 0 0 0 3 0 1 0 0 0 0 1 1 1 0 0 0 0 0 0 1
Mitochondria 0 0 2 2 11 5 14 15 25 12 23 27 0 0 0 1 0 0 0 1 1 0 1 4
Nucleus 14 0 4 15 13 8 7 3 16 7 16 12 13 19 19 8 1 3 26 15 18 5 31 40
Nuclear Periphery 0 0 0 0 0 0 0 1 1 0 1 0 0 0 0 0 0 0 7 1 0 0 3 7
Nucleolus 0 0 4 0 4 4 17 8 3 4 3 9 0 0 0 0 0 0 1 0 2 0 0 2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 8 0 6 0 3 3
Vac/Vac Membrane 27 1 11 42 61 12 68 50 55 40 36 19 67 68 70 15 39 19 14 6 4 8 37 51
Unique Cell Count 146 16 65 92 188 212 379 310 256 303 248 271 191 241 270 133 132 126 192 68 107 69 214 332
Labelled Cell Count 164 16 72 101 206 230 421 331 290 335 274 318 212 258 292 146 151 136 192 68 107 69 214 332


Ambiguous, Bud, Bud Neck, Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.6 12.9 9.1 9.6 9.2 8.6 8.6 8.9 9.1 9.3 9.2 9.5 11.9 11.0 12.1 11.5 12.1 12.4 14.7 15.5
Std Deviation (1e-4) 2.1 1.6 1.6 1.6 1.7 1.3 1.4 1.5 1.7 1.8 1.8 1.8 1.4 1.7 1.6 2.1 2.0 2.2 2.4 2.8
Intensity Change (Log2) 0.08 0.02 -0.07 -0.07 -0.03 0.0 0.04 0.03 0.08 0.4 0.27 0.41 0.34 0.42 0.45 0.7 0.78


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -4.2 -4.2 -1.2 -2.6 -2.6 -3.4 -2.5 -2.8 -2.1 -2.2 -0.7 -1.2 1.7 -0.4 1.5
Endoplasmic Reticulum 0 0 3.2 1.9 1.9 0 2.7 1.5 2.2 1.7 0 1.6 0 0 0
Endosome 0 0 0 1.8 1.3 1.2 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 1.7 0 1.6 1.8 0 0 0 0 0 0
Nucleus 1.9 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 3.7 2.4 -2.9 0.2 -0.2 0.8 -0.8 -0.5 -2.5 2.7 1.8 1.5 -1.1 1.9 -0.3


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hrr25-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available