Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L7B; required for processing of 27SA3 pre-rRNA to 27SB pre-rRNA during assembly of large ribosomal subunit; depletion leads to a turnover of pre-rRNA; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); binds to Domain II of 25S and 5.8S rRNAs; homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7B has a paralog, RPL7A, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0.07 0.08 0.18 0.15 0.1 0.15 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.08 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.92 0.87 0.83 0.44 0.61 0.51 0.35 0.3 0.32 0.27 0.26 0.33 0.58 0.5 0.56 0.43 0.48 0.59 0.83 0.76 0.68 0.75 0.7 0.42
Endoplasmic Reticulum 0 0 0 0.11 0.09 0.09 0.12 0.14 0.11 0.05 0.1 0.1 0 0 0 0 0.07 0.07 0 0 0.07 0 0 0.09
Endosome 0 0 0 0 0 0 0 0 0 0.09 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.09
Golgi 0 0 0 0.25 0.22 0.27 0.38 0.26 0.13 0.13 0.23 0.08 0.11 0.14 0.15 0.21 0.18 0.09 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.18 0.29 0.15 0.25 0 0 0 0.08 0 0.05 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.05 0.06 0.09 0.2 0.12 0.14 0.14 0.22 0.21 0.21 0.18 0.17 0.3 0.33 0.23 0.32 0.24 0.21 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.05 0.08
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 0 0 0.07 0 0 0 0 0.11 0.15 0.09 0 0 0 0 0.05 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 2 3 14 19 25 26 16 31 0 1 0 2 0 0 0 1 0 1 2 5
Bud 2 4 4 2 1 3 5 8 11 10 10 9 1 2 1 2 0 2 2 9 1 1 6 11
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 1
Bud Site 0 0 0 0 0 0 0 0 2 5 1 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 5 0 0 0 1 0 3 1 3 7 6 1 0 2 1 3 0 0 1 0 0 0 0
Cytoplasm 167 344 146 41 107 87 67 72 44 46 40 70 123 146 132 163 116 112 147 353 131 128 170 95
Endoplasmic Reticulum 4 13 7 10 15 15 23 35 15 9 15 21 6 12 9 14 16 13 2 8 13 4 9 20
Endosome 0 2 0 0 0 1 1 5 2 16 6 10 0 0 0 3 1 0 0 7 6 4 5 21
Golgi 2 3 5 23 39 46 72 63 18 22 35 17 24 42 35 82 44 17 0 2 3 2 5 4
Mitochondria 0 0 1 4 1 4 3 8 25 49 24 53 7 9 3 30 5 10 0 0 2 0 0 1
Nucleus 0 1 0 0 1 0 0 0 0 0 0 0 0 1 0 4 8 8 3 15 1 2 1 3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 2 7 1 0 2 3
Nucleolus 9 23 16 19 21 24 26 53 29 36 28 36 63 95 54 122 59 40 2 12 5 0 3 7
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 3 3
SpindlePole 0 0 0 0 0 0 0 1 2 7 3 3 0 0 0 4 0 0 3 10 9 10 11 18
Vac/Vac Membrane 4 9 4 1 1 2 5 13 4 2 11 9 3 10 9 42 36 17 3 18 2 6 11 15
Unique Cell Count 182 394 175 93 174 172 190 243 139 170 155 212 211 291 234 382 243 189 178 463 192 172 245 226
Labelled Cell Count 188 404 183 100 188 186 216 280 178 231 197 268 228 318 245 469 288 219 178 463 192 172 245 226


Cytoplasm, Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 84.7 100.5 87.8 90.4 69.9 50.6 41.2 35.4 30.1 23.9 26.6 24.7 87.8 81.0 77.8 75.3 89.7 95.1 83.8 84.6 88.3
Std Deviation (1e-4) 19.2 24.3 19.9 17.7 16.1 11.7 9.7 10.4 8.5 7.4 7.8 8.9 22.6 23.6 22.2 39.2 45.9 49.9 21.5 22.6 29.3
Intensity Change (Log2) – – – 0.04 -0.33 -0.79 -1.09 -1.31 -1.54 -1.88 -1.72 -1.83 0.0 -0.12 -0.17 -0.22 0.03 0.11 -0.07 -0.05 0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 3.7 3.8 5.8 5.4 4.4 5.3 0 0 0 0 0 0
Bud 0 0 0 0 0.6 2.3 1.7 1.9 1.1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -6.7 -4.6 -6.5 -9.3 -10.9 -9.3 -10.5 -10.5 -9.9 -5.3 -7.2 -5.8 -9.0 -7.4 -5.1
Endoplasmic Reticulum 2.2 1.8 1.8 2.8 3.5 2.3 0.6 2.1 2.2 -0.6 0.1 -0.1 -0.2 1.1 1.2
Endosome 0 0 0 0 0 0 4.2 0 0 0 0 0 0 0 0
Golgi 5.6 5.5 6.3 8.2 6.3 3.4 3.5 5.5 2.2 3.2 4.0 4.1 5.6 4.8 2.5
Mitochondria 0 0 0 0 0 5.6 7.5 5.1 6.9 0 0 0 3.5 0 2.6
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 2.6 0.9 1.4 1.4 3.4 2.9 3.1 2.4 2.3 5.0 5.8 3.7 5.8 4.0 3.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 1.6 0 0 2.1 1.1 0 0.7 0.9 3.5 4.3 2.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 84.2453 117.3156 103.4556 93.9413 95.1592 92.5185 81.8717 107.2203 96.443 94.2834 83.1488 90.5963 79.0364 129.591 117.4724 110.6263 117.0168 116.1612
Actin 0.0019 0.0006 0.0005 0.0013 0.0007 0.0003 0.0314 0.0017 0.0036 0.0009 0.0006 0.0022 0.062 0.0003 0.0125 0.0002 0.0002 0.001
Bud 0.0018 0.001 0.0014 0.0014 0.0003 0.0006 0.0021 0.0017 0.0019 0.0006 0.0002 0.0007 0.0013 0.001 0.0049 0.0008 0.0002 0.0005
Bud Neck 0.0011 0.0018 0.0009 0.0008 0.0007 0.0006 0.0013 0.0012 0.0055 0.0005 0.0004 0.0005 0.001 0.0008 0.0007 0.0006 0.0003 0.0008
Bud Periphery 0.0018 0.0006 0.0005 0.001 0.0002 0.0003 0.0012 0.0016 0.0013 0.0003 0.0001 0.0001 0.001 0.0003 0.0011 0.0004 0.0001 0.0002
Bud Site 0.0024 0.0024 0.0014 0.0014 0.0002 0.0002 0.0048 0.0052 0.0082 0.0006 0.0002 0.0003 0.004 0.0019 0.0029 0.0004 0.0001 0.0003
Cell Periphery 0.0124 0.0107 0.0152 0.0088 0.003 0.0027 0.0037 0.004 0.004 0.0046 0.0011 0.0006 0.0043 0.0074 0.0091 0.008 0.0019 0.0018
Cytoplasm 0.3657 0.4179 0.2463 0.3426 0.341 0.2979 0.3889 0.3856 0.1759 0.1637 0.3714 0.3784 0.4052 0.651 0.2934 0.4121 0.4505 0.5953
Cytoplasmic Foci 0.0204 0.0128 0.01 0.0036 0.0131 0.008 0.08 0.0376 0.0451 0.0508 0.0465 0.08 0.1337 0.02 0.0281 0.0052 0.0121 0.0168
Eisosomes 0.0006 0.0001 0.0002 0.0001 0.0002 0.0001 0.0004 0.0005 0.0002 0.0001 0 0 0.0033 0.0007 0.0002 0 0 0.0001
Endoplasmic Reticulum 0.0102 0.002 0.0081 0.0019 0.0029 0.0071 0.0207 0.0022 0.002 0.0005 0.0014 0.003 0.0092 0.0009 0.0005 0.0033 0.0021 0.0024
Endosome 0.0094 0.0033 0.0039 0.0073 0.0086 0.0461 0.0465 0.0156 0.0093 0.0167 0.0353 0.0768 0.025 0.0048 0.0096 0.0077 0.0052 0.0072
Golgi 0.0013 0.0006 0.0004 0.0011 0.0005 0.0008 0.011 0.0017 0.0015 0.0037 0.0025 0.0109 0.0089 0.0005 0.0071 0.0002 0.0009 0.0007
Lipid Particles 0.0194 0.0073 0.0052 0.0044 0.0144 0.0039 0.0363 0.0243 0.0134 0.076 0.0479 0.0175 0.0183 0.0032 0.0116 0.002 0.0089 0.0029
Mitochondria 0.018 0.0038 0.0041 0.0082 0.002 0.0033 0.0132 0.0051 0.0207 0.006 0.0027 0.005 0.0322 0.0017 0.0441 0.0005 0.0009 0.0018
None 0.01 0.0011 0.0029 0.002 0.0013 0.0026 0.0083 0.0019 0.0068 0.0025 0.0012 0.0014 0.0353 0.0017 0.0017 0.0004 0.0012 0.0014
Nuclear Periphery 0.1034 0.0243 0.0304 0.0955 0.1328 0.16 0.059 0.0165 0.0223 0.041 0.0532 0.061 0.0626 0.0064 0.0065 0.0136 0.0818 0.0272
Nucleolus 0.1369 0.2964 0.4257 0.2228 0.1305 0.0813 0.1138 0.3476 0.4903 0.3916 0.2497 0.2208 0.0744 0.1576 0.3656 0.2194 0.1419 0.2131
Nucleus 0.1214 0.1016 0.1005 0.1593 0.1265 0.1209 0.0603 0.0592 0.0939 0.0945 0.0777 0.0398 0.0525 0.0371 0.0608 0.036 0.2205 0.0869
Peroxisomes 0.0007 0.0003 0.0003 0.0003 0.0001 0.0002 0.0063 0.0006 0.002 0.0011 0.0079 0.0005 0.0144 0.0003 0.0061 0.0001 0.0001 0.0002
Punctate Nuclear 0.0088 0.0082 0.0047 0.004 0.0057 0.0476 0.0167 0.0046 0.0048 0.0126 0.0111 0.0084 0.0076 0.0088 0.001 0.0019 0.0152 0.0146
Vacuole 0.0921 0.0772 0.0961 0.0832 0.1092 0.1094 0.0582 0.0547 0.0583 0.0438 0.0469 0.0356 0.0336 0.0792 0.0976 0.2147 0.0266 0.0143
Vacuole Periphery 0.06 0.0261 0.0414 0.0489 0.106 0.1061 0.0362 0.0269 0.0293 0.0881 0.0419 0.0565 0.0102 0.0145 0.0349 0.0726 0.0292 0.0106

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1468.2035 1606.3841 2020.5353 1911.6127 1938.6254 1429.8807 1547.209 1483.6256 1560.5932 1579.2579
Translational Efficiency 1.2192 1.2341 1.1512 1.0583 1.2057 1.3463 1.2358 1.4271 1.2844 1.3847

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
211 286 247 1837 2125 1866 248 1034 2336 2152 495 2871

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2964.06 5795.68 8565.95 4534.47 5682.20 5628.58 5977.97 4976.72 5436.68 5650.79 7269.35 4693.75
Standard Deviation 903.49 1281.80 1487.53 1954.94 1310.33 1465.10 1638.83 2367.57 1497.56 1443.20 2030.80 2123.50
Intensity Change Log 2 — 0.967403 1.531039 0.613359 — -0.013679 0.073206 -0.191254 — 0.401953 0.750268 0.137550

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000399 0.000187 0.000144 0.000909 0.000057 0.000121 0.000487 0.000870 0.000088 0.000130 0.000316 0.000895
Bud Neck 0.012559 0.015302 0.015163 0.036063 0.002481 0.011037 0.044675 0.041503 0.003391 0.011603 0.029948 0.038022
Bud Site 0.012868 0.010500 0.002857 0.008048 0.004131 0.005541 0.007865 0.009747 0.004920 0.006200 0.005366 0.008660
Cell Periphery 0.007588 0.011167 0.018561 0.000425 0.004103 0.001423 0.005207 0.000621 0.004418 0.002718 0.011870 0.000496
Cytoplasm 0.380486* 0.481390* 0.385751* 0.013642 0.705839* 0.607476* 0.110354* 0.026023 0.676451* 0.590719* 0.247774* 0.018101
Cytoplasmic Foci 0.104254 0.123886 0.046934 0.034011 0.034950 0.049773 0.114937 0.028988 0.041210 0.059623 0.081004 0.032202
Eisosomes 0.000013 0.000007 0.000007 0.000126 0.000001 0.000003 0.000035 0.000095 0.000002 0.000003 0.000021 0.000115
Endoplasmic Reticulum 0.002016 0.000254 0.000228 0.000428 0.001249 0.000276 0.000270 0.000742 0.001318 0.000273 0.000249 0.000541
Endosome 0.009507 0.002385 0.001658 0.002024 0.002977 0.002855 0.003884 0.003855 0.003567 0.002793 0.002773 0.002683
Golgi 0.115085* 0.029397 0.049233 0.003392 0.045288 0.022078 0.028359 0.004183 0.051593 0.023050 0.038775 0.003677
Lipid Particles 0.025205 0.018668 0.024633 0.001752 0.010558 0.003765 0.017460 0.001571 0.011881 0.005746 0.021039 0.001687
Mitochondria 0.000628 0.000662 0.000402 0.007479 0.000294 0.001081 0.002260 0.007560 0.000325 0.001025 0.001333 0.007508
Mitotic Spindle 0.025764 0.023057 0.025822 0.051200 0.017830 0.047611 0.078164 0.038455 0.018547 0.044347 0.052046 0.046610
None 0.001662 0.006408 0.002999 0.001499 0.003052 0.005908 0.001851 0.002551 0.002927 0.005974 0.002424 0.001878
Nuclear Periphery 0.003542 0.003197 0.016594 0.017874 0.001687 0.007029 0.035703 0.023887 0.001855 0.006520 0.026168 0.020040
Nuclear Periphery Foci 0.068658 0.052131 0.089269 0.020100 0.059902 0.054024 0.099094 0.023710 0.060693 0.053773 0.094192 0.021400
Nucleolus 0.048476 0.011416 0.003413 0.556949* 0.000859 0.014796 0.143788 0.432942* 0.005160 0.014347 0.073742 0.512287*
Nucleus 0.030982 0.076563 0.058001 0.176748* 0.009231 0.091755 0.078011 0.287685* 0.011196 0.089736 0.068026 0.216703*
Peroxisomes 0.001389 0.000165 0.000109 0.020283 0.000055 0.000245 0.011761 0.013080 0.000176 0.000234 0.005947 0.017689
Vacuole 0.073415 0.103994 0.199832 0.023660 0.050433 0.043891 0.182401 0.023978 0.052509 0.051879 0.191099 0.023775
Vacuole Periphery 0.075504 0.029265 0.058390 0.023388 0.045023 0.029313 0.033435 0.027956 0.047776 0.029307 0.045887 0.025033

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.64 3.32 -3.57 -15.34 -22.18* -4.31 -8.58 -20.84 -20.71* -4.13 -2.92 -7.24 -25.75 -29.94* -13.69
Bud Neck -0.68 -1.23 -8.42 -7.48 -6.48 -13.56 -13.10 -22.63 -15.39 0.65 -12.91 -13.02 -32.02 -19.17 -2.62
Bud Site 0.70 5.97 1.18 0.13 -9.07 -5.18 -7.46 -9.22 -6.06 -0.62 -4.62 -4.17 -10.38 -5.67 -4.59
Cell Periphery -1.03 -4.45 5.67 3.95 8.80 7.76 0.41 9.88 4.05 6.42 3.87 -5.80 11.98 5.63 9.70
Cytoplasm -2.40 0.62 14.08 19.74 15.85 12.95 43.12 78.28* 54.42 5.03 12.07 29.53 82.42* 61.35 14.97
Cytoplasmic Foci -1.85 2.76 4.50 6.14 1.98 -5.54 -8.91 -0.44 4.50 8.62 -6.31 -7.90 0.12 6.64 8.04
Eisosomes 2.67 2.45 -13.32 -15.06 -15.07 -9.18 -9.68 -17.93 -17.25 -7.10 -4.74 -9.94 -20.95 -20.53 -15.19
Endoplasmic Reticulum 3.83 3.92 3.43 -3.91 -5.40 4.92 5.00 2.50 -1.91 -1.99 5.70 5.93 4.29 -3.34 -4.00
Endosome 5.75 6.97 6.58 0.34 -1.68 -0.35 -2.23 -0.28 -0.08 1.39 1.60 1.72 2.54 1.27 0.90
Golgi 7.97 6.15 10.55 8.84 13.70 12.38 10.34 23.71 13.55 8.83 14.32 7.21 25.93 16.64 15.62
Lipid Particles 1.15 -0.34 5.11 4.27 7.87 7.72 -2.06 10.61 5.47 6.63 6.03 -4.30 11.70 6.51 10.12
Mitochondria -0.28 1.25 -18.44 -19.40 -21.25* -12.98 -7.75 -10.39 -8.68 -5.96 -12.60 -7.57 -22.47 -19.09 -16.23
Mitotic Spindle 0.24 -0.53 -4.42 -4.56 -3.32 -8.85 -5.86 -6.20 0.80 2.89 -8.39 -5.56 -10.34 -1.32 0.27
None -4.66 -3.60 0.05 4.75 4.27 -9.33 2.74 0.81 10.86 -2.24 -10.10 1.19 3.83 15.07 2.39
Nuclear Periphery 0.62 -6.01 -7.23 -8.64 0.78 -9.22 -8.16 -8.14 -5.33 2.68 -8.94 -10.78 -13.24 -8.22 2.95
Nuclear Periphery Foci 1.96 -1.53 6.08 5.13 7.99 1.49 -1.20 11.17 9.65 6.43 1.97 -2.74 13.70 11.82 10.23
Nucleolus 3.39 4.12 -32.39* -56.59* -62.45* -10.56 -8.04 -35.15* -32.39* -12.96 -7.16 -7.09 -70.13* -66.01* -37.98*
Nucleus -4.66 -3.82 -17.58 -7.70 -9.80 -22.09 -9.68 -32.48 -19.31 -14.51 -22.70 -11.91 -44.79 -18.79 -15.91
Peroxisomes 2.59 2.55 -15.82 -20.57* -20.49* -7.08 -5.19 -13.35 -12.94 -0.11 -2.20 -4.97 -24.08 -23.91 -8.40
Vacuole -2.28 -7.57 5.43 6.93 11.84 1.51 -7.95 5.56 4.19 9.46 -0.15 -12.44 8.51 8.23 15.13
Vacuole Periphery 4.27 2.00 4.89 0.99 5.49 6.89 6.40 7.38 1.28 -0.29 8.01 3.20 10.17 1.88 4.26